LOCUS       PB-CAG-GFPd2            6466 bp ds-DNA     circular     10-AUG-2018
DEFINITION  .
FEATURES             Location/Qualifiers
     terminator      4289..4420
                     /label="SV40_PA_terminator"
                     /ApEinfo_revcolor=#9eafd2
                     /ApEinfo_fwdcolor=#9eafd2
     promoter        complement(3908..3926)
                     /label="M13_reverse_primer"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
     gene            2135..2848
                     /label="EGFP"
                     /ApEinfo_revcolor=#b7e6d7
                     /ApEinfo_fwdcolor=#b7e6d7
     promoter        complement(3961..3990)
                     /label="lac_promoter"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
     promoter        complement(6320..6348)
                     /label="AmpR_promoter"
                     /ApEinfo_revcolor=#9eafd2
                     /ApEinfo_fwdcolor=#9eafd2
     misc_feature    complement(2177..2198)
                     /label="EGFP_N_primer"
                     /ApEinfo_revcolor=#b7e6d7
                     /ApEinfo_fwdcolor=#b7e6d7
     gene            complement(5418..6278)
                     /label="Ampicillin"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
     feature         complement(314..347)
                     /label="loxP"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
     primer          4377..4396
                     /label="EBV_rev_primer"
                     /ApEinfo_revcolor=#c6c9d1
                     /ApEinfo_fwdcolor=#c6c9d1
     misc_feature    3855..3889
                     /label="5' ITR specific sequence"
                     /ApEinfo_revcolor=#d6b295
                     /ApEinfo_fwdcolor=#d6b295
     misc_feature    2785..2806
                     /label="EGFP_C_primer"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     primer          complement(3925..3947)
                     /label="M13_pUC_rev_primer"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     misc_feature    join(6421..6466,1..17)
                     /label="3' ITR specific sequence"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     misc_feature    join(6417..6466,1..380)
                     /label="3' Terminal Repeat"
                     /ApEinfo_revcolor=#d6b295
                     /ApEinfo_fwdcolor=#d6b295
     misc_feature    2253..2272
                     /label="siGFP target"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     origin          complement(4644..5263)
                     /label="pBR322_origin"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     origin          4131..4208
                     /label="SV40_origin"
                     /ApEinfo_revcolor=#75c6a9
                     /ApEinfo_fwdcolor=#75c6a9
     misc_feature    2315..2344
                     /label="Y66 (EGFP)"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     primer          2041..2060
                     /label="pCAG_F_primer"
                     /ApEinfo_revcolor=#c7b0e3
                     /ApEinfo_fwdcolor=#c7b0e3
     promoter        4096..4275
                     /label="SV40_promoter"
                     /ApEinfo_revcolor=#b4abac
                     /ApEinfo_fwdcolor=#b4abac
     terminator      2999..3500
                     /label="rb_glob_PA_terminator"
                     /ApEinfo_revcolor=#9eafd2
                     /ApEinfo_fwdcolor=#9eafd2
     misc_feature    381..2082
                     /label="CAG Promoter"
                     /ApEinfo_revcolor=#d59687
                     /ApEinfo_fwdcolor=#d59687
     regulatory      447..734
                     /label="CAG_enhancer"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
     primer          4193..4212
                     /label="SV40pro_F_primer"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     misc_feature    3519..3894
                     /label="5' Terminal Repeat"
                     /ApEinfo_revcolor=#d59687
                     /ApEinfo_fwdcolor=#d59687
     misc_feature    2849..2977
                     /label="destabilization"
                     /ApEinfo_revcolor=#9eafd2
                     /ApEinfo_fwdcolor=#9eafd2
ORIGIN
        1 CGTAAAATTG ACGCATGTGT TTTATCGATC TGTATATCGA GGTTTATTTA TTAATTTGAA
       61 TAGATATTAA GTTTTATTAT ATTTACACTT ACATACTAAT AATAAATTCA ACAAACAATT
      121 TATTTATGTT TATTTATTTA TTAAAAAAAA ACAAAAACTC AAAATTTCTT CTATAAAGTA
      181 ACAAAACTTT TAAACATTCT CTCTTTTACA AAAATAAACT TATTTTGTAC TTTAAAAACA
      241 GTCATGTTGT ATTATAAAAT AAGTAATTAG CTTAACTTAT ACATAATAGA AACAAATTAT
      301 ACTTATTAAT CGCATAACTT CGTATAATGT ATGCTATACG AAGTTATGCG ATTAAGGGAT
      361 CTGTAGGGCG CAGTAGTCCA ACTAGTTATT AATAGTAATC AATTACGGGG TCATTAGTTC
      421 ATAGCCCATA TATGGAGTTC CGCGTTACAT AACTTACGGT AAATGGCCCG CCTGGCTGAC
      481 CGCCCAACGA CCCCCGCCCA TTGACGTCAA TAATGACGTA TGTTCCCATA GTAACGCCAA
      541 TAGGGACTTT CCATTGACGT CAATGGGTGG ACTATTTACG GTAAACTGCC CACTTGGCAG
      601 TACATCAAGT GTATCATATG CCAAGTACGC CCCCTATTGA CGTCAATGAC GGTAAATGGC
      661 CCGCCTGGCA TTATGCCCAG TACATGACCT TATGGGACTT TCCTACTTGG CAGTACATCT
      721 ACGTATTAGT CATCGCTATT ACCATGGGTC GAGGTGAGCC CCACGTTCTG CTTCACTCTC
      781 CCCATCTCCC CCCCCTCCCC ACCCCCAATT TTGTATTTAT TTATTTTTTA ATTATTTTGT
      841 GCAGCGATGG GGGCGGGGGG GGGGGGGGCG CGCGCCAGGC GGGGCGGGGC GGGGCGAGGG
      901 GCGGGGCGGG GCGAGGCGGA GAGGTGCGGC GGCAGCCAAT CAGAGCGGCG CGCTCCGAAA
      961 GTTTCCTTTT ATGGCGAGGC GGCGGCGGCG GCGGCCCTAT AAAAAGCGAA GCGCGCGGCG
     1021 GGCGGGAGTC GCTGCGTTGC CTTCGCCCCG TGCCCCGCTC CGCGCCGCCT CGCGCCGCCC
     1081 GCCCCGGCTC TGACTGACCG CGTTACTCCC ACAGGTGAGC GGGCGGGACG GCCCTTCTCC
     1141 TCCGGGCTGT AATTAGCGCT TGGTTTAATG ACGGCTCGTT TCTTTTCTGT GGCTGCGTGA
     1201 AAGCCTTAAA GGGCTCCGGG AGGGCCCTTT GTGCGGGGGG GAGCGGCTCG GGGGGTGCGT
     1261 GCGTGTGTGT GTGCGTGGGG AGCGCCGCGT GCGGCCCGCG CTGCCCGGCG GCTGTGAGCG
     1321 CTGCGGGCGC GGCGCGGGGC TTTGTGCGCT CCGCGTGTGC GCGAGGGGAG CGCGGCCGGG
     1381 GGCGGTGCCC CGCGGTGCGG GGGGGCTGCG AGGGGAACAA AGGCTGCGTG CGGGGTGTGT
     1441 GCGTGGGGGG GTGAGCAGGG GGTGTGGGCG CGGCGGTCGG GCTGTAACCC CCCCCTGCAC
     1501 CCCCCTCCCC GAGTTGCTGA GCACGGCCCG GCTTCGGGTG CGGGGCTCCG TGCGGGGCGT
     1561 GGCGCGGGGC TCGCCGTGCC GGGCGGGGGG TGGCGGCAGG TGGGGGTGCC GGGCGGGGCG
     1621 GGGCCGCCTC GGGCCGGGGA GGGCTCGGGG GAGGGGCGCG GCGGCCCCGG AGCGCCGGCG
     1681 GCTGTCGAGG CGCGGCGAGC CGCAGCCATT GCCTTTTATG GTAATCGTGC GAGAGGGCGC
     1741 AGGGACTTCC TTTGTCCCAA ATCTGGCGGA GCCGAAATCT GGGAGGCGCC GCCGCACCCC
     1801 CTCTAGCGGG CGCGGGCGAA GCGGTGCGGC GCCGGCAGGA AGGAAATGGG CGGGGAGGGC
     1861 CTTCGTGCGT CGCCGCGCCG CCGTCCCCTT CTCCATCTCC AGCCTCGGGG CTGCCGCAGG
     1921 GGGACGGCTG CCTTCGGGGG GGACGGGGCA GGGCGGGGTT CGGCTTCTGG CGTGTGACCG
     1981 GCGGCTCTAG AGCCTCTGCT AACCATGTTC ATGCCTTCTT CTTTTTCCTA CAGCTCCTGG
     2041 GCAACGTGCT GGTTATTGTG CTGTCTCATC ATTTTGGCAA AGAATTCTGC AGTCGACGGT
     2101 ACCGCGGGCC CGGGATCCAC CGGTCGCCAC CATGGTGAGC AAGGGCGAGG AGCTGTTCAC
     2161 CGGGGTGGTG CCCATCCTGG TCGAGCTGGA CGGCGACGTA AACGGCCACA AGTTCAGCGT
     2221 GTCCGGCGAG GGCGAGGGCG ATGCCACCTA CGGCAAGCTG ACCCTGAAGT TCATCTGCAC
     2281 CACCGGCAAG CTGCCCGTGC CCTGGCCCAC CCTCGTGACC ACCCTGACCT ACGGCGTGCA
     2341 GTGCTTCAGC CGCTACCCCG ACCACATGAA GCAGCACGAC TTCTTCAAGT CCGCCATGCC
     2401 CGAAGGCTAC GTCCAGGAGC GCACCATCTT CTTCAAGGAC GACGGCAACT ACAAGACCCG
     2461 CGCCGAGGTG AAGTTCGAGG GCGACACCCT GGTGAACCGC ATCGAGCTGA AGGGCATCGA
     2521 CTTCAAGGAG GACGGCAACA TCCTGGGGCA CAAGCTGGAG TACAACTACA ACAGCCACAA
     2581 CGTCTATATC ATGGCCGACA AGCAGAAGAA CGGCATCAAG GTGAACTTCA AGATCCGCCA
     2641 CAACATCGAG GACGGCAGCG TGCAGCTCGC CGACCACTAC CAGCAGAACA CCCCCATCGG
     2701 CGACGGCCCC GTGCTGCTGC CCGACAACCA CTACCTGAGC ACCCAGTCCG CCCTGAGCAA
     2761 AGACCCCAAC GAGAAGCGCG ATCACATGGT CCTGCTGGAG TTCGTGACCG CCGCCGGGAT
     2821 CACTCTCGGC ATGGACGAGC TGTACAAGAA GCTTAGCCAT GGCTTCCCGC CGGAGGTGGA
     2881 GGAGCAGGAT GATGGCACGC TGCCCATGTC TTGTGCCCAG GAGAGCGGGA TGGACCGTCA
     2941 CCCTGCAGCC TGTGCTTCTG CTAGGATCAA TGTGTAGATG CGCGGCCGCA CTCCTCAGGT
     3001 GCAGGCTGCC TATCAGAAGG TGGTGGCTGG TGTGGCCAAT GCCCTGGCTC ACAAATACCA
     3061 CTGAGATCTT TTTCCCTCTG CCAAAAATTA TGGGGACATC ATGAAGCCCC TTGAGCATCT
     3121 GACTTCTGGC TAATAAAGGA AATTTATTTT CATTGCAATA GTGTGTTGGA ATTTTTTGTG
     3181 TCTCTCACTC GGAAGGACAT ATGGGAGGGC AAATCATTTA AAACATCAGA ATGAGTATTT
     3241 GGTTTAGAGT TTGGCAACAT ATGCCATATG CTGGCTGCCA TGAACAAAGG TGGCTATAAA
     3301 GAGGTCATCA GTATATGAAA CAGCCCCCTG CTGTCCATTC CTTATTCCAT AGAAAAGCCT
     3361 TGACTTGAGG TTAGATTTTT TTTATATTTT GTTTTGTGTT ATTTTTTTCT TTAACATCCC
     3421 TAAAATTTTC CTTACATGTT TTACTAGCCA GATTTTTCCT CCTCTCCTGA CTACTCCCAG
     3481 TCATAGCTGT CCCTCTTCTC TTATGAAGAT CCCTCGACCT GCAGGCTCCA GCCTACACAA
     3541 TCGCTCAAGA CGTGTAATGC TTTTATTATA TATTAGTCAC GATATCTATA ACAAGAAAAT
     3601 ATATATATAA TAAGTTATCA CGTAAGTAGA ACATGAAATA ACAATATAAT TATCGTATGA
     3661 GTTAAATCTT AAAAGTCACG TAAAAGATAA TCATGCGTCA TTTTGACTCA CGCGGTCGTT
     3721 ATAGTTCAAA ATCAGTGACA CTTACCGCAT TGACAAGCAC GCCTCACGGG AGCTCCAAGC
     3781 GGCGACTGAG ATGTCCTAAA TGCACAGCGA CGGATTCGCG CTATTTAGAA AGAGAGAGCA
     3841 ATATTTCAAG AATGCATGCG TCAATTTTAC GCAGACTATC TTTCTAGGGT TAAAAGCTTG
     3901 GCGTAATCAT GGTCATAGCT GTTTCCTGTG TGAAATTGTT ATCCGCTCAC AATTCCACAC
     3961 AACATACGAG CCGGAAGCAT AAAGTGTAAA GCCTGGGGTG CCTAATGAGT GAGCTAACTC
     4021 ACATTAATTG CGTTGCGCTC ACTGCCCGCT TTCCAGTCGG GAAACCTGTC GTGCCAGCGG
     4081 ATCCGCATCT CAATTAGTCA GCAACCATAG TCCCGCCCCT AACTCCGCCC ATCCCGCCCC
     4141 TAACTCCGCC CAGTTCCGCC CATTCTCCGC CCCATGGCTG ACTAATTTTT TTTATTTATG
     4201 CAGAGGCCGA GGCCGCCTCG GCCTCTGAGC TATTCCAGAA GTAGTGAGGA GGCTTTTTTG
     4261 GAGGCCTAGG CTTTTGCAAA AAGCTAACTT GTTTATTGCA GCTTATAATG GTTACAAATA
     4321 AAGCAATAGC ATCACAAATT TCACAAATAA AGCATTTTTT TCACTGCATT CTAGTTGTGG
     4381 TTTGTCCAAA CTCATCAATG TATCTTATCA TGTCTGGATC CGCTGCATTA ATGAATCGGC
     4441 CAACGCGCGG GGAGAGGCGG TTTGCGTATT GGGCGCTCTT CCGCTTCCTC GCTCACTGAC
     4501 TCGCTGCGCT CGGTCGTTCG GCTGCGGCGA GCGGTATCAG CTCACTCAAA GGCGGTAATA
     4561 CGGTTATCCA CAGAATCAGG GGATAACGCA GGAAAGAACA TGTGAGCAAA AGGCCAGCAA
     4621 AAGGCCAGGA ACCGTAAAAA GGCCGCGTTG CTGGCGTTTT TCCATAGGCT CCGCCCCCCT
     4681 GACGAGCATC ACAAAAATCG ACGCTCAAGT CAGAGGTGGC GAAACCCGAC AGGACTATAA
     4741 AGATACCAGG CGTTTCCCCC TGGAAGCTCC CTCGTGCGCT CTCCTGTTCC GACCCTGCCG
     4801 CTTACCGGAT ACCTGTCCGC CTTTCTCCCT TCGGGAAGCG TGGCGCTTTC TCAATGCTCA
     4861 CGCTGTAGGT ATCTCAGTTC GGTGTAGGTC GTTCGCTCCA AGCTGGGCTG TGTGCACGAA
     4921 CCCCCCGTTC AGCCCGACCG CTGCGCCTTA TCCGGTAACT ATCGTCTTGA GTCCAACCCG
     4981 GTAAGACACG ACTTATCGCC ACTGGCAGCA GCCACTGGTA ACAGGATTAG CAGAGCGAGG
     5041 TATGTAGGCG GTGCTACAGA GTTCTTGAAG TGGTGGCCTA ACTACGGCTA CACTAGAAGG
     5101 ACAGTATTTG GTATCTGCGC TCTGCTGAAG CCAGTTACCT TCGGAAAAAG AGTTGGTAGC
     5161 TCTTGATCCG GCAAACAAAC CACCGCTGGT AGCGGTGGTT TTTTTGTTTG CAAGCAGCAG
     5221 ATTACGCGCA GAAAAAAAGG ATCTCAAGAA GATCCTTTGA TCTTTTCTAC GGGGTCTGAC
     5281 GCTCAGTGGA ACGAAAACTC ACGTTAAGGG ATTTTGGTCA TGAGATTATC AAAAAGGATC
     5341 TTCACCTAGA TCCTTTTAAA TTAAAAATGA AGTTTTAAAT CAATCTAAAG TATATATGAG
     5401 TAAACTTGGT CTGACAGTTA CCAATGCTTA ATCAGTGAGG CACCTATCTC AGCGATCTGT
     5461 CTATTTCGTT CATCCATAGT TGCCTGACTC CCCGTCGTGT AGATAACTAC GATACGGGAG
     5521 GGCTTACCAT CTGGCCCCAG TGCTGCAATG ATACCGCGAG ACCCACGCTC ACCGGCTCCA
     5581 GATTTATCAG CAATAAACCA GCCAGCCGGA AGGGCCGAGC GCAGAAGTGG TCCTGCAACT
     5641 TTATCCGCCT CCATCCAGTC TATTAATTGT TGCCGGGAAG CTAGAGTAAG TAGTTCGCCA
     5701 GTTAATAGTT TGCGCAACGT TGTTGCCATT GCTACAGGCA TCGTGGTGTC ACGCTCGTCG
     5761 TTTGGTATGG CTTCATTCAG CTCCGGTTCC CAACGATCAA GGCGAGTTAC ATGATCCCCC
     5821 ATGTTGTGCA AAAAAGCGGT TAGCTCCTTC GGTCCTCCGA TCGTTGTCAG AAGTAAGTTG
     5881 GCCGCAGTGT TATCACTCAT GGTTATGGCA GCACTGCATA ATTCTCTTAC TGTCATGCCA
     5941 TCCGTAAGAT GCTTTTCTGT GACTGGTGAG TACTCAACCA AGTCATTCTG AGAATAGTGT
     6001 ATGCGGCGAC CGAGTTGCTC TTGCCCGGCG TCAATACGGG ATAATACCGC GCCACATAGC
     6061 AGAACTTTAA AAGTGCTCAT CATTGGAAAA CGTTCTTCGG GGCGAAAACT CTCAAGGATC
     6121 TTACCGCTGT TGAGATCCAG TTCGATGTAA CCCACTCGTG CACCCAACTG ATCTTCAGCA
     6181 TCTTTTACTT TCACCAGCGT TTCTGGGTGA GCAAAAACAG GAAGGCAAAA TGCCGCAAAA
     6241 AAGGGAATAA GGGCGACACG GAAATGTTGA ATACTCATAC TCTTCCTTTT TCAATATTAT
     6301 TGAAGCATTT ATCAGGGTTA TTGTCTCATG AGCGGATACA TATTTGAATG TATTTAGAAA
     6361 AATAAACAAA TAGGGGTTCC GCGCACATTT CCCCGAAAAG TGCCACCTGG GTCGACTTAA
     6421 CCCTAGAAAG ATAATCATAT TGTGACGTAC GTTAAAGATA ATCATG
//