LOCUS       Exported                7552 bp ds-DNA     circular SYN 14-AUG-2018
DEFINITION  Ligation of inverted Fragment 2 into Fragment 2.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7552)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Aug 14, 2018 from SnapGene Viewer 4.2.3
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..7552
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     misc_feature    110..500
                     /label=dso
     misc_feature    579..2021
                     /label=rep
                     /note="rep"
     misc_feature    complement(2425..3107)
                     /label=RP4 oriT
                     /note="RP4 origin of single-stranded DNA transfer"
     misc_feature    2569..2940
                     /label=traJ
                     /note="traJ"
     misc_feature    complement(3371..4147)
                     /label=aac (3) IV
                     /note="apramycin resistance gene"
     misc_feature    complement(4407..4967)
                     /label=ori
                     /note="ori from pMB1"
     misc_feature    5384..5445
                     /label=P_ermE
                     /note="constitutive promoter"
     misc_feature    5715..7526
                     /label=gusA
                     /note="gusA"
ORIGIN
        1 ctagtatcgg acgtctatcg gcgcgtcagg agcgagcagg ggcaatgaac ggccacacca
       61 atgccaggat caacaggacc cagaaatggc acgagcccgg gattggcctc ccgggctcgc
      121 cgcccccgaa ccggcgcagc atgccgagtg aggacgtttg ctctgtgacg agcgtacgag
      181 agaacagcgc gtcgacacca ccgcgccccg ccggaccgaa gtgccactgc ggcaccccga
      241 tcgtccacat ccccgggaag cgccccaaaa tctactgctc ggacgcctgc cgaaagcgcg
      301 caaagcgtct cattgcggac cagtcacgca gcgtagcaag cacgactaca gcgggtaatc
      361 cggacgcggg ctttagtagt aagggaaaat ctccgtccac tgcaggtcag agcgctgtaa
      421 ctggaacggg ttctacccca aacgagggtg ccgcgacagc cgggcgggag gacccgcgcg
      481 acgagcggtt cgcccggcgg gaccggcacc agacggtgtc gctgaacgag gcgttcaagg
      541 gctgcgggac ccggctcact gccgggacgg ccgagctgat gtggaagccg ggcgaggcga
      601 cgtggggcaa cacgtgccgc tgcaacaacg tccacacctg cccgtggtgc atgagccgca
      661 tcctggccgt acggggctcg aacgtgcagc tcgccgccga cggtctcgcg gacgccgggt
      721 acgggctgca ccttggcacg aacacgttgc gccacttcga gcgcatggcg ttcggcaccg
      781 tccgcaaggg aatgcggcac ggcctggtcg cggtcttgca cgacggctgg aagggcgcgt
      841 acggctcgtc cggccgccgc tggcgcacga tgcgcgacga cttcgggatc atcggctacg
      901 agcgggcctt cgaggacacc ttcggctggg gctccggctg gcacctgcac tggcacacgc
      961 tctgggtcac ccgcgaggtg ctcggcccgg acgcccaggc cgcgttccgc gacgcactcg
     1021 ccggagcctg ggccgccggg gtcgaatccg cgggcggcta caccgtcagc gagacgtgcg
     1081 accggcccgg ttgctcctgt gagggcaagg gccacggcac cgacgtgcgc ccactcaacg
     1141 gcgcggacgc cgctgacggc gatgcgggca agcaggcccg ctacctctac aaggacggcg
     1201 acaagaccaa gggcggcgtc gccaagatcg gcctggagct ggccggacag aacttcaagg
     1261 ccggtcgcgg cgacgaccgc atgggaccgc tcgacctggg cgacgcggcg gccgccgagc
     1321 tgcagcggct tcgtcggccc ggcccgttcg tcgagaagta ccgcgagcgc gagttcggcg
     1381 tcttccaggt ccgcaagcac taccggtcgc agaacctgaa ccggctgatc aaggaactcg
     1441 gcatccagca ggacgtccgc accgaggagg agatcaccga cgacaccgag ggcctggtcg
     1501 ccatcgcggt catcccggcc tacatctggt accgatacat cgcccgcgtc gccggacgtc
     1561 gcctcgacct catcaaggtc gccgagacgt acggcctacc gggcgtccgg cggctcgtcg
     1621 agtcctgggg cctggtgtgg ggcaaggacg tcctggaccc gcccgccccg gaagcgccgg
     1681 ccgcgccggg cgaccttgac gctgaccaga tgaggttcga ggtcatgtcc gaggaggaag
     1741 ctgctttccg cgaggcgcgc cggaaggcca acgaggcccg cacggaggag ctggccgctt
     1801 cgctcgaccg agtgcgtcag ccgaagaagg aggcgatccg cccgacgatc agccttcgga
     1861 agcgcctgaa gccgaagccg gtcaccgtcg acgtcaagac tcccccgccc ggcgccgcgt
     1921 cgcccgtgtg ccgccgctgc aagggcaagc tcgccccggt actccagccc tggggccggc
     1981 tccccggaga ctgcctccgc gtcgacacag cggtcgcgta gggccgctga gggaccgcca
     2041 tgagaccgcc accccggaca tccgggtggc ggtctcatgg cggtcccggc gcgaaagccg
     2101 agggtctgcc tgccgtgagg tggccggcgg acctgggtta ctgctggaat cccgcgccca
     2161 gtccgagcgt ccgccggcgc ttttcacctg gctccaattg tacgtaatcg gatccatatg
     2221 acgtcgacgc gtctgcagaa gcttcgaatt cgagctcccg ggtaccatgg catgcctgca
     2281 ggtcgacgga tcttttccgc tgcataaccc tgcttcgggg tcattatagc gattttttcg
     2341 gtatatccat cctttttcgc acgatataca ggattttgcc aaagggttcg tgtagacttt
     2401 ccttggtgta tccaacggcg tcagccgggc aggataggtg aagtaggccc acccgcgagc
     2461 gggtgttcct tcttcactgt cccttattcg cacctggcgg tgctcaacgg gaatcctgct
     2521 ctgcgaggct ggccggctac cgccggcgta acagatgagg gcaagcggat ggctgatgaa
     2581 accaagccaa ccaggaaggg cagcccacct atcaaggtgt actgccttcc agacgaacga
     2641 agagcgattg aggaaaaggc ggcggcggcc ggcatgagcc tgtcggccta cctgctggcc
     2701 gtcggccagg gctacaaaat cacgggcgtc gtggactatg agcacgtccg cgagctggcc
     2761 cgcatcaatg gcgacctggg ccgcctgggc ggcctgctga aactctggct caccgacgac
     2821 ccgcgcacgg cgcggttcgg tgatgccacg atcctcgccc tgctggcgaa gatcgaagag
     2881 aagcaggacg agcttggcaa ggtcatgatg ggcgtggtcc gcccgagggc agagccatga
     2941 cttttttagc cgctaaaacg gccggggggt gcgcgtgatt gccaagcacg tccccatgcg
     3001 ctccatcaag aagagcgact tcgcggagct ggtgaagtac atcaccgacg agcaaggcaa
     3061 gaccgatccc cggggacctg caggtcgact ctagctggct ggccagggcc cgatacgtcg
     3121 cggtgagttc aggctttttc atatctcatt gcccccggac gagcgtctgc tccgccattc
     3181 gccgtccgcc gtgccaatcg gatcagccgt ccaaatgcgg gattttcgtt agtcggaggc
     3241 caaacggcat tgagcgtcag catatcatca gcgagctgaa gaaagacaat ccccgatccg
     3301 ctccacgtgt tgccccagca atcagcgcga ccttgcccct ccaacgtcat ctcgttctcc
     3361 gctcatgagc tcagccaatc gactggcgag cggcatcgca ttcttcgcat cccgcctctg
     3421 gcggatgcag gaagatcaac ggatctcggc ccagttgacc cagggctgtc gccacaatgt
     3481 cgcgggagcg gatcaaccga gcaaaggcat gaccgactgg accttccttc tgaaggctct
     3541 tctccttgag ccacctgtcc gccaaggcaa agcgctcaca gcagtggtca ttctcgagat
     3601 aatcgacgcg taccaacttg ccatcctgaa gaatggtgca gtgtctcggc accccatagg
     3661 gaacctttgc catcaactcg gcaagatgca gcgtcgtgtt ggcatcgtgt cccacgccga
     3721 ggagaagtac ctgcccatcg agttcatgga cacgggcgac cgggcttgca ggcgagtgag
     3781 gtggcagggg caatggatca gagatgatct gctctgcctg tggccccgct gccgcaaagg
     3841 caaatggatg ggcgctgcgc tttacatttg gcaggcgcca gaatgtgtca gagacaactc
     3901 caaggtccgg tgtaacgggc gacgtggcag gatcgaacgg ctcgtcgtcc agacctgacc
     3961 acgagggcat gacgagcgtc cctcccggac ccagcgcagc acgcagggcc tcgatcagtc
     4021 caagtggccc atcttcgagg ggccggacgc tacggaagga gctgtggacc agcagcacac
     4081 cgccgggggt aaccccaagg ttgagaagct gaccgatgag ctcggctttt cgccattcgt
     4141 attgcacgac attgcactcc accgctgatg acatcagtcg atcatagcac gatcaacggc
     4201 actgttgcaa atagtcggtg gtgataaact tatcatcccc ttttgctgat ggagctgcac
     4261 atgaaccaaa aggatctagg tgaagatcct ttttgataat ctcatgacca aaatccctta
     4321 acgtgagttt tcgttccact gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg
     4381 agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac cgctaccagc
     4441 ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa ctggcttcag
     4501 cagagcgcag ataccaaata ctgttcttct agtgtagccg tagttaggcc accacttcaa
     4561 gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag tggctgctgc
     4621 cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac cggataaggc
     4681 gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc gaacgaccta
     4741 caccgaactg agatacctac agcgtgagct atgagaaagc gccacgcttc ccgaagggag
     4801 aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca cgagggagct
     4861 tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc tctgacttga
     4921 gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc
     4981 ggccttttta cggttcctgg ccttttgctg gccttttgct cacatgttct ttcctgcgtt
     5041 atcccctgat tctgtggata accgtattac cgcctttgag tgagctgata ccgctcgccg
     5101 cagccgaacg accgagcgca gcgagtcagt gagcgaggaa gcggaagagc gcccaatacg
     5161 caaaccgcct ctccccgcgc gttggccgat tcattaatgc agctggcacg acaggtttcc
     5221 cgactggaaa gcgggcagtg agcgcaacgc aattaatgtg agttagctca ctcattaggc
     5281 accccaggct ttacacttta tgcttccggc tcgtatgttg tgtggaattg tgagcggata
     5341 acaatttcac acaggaaaca gctatgacat gattacgaat tgtaccgggc cccccctcga
     5401 ggtccagccc gacccgagca cgcgccggca cgcctggtcg atgtcggacc ggagttcgag
     5461 gtacgcggct tgcaggtcca ggaaggggac gtccatgcga gtgtccgttc gagtggcggc
     5521 ttgcgcccga tgctagtcgc ggttgatcgg cgatcgcagg tgcacgcggt cgatcttgac
     5581 ggctggcgag aggtgcgggg aggatctgac cgacgcggtc cacacgtggc accgcgatgc
     5641 tgttgtgggc acaatcgtgc cggttggtag gatcgacggt atcgataagc tagtcgagca
     5701 acggaggtac ggacatgctg cggcccgtcg aaaccccgac ccgcgagatc aagaagctgg
     5761 acggcctgtg ggcgttcagc ctcgaccggg agaactgcgg tatcgaccag cggtggtggg
     5821 agagcgccct ccaggagtcg cgcgccatcg ccgtccccgg cagcttcaac gaccagttcg
     5881 cggacgcgga catccgcaac tacgcgggga acgtctggta tcagcgggag gtgttcatcc
     5941 ccaagggctg ggccggtcag cgcatcgtgc tgcgcttcga cgccgtgacc cactacggca
     6001 aggtctgggt caacaaccag gaagtgatgg agcaccaggg cggctacacg cccttcgagg
     6061 cggacgtgac cccgtacgtc atcgccggca agtcggtccg catcacggtc tgcgtcaaca
     6121 acgagctcaa ctggcagacc atcccgcccg gcatggtgat cacggacgag aacggcaaga
     6181 agaagcagag ctacttccac gacttcttca actacgccgg catccaccgc tcggtcatgc
     6241 tgtacacgac gccgaacacc tgggtcgacg acatcacggt ggtcacccac gtggcccagg
     6301 actgcaacca cgcgtccgtg gactggcagg tcgtcgccaa cggcgacgtg agcgtggagc
     6361 tccgcgacgc cgagcagcag gtcgtggcga ccgggcaggg cacctcgggg accctccagg
     6421 tcgtgaaccc ccacctctgg cagccgggtg agggctacct gtacgagctg tgcgtgacgg
     6481 cgaagagcca gaccgagtgc gacatctacc ccctgcgcgt cggcatccgg tccgtggcgg
     6541 tcaagggcga gcagttcctg atcaaccaca agccgttcta cttcacgggc ttcggtcggc
     6601 acgaggacgc cgacctccgc ggcaagggct tcgacaacgt cctgatggtc cacgaccacg
     6661 ccctgatgga ctggatcggc gccaactcgt accggacctc gcactacccg tacgcggaag
     6721 agatgctgga ctgggcggac gagcacggga tcgtcgtcat cgacgaaacc gccgcggtgg
     6781 gcttcaacct gtccctcggc atcggcttcg aggccggcaa caagccgaaa gagctctact
     6841 ccgaagaggc cgtcaacggc gaaacccagc aggcccacct ccaggcgatc aaagagctca
     6901 tcgcccgcga caagaaccac ccgtccgtcg tgatgtggtc gatcgccaac gagccggaca
     6961 cccggccgca gggtgcccgg gagtacttcg ccccgctggc cgaggccacc cggaagctcg
     7021 accccacccg gcccatcacc tgcgtgaacg tgatgttctg cgacgcccac acggacacca
     7081 tctccgacct cttcgacgtc ctgtgcctga accgctacta cggctggtac gtgcagtccg
     7141 gggacctgga aacggccgag aaggtcctgg agaaagagct gctggcgtgg caggagaagc
     7201 tgcaccagcc gatcatcatc accgagtacg gcgtggacac gctggccggt ctgcactcca
     7261 tgtacaccga catgtggtcc gaagagtacc agtgcgcgtg gctggacatg taccaccgcg
     7321 tgttcgaccg cgtcagcgcg gtcgtcggcg agcaggtgtg gaacttcgcc gacttcgcca
     7381 cctcccaggg catcctccgc gtcgggggca acaagaaggg catcttcacc cgggaccgca
     7441 agcccaagtc ggcggcgttc ctgctccaga agcggtggac cgggatgaac ttcggggaga
     7501 agccgcagca gggcgggaag cagtgataag cattaccctg ttatccctag tt
//
