LOCUS Exported 7400 bp ds-DNA circular UNA 27-SEP-2018 DEFINITION natural circular DNA ACCESSION . VERSION . KEYWORDS . SOURCE natural DNA sequence ORGANISM unspecified REFERENCE 1 (bases 1 to 7400) AUTHORS . TITLE Direct Submission JOURNAL Exported Sep 27, 2018 from SnapGene 4.2.4 http://www.snapgene.com FEATURES Location/Qualifiers source 1..7400 /organism="unspecified" /mol_type="genomic DNA" CDS 5..67 /codon_start=1 /label=T2A optimized for BE3 /translation="GSGEGRGSLLTCGDVEENPGP" CDS 68..1030 /codon_start=1 /label=BirA* /translation="KDNTVPLKLIALLANGEFHSGEQLGETLGMSRAAINKHIQTLRDW GVDVFTVPGKGYSLPEPIQLLNAKQILGQLDGGSVAVLPVIDSTNQYLLDRIGELKSGD ACIAEYQQAGRGGRGRKWFSPFGANLYLSMFWRLEQGPAAAIGLSLVIGIVMAEVLRKL GADKVRVKWPNDLYLQDRKLAGILVELTGKTGDAAQIVIGAGINMAMRRVEESVVNQGW ITLQEAGINLDRNTLAAMLIRELRAALELFEQEGLAPYLSRWEKLDNFINRPVKLIIGD KEIFGISRGIDKQGALLLEQDGIIKPWMGGEISLRSAEK" region 1028..1034 /label=GGS linker /note="/region_type=Kinase" CDS 1077..2759 /codon_start=1 /label=Floxed NeoR Cassette /translation="ITSYSIHYTKLSRPTG*GRRFSQGSLEHAL*QPRWALGATQVASG LAHIPHPPVGANRLRSLVAPSRHLLLLP*SGSSPPPRSSRRAGRDKWK*HVSLVSCRWT APLSNGSG*AFGAAANSSFGSFAFWAQRLGRGGSGGGLRGGLRGGAGARRSSGSPAFCT LQKRTSAALFSSSSSPGLSTCSQYGIGH*TRWIARRFSGRLGGEAIRL*LGTTDNRLL* CRRVPAVSAGAPGSFCQDRPVRCPE*TAGRGSAAIVAGHDGRSLRSCARRCH*SGKGLA AIGRSAGAGSPVISPCSCRESIHHG*CNAAAAYA*SGYLPIRPPSETSHRASTYSDGSR SCQSG*SGRRASGARASRTVRQAQGAHARRRGSRRDPWRCLLAEYHGGKWPLFWIHRLW PAGCGGSLSGHSVGYP*YC*RAWRRMG*PLPRALRYRRSRFAAHRLLSPS*RVLLRGSI L*SSLISLDCAF*LPAICCLPLPRAFLDPGRCHSHCPFLIK*GNCIALSE*VSFYSGGW GGAGQQGGGLGRQ*QACWGCGGLYGF*GGKNQL*LRIAYIIRSY" misc_feature 1077..1110 /label=LoxSite /note="/feature_type=Complex" region 2648..2667 /label=O-130 /note="/region_type=Homeobox" misc_feature complement(2726..2759) /label=LoxSite /note="/feature_type=Complex" gene 2793..3625 /label=TP53 HR2 gene 6518..7400 /label=TP53 HR1 ORIGIN 1 tatgggcagt ggagagggca gaggaagtct gctaacctgc ggggacgtcg aggagaatcc 61 tggcccaaag gacaacacgg tgcccctgaa gctgatcgcc ctgctggcca acggcgagtt 121 ccactctggc gagcagctgg gagagaccct gggaatgagc agagccgcca tcaacaagca 181 catccagaca ctgagagact ggggagtgga cgtgttcacc gtgcctggca agggctacag 241 cctgcctgag cctatccagc tgctgaacgc caagcagatc ctgggacagc tggatggcgg 301 aagcgtggcc gtgctgcctg tgatcgactc caccaatcag tacctgctgg acagaatcgg 361 agagctgaag tccggcgacg cctgcatcgc cgagtaccag caggctggca gaggaggcag 421 aggacggaag tggttcagcc cattcggagc caacctgtac ctgtccatgt tctggagact 481 ggagcaggga cctgctgctg ccatcggact gagtctggtg atcggaatcg tgatggccga 541 ggtgctgaga aagctgggag ccgacaaggt gagagtgaag tggcctaatg acctgtacct 601 ccaggaccgc aagctggctg gcatcctggt ggagctgaca ggcaagacag gcgatgccgc 661 tcagatcgtg atcggagccg gaatcaacat ggccatgaga agagtggagg agagcgtggt 721 gaaccagggc tggatcaccc tgcaggaggc tggcatcaac ctggaccgga acaccctggc 781 cgccatgctg atcagagagc tgagagccgc tctggagctg ttcgagcagg agggactggc 841 tccttacctg agcagatggg agaagctgga caacttcatc aacagacctg tgaagctgat 901 catcggcgac aaggaaatct tcggcatctc cagaggaatc gacaagcagg gagctctgct 961 gctggagcag gacggaatca tcaagccctg gatgggcgga gaaatctccc tgagaagcgc 1021 agagaagtaa tgaaagcttg aagttcctat tctctagaaa gtataggaac ttccggataa 1081 cttcgtatag catacattat acgaagttat cgcgccctac cgggtagggg aggcgctttt 1141 cccaaggcag tctggagcat gcgctttagc agccccgctg ggcacttggc gctacacaag 1201 tggcctctgg cctcgcacac attccacatc caccggtagg cgccaaccgg ctccgttctt 1261 tggtggcccc ttcgcgccac cttctactcc tcccctagtc aggaagttcc cccccgcccc 1321 gcagctcgcg tcgtgcagga cgtgacaaat ggaagtagca cgtctcacta gtctcgtgca 1381 gatggacagc accgctgagc aatggaagcg ggtaggcctt tggggcagcg gccaatagca 1441 gctttggctc cttcgctttc tgggctcaga ggctgggaag gggtgggtcc gggggcgggc 1501 tcaggggcgg gctcaggggc ggggcgggcg cccgaaggtc ctccggaagc ccggcattct 1561 gcacgcttca aaagcgcacg tctgccgcgc tgttctcctc ttcctcatct ccgggccttt 1621 cgacctgcag ccaatatggg atcggccatt gaacaagatg gattgcacgc aggttctccg 1681 gccgcttggg tggagaggct attcggctat gactgggcac aacagacaat cggctgctct 1741 gatgccgccg tgttccggct gtcagcgcag gggcgcccgg ttctttttgt caagaccgac 1801 ctgtccggtg ccctgaatga actgcaggac gaggcagcgc ggctatcgtg gctggccacg 1861 acgggcgttc cttgcgcagc tgtgctcgac gttgtcactg aagcgggaag ggactggctg 1921 ctattgggcg aagtgccggg gcaggatctc ctgtcatctc accttgctcc tgccgagaaa 1981 gtatccatca tggctgatgc aatgcggcgg ctgcatacgc ttgatccggc tacctgccca 2041 ttcgaccacc aagcgaaaca tcgcatcgag cgagcacgta ctcggatgga agccggtctt 2101 gtcaatcagg atgatctgga cgaagagcat caggggctcg cgccagccga actgttcgcc 2161 aggctcaagg cgcgcatgcc cgacggcgag gatctcgtcg tgacccatgg cgatgcctgc 2221 ttgccgaata tcatggtgga aaatggccgc ttttctggat tcatcgactg tggccggctg 2281 ggtgtggcgg atcgctatca ggacatagcg ttggctaccc gtgatattgc tgaagagctt 2341 ggcggcgaat gggctgaccg cttcctcgtg ctttacggta tcgccgctcc cgattcgcag 2401 cgcatcgcct tctatcgcct tcttgacgag ttcttctgag gggatcaatt ctctagagct 2461 cgctgatcag cctcgactgt gccttctagt tgccagccat ctgttgtttg cccctccccc 2521 gtgccttcct tgaccctgga aggtgccact cccactgtcc tttcctaata aaatgaggaa 2581 attgcatcgc attgtctgag taggtgtcat tctattctgg ggggtggggt ggggcaggac 2641 agcaaggggg aggattggga agacaatagc aggcatgctg gggatgcggt gggctctatg 2701 gcttctgagg cggaaagaac cagctataac ttcgtatagc atacattata cgaagttata 2761 gtaatcgata actcgagaag gatccaaggt acccattctc cacttcttgt tccccactga 2821 cagcctccca cccccatctc tccctcccct gccattttgg gttttgggtc tttgaaccct 2881 tgcttgcaat aggtgtgcgt cagaagcacc caggacttcc atttgctttg tcccggggct 2941 ccactgaaca agttggcctg cactggtgtt ttgttgtggg gaggaggatg gggagtagga 3001 cataccagct tagattttaa ggtttttact gtgagggatg tttgggagat gtaagaaatg 3061 ttcttgcagt taagggttag tttacaatca gccacattct aggtaggggc ccacttcacc 3121 gtactaacca gggaagctgt ccctcactgt tgaattttct ctaacttcaa ggcccatatc 3181 tgtgaaatgc tggcatttgc acctacctca cagagtgcat tgtgagggtt aatgaaataa 3241 tgtacatctg gccttgaaac caccttttat tacatggggt ctagaacttg acccccttga 3301 gggtgcttgt tccctctccc tgttggtcgg tgggttggta gtttctacag ttgggcagct 3361 ggttaggtag agggagttgt caagtctctg ctggcccagc caaaccctgt ctgacaacct 3421 cttggtgaac cttagtacct aaaaggaaat ctcaccccat cccacaccct ggaggatttc 3481 atctcttgta tatgatgatc tggatccacc aagacttgtt ttatgctcag ggtcaatttc 3541 ttttttcttt tttttttttt ctttttcttt gagactgggt ctcgctttgt tgcccaggct 3601 ggagtggagt ggcgtgatct gctagcaaaa gcggccgcag gaacccctag tgatggagtt 3661 ggccactccc tctctgcgcg ctcgctcgct cactgaggcc gggcgaccaa aggtcgcccg 3721 acgcccgggc tttgcccggg cggcctcagt gagcgagcga gcgcgcagct gcctgcaggg 3781 gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatacgtca 3841 aagcaaccat agtacgcgcc ctgtagcggc gcattaagcg cggcgggtgt ggtggttacg 3901 cgcagcgtga ccgctacact tgccagcgcc ttagcgcccg ctcctttcgc tttcttccct 3961 tcctttctcg ccacgttcgc cggctttccc cgtcaagctc taaatcgggg gctcccttta 4021 gggttccgat ttagtgcttt acggcacctc gaccccaaaa aacttgattt gggtgatggt 4081 tcacgtagtg ggccatcgcc ctgatagacg gtttttcgcc ctttgacgtt ggagtccacg 4141 ttctttaata gtggactctt gttccaaact ggaacaacac tcaactctat ctcgggctat 4201 tcttttgatt tataagggat tttgccgatt tcggtctatt ggttaaaaaa tgagctgatt 4261 taacaaaaat ttaacgcgaa ttttaacaaa atattaacgt ttacaatttt atggtgcact 4321 ctcagtacaa tctgctctga tgccgcatag ttaagccagc cccgacaccc gccaacaccc 4381 gctgacgcgc cctgacgggc ttgtctgctc ccggcatccg cttacagaca agctgtgacc 4441 gtctccggga gctgcatgtg tcagaggttt tcaccgtcat caccgaaacg cgcgagacga 4501 aagggcctcg tgatacgcct atttttatag gttaatgtca tgataataat ggtttcttag 4561 acgtcaggtg gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt atttttctaa 4621 atacattcaa atatgtatcc gctcatgaga caataaccct gataaatgct tcaataatat 4681 tgaaaaagga agagtatgag tattcaacat ttccgtgtcg cccttattcc cttttttgcg 4741 gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa 4801 gatcagttgg gtgcacgagt gggttacatc gaactggatc tcaacagcgg taagatcctt 4861 gagagttttc gccccgaaga acgttttcca atgatgagca cttttaaagt tctgctatgt 4921 ggcgcggtat tatcccgtat tgacgccggg caagagcaac tcggtcgccg catacactat 4981 tctcagaatg acttggttga gtactcacca gtcacagaaa agcatcttac ggatggcatg 5041 acagtaagag aattatgcag tgctgccata accatgagtg ataacactgc ggccaactta 5101 cttctgacaa cgatcggagg accgaaggag ctaaccgctt ttttgcacaa catgggggat 5161 catgtaactc gccttgatcg ttgggaaccg gagctgaatg aagccatacc aaacgacgag 5221 cgtgacacca cgatgcctgt agcaatggca acaacgttgc gcaaactatt aactggcgaa 5281 ctacttactc tagcttcccg gcaacaatta atagactgga tggaggcgga taaagttgca 5341 ggaccacttc tgcgctcggc ccttccggct ggctggttta ttgctgataa atctggagcc 5401 ggtgagcgtg ggtctcgcgg tatcattgca gcactggggc cagatggtaa gccctcccgt 5461 atcgtagtta tctacacgac ggggagtcag gcaactatgg atgaacgaaa tagacagatc 5521 gctgagatag gtgcctcact gattaagcat tggtaactgt cagaccaagt ttactcatat 5581 atactttaga ttgatttaaa acttcatttt taatttaaaa ggatctaggt gaagatcctt 5641 tttgataatc tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac 5701 cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc 5761 ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca 5821 actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgttcttcta 5881 gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct 5941 ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg 6001 gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc 6061 acacagccca gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta 6121 tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg 6181 gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt 6241 cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg 6301 cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg 6361 ccttttgctc acatgtcctg caggcagctg cgcgctcgct cgctcactga ggccgcccgg 6421 gcgtcgggcg acctttggtc gcccggcctc agtgagcgag cgagcgcgca gagagggagt 6481 ggccaactcc atcactaggg gttcctgcgg ccgcaaacgc gtcagccctg cacagacatt 6541 tttttagtct tcctccggtt gaatcctata accacattct tgcctcagtg tatccacaga 6601 acatccaaac ccagggacga gtgtggatac ttctttgcca ttctccgcaa ctcccagccc 6661 agagctggag ggtctcaagg aggggcctaa taattgtgta atactgaata cagccagagt 6721 ttcaggtcat atactcagcc ctgccatgca ccggcaggtc ctaggtgacc cccgtcaaac 6781 tcagtttcct tatatataaa atggggtaag ggggccgggc gcagtggctc acgaatccca 6841 cactctggga ggccaaggcg agtggatcac ctgaggtcgg gagtttgagc ccagcctgac 6901 caacatggag aaaccccatc tctactaaaa atacaaaagt agccgggcgt ggtgatgcat 6961 gcctgtaatc ccagctacct actcgggagg ctgaggcagg agaatcgctt gaacccggga 7021 ggcagaggtt gcggtgagct gagatctcac cattacactc cagcctgggc aacaagagtg 7081 aaactccgtc tcaaaaaaga taaataaagt aaaatggggt aagggaagat tacgagacta 7141 atacacacta atactctgag gtgctcagta aacatatttg catggggtgt ggccaccatc 7201 ttgatttgaa ttcccgttgt cccagcctta ggcccttcaa agcattggtc agggaaaagg 7261 ggcacagacc ctctcactca tgtgatgtca tctctcctcc ctgcttctgt ctcctacagc 7321 cacctgaagt ccaaaaaggg tcagtctacc tcccgccata aaaaactcat gttcaagaca 7381 gaagggcctg actcagacca //