LOCUS       pTW084_Lvl0_-98/        2823 bp    DNA     circular     28-JAN-2014
DEFINITION  Cloning vector pUC19, complete sequence.
ACCESSION   urn.local...hv-9e4hqcb
KEYWORDS    .
SOURCE      Cloning vector pUC19
  ORGANISM  Cloning vector pUC19
            other sequences; artificial sequences; vectors.
REFERENCE   1  (bases 1 to 2686)
  AUTHORS   New, England and Biolabs.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-OCT-2007) Research Department, New England Biolabs,
            240 County Road, Ipswich, MA 01938, USA COMMENT See also GenBank
            accession L09137.
FEATURES             Location/Qualifiers
     source          1..2823
                     /organism="Cloning vector pUC19"
                     /mol_type="other DNA"
     gene            55..655
                     /standard_name="lacZ PCR Product from pUC19"
     primer_bind     55..75
                     /standard_name="TK_DI_lacZ_Fwd_1"
     CDS             complement(146..469)
                     /gene="lacZalpha"
                     /codon_start=1
                     /product="beta-galactosidase alpha fragment"
                     /translation="MTMITPSLHACRSTLEDPRVPSSNSLAVVLQRRDWENPGVTQLN
                     RLAAHPPFASWRNSEEARTDRPSQQLRSLNGEWRLMRYFLLTHLCGISHRIWCTLSTI
                     CSDAA"
     gene            complement(146..469)
                     /gene="lacZalpha"
     primer_bind     364..386
                     /standard_name="M13/pUC Forward (Quintara)"
     primer_bind     378..395
                     /standard_name="M13 (-21) Forward (Quintara)"
     misc_feature    396..452
                     /gene="lacZalpha"
                     /note="multiple cloning site (EcoRI-HindIII)"
                     /note="Geneious type: polylinker"
     primer_bind     complement(465..481)
                     /standard_name="M13 Reverse (Quintara)"
     -10_signal      complement(514..519)
                     /note="Plac promoter (drives lacZalpha;
                     counter-clockwise); TATGTT"
     -35_signal      complement(538..543)
                     /note="Plac promoter (drives lacZalpha;
                     counter-clockwise); TTTACA"
     protein_bind    complement(563..575)
                     /note="CAP protein binding site"
                     /bound_moiety="catabolite activator protein (CAP)"
     primer_bind     578..598
                     /standard_name="TW_lvl0_seq_Fwd1"
     misc_feature    679..693
                     /standard_name="15 bp SapI Overhang"
     misc_feature    694..699
                     /standard_name="BsaI"
     primer_bind     694..827
                     /standard_name="TW_lvl0_98-15_NA_Fwd"
     primer_bind     complement(694..827)
                     /standard_name="TW_lvl0_98-15_NA_Rev"
     misc_feature    701..704
                     /standard_name="BsaI Cut Site"
     misc_feature    702..804
                     /standard_name="98/5 SGP"
     primer_bind     719..740
                     /standard_name="TK-VEE-nsP4-seq-F"
     primer_bind     755..769
                     /standard_name="ApaI 15 bp Overhang"
     misc_feature    800..>814
                     /standard_name="Subgenomic RNA 5'UTR"
     misc_feature    814^815
                     /note="Geneious type: Editing History Deletion"
                     /standard_name="GTCTAGTCCGCCAAG"
     misc_feature    complement(817..820)
                     /standard_name="BsaI Cut Site"
     misc_feature    complement(822..827)
                     /standard_name="BsaI"
     primer_bind     complement(913..933)
                     /standard_name="TW_lvl0_seq_Rev1"
     rep_origin      complement(945..1618)
     misc_RNA        complement(1004..1556)
                     /note="RNAII transcript (complementary strand)"
     rep_origin      complement(1004..1592)
     rep_origin      complement(1004..1537)
     primer_bind     complement(1043..1063)
                     /standard_name="TW_HDV-seq-R"
     primer_bind     1044..1065
                     /standard_name="pHAGE-A7550-7050"
     primer_bind     complement(1308..1332)
                     /standard_name="pHAGE-S7501-8000"
     primer_bind     complement(1352..1371)
                     /standard_name="RepO-R"
     -35_signal      1410..1415
                     /note="RNAI promoter (clockwise); TTGAAG"
     -10_signal      1432..1437
                     /note="RNAI promoter (clockwise); GCTACA"
     misc_RNA        1446..1553
                     /note="RNAI transcript"
     primer_bind     1555..1576
                     /standard_name="pHAGE-A7050-6550"
     -10_signal      complement(1566..1571)
                     /note="RNAII promoter (counter-clockwise); CGTAAT"
     -35_signal      complement(1587..1592)
                     /note="RNAII promoter (counter-clockwise); TTGAGA"
     CDS             complement(1763..2623)
                     /gene="bla"
                     /note="ampR (confers resistance to ampicillin)"
                     /codon_start=1
                     /product="beta-lactamase"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE
                     YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
                     DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
                     LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
                     EIGASLIKHW"
     gene            complement(1763..2623)
                     /gene="bla"
     primer_bind     complement(1812..1836)
                     /standard_name="pHAGE-S7001-7500"
     misc_feature    complement(1903..1908)
                     /standard_name="BsaI site"
     primer_bind     2064..2087
                     /standard_name="pHAGE-A6550-6050"
     primer_bind     complement(2332..2356)
                     /standard_name="pHAGE-S6501-7000"
     primer_bind     2386..2405
                     /standard_name="Amp-R; Quintara AmpR"
     primer_bind     2533..2556
                     /standard_name="pHAGE-A6050-5550"
     sig_peptide     complement(2555..2623)
                     /gene="bla"
                     /note="required for secretion to the periplasm; cleaved
                     off to form the mature beta-lactamase protein."
     promoter        complement(2624..2658)
                     /note="Beta Lactamase Promoter"
                     /standard_name="P-Bla"
     -10_signal      complement(2667..2672)
                     /note="bla promoter (counter-clockwise); GAGACA"
     -35_signal      complement(2688..2693)
                     /note="bla promoter (counter-clockwise); TTCAAA"
ORIGIN      
        1 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca
       61 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg
      121 ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc
      181 accatatgcg gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc atcaggcgcc
      241 attcgccatt caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcttcgctat
      301 tacgccagct ggcgaaaggg ggatgtgctg caaggcgatt aagttgggta acgccagggt
      361 tttcccagtc acgacgttgt aaaacgacgg ccagtgaatt cgagctcggt acccggggat
      421 cctctagagt cgacctgcag gcatgcaagc ttggcgtaat catggtcata gctgtttcct
      481 gtgtgaaatt gttatccgct cacaattcca cacaacatac gagccggaag cataaagtgt
      541 aaagcctggg gtgcctaatg agtgagctaa ctcacattaa ttgcgttgcg ctcactgccc
      601 gctttccagt cgggaaacct gtcgtgccag ctgcattaat gaatcggcca acgcgcgggg
      661 agaggcggtt tgcgtattgg gcgctcttcc gctggtctcc gacttccatc atagttatgg
      721 ccatgactac tctagctagc agtgttaaat cattcagcta cctgagaggg gcccctataa
      781 ctctctacgg ctaacctgaa tggactacga catagccacc tgagaccgct tcctcgctca
      841 ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg
      901 taatacggtt atccacagaa tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc
      961 agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc gtttttccat aggctccgcc
     1021 cccctgacga gcatcacaaa aatcgacgct caagtcagag gtggcgaaac ccgacaggac
     1081 tataaagata ccaggcgttt ccccctggaa gctccctcgt gcgctctcct gttccgaccc
     1141 tgccgcttac cggatacctg tccgcctttc tcccttcggg aagcgtggcg ctttctcata
     1201 gctcacgctg taggtatctc agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc
     1261 acgaaccccc cgttcagccc gaccgctgcg ccttatccgg taactatcgt cttgagtcca
     1321 acccggtaag acacgactta tcgccactgg cagcagccac tggtaacagg attagcagag
     1381 cgaggtatgt aggcggtgct acagagttct tgaagtggtg gcctaactac ggctacacta
     1441 gaagaacagt atttggtatc tgcgctctgc tgaagccagt taccttcgga aaaagagttg
     1501 gtagctcttg atccggcaaa caaaccaccg ctggtagcgg tggttttttt gtttgcaagc
     1561 agcagattac gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt tctacggggt
     1621 ctgacgctca gtggaacgaa aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa
     1681 ggatcttcac ctagatcctt ttaaattaaa aatgaagttt taaatcaatc taaagtatat
     1741 atgagtaaac ttggtctgac agttaccaat gcttaatcag tgaggcacct atctcagcga
     1801 tctgtctatt tcgttcatcc atagttgcct gactccccgt cgtgtagata actacgatac
     1861 gggagggctt accatctggc cccagtgctg caatgatacc gcgagaccca cgctcaccgg
     1921 ctccagattt atcagcaata aaccagccag ccggaagggc cgagcgcaga agtggtcctg
     1981 caactttatc cgcctccatc cagtctatta attgttgccg ggaagctaga gtaagtagtt
     2041 cgccagttaa tagtttgcgc aacgttgttg ccattgctac aggcatcgtg gtgtcacgct
     2101 cgtcgtttgg tatggcttca ttcagctccg gttcccaacg atcaaggcga gttacatgat
     2161 cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc tccgatcgtt gtcagaagta
     2221 agttggccgc agtgttatca ctcatggtta tggcagcact gcataattct cttactgtca
     2281 tgccatccgt aagatgcttt tctgtgactg gtgagtactc aaccaagtca ttctgagaat
     2341 agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaat acgggataat accgcgccac
     2401 atagcagaac tttaaaagtg ctcatcattg gaaaacgttc ttcggggcga aaactctcaa
     2461 ggatcttacc gctgttgaga tccagttcga tgtaacccac tcgtgcaccc aactgatctt
     2521 cagcatcttt tactttcacc agcgtttctg ggtgagcaaa aacaggaagg caaaatgccg
     2581 caaaaaaggg aataagggcg acacggaaat gttgaatact catactcttc ctttttcaat
     2641 attattgaag catttatcag ggttattgtc tcatgagcgg atacatattt gaatgtattt
     2701 agaaaaataa acaaataggg gttccgcgca catttccccg aaaagtgcca cctgacgtct
     2761 aagaaaccat tattatcatg acattaacct ataaaaatag gcgtatcacg aggccctttc
     2821 gtc
//
