LOCUS Exported 8403 bp ds-DNA circular SYN 08-AUG-2018 DEFINITION synthetic circular DNA ACCESSION . VERSION . KEYWORDS . SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 8403) AUTHORS . TITLE Direct Submission JOURNAL Exported Aug 15, 2018 from SnapGene Viewer 4.2.3 http://www.snapgene.com FEATURES Location/Qualifiers source 1..8403 /organism="synthetic DNA construct" /mol_type="other DNA" promoter 6..416 /locus_tag="RSV/5'LTR hybrid promoter" /label=RSV/5'LTR hybrid promoter LTR complement(236..416) /locus_tag="LTR" misc_feature 524..569 /locus_tag="HIV-1 psi" /label=HIV-1 psi misc_feature 1060..1313 /locus_tag="HIV-1 RRE" /label=HIV-1 RRE misc_signal 1650..1689 /locus_tag="3' Splice acceptor" /label=3' Splice acceptor misc_feature 1799..1861 /locus_tag="central polyU tract" /label=central polyU tract promoter 1967..2482 /locus_tag="hPGK" CDS 2496..3041 /locus_tag="SNAP-tag" /label=SNAP-tag sig_peptide 3048..3284 /locus_tag="MD" /label=MD CDS 3291..4247 /locus_tag="Sox2 CDS" /label=Sox2 CDS misc_feature 4256..4848 /locus_tag="Woodchuck PRE" /label=Woodchuck PRE LTR complement(4985..5165) /locus_tag="LTR(1)" polyA_signal 5236..5389 /locus_tag="SV40 polyA" /label=SV40 polyA primer_bind complement(5544..5564) /locus_tag="T7" /label=T7 CDS 5661..5729 /locus_tag="LacZ alpha" /label=LacZ alpha rep_origin 5734..6189 /locus_tag="M13 origin" /label=M13 origin rep_origin 5751..6057 /locus_tag="F1 ori" /label=F1 ori promoter 6223..6322 /locus_tag="Bla promoter" /label=Bla promoter CDS 6323..7180 /locus_tag="AmpR" /label=AmpR rep_origin 7329..8001 /locus_tag="pUC ori" /label=pUC ori misc_binding 8300..8322 /locus_tag="LacO" /label=LacO primer_bind 8328..8348 /locus_tag="M13-rev" /label=M13-rev primer_bind 8366..8385 /locus_tag="T3" /label=T3 ORIGIN 1 agcttaatgt agtcttatgc aatactcttg tagtcttgca acatggtaac gatgagttag 61 caacatgcct tacaaggaga gaaaaagcac cgtgcatgcc gattggtgga agtaaggtgg 121 tacgatcgtg ccttattagg aaggcaacag acgggtctga catggattgg acgaaccact 181 gaattgccgc attgcagaga tattgtattt aagtgcctag ctcgatacaa taaacgggtc 241 tctctggtta gaccagatct gagcctggga gctctctggc taactaggga acccactgct 301 taagcctcaa taaagcttgc cttgagtgct tcaagtagtg tgtgcccgtc tgttgtgtga 361 ctctggtaac tagagatccc tcagaccctt ttagtcagtg tggaaaatct ctagcagtgg 421 cgcccgaaca gggacctgaa agcgaaaggg aaaccagagc tctctcgacg caggactcgg 481 cttgctgaag cgcgcacggc aagaggcgag gggcggcgac tggtgagtac gccaaaaatt 541 ttgactagcg gaggctagaa ggagagagat gggtgcgaga gcgtcagtat taagcggggg 601 agaattagat cgcgatggga aaaaattcgg ttaaggccag ggggaaagaa aaaatataaa 661 ttaaaacata tagtatgggc aagcagggag ctagaacgat tcgcagttaa tcctggcctg 721 ttagaaacat cagaaggctg tagacaaata ctgggacagc tacaaccatc ccttcagaca 781 ggatcagaag aacttagatc attatataat acagtagcaa ccctctattg tgtgcatcaa 841 aggatagaga taaaagacac caaggaagct ttagacaaga tagaggaaga gcaaaacaaa 901 agtaagacca ccgcacagca agcggccgct gatcttcaga cctggaggag gagatatgag 961 ggacaattgg agaagtgaat tatataaata taaagtagta aaaattgaac cattaggagt 1021 agcacccacc aaggcaaaga gaagagtggt gcagagagaa aaaagagcag tgggaatagg 1081 agctttgttc cttgggttct tgggagcagc aggaagcact atgggcgcag cctcaatgac 1141 gctgacggta caggccagac aattattgtc tggtatagtg cagcagcaga acaatttgct 1201 gagggctatt gaggcgcaac agcatctgtt gcaactcaca gtctggggca tcaagcagct 1261 ccaggcaaga atcctggctg tggaaagata cctaaaggat caacagctcc tggggatttg 1321 gggttgctct ggaaaactca tttgcaccac tgctgtgcct tggaatgcta gttggagtaa 1381 taaatctctg gaacagattt ggaatcacac gacctggatg gagtgggaca gagaaattaa 1441 caattacaca agcttaatac actccttaat tgaagaatcg caaaaccagc aagaaaagaa 1501 tgaacaagaa ttattggaat tagataaatg ggcaagtttg tggaattggt ttaacataac 1561 aaattggctg tggtatataa aattattcat aatgatagta ggaggcttgg taggtttaag 1621 aatagttttt gctgtacttt ctatagtgaa tagagttagg cagggatatt caccattatc 1681 gtttcagacc cacctcccaa ccccgagggg acccgacagg cccgaaggaa tagaagaaga 1741 aggtggagag agagacagag acagatccat tcgattagtg aacggatctc gacggtatcg 1801 gttaactttt aaaagaaaag gggggattgg ggggtacagt gcaggggaaa gaatagtaga 1861 cataatagca acagacatac aaactaaaga attacaaaaa caaattacaa aaattcaaaa 1921 ttttatcgat cacgagacta gcctcgagaa gcttgatatc gaattcccac ggggttgggg 1981 ttgcgccttt tccaaggcag ccctgggttt gcgcagggac gcggctgctc tgggcgtggt 2041 tccgggaaac gcagcggcgc cgaccctggg tctcgcacat tcttcacgtc cgttcgcagc 2101 gtcacccgga tcttcgccgc tacccttgtg ggccccccgg cgacgcttcc tgctccgccc 2161 ctaagtcggg aaggttcctt gcggttcgcg gcgtgccgga cgtgacaaac ggaagccgca 2221 cgtctcacta gtaccctcgc agacggacag cgccagggag caatggcagc gcgccgaccg 2281 cgatgggctg tggccaatag cggctgctca gcggggcgcg ccgagagcag cggccgggaa 2341 ggggcggtgc gggaggcggg gtgtggggcg gtagtgtggg ccctgttcct gcccgcgcgg 2401 tgttccgcat tctgcaagcc tccggagcgc acgtcggcag tcggctccct cgttgaccga 2461 atcaccgacc tctctcccca gggggatcca ccggtatgga caaagactgc gaaatgaagc 2521 gcaccaccct ggatagccct ctgggcaagc tggaactgtc tgggtgcgaa cagggcctgc 2581 accgtatcat cttcctgggc aaaggaacat ctgccgccga cgccgtggaa gtgcctgccc 2641 cagccgccgt gctgggcgga ccagagccac tgatgcaggc caccgcctgg ctcaacgcct 2701 actttcacca gcctgaggcc atcgaggagt tccctgtgcc agccctgcac cacccagtgt 2761 tccagcagga gagctttacc cgccaggtgc tgtggaaact gctgaaagtg gtgaagttcg 2821 gagaggtcat cagctacagc cacctggccg ccctggccgg caatcccgcc gccaccgccg 2881 ccgtgaaaac cgccctgagc ggaaatcccg tgcccattct gatcccctgc caccgggtgg 2941 tgcagggcga cctggacgtg gggggctacg agggcgggct cgccgtgaaa gagtggctgc 3001 tggcccacga gggccacaga ctgggcaagc ctgggctggg ttctagagca gaaaataagg 3061 ctaaagtctc aatggccggc gctaagcgcg ttcccgtgac cgtaactgcc gcttctaaac 3121 ccgggttgcg cccgaggact gctcttggag atattggcaa caaggtgtct gaagagctgc 3181 aggctcgcgt gccactgaag cgagaggcta agacgctggg aactggaaag ggtacggtta 3241 aggccctgcc gaagccagtg gagaaagttc ctgtctgcga accggctaga atgtataaca 3301 tgatggagac ggagctgaag ccgccgggcc cgcagcaagc ttcggggggc ggcggcggag 3361 gaggcaacgc cacggcggcg gcgaccggcg gcaaccagaa gaacagcccg gaccgcgtca 3421 agaggcccat gaacgccttc atggtatggt cccgggggca gcggcgtaag atggcccagg 3481 agaaccccaa gatgcacaac tcggagatca gcaagcgcct gggcgcggag tggaaacttt 3541 tgtccgagac cgagaagcgg ccgttcatcg acgaggccaa gcggctgcgc gctctgcaca 3601 tgaaggagca cccggattat aaataccggc cgcggcggaa aaccaagacg ctcatgaaga 3661 aggataagta cacgcttccc ggaggcttgc tggcccccgg cgggaacagc atggcgagcg 3721 gggttggggt gggcgccggc ctgggtgcgg gcgtgaacca gcgcatggac agctacgcgc 3781 acatgaacgg ctggagcaac ggcagctaca gcatgatgca ggagcagctg ggctacccgc 3841 agcacccggg cctcaacgct cacggcgcgg cacagatgca accgatgcac cgctacgacg 3901 tcagcgccct gcagtacaac tccatgacca gctcgcagac ctacatgaac ggctcgccca 3961 cctacagcat gtcctactcg cagcagggca cccccggtat ggcgctgggc tccatgggct 4021 ctgtggtcaa gtccgaggcc agctccagcc cccccgtggt tacctcttcc tcccactcca 4081 gggcgccctg ccaggccggg gacctccggg acatgatcag catgtacctc cccggcgccg 4141 aggtgccgga gcccgctgcg cccagtagac tgcacatggc ccagcactac cagagcggcc 4201 cggtgcccgg cacggccatt aacggcacac tgcccctgtc gcacatgtga gtcgacaatc 4261 aacctctgga ttacaaaatt tgtgaaagat tgactggtat tcttaactat gttgctcctt 4321 ttacgctatg tggatacgct gctttaatgc ctttgtatca tgctattgct tcccgtatgg 4381 ctttcatttt ctcctccttg tataaatcct ggttgctgtc tctttatgag gagttgtggc 4441 ccgttgtcag gcaacgtggc gtggtgtgca ctgtgtttgc tgacgcaacc cccactggtt 4501 ggggcattgc caccacctgt cagctccttt ccgggacttt cgctttcccc ctccctattg 4561 ccacggcgga actcatcgcc gcctgccttg cccgctgctg gacaggggct cggctgttgg 4621 gcactgacaa ttccgtggtg ttgtcgggga agctgacgtc ctttccatgg ctgctcgcct 4681 gtgttgccac ctggattctg cgcgggacgt ccttctgcta cgtcccttcg gccctcaatc 4741 cagcggacct tccttcccgc ggcctgctgc cggctctgcg gcctcttccg cgtcttcgcc 4801 ttcgccctca gacgagtcgg atctcccttt gggccgcctc cccgcctgga attcgagctc 4861 ggtaccttta agaccaatga cttacaaggc agctgtagat cttagccact ttttaaaaga 4921 aaagggggga ctggaagggc taattcactc ccaacgaaga caagatctgc tttttgcttg 4981 tactgggtct ctctggttag accagatctg agcctgggag ctctctggct aactagggaa 5041 cccactgctt aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct 5101 gttgtgtgac tctggtaact agagatccct cagacccttt tagtcagtgt ggaaaatctc 5161 tagcagtagt agttcatgtc atcttattat tcagtattta taacttgcaa agaaatgaat 5221 atcagagagt gagaggaact tgtttattgc agcttataat ggttacaaat aaagcaatag 5281 catcacaaat ttcacaaata aagcattttt ttcactgcat tctagttgtg gtttgtccaa 5341 actcatcaat gtatcttatc atgtctggct ctagctatcc cgcccctaac tccgcccagt 5401 tccgcccatt ctccgcccca tggctgacta atttttttta tttatgcaga ggccgaggcc 5461 gcctcggcct ctgagctatt ccagaagtag tgaggaggct tttttggagg cctaggcttt 5521 tgcgtcgaga cgtacccaat tcgccctata gtgagtcgta ttacgcgcgc tcactggccg 5581 tcgttttaca acgtcgtgac tgggaaaacc ctggcgttac ccaacttaat cgccttgcag 5641 cacatccccc tttcgccagc tggcgtaata gcgaagaggc ccgcaccgat cgcccttccc 5701 aacagttgcg cagcctgaat ggcgaatggc gcgacgcgcc ctgtagcggc gcattaagcg 5761 cggcgggtgt ggtggttacg cgcagcgtga ccgctacact tgccagcgcc ctagcgcccg 5821 ctcctttcgc tttcttccct tcctttctcg ccacgttcgc cggctttccc cgtcaagctc 5881 taaatcgggg gctcccttta gggttccgat ttagtgcttt acggcacctc gaccccaaaa 5941 aacttgatta gggtgatggt tcacgtagtg ggccatcgcc ctgatagacg gtttttcgcc 6001 ctttgacgtt ggagtccacg ttctttaata gtggactctt gttccaaact ggaacaacac 6061 tcaaccctat ctcggtctat tcttttgatt tataagggat tttgccgatt tcggcctatt 6121 ggttaaaaaa tgagctgatt taacaaaaat ttaacgcgaa ttttaacaaa atattaacgt 6181 ttacaatttc ccaggtggca cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt 6241 tttctaaata cattcaaata tgtatccgct catgagacaa taaccctgat aaatgcttca 6301 ataatattga aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc ttattccctt 6361 ttttgcggca ttttgccttc ctgtttttgc tcacccagaa acgctggtga aagtaaaaga 6421 tgctgaagat cagttgggtg cacgagtggg ttacatcgaa ctggatctca acagcggtaa 6481 gatccttgag agttttcgcc ccgaagaacg ttttccaatg atgagcactt ttaaagttct 6541 gctatgtggc gcggtattat cccgtattga cgccgggcaa gagcaactcg gtcgccgcat 6601 acactattct cagaatgact tggttgagta ctcaccagtc acagaaaagc atcttacgga 6661 tggcatgaca gtaagagaat tatgcagtgc tgccataacc atgagtgata acactgcggc 6721 caacttactt ctgacaacga tcggaggacc gaaggagcta accgcttttt tgcacaacat 6781 gggggatcat gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa 6841 cgacgagcgt gacaccacga tgcctgtagc aatggcaaca acgttgcgca aactattaac 6901 tggcgaacta cttactctag cttcccggca acaattaata gactggatgg aggcggataa 6961 agttgcagga ccacttctgc gctcggccct tccggctggc tggtttattg ctgataaatc 7021 tggagccggt gagcgtgggt ctcgcggtat cattgcagca ctggggccag atggtaagcc 7081 ctcccgtatc gtagttatct acacgacggg gagtcaggca actatggatg aacgaaatag 7141 acagatcgct gagataggtg cctcactgat taagcattgg taactgtcag accaagttta 7201 ctcatatata ctttagattg atttaaaact tcatttttaa tttaaaagga tctaggtgaa 7261 gatccttttt gataatctca tgaccaaaat cccttaacgt gagttttcgt tccactgagc 7321 gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat 7381 ctgctgcttg caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga 7441 gctaccaact ctttttccga aggtaactgg cttcagcaga gcgcagatac caaatactgt 7501 ccttctagtg tagccgtagt taggccacca cttcaagaac tctgtagcac cgcctacata 7561 cctcgctctg ctaatcctgt taccagtggc tgctgccagt ggcgataagt cgtgtcttac 7621 cgggttggac tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg 7681 ttcgtgcaca cagcccagct tggagcgaac gacctacacc gaactgagat acctacagcg 7741 tgagctatga gaaagcgcca cgcttcccga agggagaaag gcggacaggt atccggtaag 7801 cggcagggtc ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct 7861 ttatagtcct gtcgggtttc gccacctctg acttgagcgt cgatttttgt gatgctcgtc 7921 aggggggcgg agcctatgga aaaacgccag caacgcggcc tttttacggt tcctggcctt 7981 ttgctggcct tttgctcaca tgttctttcc tgcgttatcc cctgattctg tggataaccg 8041 tattaccgcc tttgagtgag ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga 8101 gtcagtgagc gaggaagcgg aagagcgccc aatacgcaaa ccgcctctcc ccgcgcgttg 8161 gccgattcat taatgcagct ggcacgacag gtttcccgac tggaaagcgg gcagtgagcg 8221 caacgcaatt aatgtgagtt agctcactca ttaggcaccc caggctttac actttatgct 8281 tccggctcgt atgttgtgtg gaattgtgag cggataacaa tttcacacag gaaacagcta 8341 tgaccatgat tacgccaagc gcgcaattaa ccctcactaa agggaacaaa agctggagct 8401 gca //