LOCUS       pTW927                  4237 bp    DNA     circular     17-JAN-2020
DEFINITION  Cloning vector pUC19, complete sequence.
ACCESSION   urn.local...q-a4y51ct
KEYWORDS    .
SOURCE      Cloning vector pUC19
  ORGANISM  Cloning vector pUC19
            other sequences; artificial sequences; vectors.
REFERENCE   1  (bases 1 to 2686)
  AUTHORS   New, England and Biolabs.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-OCT-2007) Research Department, New England Biolabs,
            240 County Road, Ipswich, MA 01938, USA COMMENT See also GenBank
            accession L09137.
FEATURES             Location/Qualifiers
     gene            complement(146..469)
                     /gene="lacZalpha"
     -10_signal      complement(514..519)
                     /note="Plac promoter (drives lacZalpha;
                     counter-clockwise); TATGTT"
     -35_signal      complement(538..543)
                     /note="Plac promoter (drives lacZalpha;
                     counter-clockwise); TTTACA"
     misc_feature    694..699
                     /created_by="User"
                     /label="BsaI"
     misc_feature    701..704
                     /created_by="User"
                     /label="BsaI Cut Site"
     misc_feature    705..1324
                     /Original_Bases="GTCGACCGACAGCCTTCCAAATGTTCTTCGGGTGATGCTGC
                     CAACTTAGTCGACCGACAGCCTTCCAAATGTTCTTCTCAAACGGAATCGTCGTATCCA
                     GCCTACTCGCTATTGTCCTCAATGCCGTATTAAATCATAAAAAGAAATAAGAAAAAGA
                     GGTGCGAGCCTCTTTTTTGTGTGACAAAATAAAAACATCTACCTATTCATATACGCTA
                     GTGTCATAGTCCTGAAAATCATCTGCATCAAGAACAATTTCACAACTCTTATACTTTT
                     CTCTTACAAGTCGTTCGGCTTCATCTGGATTTTCAGCCTCTATACTTACTAAACGTGA
                     TAAAGTTTCTGTAATTTCTACTGTATCGACCTGCAGACTGGCTGTGTATAAGGGAGCC
                     TGACATTTATATTCCCCAGAACATCAGGTTAATGGCGTTTTTGATGTCATTTTCGCGG
                     TGGCTGAGATCAGCCACTTCTTCCCCGATAACGGAGACCGGCACACTGGCCATATCGG
                     TGGTCATCATGCGCCAGCTTTCATCCCCGATATGCACCACCGGGTAAAGTTCACGGGA
                     GACTTTATCTGACAGCAGACGTGCACTGGCCAGGGGGATCACCATCCGTCGCCCGGGC
                     GTGTCAATAATATCACTCTGTACATCCACAAACAGACGATAACGGCTCTCTCTTTTAT
                     AGGTGTAAACCTTAAACTGCATTTCACCAGCCCCTGTTCTCGTCAGCAAAAGAGCCGT
                     TCATTTCAATAAACCGGGCGACCTCAGCCATCCCTTCCTGATTTTCCGCTTTCCAGCG
                     TTCGGCACGCAGACGACGGGCTTCATTCTGCATGGTTGTGCTTACCAGACCGGAGATA
                     TTGACATCATATATGCCTTGAGCAACTGATAGCTGTCGCTGTCAACTGTCACTGTAAT
                     ACGCTGCTTCATAGCATACCTCTTTTTGACATACTTCGGGTATACATATCAGTATATA
                     TTCTTATACCGCAAAAATCAGCGCGCAAATACGCATACTGTTATCTGGCTTTTAGTAA
                     GCCGGATCCACGCGGCGTTTACGCCCCCCCTGCCACTCATCGCAGTACTGTTGTAATT
                     CATTAAGCATTCTGCCGACATGGAAGCCATCACAAACGGCATGATGAACCTGAATCGC
                     CAGCGGCATCAGCACCTTGTCGCCTTGCGTATAATATTTGCCCATGGTGAAAACGGGG
                     GCGAAGAAGTTGTCCATATTGGCCACGTTTAAATCAAAACTGGTGAAACTCACCCAGG
                     GATTGGCTGAGACGAAAAACATATTCTCAATAAACCCTTTAGGGAAATAGGCCAGGTT
                     TTCACCGTAACACGCCACATCTTGCGAATATATGTGTAGAAACTGCCGGAAATCGTCG
                     TGGTATTCACTCCAGAGCGATGAAAACGTTTCAGTTTGCTCATGGAAAACGGTGTAAC
                     AAGGGTGAACACTATCCCATATCACCAGCTCACCGTCTTTCATTGCCATACGGAATTC
                     CGGATGAGCATTCATCAGGCGGGCAAGAATGTGAATAAAGGCCGGATAAAACTTGTGC
                     TTATTTTTCTTTACGGTCTTTAAAAAGGCCGTAATATCCAGCTGAACGGTCTGGTTAT
                     AGGTACATTGAGCAACTGACTGAAATGCCTCAAAATGTTCTTTACGATGCCATTGGGA
                     TATATCAACGGTGGTATATCCAGTGATTTTTTTCTCCATTTTAGCTTCCTTAGCTCCT
                     GAAAATCTCGATAACTCAAAAAATACGCCCGGTAGTGATCTTATTTCATTATGGTGAA
                     AGTTGGAACCTCTTACGTGCCGATCAACGTCTCATTTTCGCCAAAAGTTGGCCCAGGG
                     CTTCCCGGTATCAACAGGGACACCAGGATTTATTTATTCTGCGAAGTGATCTTCCGTC
                     ACAGGTATTTATTCGGCGCAAAGTGCGTCGGGTGATGCTGCCAACTTAGTCGA"
                     /label="GTCGACCGACAGCCTTCCAAATGTTCTTCGGGTGATGCTGCCAACTTAGT
                     CGACCGACAGCCTTCCAAATGTTCTTCTCAAACGGAATCGTCGTATCCAGCCTACTCG
                     CTATTGTCCTCAATGCCGTATTAAATCATAAAAAGAAATAAGAAAAAGAGGTGCGAGC
                     CTCTTTTTTGTGTGACAAAATAAAAACATCTACCTATTCATATACGCTAGTGTCATAG
                     TCCTGAAAATCATCTGCATCAAGAACAATTTCACAACTCTTATACTTTTCTCTTACAA
                     GTCGTTCGGCTTCATCTGGATTTTCAGCCTCTATACTTACTAAACGTGATAAAGTTTC
                     TGTAATTTCTACTGTATCGACCTGCAGACTGGCTGTGTATAAGGGAGCCTGACATTTA
                     TATTCCCCAGAACATCAGGTTAATGGCGTTTTTGATGTCATTTTCGCGGTGGCTGAGA
                     TCAGCCACTTCTTCCCCGATAACGGAGACCGGCACACTGGCCATATCGGTGGTCATCA
                     TGCGCCAGCTTTCATCCCCGATATGCACCACCGGGTAAAGTTCACGGGAGACTTTATC
                     TGACAGCAGACGTGCACTGGCCAGGGGGATCACCATCCGTCGCCCGGGCGTGTCAATA
                     ATATCACTCTGTACATCCACAAACAGACGATAACGGCTCTCTCTTTTATAGGTGTAAA
                     CCTTAAACTGCATTTCACCAGCCCCTGTTCTCGTCAGCAAAAGAGCCGTTCATTTCAA
                     TAAACCGGGCGACCTCAGCCATCCCTTCCTGATTTTCCGCTTTCCAGCGTTCGGCACG
                     CAGACGACGGGCTTCATTCTGCATGGTTGTGCTTACCAGACCGGAGATATTGACATCA
                     TATATGCCTTGAGCAACTGATAGCTGTCGCTGTCAACTGTCACTGTAATACGCTGCTT
                     CATAGCATACCTCTTTTTGACATACTTCGGGTATACATATCAGTATATATTCTTATAC
                     CGCAAAAATCAGCGCGCAAATACGCATACTGTTATCTGGCTTTTAGTAAGCCGGATCC
                     ACGCGGCGTTTACGCCCCCCCTGCCACTCATCGCAGTACTGTTGTAATTCATTAAGCA
                     TTCTGCCGACATGGAAGCCATCACAAACGGCATGATGAACCTGAATCGCCAGCGGCAT
                     CAGCACCTTGTCGCCTTGCGTATAATATTTGCCCATGGTGAAAACGGGGGCGAAGAAG
                     TTGTCCATATTGGCCACGTTTAAATCAAAACTGGTGAAACTCACCCAGGGATTGGCTG
                     AGACGAAAAACATATTCTCAATAAACCCTTTAGGGAAATAGGCCAGGTTTTCACCGTA
                     ACACGCCACATCTTGCGAATATATGTGTAGAAACTGCCGGAAATCGTCGTGGTATTCA
                     CTCCAGAGCGATGAAAACGTTTCAGTTTGCTCATGGAAAACGGTGTAACAAGGGTGAA
                     CACTATCCCATATCACCAGCTCACCGTCTTTCATTGCCATACGGAATTCCGGATGAGC
                     ATTCATCAGGCGGGCAAGAATGTGAATAAAGGCCGGATAAAACTTGTGCTTATTTTTC
                     TTTACGGTCTTTAAAAAGGCCGTAATATCCAGCTGAACGGTCTGGTTATAGGTACATT
                     GAGCAACTGACTGAAATGCCTCAAAATGTTCTTTACGATGCCATTGGGATATATCAAC
                     GGTGGTATATCCAGTGATTTTTTTCTCCATTTTAGCTTCCTTAGCTCCTGAAAATCTC
                     GATAACTCAAAAAATACGCCCGGTAGTGATCTTATTTCATTATGGTGAAAGTTGGAAC
                     CTCTTACGTGCCGATCAACGTCTCATTTTCGCCAAAAGTTGGCCCAGGGCTTCCCGGT
                     ATCAACAGGGACACCAGGATTTATTTATTCTGCGAAGTGATCTTCCGTCACAGGTATT
                     TATTCGGCGCAAAGTGCGTCGGGTGATGCTGCCAACTTAGTCGA"
                     /note="Geneious type: Editing History Replacement"
     gene            705..>1325
                     /created_by="User"
                     /label="TetR"
     misc_feature    1326..1334
                     /created_by="User"
                     /label="GSG linker"
     CDS             <1335..1391
                     /created_by="User"
                     /label="P2A"
     source          <1392..>2102
                     /organism="Cloning vector pUC19"
                     /mol_type="other DNA"
     CDS             1392..>2102
                     /created_by="User"
                     /X-Transferred_From="pTK035; pTK117"
                     /X-Transferred_Similarity="100.00%"
                     /Transferred_From="pTK035, pTK117, pTW025 Triple -241/+30
                     EBFP No SGP Yet, pTW031 short HDV Active (-), pTW032 short
                     HDV Inactive (-), pTW033 Long HDV Active (-), pTW034 Long
                     HDV Inactive (-), pTW035 Long HDV Active Minus C (-),
                     pTW036 Long HDV Inactive Minus C (-), pTW039 Genomic HDV
                     Active (-), pTW040 Genomic HDV Inactive (-)"
                     /Transferred_Similarity="100.00%"
                     /label="mKate"
     misc_feature    1644..1654
                     /Recognition_pattern="(8/13)AAGNNNNNCTT(13/8)"
                     /annotation_group="AAGNNNNNCTT"
                     /Transferred_From="pTW031 short HDV Active (-), pTW039
                     Genomic HDV Active (-), pTW040 Genomic HDV Inactive (-)"
                     /Transferred_Similarity="100.00%"
                     /label="FalI"
                     /note="Geneious type: restriction site"
     misc_feature    1775..1787
                     /Recognition_pattern="(7/12)GAACNNNNNNTCC(12/7)"
                     /annotation_group="GAACNNNNNNTCC"
                     /Transferred_From="pTW031 short HDV Active (-), pTW039
                     Genomic HDV Active (-), pTW040 Genomic HDV Inactive (-)"
                     /Transferred_Similarity="100.00%"
                     /label="AloI"
                     /note="Geneious type: restriction site"
     misc_feature    1861..1871
                     /Recognition_pattern="GCCNNNN^NGGC"
                     /annotation_group="GCCNNNNNGGC"
                     /Transferred_From="pTW031 short HDV Active (-), pTW039
                     Genomic HDV Active (-), pTW040 Genomic HDV Inactive (-)"
                     /Transferred_Similarity="100.00%"
                     /label="BglI"
                     /note="Geneious type: restriction site"
     source          <2103..>2230
                     /organism="Cloning vector pUC19"
                     /mol_type="other DNA"
     CDS             2103..2228
                     /Original_Bases
                     /modified_by="User"
                     /Transferred_From="pJB052"
                     /Transferred_Similarity="100.00%"
                     /label="PEST"
     gene            2231
                     /created_by="User"
                     /label="mVenus"
     misc_feature    complement(2231..2234)
                     /created_by="User"
                     /label="BsaI Cut Site"
     misc_feature    complement(2236..2241)
                     /created_by="User"
                     /label="BsaI"
     rep_origin      complement(2359..3032)
                     /created_by="User"
                     /modified_by="User"
                     /Transferred_From="pDONR221, pTK247"
                     /Transferred_Similarity="100.00%"
                     /label="pUC origin"
     misc_RNA        complement(2418..2970)
                     /note="RNAII transcript (complementary strand)"
     misc_feature    2439..2450
                     /Recognition_pattern="(10/12)TGANNNNNNTCA(12/10)"
                     /annotation_group="TGANNNNNNTCA"
                     /Transferred_From="pTW031 short HDV Active (-), pTW039
                     Genomic HDV Active (-), pTW040 Genomic HDV Inactive (-)"
                     /Transferred_Similarity="100.00%"
                     /label="BdaI"
                     /note="Geneious type: restriction site"
     misc_feature    2459..2470
                     /Recognition_pattern="GACNNNN^NNGTC"
                     /annotation_group="GACNNNNNNGTC"
                     /Transferred_From="pTW031 short HDV Active (-), pTW039
                     Genomic HDV Active (-), pTW040 Genomic HDV Inactive (-)"
                     /Transferred_Similarity="100.00%"
                     /label="DrdI, AasI, DseDI"
                     /note="Geneious type: restriction site"
     -35_signal      2824..2829
                     /note="RNAI promoter (clockwise); TTGAAG"
     -10_signal      2846..2851
                     /note="RNAI promoter (clockwise); GCTACA"
     misc_RNA        2860..2967
                     /note="RNAI transcript"
     -10_signal      complement(2980..2985)
                     /note="RNAII promoter (counter-clockwise); CGTAAT"
     -35_signal      complement(3001..3006)
                     /note="RNAII promoter (counter-clockwise); TTGAGA"
     gene            complement(3177..4037)
                     /gene="bla"
     misc_feature    3321
                     /Original_Bases="C"
                     /label="C"
                     /note="Geneious type: Editing History Replacement"
     misc_feature    3364..3374
                     /Recognition_pattern="GCCNNNN^NGGC"
                     /annotation_group="GCCNNNNNGGC"
                     /Transferred_From="pTW031 short HDV Active (-), pTW039
                     Genomic HDV Active (-), pTW040 Genomic HDV Inactive (-)"
                     /Transferred_Similarity="100.00%"
                     /label="BglI"
                     /note="Geneious type: restriction site"
     misc_feature    3845..3854
                     /Recognition_pattern="GAANN^NNTTC"
                     /annotation_group="GAANNNNTTC"
                     /Transferred_From="pTW031 short HDV Active (-), pTW039
                     Genomic HDV Active (-), pTW040 Genomic HDV Inactive (-)"
                     /Transferred_Similarity="100.00%"
                     /label="XmnI, Asp700I, MroXI, PdmI"
                     /note="Geneious type: restriction site"
     sig_peptide     complement(3969..4037)
                     /gene="bla"
                     /note="required for secretion to the periplasm; cleaved
                     off to form the mature beta-lactamase protein."
     -10_signal      complement(4081..4086)
                     /note="bla promoter (counter-clockwise); GAGACA"
     -35_signal      complement(4102..4107)
                     /note="bla promoter (counter-clockwise); TTCAAA"
ORIGIN      
        1 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca
       61 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg
      121 ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc
      181 accatatgcg gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc atcaggcgcc
      241 attcgccatt caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcttcgctat
      301 tacgccagct ggcgaaaggg ggatgtgctg caaggcgatt aagttgggta acgccagggt
      361 tttcccagtc acgacgttgt aaaacgacgg ccagtgaatt cgagctcggt acccggggat
      421 cctctagagt cgacctgcag gcatgcaagc ttggcgtaat catggtcata gctgtttcct
      481 gtgtgaaatt gttatccgct cacaattcca cacaacatac gagccggaag cataaagtgt
      541 aaagcctggg gtgcctaatg agtgagctaa ctcacattaa ttgcgttgcg ctcactgccc
      601 gctttccagt cgggaaacct gtcgtgccag ctgcattaat gaatcggcca acgcgcgggg
      661 agaggcggtt tgcgtattgg gcgctcttcc gctggtctca caccatgtca agactcgaca
      721 agagcaaggt gattaacagt gcactggaac ttctcaatga agttgggatc gaggggctga
      781 ctactagaaa actcgcacag aaactggggg ttgagcagcc caccttgtac tggcacgtta
      841 aaaacaaaag ggccctgctg gatgctctgg ccatcgagat gctggatagg catcataccc
      901 acttctgccc tctggaagga gaatcctggc aggatttcct tagaaacaac gccaagtcct
      961 ttcgctgtgc ccttcttagc caccgggatg gtgctaaagt ccatctcggc acacgaccaa
     1021 ctgagaagca gtacgaaact ctcgagaacc agctggcctt tctctgtcaa cagggctttt
     1081 ctcttgaaaa cgccctgtac gcactgagtg cagttgggca ctttacactc ggatgtgttc
     1141 tggaggacca agaacatcag gtggcaaagg aagagaggga gacacctacg actgactcca
     1201 tgccccctct cttgaggcag gcaatagaat tgttcgacca tcagggcgca gaacccgcct
     1261 ttctgtttgg gctggaactg attatctgcg gtcttgagaa acagctgaag tgcgagtccg
     1321 ggagcggaag cggagctact aacttcagcc tgctgaagca ggctggagac gtggaggaga
     1381 accctggacc tatggtgtct aagggcgagg agctgattaa ggagaacatg cacatgaagc
     1441 tgtacatgga gggcaccgtg aacaaccacc acttcaagtg cacatccgag ggcgaaggca
     1501 agccctacga gggcacccag accatgagaa tcaaggtggt cgagggcggc cctctcccct
     1561 tcgccttcga catcctggct accagcttca tgtacggcag caaaaccttc atcaaccaca
     1621 cccagggcat ccccgacttc tttaagcagt ccttccctga gggcttcaca tgggagagag
     1681 tcaccacata cgaagacggg ggcgtgctga ccgctaccca ggacaccagc ctccaggacg
     1741 gctgcctcat ctacaacgtc aagatcagag gggtgaactt cccatccaac ggccctgtga
     1801 tgcagaagaa aacactcggc tgggaggcct ccaccgagat gctgtacccc gctgacggcg
     1861 gcctggaagg cagaagcgac atggccctga agctcgtggg cgggggccac ctgatctgca
     1921 acttgaagac cacatacaga tccaagaaac ccgctaagaa cctcaagatg cccggcgtct
     1981 actatgtgga cagaagactg gaaagaatca aggaggccga caaagagaca tacgtcgagc
     2041 agcacgaggt ggctgtggcc agatactgcg acctccctag caaactgggg cacaaactta
     2101 ataagcttag ccatggcttc ccgccggagg tggaggagca ggatgatggc acgctgccca
     2161 tgtcttgtgc ccaggagagc gggatggacc gtcaccctgc agcctgtgct tctgctagga
     2221 tcaatgtgta ataatgagac cgcttcctcg ctcactgact cgctgcgctc ggtcgttcgg
     2281 ctgcggcgag cggtatcagc tcactcaaag gcggtaatac ggttatccac agaatcaggg
     2341 gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag
     2401 gccgcgttgc tggcgttttt ccataggctc cgcccccctg acgagcatca caaaaatcga
     2461 cgctcaagtc agaggtggcg aaacccgaca ggactataaa gataccaggc gtttccccct
     2521 ggaagctccc tcgtgcgctc tcctgttccg accctgccgc ttaccggata cctgtccgcc
     2581 tttctccctt cgggaagcgt ggcgctttct catagctcac gctgtaggta tctcagttcg
     2641 gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac cccccgttca gcccgaccgc
     2701 tgcgccttat ccggtaacta tcgtcttgag tccaacccgg taagacacga cttatcgcca
     2761 ctggcagcag ccactggtaa caggattagc agagcgaggt atgtaggcgg tgctacagag
     2821 ttcttgaagt ggtggcctaa ctacggctac actagaagaa cagtatttgg tatctgcgct
     2881 ctgctgaagc cagttacctt cggaaaaaga gttggtagct cttgatccgg caaacaaacc
     2941 accgctggta gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga
     3001 tctcaagaag atcctttgat cttttctacg gggtctgacg ctcagtggaa cgaaaactca
     3061 cgttaaggga ttttggtcat gagattatca aaaaggatct tcacctagat ccttttaaat
     3121 taaaaatgaa gttttaaatc aatctaaagt atatatgagt aaacttggtc tgacagttac
     3181 caatgcttaa tcagtgaggc acctatctca gcgatctgtc tatttcgttc atccatagtt
     3241 gcctgactcc ccgtcgtgta gataactacg atacgggagg gcttaccatc tggccccagt
     3301 gctgcaatga taccgcgaga tccacgctca ccggctccag atttatcagc aataaaccag
     3361 ccagccggaa gggccgagcg cagaagtggt cctgcaactt tatccgcctc catccagtct
     3421 attaattgtt gccgggaagc tagagtaagt agttcgccag ttaatagttt gcgcaacgtt
     3481 gttgccattg ctacaggcat cgtggtgtca cgctcgtcgt ttggtatggc ttcattcagc
     3541 tccggttccc aacgatcaag gcgagttaca tgatccccca tgttgtgcaa aaaagcggtt
     3601 agctccttcg gtcctccgat cgttgtcaga agtaagttgg ccgcagtgtt atcactcatg
     3661 gttatggcag cactgcataa ttctcttact gtcatgccat ccgtaagatg cttttctgtg
     3721 actggtgagt actcaaccaa gtcattctga gaatagtgta tgcggcgacc gagttgctct
     3781 tgcccggcgt caatacggga taataccgcg ccacatagca gaactttaaa agtgctcatc
     3841 attggaaaac gttcttcggg gcgaaaactc tcaaggatct taccgctgtt gagatccagt
     3901 tcgatgtaac ccactcgtgc acccaactga tcttcagcat cttttacttt caccagcgtt
     3961 tctgggtgag caaaaacagg aaggcaaaat gccgcaaaaa agggaataag ggcgacacgg
     4021 aaatgttgaa tactcatact cttccttttt caatattatt gaagcattta tcagggttat
     4081 tgtctcatga gcggatacat atttgaatgt atttagaaaa ataaacaaat aggggttccg
     4141 cgcacatttc cccgaaaagt gccacctgac gtctaagaaa ccattattat catgacatta
     4201 acctataaaa ataggcgtat cacgaggccc tttcgtc
//
