LOCUS       pTW082_Lvl0_-98/        2813 bp    DNA     circular     28-JAN-2014
DEFINITION  Cloning vector pUC19, complete sequence.
ACCESSION   urn.local...ht-9e4hqca
KEYWORDS    .
SOURCE      Cloning vector pUC19
  ORGANISM  Cloning vector pUC19
            other sequences; artificial sequences; vectors.
REFERENCE   1  (bases 1 to 2686)
  AUTHORS   New, England and Biolabs.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-OCT-2007) Research Department, New England Biolabs,
            240 County Road, Ipswich, MA 01938, USA COMMENT See also GenBank
            accession L09137.
FEATURES             Location/Qualifiers
     source          1..2813
                     /organism="Cloning vector pUC19"
                     /mol_type="other DNA"
     gene            55..655
                     /standard_name="lacZ PCR Product from pUC19"
     primer_bind     55..75
                     /standard_name="TK_DI_lacZ_Fwd_1"
     CDS             complement(146..469)
                     /gene="lacZalpha"
                     /codon_start=1
                     /product="beta-galactosidase alpha fragment"
                     /translation="MTMITPSLHACRSTLEDPRVPSSNSLAVVLQRRDWENPGVTQLN
                     RLAAHPPFASWRNSEEARTDRPSQQLRSLNGEWRLMRYFLLTHLCGISHRIWCTLSTI
                     CSDAA"
     gene            complement(146..469)
                     /gene="lacZalpha"
     primer_bind     364..386
                     /standard_name="M13/pUC Forward (Quintara)"
     primer_bind     378..395
                     /standard_name="M13 (-21) Forward (Quintara)"
     misc_feature    396..452
                     /gene="lacZalpha"
                     /note="multiple cloning site (EcoRI-HindIII)"
                     /note="Geneious type: polylinker"
     primer_bind     complement(465..481)
                     /standard_name="M13 Reverse (Quintara)"
     -10_signal      complement(514..519)
                     /note="Plac promoter (drives lacZalpha;
                     counter-clockwise); TATGTT"
     -35_signal      complement(538..543)
                     /note="Plac promoter (drives lacZalpha;
                     counter-clockwise); TTTACA"
     protein_bind    complement(563..575)
                     /note="CAP protein binding site"
                     /bound_moiety="catabolite activator protein (CAP)"
     primer_bind     578..598
                     /standard_name="TW_lvl0_seq_Fwd1"
     misc_feature    679..693
                     /standard_name="15 bp SapI Overhang"
     misc_feature    694..699
                     /standard_name="BsaI"
     primer_bind     694..817
                     /standard_name="TW_lvl0_98-5_NA_Fwd"
     primer_bind     complement(694..817)
                     /standard_name="TW_lvl0_98-5_NA_Rev"
     misc_feature    701..704
                     /standard_name="BsaI Cut Site"
     misc_feature    702..804
                     /standard_name="98/5 SGP"
     primer_bind     719..740
                     /standard_name="TK-VEE-nsP4-seq-F"
     primer_bind     755..769
                     /standard_name="ApaI 15 bp Overhang"
     misc_feature    800..>804
                     /standard_name="Subgenomic RNA 5'UTR"
     misc_feature    804^805
                     /note="Geneious type: Editing History Deletion"
                     /standard_name="CTACGACATAGTCTAGTCCGCCAAG"
     misc_feature    complement(807..810)
                     /standard_name="BsaI Cut Site"
     misc_feature    complement(812..817)
                     /standard_name="BsaI"
     primer_bind     complement(903..923)
                     /standard_name="TW_lvl0_seq_Rev1"
     rep_origin      complement(935..1608)
     misc_RNA        complement(994..1546)
                     /note="RNAII transcript (complementary strand)"
     rep_origin      complement(994..1582)
     rep_origin      complement(994..1527)
     primer_bind     complement(1033..1053)
                     /standard_name="TW_HDV-seq-R"
     primer_bind     1034..1055
                     /standard_name="pHAGE-A7550-7050"
     primer_bind     complement(1298..1322)
                     /standard_name="pHAGE-S7501-8000"
     primer_bind     complement(1342..1361)
                     /standard_name="RepO-R"
     -35_signal      1400..1405
                     /note="RNAI promoter (clockwise); TTGAAG"
     -10_signal      1422..1427
                     /note="RNAI promoter (clockwise); GCTACA"
     misc_RNA        1436..1543
                     /note="RNAI transcript"
     primer_bind     1545..1566
                     /standard_name="pHAGE-A7050-6550"
     -10_signal      complement(1556..1561)
                     /note="RNAII promoter (counter-clockwise); CGTAAT"
     -35_signal      complement(1577..1582)
                     /note="RNAII promoter (counter-clockwise); TTGAGA"
     CDS             complement(1753..2613)
                     /gene="bla"
                     /note="ampR (confers resistance to ampicillin)"
                     /codon_start=1
                     /product="beta-lactamase"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE
                     YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
                     DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
                     LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
                     EIGASLIKHW"
     gene            complement(1753..2613)
                     /gene="bla"
     primer_bind     complement(1802..1826)
                     /standard_name="pHAGE-S7001-7500"
     misc_feature    complement(1893..1898)
                     /standard_name="BsaI site"
     primer_bind     2054..2077
                     /standard_name="pHAGE-A6550-6050"
     primer_bind     complement(2322..2346)
                     /standard_name="pHAGE-S6501-7000"
     primer_bind     2376..2395
                     /standard_name="Amp-R; Quintara AmpR"
     primer_bind     2523..2546
                     /standard_name="pHAGE-A6050-5550"
     sig_peptide     complement(2545..2613)
                     /gene="bla"
                     /note="required for secretion to the periplasm; cleaved
                     off to form the mature beta-lactamase protein."
     promoter        complement(2614..2648)
                     /note="Beta Lactamase Promoter"
                     /standard_name="P-Bla"
     -10_signal      complement(2657..2662)
                     /note="bla promoter (counter-clockwise); GAGACA"
     -35_signal      complement(2678..2683)
                     /note="bla promoter (counter-clockwise); TTCAAA"
ORIGIN      
        1 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca
       61 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg
      121 ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc
      181 accatatgcg gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc atcaggcgcc
      241 attcgccatt caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcttcgctat
      301 tacgccagct ggcgaaaggg ggatgtgctg caaggcgatt aagttgggta acgccagggt
      361 tttcccagtc acgacgttgt aaaacgacgg ccagtgaatt cgagctcggt acccggggat
      421 cctctagagt cgacctgcag gcatgcaagc ttggcgtaat catggtcata gctgtttcct
      481 gtgtgaaatt gttatccgct cacaattcca cacaacatac gagccggaag cataaagtgt
      541 aaagcctggg gtgcctaatg agtgagctaa ctcacattaa ttgcgttgcg ctcactgccc
      601 gctttccagt cgggaaacct gtcgtgccag ctgcattaat gaatcggcca acgcgcgggg
      661 agaggcggtt tgcgtattgg gcgctcttcc gctggtctcc gacttccatc atagttatgg
      721 ccatgactac tctagctagc agtgttaaat cattcagcta cctgagaggg gcccctataa
      781 ctctctacgg ctaacctgaa tggagccacc tgagaccgct tcctcgctca ctgactcgct
      841 gcgctcggtc gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg taatacggtt
      901 atccacagaa tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc
      961 caggaaccgt aaaaaggccg cgttgctggc gtttttccat aggctccgcc cccctgacga
     1021 gcatcacaaa aatcgacgct caagtcagag gtggcgaaac ccgacaggac tataaagata
     1081 ccaggcgttt ccccctggaa gctccctcgt gcgctctcct gttccgaccc tgccgcttac
     1141 cggatacctg tccgcctttc tcccttcggg aagcgtggcg ctttctcata gctcacgctg
     1201 taggtatctc agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc
     1261 cgttcagccc gaccgctgcg ccttatccgg taactatcgt cttgagtcca acccggtaag
     1321 acacgactta tcgccactgg cagcagccac tggtaacagg attagcagag cgaggtatgt
     1381 aggcggtgct acagagttct tgaagtggtg gcctaactac ggctacacta gaagaacagt
     1441 atttggtatc tgcgctctgc tgaagccagt taccttcgga aaaagagttg gtagctcttg
     1501 atccggcaaa caaaccaccg ctggtagcgg tggttttttt gtttgcaagc agcagattac
     1561 gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt tctacggggt ctgacgctca
     1621 gtggaacgaa aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac
     1681 ctagatcctt ttaaattaaa aatgaagttt taaatcaatc taaagtatat atgagtaaac
     1741 ttggtctgac agttaccaat gcttaatcag tgaggcacct atctcagcga tctgtctatt
     1801 tcgttcatcc atagttgcct gactccccgt cgtgtagata actacgatac gggagggctt
     1861 accatctggc cccagtgctg caatgatacc gcgagaccca cgctcaccgg ctccagattt
     1921 atcagcaata aaccagccag ccggaagggc cgagcgcaga agtggtcctg caactttatc
     1981 cgcctccatc cagtctatta attgttgccg ggaagctaga gtaagtagtt cgccagttaa
     2041 tagtttgcgc aacgttgttg ccattgctac aggcatcgtg gtgtcacgct cgtcgtttgg
     2101 tatggcttca ttcagctccg gttcccaacg atcaaggcga gttacatgat cccccatgtt
     2161 gtgcaaaaaa gcggttagct ccttcggtcc tccgatcgtt gtcagaagta agttggccgc
     2221 agtgttatca ctcatggtta tggcagcact gcataattct cttactgtca tgccatccgt
     2281 aagatgcttt tctgtgactg gtgagtactc aaccaagtca ttctgagaat agtgtatgcg
     2341 gcgaccgagt tgctcttgcc cggcgtcaat acgggataat accgcgccac atagcagaac
     2401 tttaaaagtg ctcatcattg gaaaacgttc ttcggggcga aaactctcaa ggatcttacc
     2461 gctgttgaga tccagttcga tgtaacccac tcgtgcaccc aactgatctt cagcatcttt
     2521 tactttcacc agcgtttctg ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg
     2581 aataagggcg acacggaaat gttgaatact catactcttc ctttttcaat attattgaag
     2641 catttatcag ggttattgtc tcatgagcgg atacatattt gaatgtattt agaaaaataa
     2701 acaaataggg gttccgcgca catttccccg aaaagtgcca cctgacgtct aagaaaccat
     2761 tattatcatg acattaacct ataaaaatag gcgtatcacg aggccctttc gtc
//
