LOCUS       Exported                3046 bp ds-DNA     circular SYN 13-AUG-2018
DEFINITION  .
ACCESSION   .
VERSION     .
KEYWORDS    pKEW270-pTet-empty
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3046)
  AUTHORS   Kyle Watters
  TITLE     Direct Submission
  JOURNAL   Exported Aug 13, 2018 from SnapGene 4.2.3
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..3046
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     misc_feature    40..666
                     /label=TetR
                     /note="ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 
                     0} width 5 offset 0
                     ApEinfo_revcolor=#ff7016
                     ApEinfo_fwdcolor=#ff7016"
                     /note="color: #ff7016"
     misc_feature    281..286
                     /label=XbaI-SpeI scar(1)
                     /note="ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 
                     0} width 5 offset 0
                     ApEinfo_revcolor=#ff00ff
                     ApEinfo_label=XbaI-SpeI scar
                     ApEinfo_fwdcolor=#ff00ff"
                     /note="color: #ff00ff"
     misc_feature    667..739
                     /label=tet promoter region
                     /note="ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 
                     0} width 5 offset 0
                     ApEinfo_revcolor=#ffff00
                     ApEinfo_fwdcolor=#ffff00"
                     /note="color: #ffff00"
     misc_feature    683..710
                     /label=-35 and -10 boxes for forward promoter (tet reg
                     /label=-35 and -10 boxes for forward promoter (tet 
                     regulated)
                     /note="ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 
                     0} width 5 offset 0
                     ApEinfo_revcolor=#7eff77
                     ApEinfo_fwdcolor=#7eff77"
                     /note="color: #7eff77"
     misc_feature    688..705
                     /label=Q1, Q2 promoter (tetR)
                     /note="ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 
                     0} width 5 offset 0
                     ApEinfo_revcolor=#ffff00
                     ApEinfo_fwdcolor=#ffff00"
                     /note="color: #ffff00"
     misc_feature    942..1031
                     /label=ECK120029600 terminator
                     /note="color: #a6acb3"
     terminator      1123..1150
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
                     /note="color: #ffffff"
     rep_origin      complement(1258..1940)
                     /direction=LEFT
                     /label=ColE1 origin
                     /note="ApEinfo_revcolor=gray50
                     ApEinfo_fwdcolor=gray50"
                     /note="color: #808080"
     misc_feature    1856..1861
                     /label=BamHI-BglII scar
                     /note="ApEinfo_revcolor=#f109ff
                     ApEinfo_fwdcolor=#f109ff"
                     /note="color: #f109ff"
     misc_feature    complement(1867..1872)
                     /label=BamHI-BglII scar(4)
                     /note="ApEinfo_label=BamHI-BglII scar
                     ApEinfo_revcolor=#f109ff
                     ApEinfo_fwdcolor=#f109ff"
                     /note="color: #f109ff; direction: LEFT"
     misc_feature    1953..1958
                     /label=BamHI-BglII scar(1)
                     /note="ApEinfo_label=BamHI-BglII scar
                     ApEinfo_revcolor=#f109ff
                     ApEinfo_fwdcolor=#f109ff"
                     /note="color: #f109ff"
     misc_feature    complement(1965..1970)
                     /label=BamHI-BglII scar(5)
                     /note="ApEinfo_label=BamHI-BglII scar
                     ApEinfo_revcolor=#f109ff
                     ApEinfo_fwdcolor=#f109ff"
                     /note="color: #f109ff; direction: LEFT"
     CDS             complement(2038..2697)
                     /label=AmpR
                     /note="ApEinfo_revcolor=yellow
                     ApEinfo_fwdcolor=yellow"
                     /note="color: #ffff00; direction: LEFT"
     misc_feature    2297..2302
                     /label=NheI-XbaI scar
                     /note="ApEinfo_revcolor=#f109ff
                     ApEinfo_fwdcolor=#f109ff"
                     /note="color: #f109ff"
     misc_feature    2733..2738
                     /label=BamHI-BglII scar(2)
                     /note="ApEinfo_label=BamHI-BglII scar
                     ApEinfo_revcolor=#f109ff
                     ApEinfo_fwdcolor=#f109ff"
                     /note="color: #f109ff"
     misc_feature    complement(2750..2755)
                     /label=BamHI-BglII scar(6)
                     /note="ApEinfo_label=BamHI-BglII scar
                     ApEinfo_revcolor=#f109ff
                     ApEinfo_fwdcolor=#f109ff"
                     /note="color: #f109ff; direction: LEFT"
     promoter        complement(2896..3000)
                     /gene="bla"
                     /label=AmpR promoter
                     /note="color: #ffffff; direction: LEFT"
ORIGIN
        1 gtggatatac cttactcgag ttagccttga taggacgtct taagacccac tttcacattt
       61 aagttgtttt tctaatccgc atatgatcaa ttcaaggccg aataagaagg ctggctctgc
      121 accttggtga tcaaataatt cgatagcttg tcgtaataat ggcggcatac tatcagtagt
      181 aggtgtttcc ctttcttctt tagcgacttg atgctcttga tcttccaata cgcaacctaa
      241 agtaaaatgc cccacagcgc tgagtgcata taatgcattc tctagtgaaa aaccttgttg
      301 gcataaaaag gctaattgat tttcgagagt ttcatactgt ttttctgtag gccgtgtacc
      361 taaatgtact tttgctccat cgcgatgact tagtaaagca catctaaaac ttttagcgtt
      421 attacgtaaa aaatcttgcc agctttcccc ttctaaaggg caaaagtgag tatggtgcct
      481 atctaacatc tcaatggcta aggcgtcgag caaagcccgc ttatttttta catgccaata
      541 caatgtaggc tgctctacac ctagcttctg ggcgagttta cgggttgtta aaccttcgat
      601 tccgacctca ttaagcagct ctaatgcgct gttaatcact ttacttttat ctaatctaga
      661 catcattaat tcctaatttt tgttgacact ctatcgttga tagagttatt ttaccactcc
      721 ctatcagtga tagagaaaag aattcaactg caggcactgc ccatggacct cggtaccgaa
      781 tagctagccg gtaatgcatt cgctagagct cctaaagcat gcgacctgca accggtctgt
      841 cacgtacgtc gccaccgtcg acgtcgttcg taagtagcct agataaataa aataatcagt
      901 taaccgcgag ccccatgcga gagtagggaa ctgccaggca tttcagccaa aaaacttaag
      961 accgccggtc ttgtccacta ccttgcagta atgcggtgga caggatcggc ggttttcttt
     1021 tctcttctca accgccggga gcggatttga acgttgcgaa gcaacggccc ggagggtggc
     1081 gggcaggacg cccgccataa actgccaggc atcaaattaa gcagaaggcc atcctgacgg
     1141 atggcctttt tgcgtttcta caaactctgc ggtaatacgg ttatccacag aatcagggga
     1201 taacgcagga aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc
     1261 cgcgttgctg gcgtttttcc acaggctccg cccccctgac gagcatcaca aaaatcgacg
     1321 ctcaagtcag aggtggcgaa acccgacagg actataaaga taccaggcgt ttccccctgg
     1381 aagctccctc gtgcgctctc ctgttccgac cctgccgctt accggatacc tgtccgcctt
     1441 tctcccttcg ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc tcagttcggt
     1501 gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg
     1561 cgccttatcc ggtaactatc gtcttgagtc caacccggta agacacgact tatcgccact
     1621 ggcagcagcc actggtaaca ggattagcag agcgaggtat gtaggcggtg ctacagagtt
     1681 cttgaagtgg tggcctaact acggctacac tagaaggaca gtatttggta tctgcgctct
     1741 gctgaagcca gttaccttcg gaaaaagagt tggtagctct tgatccggca aacaaaccac
     1801 cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc
     1861 tcaagaagat cctttgatct tttctacggg gtctgacgct cagtggaacg aaaactcacg
     1921 ttaagggatt ttggtcatga gattatcaaa aaggatcttc acctagatcc ttttaaatta
     1981 aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa acttggtctg acagttacca
     2041 atgcttaatc agtgaggcac ctatctcagc gatctgtcta tttcgttcat ccatagttgc
     2101 ctgactcccc gtcgtgtaga taactacgat acgggagggc ttaccatctg gccccagtgc
     2161 tgcaatgata ccgcgagacc cacgctcacc ggctccagat ttatcagcaa taaaccagcc
     2221 agccggaagg gccgagcgca gaagtggtcc tgcaacttta tccgcctcca tccagtctat
     2281 taattgttgc cgggaagcta gagtaagtag ttcgccagtt aatagtttgc gcaacgttgt
     2341 tgccattgct acaggcatcg tggtgtcacg ctcgtcgttt ggtatggctt cattcagctc
     2401 cggttcccaa cgatcaaggc gagttacatg atcccccatg ttgtgcaaaa aagcggttag
     2461 ctccttcggt cctccgatcg ttgtcagaag taagttggcc gcagtgttat cactcatggt
     2521 tatggcagca ctgcataatt ctcttactgt catgccatcc gtaagatgct tttctgtgac
     2581 tggtgagtac tcaaccaagt cattctgaga atagtgtatg cggcgaccga gttgctcttg
     2641 cccggcgtca atacgggata ataccgcgcc acatagcaga actttaaaag tgctcatcat
     2701 tggaaaacgt tcttcggggc gaaaactctc aaggatctta ccgctgttga gatccagttc
     2761 gatgtaaccc actcgtgcac ccaactgatc ttcagcatct tttactttca ccagcgtttc
     2821 tgggtgagca aaaacaggaa ggcaaaatgc cgcaaaaaag ggaataaggg cgacacggaa
     2881 atgttgaata ctcatactct tcctttttca atattattga agcatttatc agggttattg
     2941 tctcatgagc ggatacatat ttgaatgtat ttagaaaaat aaacaaatag gggttccgcg
     3001 cacatttccc cgaaaagtgc cacctgacgt ctaagaaacc attatt
//
