LOCUS       pTW083_Lvl0_-98/        2822 bp    DNA     circular     28-JAN-2014
DEFINITION  Cloning vector pUC19, complete sequence.
ACCESSION   urn.local...hu-9e4hqcb
KEYWORDS    .
SOURCE      Cloning vector pUC19
  ORGANISM  Cloning vector pUC19
            other sequences; artificial sequences; vectors.
REFERENCE   1  (bases 1 to 2686)
  AUTHORS   New, England and Biolabs.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-OCT-2007) Research Department, New England Biolabs,
            240 County Road, Ipswich, MA 01938, USA COMMENT See also GenBank
            accession L09137.
FEATURES             Location/Qualifiers
     source          1..2822
                     /organism="Cloning vector pUC19"
                     /mol_type="other DNA"
     gene            55..655
                     /standard_name="lacZ PCR Product from pUC19"
     primer_bind     55..75
                     /standard_name="TK_DI_lacZ_Fwd_1"
     CDS             complement(146..469)
                     /gene="lacZalpha"
                     /codon_start=1
                     /product="beta-galactosidase alpha fragment"
                     /translation="MTMITPSLHACRSTLEDPRVPSSNSLAVVLQRRDWENPGVTQLN
                     RLAAHPPFASWRNSEEARTDRPSQQLRSLNGEWRLMRYFLLTHLCGISHRIWCTLSTI
                     CSDAA"
     gene            complement(146..469)
                     /gene="lacZalpha"
     primer_bind     364..386
                     /standard_name="M13/pUC Forward (Quintara)"
     primer_bind     378..395
                     /standard_name="M13 (-21) Forward (Quintara)"
     misc_feature    396..452
                     /gene="lacZalpha"
                     /note="multiple cloning site (EcoRI-HindIII)"
                     /note="Geneious type: polylinker"
     primer_bind     complement(465..481)
                     /standard_name="M13 Reverse (Quintara)"
     -10_signal      complement(514..519)
                     /note="Plac promoter (drives lacZalpha;
                     counter-clockwise); TATGTT"
     -35_signal      complement(538..543)
                     /note="Plac promoter (drives lacZalpha;
                     counter-clockwise); TTTACA"
     protein_bind    complement(563..575)
                     /note="CAP protein binding site"
                     /bound_moiety="catabolite activator protein (CAP)"
     primer_bind     578..598
                     /standard_name="TW_lvl0_seq_Fwd1"
     misc_feature    679..693
                     /standard_name="15 bp SapI Overhang"
     misc_feature    694..699
                     /standard_name="BsaI"
     primer_bind     694..826
                     /standard_name="TW_lvl0_98-14_NA_Fwd"
     primer_bind     complement(694..826)
                     /standard_name="TW_lvl0_98-14_NA_Rev"
     misc_feature    701..704
                     /standard_name="BsaI Cut Site"
     misc_feature    702..804
                     /standard_name="98/5 SGP"
     primer_bind     719..740
                     /standard_name="TK-VEE-nsP4-seq-F"
     primer_bind     755..769
                     /standard_name="ApaI 15 bp Overhang"
     misc_feature    800..>813
                     /standard_name="Subgenomic RNA 5'UTR"
     misc_feature    813^814
                     /note="Geneious type: Editing History Deletion"
                     /standard_name="AGTCTAGTCCGCCAAG"
     misc_feature    complement(816..819)
                     /standard_name="BsaI Cut Site"
     misc_feature    complement(821..826)
                     /standard_name="BsaI"
     primer_bind     complement(912..932)
                     /standard_name="TW_lvl0_seq_Rev1"
     rep_origin      complement(944..1617)
     misc_RNA        complement(1003..1555)
                     /note="RNAII transcript (complementary strand)"
     rep_origin      complement(1003..1591)
     rep_origin      complement(1003..1536)
     primer_bind     complement(1042..1062)
                     /standard_name="TW_HDV-seq-R"
     primer_bind     1043..1064
                     /standard_name="pHAGE-A7550-7050"
     primer_bind     complement(1307..1331)
                     /standard_name="pHAGE-S7501-8000"
     primer_bind     complement(1351..1370)
                     /standard_name="RepO-R"
     -35_signal      1409..1414
                     /note="RNAI promoter (clockwise); TTGAAG"
     -10_signal      1431..1436
                     /note="RNAI promoter (clockwise); GCTACA"
     misc_RNA        1445..1552
                     /note="RNAI transcript"
     primer_bind     1554..1575
                     /standard_name="pHAGE-A7050-6550"
     -10_signal      complement(1565..1570)
                     /note="RNAII promoter (counter-clockwise); CGTAAT"
     -35_signal      complement(1586..1591)
                     /note="RNAII promoter (counter-clockwise); TTGAGA"
     CDS             complement(1762..2622)
                     /gene="bla"
                     /note="ampR (confers resistance to ampicillin)"
                     /codon_start=1
                     /product="beta-lactamase"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE
                     YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
                     DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
                     LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
                     EIGASLIKHW"
     gene            complement(1762..2622)
                     /gene="bla"
     primer_bind     complement(1811..1835)
                     /standard_name="pHAGE-S7001-7500"
     misc_feature    complement(1902..1907)
                     /standard_name="BsaI site"
     primer_bind     2063..2086
                     /standard_name="pHAGE-A6550-6050"
     primer_bind     complement(2331..2355)
                     /standard_name="pHAGE-S6501-7000"
     primer_bind     2385..2404
                     /standard_name="Amp-R; Quintara AmpR"
     primer_bind     2532..2555
                     /standard_name="pHAGE-A6050-5550"
     sig_peptide     complement(2554..2622)
                     /gene="bla"
                     /note="required for secretion to the periplasm; cleaved
                     off to form the mature beta-lactamase protein."
     promoter        complement(2623..2657)
                     /note="Beta Lactamase Promoter"
                     /standard_name="P-Bla"
     -10_signal      complement(2666..2671)
                     /note="bla promoter (counter-clockwise); GAGACA"
     -35_signal      complement(2687..2692)
                     /note="bla promoter (counter-clockwise); TTCAAA"
ORIGIN      
        1 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca
       61 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg
      121 ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc
      181 accatatgcg gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc atcaggcgcc
      241 attcgccatt caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcttcgctat
      301 tacgccagct ggcgaaaggg ggatgtgctg caaggcgatt aagttgggta acgccagggt
      361 tttcccagtc acgacgttgt aaaacgacgg ccagtgaatt cgagctcggt acccggggat
      421 cctctagagt cgacctgcag gcatgcaagc ttggcgtaat catggtcata gctgtttcct
      481 gtgtgaaatt gttatccgct cacaattcca cacaacatac gagccggaag cataaagtgt
      541 aaagcctggg gtgcctaatg agtgagctaa ctcacattaa ttgcgttgcg ctcactgccc
      601 gctttccagt cgggaaacct gtcgtgccag ctgcattaat gaatcggcca acgcgcgggg
      661 agaggcggtt tgcgtattgg gcgctcttcc gctggtctcc gacttccatc atagttatgg
      721 ccatgactac tctagctagc agtgttaaat cattcagcta cctgagaggg gcccctataa
      781 ctctctacgg ctaacctgaa tggactacga catgccacct gagaccgctt cctcgctcac
      841 tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta tcagctcact caaaggcggt
      901 aatacggtta tccacagaat caggggataa cgcaggaaag aacatgtgag caaaaggcca
      961 gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg tttttccata ggctccgccc
     1021 ccctgacgag catcacaaaa atcgacgctc aagtcagagg tggcgaaacc cgacaggact
     1081 ataaagatac caggcgtttc cccctggaag ctccctcgtg cgctctcctg ttccgaccct
     1141 gccgcttacc ggatacctgt ccgcctttct cccttcggga agcgtggcgc tttctcatag
     1201 ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca
     1261 cgaacccccc gttcagcccg accgctgcgc cttatccggt aactatcgtc ttgagtccaa
     1321 cccggtaaga cacgacttat cgccactggc agcagccact ggtaacagga ttagcagagc
     1381 gaggtatgta ggcggtgcta cagagttctt gaagtggtgg cctaactacg gctacactag
     1441 aagaacagta tttggtatct gcgctctgct gaagccagtt accttcggaa aaagagttgg
     1501 tagctcttga tccggcaaac aaaccaccgc tggtagcggt ggtttttttg tttgcaagca
     1561 gcagattacg cgcagaaaaa aaggatctca agaagatcct ttgatctttt ctacggggtc
     1621 tgacgctcag tggaacgaaa actcacgtta agggattttg gtcatgagat tatcaaaaag
     1681 gatcttcacc tagatccttt taaattaaaa atgaagtttt aaatcaatct aaagtatata
     1741 tgagtaaact tggtctgaca gttaccaatg cttaatcagt gaggcaccta tctcagcgat
     1801 ctgtctattt cgttcatcca tagttgcctg actccccgtc gtgtagataa ctacgatacg
     1861 ggagggctta ccatctggcc ccagtgctgc aatgataccg cgagacccac gctcaccggc
     1921 tccagattta tcagcaataa accagccagc cggaagggcc gagcgcagaa gtggtcctgc
     1981 aactttatcc gcctccatcc agtctattaa ttgttgccgg gaagctagag taagtagttc
     2041 gccagttaat agtttgcgca acgttgttgc cattgctaca ggcatcgtgg tgtcacgctc
     2101 gtcgtttggt atggcttcat tcagctccgg ttcccaacga tcaaggcgag ttacatgatc
     2161 ccccatgttg tgcaaaaaag cggttagctc cttcggtcct ccgatcgttg tcagaagtaa
     2221 gttggccgca gtgttatcac tcatggttat ggcagcactg cataattctc ttactgtcat
     2281 gccatccgta agatgctttt ctgtgactgg tgagtactca accaagtcat tctgagaata
     2341 gtgtatgcgg cgaccgagtt gctcttgccc ggcgtcaata cgggataata ccgcgccaca
     2401 tagcagaact ttaaaagtgc tcatcattgg aaaacgttct tcggggcgaa aactctcaag
     2461 gatcttaccg ctgttgagat ccagttcgat gtaacccact cgtgcaccca actgatcttc
     2521 agcatctttt actttcacca gcgtttctgg gtgagcaaaa acaggaaggc aaaatgccgc
     2581 aaaaaaggga ataagggcga cacggaaatg ttgaatactc atactcttcc tttttcaata
     2641 ttattgaagc atttatcagg gttattgtct catgagcgga tacatatttg aatgtattta
     2701 gaaaaataaa caaatagggg ttccgcgcac atttccccga aaagtgccac ctgacgtcta
     2761 agaaaccatt attatcatga cattaaccta taaaaatagg cgtatcacga ggccctttcg
     2821 tc
//
