LOCUS       Exported                7396 bp ds-DNA     circular SYN 14-AUG-2018
DEFINITION  Ligation of Fragment 1 into Fragment 2.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7396)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Aug 14, 2018 from SnapGene Viewer 4.2.3
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..7396
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     misc_feature    117..417
                     /label=sso
                     /note="sso"
     misc_feature    470..718
                     /label=orit
     misc_feature    892..1695
                     /label=aac
     misc_feature    1951..2451
                     /label=ori
                     /note="ori"
     misc_feature    3074..3814
                     /label=strep-mcherry
     misc_feature    3823..3903
                     /label='ORF-MCS
     misc_feature    3864..4154
                     /label=fd
                     /note="fd"
     misc_feature    4386..5195
                     /label=tsr
     misc_feature    5758..7200
                     /label=rep
ORIGIN
        1 ggccgtcgac gacgacgcgt ccgcctgcct gcttttggcc ggcgggccgg gccgcggttg
       61 tccgccgggt cggaccggtc ccttggtcga ccgatgcctc ggccacgacg atgctgggcc
      121 cgaagcggta cgcgaacccg tgccgggctg cggcggatcg cggcagcagg agctccccgc
      181 ccgccacggc tggctggatg gtcgcctcac gcgcccgcag gggtgacggc gtgggtgcgg
      241 cggtcagccc cgtgatcggg cgtcagacgg ccgctgaggg accgcgacca cccctccctt
      301 cccgcctgcc gtctttcacc gcccggccgg tccgcagacg ccgtacggcc aggccacgga
      361 ggattacggg cagatggcgc ccacccggca cccgggctgg ggcaccctgg acagcacgaa
      421 ggcccgcccc actcaccggg gcgggccttc gcgttgagct agagttcccg ccagcctcgc
      481 agagcaggat tcccgttgag caccgccagg tgcgaataag ggacagtgaa gaaggaacac
      541 ccgctcgcgg gtgggcctac ttcacctatc ctgcccggct gacgccgttg gatacaccaa
      601 ggaaagtcta cacgaaccct ttggcaaaat cctgtatatc gtgcgaaaaa ggatggatat
      661 accgaaaaaa tcgctataat gaccccgaag cagggttatg cagcggaaaa tgcagctcac
      721 ggtaactgat gccgtatttg cagtaccagc gtacggccca cagaatgatg tcacgctgaa
      781 aatgccggcc tttgaatggg ttcatgtgca gctccatcag caaaagggga tgataagttt
      841 atcaccaccg actatttgca acagtgccgt tgatcgtgct atgatcgact gatgtcatca
      901 gcggtggagt gcaatgtcgt gcaatacgaa tggcgaaaag ccgagctcat cggtcagctt
      961 ctcaaccttg gggttacccc cggcggtgtg ctgctggtcc acagctcctt ccgtagcgtc
     1021 cggcccctcg aagatgggcc acttggactg atcgaggccc tgcgtgctgc gctgggtccg
     1081 ggagggacgc tcgtcatgcc ctcgtggtca ggtctggacg acgagccgtt cgatcctgcc
     1141 acgtcgcccg ttacaccgga ccttggagtt gtctctgaca cattctggcg cctgccaaat
     1201 gtaaagcgca gcgcccatcc atttgccttt gcggcagcgg ggccacaggc agagcagatc
     1261 atctctgatc cattgcccct gccacctcac tcgcctgcaa gcccggtcgc ccgtgtccat
     1321 gaactcgatg ggcaggtact tctcctcggc gtgggacacg atgccaacac gacgctgcat
     1381 cttgccgagt tgatggcaaa ggttccctat ggggtgccga gacactgcac cattcttcag
     1441 gatggcaagt tggtacgcgt cgattatctc gagaatgacc actgctgtga gcgctttgcc
     1501 ttggcggaca ggtggctcaa ggagaagagc cttcagaagg aaggtccagt cggtcatgcc
     1561 tttgctcggt tgatccgctc ccgcgacatt gtggcgacag ccctgggtca actgggccga
     1621 gatccgttga tcttcctgca tccgccagag gcgggatgcg aagaatgcga tgccgctcgc
     1681 cagtcgattg gctgagctca taagttccta ttccgaagtt cctattcgaa atgaccgacc
     1741 aagcgacgcc caacctgcca tcacgagatt tcgattccac cgccgccttc tatgaaaggt
     1801 tgggcttcgg aatcgttttc cgggacgccg gctggatgat cctccagcgc ggggatctca
     1861 tgctggagtt cttcgcccac cccaaaagga tctaggtgaa gatccttttt gataatctca
     1921 tgaccaaaat cccttaacgt gagttttcgt tccactgagc gtcagacccc gtagaaaaga
     1981 tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa
     2041 aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga
     2101 aggtaactgg cttcagcaga gcgcagatac caaatactgt ccttctagtg tagccgtagt
     2161 taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt
     2221 taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat
     2281 agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct
     2341 tggagcgaac gacctacacc gaactgagat acctacagcg tgagcattga gaaagcgcca
     2401 cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag
     2461 agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc
     2521 gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga
     2581 aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca
     2641 tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc tttgagtgag
     2701 ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg
     2761 aagagcgccc aatacgcaaa ccgcctctcc ccgcgcgttg gccgattcat taatgcagct
     2821 ggcacgacag gtttcccgac tggaaagcgg gcagtgagcg caacgcaatt aatgtgagtt
     2881 agctcactca ttaggcaccc caggctttac actttatgct tccggctcgt atgttgtgtg
     2941 gaattgtgag cggataacaa tttcacacag gaaacagcta tgaccatgat tacgaattgt
     3001 acgtagacct acgccttgac cttgatgagg cgcgtgagct acaatcaata ctcgattaga
     3061 agggaggtga catatgtgga gccacccgca gttcgagaag ggatccgtct ccaagggcga
     3121 ggaggacaac atggccatca tcaaggagtt catgcgcttc aaggtccaca tggagggctc
     3181 cgtcaacggc cacgagttcg agatcgaggg cgagggcgag ggccgcccgt acgagggcac
     3241 ccagaccgcc aagctgaagg tcaccaaggg cggcccgctg ccgttcgcct gggacatcct
     3301 gtccccgcag ttcatgtacg gctccaaggc ctacgtcaag cacccggccg acatcccgga
     3361 ctacctgaag ctgtccttcc cggagggctt caagtgggag cgcgtcatga acttcgagga
     3421 cggcggcgtc gtcaccgtca cccaggactc ctccctgcag gacggcgagt tcatctacaa
     3481 ggtcaagctg cgcggcacca acttcccgtc cgacggcccg gtcatgcaga agaagaccat
     3541 gggctgggag gcctcctccg agcgcatgta cccggaggac ggcgccctga agggcgagat
     3601 caagcagcgc ctgaagctga aggacggcgg ccactacgac gccgaggtca agaccaccta
     3661 caaggccaag aagccggtcc agctgccggg cgcctacaac gtcaacatca agctggacat
     3721 cacctcccac aacgaggact acaccatcgt cgagcagtac gagcgcgccg agggccgcca
     3781 ctccaccggc ggcatggacg agctgtacaa gtgaagctta gatccccggg taccgagctc
     3841 gaattcccca gatctaaagt tttgtcgtct ttccagacgt tagtaaatga attttctgta
     3901 tgaggttttg ctaaacaact ttcaacagtt tcagcggagt gagaatagaa aggaacaact
     3961 aaaggaattg cgaataataa ttttttcacg ttgaaaatct ccaaaaaaaa aggctccaaa
     4021 aggagccttt aattgtatcg gtttatcagc ttgctttcga ggtgaatttc ttaaacagct
     4081 tgataccgat agttgcgccg acaatgacaa caaccatcgc ccacgcataa ccgatatatt
     4141 cggtcgctga ggcttgcagg gagtcaaagg ccgcttttgc gggatctcgt cgaaggcact
     4201 agaaggccta tatgcgggga tcgaccgcgc gggtcccgga cggggaagag cggggagctt
     4261 tgccagagag cgacgacttc cccttgcgtt ggtgattgcc ggtcagggca gccatccgcc
     4321 atcgtcgcgt agggtgtcac accccaggaa tcgcgtcact gaacacagca gccggtagga
     4381 cgaccatgac tgagttggac accatcgcaa atccgtccga tcccgcggtg cagcggatca
     4441 tcgatgtcac caagccgtcg cgatccaaca taaagacaac gttgatcgag gacgtcgagc
     4501 ccctcatgca cagcatcgcg gccggggtgg agttcatcga ggtctacggc agcgacagca
     4561 gtccttttcc atctgagttg ctggatctgt gcgggcggca gaacataccg gtccgcctca
     4621 tcgactcctc gatcgtcaac cagttgttca agggggagcg gaaggccaag acattcggca
     4681 tcgcccgcgt ccctcgcccg gccaggttcg gcgatatcgc gagccggcgt ggggacgtcg
     4741 tcgttctcga cggggtgaag atcgtcggga acatcggcgc gatagtacgc acgtcgctcg
     4801 cgctcggagc gtcggggatc atcctggtcg acagtgacat caccagcatc gcggaccggc
     4861 gtctccaaag ggccagccga ggttacgtct tctcccttcc cgtcgttctc tccggtcgcg
     4921 aggaggccat cgccttcatt cgggacagcg gtatgcagct gatgacgctc aaggcggatg
     4981 gcgacatttc cgtgaaggaa ctcggggaca atccggatcg gctggccttg ctgttcggca
     5041 gcgaaaaggg tgggccttcc gacctgttcg aggaggcgtc ttccgcctcg gtttccatcc
     5101 ccatgatgag ccagaccgag tctctcaacg tttccgtttc cctcggaatc gcgctgcacg
     5161 agaggatcga caggaatctc gcggccaacc gataagcgcc tctgttcctc ggacgctcgg
     5221 ttcctcgacc tcgattcgtc agtgatgatc aacaggaccc agaaatggca cgagcccggg
     5281 attggcctcc cgggctcgcc gcccccgaac cggcgcagca tgccgagtga ggacgtttgc
     5341 tctgtgacga gcgtacgaga gaacagcgcg tcgacaccac cgcgccccgc cggaccgaag
     5401 tgccactgcg gcaccccgat cgtccacatc cccgggaagc gccccaaaat ctactgctcg
     5461 gacgcctgcc gaaagcgcgc aaagcgtctc attgcggacc agtcacgcag cgtagcaagc
     5521 acgactacag cgggtaatcc ggacgcgggc tttagtagta agggaaaatc tccgtccact
     5581 gcaggtcaga gcgctgtaac tggaacgggt tctaccccaa acgagggtgc cgcgacagcc
     5641 gggcgggagg acccgcgcga cgagcggttc gcccggcggg accggcacca gacggtgtcg
     5701 ctgaacgagg cgttcaaggg ctgcgggacc cggctcactg ccgggacggc cgagctgatg
     5761 tggaagccgg gcgaggcgac gtggggcaac acgtgccgct gcaacaacgt ccacacctgc
     5821 ccgtggtgca tgagccgcat cctggccgta cggggctcga acgtgcagct cgccgccgac
     5881 ggtctcgcgg acgccgggta cgggctgcac cttggcacga acacgttgcg ccacttcgag
     5941 cgcatggcgt tcggcaccgt ccgcaaggga atgcggcacg gcctggtcgc ggtcttgcac
     6001 gacggctgga agggcgcgta cggctcgtcc ggccgccgct ggcgcacgat gcgcgacgac
     6061 ttcgggatca tcggctacga gcgggccttc gaggacacct tcggctgggg ctccggctgg
     6121 cacctgcact ggcacacgct ctgggtcacc cgcgaggtgc tcggcccgga cgcccaggcc
     6181 gcgttccgcg acgcactcgc cggagcctgg gccgccgggg tcgaatccgc gggcggctac
     6241 accgtcagcg agacgtgcga ccggcccggt tgctcctgtg agggcaaggg ccacggcacc
     6301 gacgtgcgcc cactcaacgg cgcggacgcc gctgacggcg atgcgggcaa gcaggcccgc
     6361 tacctctaca aggacggcga caagaccaag ggcggcgtcg ccaagatcgg cctggagctg
     6421 gccggacaga acttcaaggc cggtcgcggc gacgaccgca tgggaccgct cgacctgggc
     6481 gacgcggcgg ccgccgagct gcagcggctt cgtcggcccg gcccgttcgt cgagaagtac
     6541 cgcgagcgcg agttcggcgt cttccaggtc cgcaagcact accggtcgca gaacctgaac
     6601 cggctgatca aggaactcgg catccagcag gacgtccgca ccgaggagga gatcaccgac
     6661 gacaccgagg gcctggtcgc catcgcggtc atcccggcct acatctggta ccgatacatc
     6721 gcccgcgtcg ccggacgtcg cctcgacctc atcaaggtcg ccgagacgta cggcctaccg
     6781 ggcgtccggc ggctcgtcga gtcctggggc ctggtgtggg gcaaggacgt cctggacccg
     6841 cccgccccgg aagcgccggc cgcgccgggc gaccttgacg ctgaccagat gaggttcgag
     6901 gtcatgtccg aggaggaagc tgctttccgc gaggcgcgcc ggaaggccaa cgaggcccgc
     6961 acggaggagc tggccgcttc gctcgaccga gtgcgtcagc cgaagaagga ggcgatccgc
     7021 ccgacgatca gccttcggaa gcgcctgaag ccgaagccgg tcaccgtcga cgtcaagact
     7081 cccccgcccg gcgccgcgtc gcccgtgtgc cgccgctgca agggcaagct cgccccggta
     7141 ctccagccct ggggccggct ccccggagac tgcctccgcg tcgacacagc ggtcgcgtag
     7201 ggccgctgag ggaccgccat gagaccgcca ccccggacat ccgggtggcg gtctcatggc
     7261 ggtcccggcg cgaaagccga gggtctgcct gccgtgaggt ggccggcgga cctgggttac
     7321 tgctggaatc ccgcgcccag tccgagcgtc cgccggcgct tttcacctgg ctccgggtgc
     7381 tcgtcgatga gcacca
//
