LOCUS       Exported                5335 bp ds-DNA     circular SYN 13-AUG-2018
DEFINITION  .
ACCESSION   .
VERSION     .
KEYWORDS    pKEW321-MbCas12a-RFP-GFP-array
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5335)
  AUTHORS   Kyle Watters
  TITLE     Direct Submission
  JOURNAL   Exported Aug 13, 2018 from SnapGene 4.2.3
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..5335
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
                     /note="color: #ffffff"
     misc_feature    16..53
                     /label=P_cat
                     /note="ApEinfo_revcolor=#f0a831 ApEinfo_fwdcolor=#f0a831"
                     /note="color: #f0a831"
     misc_feature    62..73
                     /label=RBS(strong)
                     /note="ApEinfo_revcolor=#884946 ApEinfo_fwdcolor=#884946"
                     /note="color: #884946"
     gene            80..1207
                     /label=LacI
                     /note="ApEinfo_revcolor=#d94454 ApEinfo_fwdcolor=#d94454"
                     /note="color: #d94454; direction: RIGHT"
     misc_feature    complement(1226..1231)
                     /label=BamHI-BglII scar(3)
                     /note="ApEinfo_label=BamHI-BglII scar 
                     ApEinfo_revcolor=#f109ff ApEinfo_fwdcolor=#f109ff"
                     /note="color: #f109ff; direction: LEFT"
     terminator      1241..1369
                     /label=dblTerm ApEinfo_revcolor=#841661
                     /label=dblTerm
                     /note="ApEinfo_revcolor=#841661 ApEinfo_fwdcolor=#841661"
                     /note="color: #841661"
     terminator      1241..1308
                     /label=sglTerm-1 ApEinfo_revcolor=#8416d8
                     /label=sglTerm-1
                     /note="ApEinfo_revcolor=#8416d8 ApEinfo_fwdcolor=#8416d8"
                     /note="color: #8416d8"
     promoter        1448..1501
                     /label=p_Llac0-1 (BBa_R0011)
                     /label=p_Llac0-1
                     /note="ApEinfo_revcolor=#f0a831 ApEinfo_fwdcolor=#f0a831 
                     Based on the left lambda promoter (PL lambda promoter)"
                     /note="color: #f0a831"
     misc_feature    1502..1537
                     /label=M. bovoculi CRISPR repeat
                     /note="color: #a6acb3"
     misc_feature    1538..1561
                     /label=RFP target 1
                     /note="color: #ff00ff"
     misc_feature    1562..1597
                     /label=M. bovoculi CRISPR repeat
                     /note="color: #a6acb3"
     misc_feature    1598..1621
                     /locus_tag="SFGFP"
                     /label=MbCas12 target 4
                     /note="ApEinfo_fwdcolor=#00f700 
                     ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} 
                     width 5 offset 0 ApEinfo_revcolor=green"
                     /note="color: #00f700"
     misc_feature    1622..1657
                     /label=M. bovoculi CRISPR repeat
                     /note="color: #a6acb3"
     misc_feature    1658..1687
                     /label=t500
                     /note="ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 
                     0} width 5 offset 0 ApEinfo_revcolor=green 
                     ApEinfo_fwdcolor=cyan"
                     /note="color: #00ffff"
     CDS             complement(1741..2619)
                     /codon_start=1
                     /gene="araC"
                     /product="L-arabinose regulatory protein"
                     /label=araC
                     /note="color: #993366"
                     /translation="MAEAQNDPLLPGYSFNAHLVAGLTPIEANGYLDFFIDRPLGMKGY
                     ILNLTIRGQGVVKNQGREFVCRPGDILLFPPGEIHHYGRHPEAREWYHQWVYFRPRAYW
                     HEWLNWPSIFANTGFFRPDEAHQPHFSDLFGQIINAGQGEGRYSELLAINLLEQLLLRR
                     MEAINESLHPPMDNRVREACQYISDHLADSNFDIASVAQHVCLSPSRLSHLFRQQLGIS
                     VLSWREDQRISQAKLLLSTTRMPIATVGRNVGFDDQLYFSRVFKKCTGASPSEFRAGCE
                     EKVNDVAVKLS"
     promoter        2646..2930
                     /gene="araBAD"
                     /label=araBAD promoter
                     /note="promoter of the L-arabinose operon of E. coli; the 
                     araC regulatory gene is transcribed in the opposite 
                     direction (Guzman et al., 1995)"
                     /note="color: #ffffff"
     terminator      3145..3231
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
                     /note="color: #ffffff"
     terminator      3323..3350
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
                     /note="color: #ffffff"
     rep_origin      complement(3458..4140)
                     /direction=LEFT
                     /label=ColE1 origin
                     /note="ApEinfo_revcolor=gray50 ApEinfo_fwdcolor=gray50"
                     /note="color: #808080"
     misc_feature    4056..4061
                     /label=BamHI-BglII scar
                     /note="ApEinfo_revcolor=#f109ff ApEinfo_fwdcolor=#f109ff"
                     /note="color: #f109ff"
     misc_feature    complement(4067..4072)
                     /label=BamHI-BglII scar(4)
                     /note="ApEinfo_label=BamHI-BglII scar 
                     ApEinfo_revcolor=#f109ff ApEinfo_fwdcolor=#f109ff"
                     /note="color: #f109ff; direction: LEFT"
     misc_feature    4153..4158
                     /label=BamHI-BglII scar(1)
                     /note="ApEinfo_label=BamHI-BglII scar 
                     ApEinfo_revcolor=#f109ff ApEinfo_fwdcolor=#f109ff"
                     /note="color: #f109ff"
     misc_feature    complement(4165..4170)
                     /label=BamHI-BglII scar(5)
                     /note="ApEinfo_label=BamHI-BglII scar 
                     ApEinfo_revcolor=#f109ff ApEinfo_fwdcolor=#f109ff"
                     /note="color: #f109ff; direction: LEFT"
     CDS             complement(4238..4897)
                     /label=AmpR
                     /note="ApEinfo_revcolor=yellow ApEinfo_fwdcolor=yellow"
                     /note="color: #ffff00; direction: LEFT"
     misc_feature    4497..4502
                     /label=NheI-XbaI scar
                     /note="ApEinfo_revcolor=#f109ff ApEinfo_fwdcolor=#f109ff"
                     /note="color: #f109ff"
     misc_feature    4933..4938
                     /label=BamHI-BglII scar(2)
                     /note="ApEinfo_label=BamHI-BglII scar 
                     ApEinfo_revcolor=#f109ff ApEinfo_fwdcolor=#f109ff"
                     /note="color: #f109ff"
     misc_feature    complement(4950..4955)
                     /label=BamHI-BglII scar(6)
                     /note="ApEinfo_label=BamHI-BglII scar 
                     ApEinfo_revcolor=#f109ff ApEinfo_fwdcolor=#f109ff"
                     /note="color: #f109ff; direction: LEFT"
ORIGIN
        1 gaattctaaa gatctggcac gtaagaggtt ccaactttca ccataatgaa acatactaga
       61 gaaagaggag aaatactaga tggtgaatgt gaaaccagta acgttatacg atgtcgcaga
      121 gtatgccggt gtctcttatc agaccgtttc ccgcgtggtg aaccaggcca gccacgtttc
      181 tgcgaaaacg cgggaaaaag tggaagcggc gatggcggag ctgaattaca ttcccaaccg
      241 cgtggcacaa caactggcgg gcaaacagtc gttgctgatt ggcgttgcca cctccagtct
      301 ggccctgcac gcgccgtcgc aaattgtcgc ggcgattaaa tctcgcgccg atcaactggg
      361 tgccagcgtg gtggtgtcga tggtagaacg aagcggcgtc gaagcctgta aagcggcggt
      421 gcacaatctt ctcgcgcaac gcgtcagtgg gctgatcatt aactatccgc tggatgacca
      481 ggatgccatt gctgtggaag ctgcctgcac taatgttccg gcgttatttc ttgatgtctc
      541 tgaccagaca cccatcaaca gtattatttt ctcccatgaa gacggtacgc gactgggcgt
      601 ggagcatctg gtcgcattgg gtcaccagca aatcgcgctg ttagcgggcc cattaagttc
      661 tgtctcggcg cgtctgcgtc tggctggctg gcataaatat ctcactcgca atcaaattca
      721 gccgatagcg gaacgggaag gcgactggag tgccatgtcc ggttttcaac aaaccatgca
      781 aatgctgaat gagggcatcg ttcccactgc gatgctggtt gccaacgatc agatggcgct
      841 gggcgcaatg cgcgccatta ccgagtccgg gctgcgcgtt ggtgcggata tctcggtagt
      901 gggatacgac gataccgaag acagctcatg ttatatcccg ccgttaacca ccatcaaaca
      961 ggattttcgc ctgctggggc aaaccagcgt ggaccgcttg ctgcaactct ctcagggcca
     1021 ggcggtgaag ggcaatcagc tgttgcccgt ctcactggtg aaaagaaaaa ccaccctggc
     1081 gcccaatacg caaaccgcct ctccccgcgc gttggccgat tcattaatgc agctggcacg
     1141 acaggtttcc cgactggaaa gcgggcaggc tgcaaacgac gaaaactacg ctttagtagc
     1201 ttaataactc tgatagtgct agtgtagatc cctactagag ccaggcatca aataaaacga
     1261 aaggctcagt cgaaagactg ggcctttcgt tttatctgtt gtttgtcggt gaacgctctc
     1321 tactagagtc acactggctc accttcgggt gggcctttct gcgtttatat attgcttaga
     1381 ataatcgatc tgcggccgca gagagtgtag cttacctagt catcgaaagc tttgctacag
     1441 cggatagaat tgtgagcgga taacaattga cattgtgagc ggataacaag atactactag
     1501 tgtctaacga ccttttaaat ttctactgtt tgtagataaa gttcgtatgg aaggttccgt
     1561 tgtctaacga ccttttaaat ttctactgtt tgtagattgc ccattaacat caccatctaa
     1621 tgtctaacga ccttttaaat ttctactgtt tgtagatcaa agcccgccga aaggcgggct
     1681 tttttttgtg gatatacctt actcgagtta gccttgatag attgtctgat tcgttaccaa
     1741 ttatgacaac ttgacggcta catcattcac tttttcttca caaccggcac ggaactcgct
     1801 cgggctggcc ccggtgcatt ttttaaatac ccgcgagaaa tagagttgat cgtcaaaacc
     1861 aacattgcga ccgacggtgg cgataggcat ccgggtggtg ctcaaaagca gcttcgcctg
     1921 gctgatacgt tggtcctcgc gccagcttaa gacgctaatc cctaactgct ggcggaaaag
     1981 atgtgacaga cgcgacggcg acaagcaaac atgctgtgcg acgctggcga tatcaaaatt
     2041 gctgtctgcc aggtgatcgc tgatgtactg acaagcctcg cgtacccgat tatccatcgg
     2101 tggatggagc gactcgttaa tcgcttccat gcgccgcagt aacaattgct caagcagatt
     2161 tatcgccagc agctccgaat agcgcccttc cccttgcccg gcgttaatga tttgcccaaa
     2221 caggtcgctg aaatgcggct ggtgcgcttc atccgggcga aagaaccccg tattggcaaa
     2281 tattgacggc cagttaagcc attcatgcca gtaggcgcgc ggacgaaagt aaacccactg
     2341 gtgataccat tcgcgagcct ccggatgacg accgtagtga tgaatctctc ctggcgggaa
     2401 cagcaaaata tcacccggtc ggcaaacaaa ttctcgtccc tgatttttca ccaccccctg
     2461 accgcgaatg gtgagattga gaatataacc tttcattccc agcggtcggt cgataaaaaa
     2521 atcgagataa ccgttggcct caatcggcgt taaacccgcc accagatggg cattaaacga
     2581 gtatcccggc agcaggggat cattttgcgc ttcagccata cttttcatac tcccgccatt
     2641 cagagaagaa accaattgtc catattgcat cagacattgc cgtcactgcg tcttttactg
     2701 gctcttctcg ctaaccaaac cggtaacccc gcttattaaa agcattctgt aacaaagcgg
     2761 gaccaaagcc atgacaaaaa cgcgtaacaa aagtgtctat aatcacggca gaaaagtcca
     2821 cattgattat ttgcacggcg tcacactttg ctatgccata gcatttttat ccataagatt
     2881 agcggatcct acctgacgct ttttatcgca actctctact gtttctccat atatcggatc
     2941 cttagtaaac ctgcaggcac tgcccatgga cctcggtacc gaatagctag ccggtaatgc
     3001 attcgctaga gctcctaaag catgcgacct gcaaccggtc tgtcacgtac gtcgccaccg
     3061 tcgacgtcgt tcgtaagtag cctagataaa taaaataatc agttaaccgc gagccccatg
     3121 cgagagtagg gaactgccag gcatcaaata aaacgaaagg ctcagtcgaa agactgggcc
     3181 tttcgtttta tctgttgttt gtcggtgaac gctctcctga gtaggacaaa tccgccggga
     3241 gcggatttga acgttgcgaa gcaacggccc ggagggtggc gggcaggacg cccgccataa
     3301 actgccaggc atcaaattaa gcagaaggcc atcctgacgg atggcctttt tgcgtttcta
     3361 caaactctgc ggtaatacgg ttatccacag aatcagggga taacgcagga aagaacatgt
     3421 gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc
     3481 ataggctccg cccccctgac gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa
     3541 acccgacagg actataaaga taccaggcgt ttccccctgg aagctccctc gtgcgctctc
     3601 ctgttccgac cctgccgctt accggatacc tgtccgcctt tctcccttcg ggaagcgtgg
     3661 cgctttctca tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc
     3721 tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc ggtaactatc
     3781 gtcttgagtc caacccggta agacacgact tatcgccact ggcagcagcc actggtaaca
     3841 ggattagcag agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg tggcctaact
     3901 acggctacac tagaaggaca gtatttggta tctgcgctct gctgaagcca gttaccttcg
     3961 gaaaaagagt tggtagctct tgatccggca aacaaaccac cgctggtagc ggtggttttt
     4021 ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat cctttgatct
     4081 tttctacggg gtctgacgct cagtggaacg aaaactcacg ttaagggatt ttggtcatga
     4141 gattatcaaa aaggatcttc acctagatcc ttttaaatta aaaatgaagt tttaaatcaa
     4201 tctaaagtat atatgagtaa acttggtctg acagttacca atgcttaatc agtgaggcac
     4261 ctatctcagc gatctgtcta tttcgttcat ccatagttgc ctgactcccc gtcgtgtaga
     4321 taactacgat acgggagggc ttaccatctg gccccagtgc tgcaatgata ccgcgagacc
     4381 cacgctcacc ggctccagat ttatcagcaa taaaccagcc agccggaagg gccgagcgca
     4441 gaagtggtcc tgcaacttta tccgcctcca tccagtctat taattgttgc cgggaagcta
     4501 gagtaagtag ttcgccagtt aatagtttgc gcaacgttgt tgccattgct acaggcatcg
     4561 tggtgtcacg ctcgtcgttt ggtatggctt cattcagctc cggttcccaa cgatcaaggc
     4621 gagttacatg atcccccatg ttgtgcaaaa aagcggttag ctccttcggt cctccgatcg
     4681 ttgtcagaag taagttggcc gcagtgttat cactcatggt tatggcagca ctgcataatt
     4741 ctcttactgt catgccatcc gtaagatgct tttctgtgac tggtgagtac tcaaccaagt
     4801 cattctgaga atagtgtatg cggcgaccga gttgctcttg cccggcgtca atacgggata
     4861 ataccgcgcc acatagcaga actttaaaag tgctcatcat tggaaaacgt tcttcggggc
     4921 gaaaactctc aaggatctta ccgctgttga gatccagttc gatgtaaccc actcgtgcac
     4981 ccaactgatc ttcagcatct tttactttca ccagcgtttc tgggtgagca aaaacaggaa
     5041 ggcaaaatgc cgcaaaaaag ggaataaggg cgacacggaa atgttgaata ctcatactct
     5101 tcctttttca atattattga agcatttatc agggttattg tctcatgagc ggatacatat
     5161 ttgaatgtat ttagaaaaat aaacaaatag gggttccgcg cacatttccc cgaaaagtgc
     5221 cacctgacgt ctaagaaacc attattatca tgacattaac ctataaaaat aggcgtatca
     5281 cgaggcagaa tttcagataa aaaaaatcct tagctttcgc taaggatgat ttctg
//

Y2Unique 6+ CuttersHOdEÿÿÿÿYô?