LOCUS Exported 8103 bp ds-DNA circular SYN 08-AUG-2018 DEFINITION synthetic circular DNA ACCESSION . VERSION . KEYWORDS . SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 8103) AUTHORS . TITLE Direct Submission JOURNAL Exported Aug 15, 2018 from SnapGene Viewer 4.2.3 http://www.snapgene.com FEATURES Location/Qualifiers source 1..8103 /organism="synthetic DNA construct" /mol_type="other DNA" CDS 68..1068 /locus_tag="Bra 5' HA (2 sil. muts.)" /label=Bra 5' HA (2 sil. muts.) misc_signal 1075..1140 /locus_tag="P2A" /label=P2A gene 1147..1857 /locus_tag="mCherry" /label=mCherry misc_recomb 1864..1897 /locus_tag="loxP" /label=loxP promoter 1915..2430 /locus_tag="PGK" CDS 2451..3047 /locus_tag="PuroR" /label=PuroR CDS 3054..3764 /locus_tag="sfGFP" /label=sfGFP misc_recomb 4035..4068 /locus_tag="loxP(1)" /label=loxP 3'UTR 4097..5076 /locus_tag="Bra 3' HA (Mod. PAM)" /label=Bra 3' HA (Mod. PAM) rep_origin complement(5247..5553) /direction=LEFT /locus_tag="F1 ori" /label=F1 ori promoter 5602..5701 /locus_tag="Bla promoter" /label=Bla promoter promoter 5864..6060 /locus_tag="SV40 early promoter" /label=SV40 early promoter CDS 6095..6886 /locus_tag="KanR" /label=KanR rep_origin join(7472..8103,1..41) /locus_tag="pUC ori" /label=pUC ori ORIGIN 1 tttttacggt tcctggcctt ttgctggcct tttgctcaca tgtgaattcg agacggtcga 61 cgctagcggg gttattggta ggtattacta attccacttt aacctgctct taaggcaaac 121 acacagatga caaacttctc aatggccctg aggactttgc ttaaggaccc aagtgatcgg 181 caggcagcca agtgctgatc actcgttcca atttggtgtc ttagtctgaa acagtaatct 241 gccttgattt acccaggctt gctctagagt ccgaatgcct ttgtagatgc cagagggttt 301 agcactcatt tcagcatcct cgttttatat cctgaaaaac agtcatgggg caaggtcaag 361 acgcagaact ggtcttcagc ccagctctgg ggtccctggc gtattgcccc atgctgacac 421 gctaatcccc tgtctactgg acaagcctgt gacagcaatg atggctttct aaagaatatg 481 cattctgtga atgcggggat tgtggggtgg ggttgggaac acattgccct gagccttggt 541 gctcagtgct aatcctgtat gccaatgagc acagagctac ttgtcagagt cctctttagg 601 aggactcggc acactaaaca gtcactgtgc ttatgggcac cttattggtt ttgtttgatg 661 cagtccatcc ctgtggtcat agtgaaaaaa aaaatagtct gcaaagccct gtgatgcaat 721 taagtactga ttcctccccc acatctcatc atagctcatt tgcaggcccc tgatgtctgt 781 tttctctatt tcagccagta tcccagtctc tggtctgtga gcaatggtac catcacccca 841 ggctcccaga cagctggggt gtccaacggg ctgggagctc agttctttcg aggctcccct 901 gcacattaca caccactgac acacacggtc tcagctgcca cgtcctcgtc ttctggttct 961 ccgatgtatg aaggggctgc tacagtcaca gacatttctg acagccagta tgacacggcc 1021 caaagcctcc tcatagcctc gtggacacct gtgtcacccc catctatgaa gcttggaagc 1081 ggagctacta acttcagcct gctgaagcag gctggagacg tggaggagaa ccctggacct 1141 gatatcatgg tgagcaaggg cgaggaggat aacatggcca tcatcaagga gttcatgcgc 1201 ttcaaggtgc acatggaggg ctccgtgaac ggccacgagt tcgagatcga gggcgagggc 1261 gagggccgcc cctacgaggg cacccagacc gccaagctga aggtgaccaa gggtggcccc 1321 ctgcccttcg cctgggacat cctgtcccct cagttcatgt acggctccaa ggcctacgtg 1381 aagcaccccg ccgacatccc cgactacttg aagctgtcct tccccgaggg cttcaagtgg 1441 gagcgcgtga tgaacttcga ggacggcggc gtggtgaccg tgacccagga ctcctccctg 1501 caggacggcg agttcatcta caaggtgaag ctgcgcggca ccaacttccc ctccgacggc 1561 cccgtaatgc agaagaagac catgggctgg gaggcctcct ccgagcggat gtaccccgag 1621 gacggcgccc tgaagggcga gatcaagcag aggctgaagc tgaaggacgg cggccactac 1681 gacgctgagg tcaagaccac ctacaaggcc aagaagcccg tgcagctgcc cggcgcctac 1741 aacgtcaaca tcaagttgga catcacctcc cacaacgagg actacaccat cgtggaacag 1801 tacgaacgcg ccgagggccg ccactccacc ggcggcatgg acgagctgta caagtaacgt 1861 acgataactt cgtatagcat acattatacg aagttatatc gatatatcga attcccacgg 1921 ggttggggtt gcgccttttc caaggcagcc ctgggtttgc gcagggacgc ggctgctctg 1981 ggcgtggttc cgggaaacgc agcggcgccg accctgggtc tcgcacattc ttcacgtccg 2041 ttcgcagcgt cacccggatc ttcgccgcta cccttgtggg ccccccggcg acgcttcctg 2101 ctccgcccct aagtcgggaa ggttccttgc ggttcgcggc gtgccggacg tgacaaacgg 2161 aagccgcacg tctcactagt accctcgcag acggacagcg ccagggagca atggcagcgc 2221 gccgaccgcg atgggctgtg gccaatagcg gctgctcagc agggcgcgcc gagagcagcg 2281 gccgggaagg ggcggtgcgg gaggcggggt gtggggcggt agtgtgggcc ctgttcctgc 2341 ccgcgcggtg ttccgcattc tgcaagcctc cggagcgcac gtcggcagtc ggctccctcg 2401 ttgaccgaat caccgacctc tctccccagg gggatccacc ggtcgccacc atgaccgagt 2461 acaagcccac ggtgcgcctc gccacccgcg acgacgtccc cagggccgta cgcaccctcg 2521 ccgccgcgtt cgccgactac cccgccacgc gccacaccgt cgatccggac cgccacatcg 2581 agcgggtcac cgagctgcaa gaactcttcc tcacgcgcgt cgggctcgac atcggcaagg 2641 tgtgggtcgc ggacgacggc gccgcggtgg cggtctggac cacgccggag agcgtcgaag 2701 cgggggcggt gttcgccgag atcggcccgc gcatggccga gttgagcggt tcccggctgg 2761 ccgcgcagca acagatggaa ggcctcctgg cgccgcaccg gcccaaggag cccgcgtggt 2821 tcctggccac cgtcggcgtc tcgcccgacc accagggcaa gggtctgggc agcgccgtcg 2881 tgctccccgg agtggaggcg gccgagcgcg ccggggtgcc cgccttcctg gagacctccg 2941 cgccccgcaa cctccccttc tacgagcggc tcggcttcac cgtcaccgcc gacgtcgagg 3001 tgcccgaagg accgcgcacc tggtgcatga cccgcaagcc cggtgccgat atctccaaag 3061 gagaagagct gttcactgga gtggtaccaa tacttgtgga gttggacgga gatgtgaacg 3121 gacacaaatt ttcagtccgc ggggaggggg aaggggatgc tactattggc aagctgacgc 3181 tcaaattcat ctgtaccacc ggaaaactcc ctgtaccctg gcccacactg gtgacaactc 3241 tgacttacgg cgtgcaatgt tttagccgat acccagacca catgaagagg cacgactttt 3301 tcaaaagcgc aatgcctgaa ggatacgtac aggaaaggac catttctttt aaagacgacg 3361 ggaagtacaa aacccgggca gtggtgaagt ttgagggcga taccctcgtc aataggatcg 3421 aattgaaggg aactgacttc aaagaagatg gcaacatcct gggtcacaag cttgagtata 3481 actttaactc ccacaacgtg tatattacag ccgacaaaca gaagaatgga attaaggcta 3541 acttcactgt cagacacaat gtcgaagatg gctccgtgca gctcgccgat cactatcaac 3601 agaatactcc tatcggggac ggcccagtcc tgctgcccga caaccactac ctgagtaccc 3661 agactgttct gagcaaagat ccgaacgaga agcgcgacca catggtgctg catgagtatg 3721 tcaacgctgc gggaattacc ctcggcatgg acgagctgta caagtaactc gagttccggt 3781 gaaacaggcg gccgcgactc tagatcataa tcagccatac cacatttgta gaggttttac 3841 ttgctttaaa aaacctccca cacctccccc tgaacctgaa acataaaatg aatgcaattg 3901 ttgttgttaa cttgtttatt gcagcttata atggttacaa ataaagcaat agcatcacaa 3961 atttcacaaa taaagcattt ttttcactgc attctagttg tggtttgtcc aaactcatca 4021 atgtatcttt taaaataact tcgtatagca tacattatac gaagttatta agctctagac 4081 tcaggtaccg catatgctga gacttgtaac aaccgctgtc aactggatct tctaggctca 4141 aagtggcagg ctcttgggac aagggaaaaa taaataaata aaagctagat actaacaact 4201 ccattttcaa ataagagcaa taatacatgt cctataatca tgttctacag cctcttgttt 4261 gatacctaca gtagtgatat gtgtcctaca ttatgaagcc aaggacagag agacggctgt 4321 ggtccagttt tttgtgactg gcagttaatc agagtccttt gctaggtagg gtcctatatc 4381 ttgtgtttct ctacaacata tatgtgactt tgaaatcctg gaattcgtcc accccctgtc 4441 ctactttagt gagacacaag gtacacctct aatgtcctcc cttgttgcct tagagtagtt 4501 aactttgagg acagaaaaaa gcatagccag aagattgtaa ctgaaccgtc aactgttctg 4561 cccttggaac atgcctactt taagcacacg tagctttttg tgttgggaag tcaactgtat 4621 ggatactttt ctgttgacaa agtagccaaa gacaatctgc agaaagtgtt ttctgcacaa 4681 taaaggcaat atatagcacc tggtctttgt ggattattct ctacacggct ccttttggga 4741 aaggctttct tccccagatg tatgttagtc agctgtgaac gttaatgttg tagccaaatc 4801 agttgcagaa ctgagggttt tcattttgta tacctatgca atctaaggct aaaaaacccc 4861 aaaaaacaaa gtgaaatgat gtaactttaa gtaacattga atcatagatg aatatgcatg 4921 gccaaactga aacctgctaa tgtcctaaag tccaggatag acgcctgata caagccactg 4981 atacataaat gagttgataa aagttggtta ctattaagtc tagttgccat aggtatctgt 5041 ctgcccatgg ctcttggact cattggctct tttccatcag tggggagatg ctgcagcata 5101 tgcttaaaag gcgtaaattg taagcgttaa tattttgtta aaattcgcgt taaatttttg 5161 ttaaatcagc tcatttttta accaataggc cgaaatcggc aaaatccctt ataaatcaaa 5221 agaatagacc gagatagggt tgagtgttgt tccagtttgg aacaagagtc cactattaaa 5281 gaacgtggac tccaacgtca aagggcgaaa aaccgtctat cagggcgatg gcccactacg 5341 tgaaccatca ccctaatcaa gttttttggg gtcgaggtgc cgtaaagcac taaatcggaa 5401 ccctaaaggg agcccccgat ttagagcttg acggggaaag ccggcgaacg tggcgagaaa 5461 ggaagggaag aaagcgaaag gagcgggcgc tagggcgctg gcaagtgtag cggtcacgct 5521 gcgcgtaacc accacacccg ccgcgcttaa tgcgccgcta cagggcgcgt caggtggcac 5581 ttttcgggga aatgtgcgcg gaacccctat ttgtttattt ttctaaatac attcaaatat 5641 gtatccgctc atgagacaat aaccctgata aatgcttcaa taatattgaa aaaggaagag 5701 tcctgaggcg gaaagaacca gctgtggaat gtgtgtcagt tagggtgtgg aaagtcccca 5761 ggctccccag caggcagaag tatgcaaagc atgcatctca attagtcagc aaccaggtgt 5821 ggaaagtccc caggctcccc agcaggcaga agtatgcaaa gcatgcatct caattagtca 5881 gcaaccatag tcccgcccct aactccgccc atcccgcccc taactccgcc cagttccgcc 5941 cattctccgc cccatggctg actaattttt tttatttatg cagaggccga ggccgcctcg 6001 gcctctgagc tattccagaa gtagtgagga ggcttttttg gaggcctagg cttttgcaaa 6061 gatcgatcaa gagacaggat gaggatcgtt tcgcatgatt gaacaagatg gattgcacgc 6121 aggttctccg gccgcttggg tggagaggct attcggctat gactgggcac aacagacaat 6181 cggctgctct gatgccgccg tgttccggct gtcagcgcag gggcgcccgg ttctttttgt 6241 caagaccgac ctgtccggtg ccctgaatga actgcaagac gaggcagcgc ggctatcgtg 6301 gctggccacg acgggcgttc cttgcgcagc tgtgctcgac gttgtcactg aagcgggaag 6361 ggactggctg ctattgggcg aagtgccggg gcaggatctc ctgtcatctc accttgctcc 6421 tgccgagaaa gtatccatca tggctgatgc aatgcggcgg ctgcatacgc ttgatccggc 6481 tacctgccca ttcgaccacc aagcgaaaca tcgcatcgag cgagcacgta ctcggatgga 6541 agccggtctt gtcgatcagg atgatctgga cgaagagcat caggggctcg cgccagccga 6601 actgttcgcc aggctcaagg cgagcatgcc cgacggcgag gatctcgtcg tgacccatgg 6661 cgatgcctgc ttgccgaata tcatggtgga aaatggccgc ttttctggat tcatcgactg 6721 tggccggctg ggtgtggcgg accgctatca ggacatagcg ttggctaccc gtgatattgc 6781 tgaagagctt ggcggcgaat gggctgaccg cttcctcgtg ctttacggta tcgccgctcc 6841 cgattcgcag cgcatcgcct tctatcgcct tcttgacgag ttcttctgag cgggactctg 6901 gggttcgaaa tgaccgacca agcgacgccc aacctgccat cacgagattt cgattccacc 6961 gccgccttct atgaaaggtt gggcttcgga atcgttttcc gggacgccgg ctggatgatc 7021 ctccagcgcg gggatctcat gctggagttc ttcgcccacc ctagggggag gctaactgaa 7081 acacggaagg agacaatacc ggaaggaacc cgcgctatga cggcaataaa aagacagaat 7141 aaaacgcacg gtgttgggtc gtttgttcat aaacgcgggg ttcggtccca gggctggcac 7201 tctgtcgata ccccaccgag accccattgg ggccaatacg cccgcgtttc ttccttttcc 7261 ccaccccacc ccccaagttc gggtgaaggc ccagggctcg cagccaacgt cggggcggca 7321 ggccctgcca tagcctcagg ttactcatat atactttaga ttgatttaaa acttcatttt 7381 taatttaaaa ggatctaggt gaagatcctt tttgataatc tcatgaccaa aatcccttaa 7441 cgtgagtttt cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga 7501 gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg 7561 gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc 7621 agagcgcaga taccaaatac tgtccttcta gtgtagccgt agttaggcca ccacttcaag 7681 aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc 7741 agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg 7801 cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac 7861 accgaactga gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga 7921 aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt 7981 ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag 8041 cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg 8101 gcc //