LOCUS       pTW081_Lvl0_-98/        2811 bp    DNA     circular     28-JAN-2014
DEFINITION  Cloning vector pUC19, complete sequence.
ACCESSION   urn.local...hs-9e4hqca
KEYWORDS    .
SOURCE      Cloning vector pUC19
  ORGANISM  Cloning vector pUC19
            other sequences; artificial sequences; vectors.
REFERENCE   1  (bases 1 to 2686)
  AUTHORS   New, England and Biolabs.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-OCT-2007) Research Department, New England Biolabs,
            240 County Road, Ipswich, MA 01938, USA COMMENT See also GenBank
            accession L09137.
FEATURES             Location/Qualifiers
     source          1..2811
                     /organism="Cloning vector pUC19"
                     /mol_type="other DNA"
     gene            55..655
                     /standard_name="lacZ PCR Product from pUC19"
     primer_bind     55..75
                     /standard_name="TK_DI_lacZ_Fwd_1"
     CDS             complement(146..469)
                     /gene="lacZalpha"
                     /codon_start=1
                     /product="beta-galactosidase alpha fragment"
                     /translation="MTMITPSLHACRSTLEDPRVPSSNSLAVVLQRRDWENPGVTQLN
                     RLAAHPPFASWRNSEEARTDRPSQQLRSLNGEWRLMRYFLLTHLCGISHRIWCTLSTI
                     CSDAA"
     gene            complement(146..469)
                     /gene="lacZalpha"
     primer_bind     364..386
                     /standard_name="M13/pUC Forward (Quintara)"
     primer_bind     378..395
                     /standard_name="M13 (-21) Forward (Quintara)"
     misc_feature    396..452
                     /gene="lacZalpha"
                     /note="multiple cloning site (EcoRI-HindIII)"
                     /note="Geneious type: polylinker"
     primer_bind     complement(465..481)
                     /standard_name="M13 Reverse (Quintara)"
     -10_signal      complement(514..519)
                     /note="Plac promoter (drives lacZalpha;
                     counter-clockwise); TATGTT"
     -35_signal      complement(538..543)
                     /note="Plac promoter (drives lacZalpha;
                     counter-clockwise); TTTACA"
     protein_bind    complement(563..575)
                     /note="CAP protein binding site"
                     /bound_moiety="catabolite activator protein (CAP)"
     primer_bind     578..598
                     /standard_name="TW_lvl0_seq_Fwd1"
     misc_feature    679..693
                     /standard_name="15 bp SapI Overhang"
     misc_feature    694..699
                     /standard_name="BsaI"
     primer_bind     694..815
                     /standard_name="TW_lvl0_98-3_NA_Fwd"
     primer_bind     complement(694..815)
                     /standard_name="TW_lvl0_98-3_NA_Rev"
     misc_feature    701..704
                     /standard_name="BsaI Cut Site"
     misc_feature    702..>802
                     /standard_name="98/5 SGP"
     primer_bind     719..740
                     /standard_name="TK-VEE-nsP4-seq-F"
     primer_bind     755..769
                     /standard_name="ApaI 15 bp Overhang"
     misc_feature    800..>802
                     /standard_name="Subgenomic RNA 5'UTR"
     misc_feature    802^803
                     /note="Geneious type: Editing History Deletion"
                     /standard_name="GACTACGACATAGTCTAGTCCGCCAAG"
     misc_feature    complement(805..808)
                     /standard_name="BsaI Cut Site"
     misc_feature    complement(810..815)
                     /standard_name="BsaI"
     primer_bind     complement(901..921)
                     /standard_name="TW_lvl0_seq_Rev1"
     rep_origin      complement(933..1606)
     misc_RNA        complement(992..1544)
                     /note="RNAII transcript (complementary strand)"
     rep_origin      complement(992..1580)
     rep_origin      complement(992..1525)
     primer_bind     complement(1031..1051)
                     /standard_name="TW_HDV-seq-R"
     primer_bind     1032..1053
                     /standard_name="pHAGE-A7550-7050"
     primer_bind     complement(1296..1320)
                     /standard_name="pHAGE-S7501-8000"
     primer_bind     complement(1340..1359)
                     /standard_name="RepO-R"
     -35_signal      1398..1403
                     /note="RNAI promoter (clockwise); TTGAAG"
     -10_signal      1420..1425
                     /note="RNAI promoter (clockwise); GCTACA"
     misc_RNA        1434..1541
                     /note="RNAI transcript"
     primer_bind     1543..1564
                     /standard_name="pHAGE-A7050-6550"
     -10_signal      complement(1554..1559)
                     /note="RNAII promoter (counter-clockwise); CGTAAT"
     -35_signal      complement(1575..1580)
                     /note="RNAII promoter (counter-clockwise); TTGAGA"
     CDS             complement(1751..2611)
                     /gene="bla"
                     /note="ampR (confers resistance to ampicillin)"
                     /codon_start=1
                     /product="beta-lactamase"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE
                     YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
                     DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
                     LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
                     EIGASLIKHW"
     gene            complement(1751..2611)
                     /gene="bla"
     primer_bind     complement(1800..1824)
                     /standard_name="pHAGE-S7001-7500"
     misc_feature    complement(1891..1896)
                     /standard_name="BsaI site"
     primer_bind     2052..2075
                     /standard_name="pHAGE-A6550-6050"
     primer_bind     complement(2320..2344)
                     /standard_name="pHAGE-S6501-7000"
     primer_bind     2374..2393
                     /standard_name="Amp-R; Quintara AmpR"
     primer_bind     2521..2544
                     /standard_name="pHAGE-A6050-5550"
     sig_peptide     complement(2543..2611)
                     /gene="bla"
                     /note="required for secretion to the periplasm; cleaved
                     off to form the mature beta-lactamase protein."
     promoter        complement(2612..2646)
                     /note="Beta Lactamase Promoter"
                     /standard_name="P-Bla"
     -10_signal      complement(2655..2660)
                     /note="bla promoter (counter-clockwise); GAGACA"
     -35_signal      complement(2676..2681)
                     /note="bla promoter (counter-clockwise); TTCAAA"
ORIGIN      
        1 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca
       61 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg
      121 ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc
      181 accatatgcg gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc atcaggcgcc
      241 attcgccatt caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcttcgctat
      301 tacgccagct ggcgaaaggg ggatgtgctg caaggcgatt aagttgggta acgccagggt
      361 tttcccagtc acgacgttgt aaaacgacgg ccagtgaatt cgagctcggt acccggggat
      421 cctctagagt cgacctgcag gcatgcaagc ttggcgtaat catggtcata gctgtttcct
      481 gtgtgaaatt gttatccgct cacaattcca cacaacatac gagccggaag cataaagtgt
      541 aaagcctggg gtgcctaatg agtgagctaa ctcacattaa ttgcgttgcg ctcactgccc
      601 gctttccagt cgggaaacct gtcgtgccag ctgcattaat gaatcggcca acgcgcgggg
      661 agaggcggtt tgcgtattgg gcgctcttcc gctggtctcc gacttccatc atagttatgg
      721 ccatgactac tctagctagc agtgttaaat cattcagcta cctgagaggg gcccctataa
      781 ctctctacgg ctaacctgaa tggccacctg agaccgcttc ctcgctcact gactcgctgc
      841 gctcggtcgt tcggctgcgg cgagcggtat cagctcactc aaaggcggta atacggttat
      901 ccacagaatc aggggataac gcaggaaaga acatgtgagc aaaaggccag caaaaggcca
      961 ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag gctccgcccc cctgacgagc
     1021 atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc gacaggacta taaagatacc
     1081 aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg
     1141 gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta
     1201 ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg
     1261 ttcagcccga ccgctgcgcc ttatccggta actatcgtct tgagtccaac ccggtaagac
     1321 acgacttatc gccactggca gcagccactg gtaacaggat tagcagagcg aggtatgtag
     1381 gcggtgctac agagttcttg aagtggtggc ctaactacgg ctacactaga agaacagtat
     1441 ttggtatctg cgctctgctg aagccagtta ccttcggaaa aagagttggt agctcttgat
     1501 ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag cagattacgc
     1561 gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc tacggggtct gacgctcagt
     1621 ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt atcaaaaagg atcttcacct
     1681 agatcctttt aaattaaaaa tgaagtttta aatcaatcta aagtatatat gagtaaactt
     1741 ggtctgacag ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc tgtctatttc
     1801 gttcatccat agttgcctga ctccccgtcg tgtagataac tacgatacgg gagggcttac
     1861 catctggccc cagtgctgca atgataccgc gagacccacg ctcaccggct ccagatttat
     1921 cagcaataaa ccagccagcc ggaagggccg agcgcagaag tggtcctgca actttatccg
     1981 cctccatcca gtctattaat tgttgccggg aagctagagt aagtagttcg ccagttaata
     2041 gtttgcgcaa cgttgttgcc attgctacag gcatcgtggt gtcacgctcg tcgtttggta
     2101 tggcttcatt cagctccggt tcccaacgat caaggcgagt tacatgatcc cccatgttgt
     2161 gcaaaaaagc ggttagctcc ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag
     2221 tgttatcact catggttatg gcagcactgc ataattctct tactgtcatg ccatccgtaa
     2281 gatgcttttc tgtgactggt gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc
     2341 gaccgagttg ctcttgcccg gcgtcaatac gggataatac cgcgccacat agcagaactt
     2401 taaaagtgct catcattgga aaacgttctt cggggcgaaa actctcaagg atcttaccgc
     2461 tgttgagatc cagttcgatg taacccactc gtgcacccaa ctgatcttca gcatctttta
     2521 ctttcaccag cgtttctggg tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa
     2581 taagggcgac acggaaatgt tgaatactca tactcttcct ttttcaatat tattgaagca
     2641 tttatcaggg ttattgtctc atgagcggat acatatttga atgtatttag aaaaataaac
     2701 aaataggggt tccgcgcaca tttccccgaa aagtgccacc tgacgtctaa gaaaccatta
     2761 ttatcatgac attaacctat aaaaataggc gtatcacgag gccctttcgt c
//
