LOCUS       Exported               11391 bp ds-DNA     circular SYN 23-AUG-2018
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11391)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Aug 23, 2018 from SnapGene Viewer 4.2.3
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..11391
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     misc_feature    640..642
                     /label=ACCT-adaptor [*]
     misc_feature    644..2937
                     /label=pTMO5
     misc_feature    2942..5169
                     /label=GR-LhG4_BD
     misc_feature    5174..5820
                     /label=tRBSC
     misc_feature    5825..5844
                     /label=F-H adapter
     misc_feature    5849..5868
                     /label=H-A adapter
     misc_feature    5873..6274
                     /label=pOp6
     misc_feature    6279..6347
                     /label=SP(ER)
     misc_feature    6357..7073
                     /label=mTurquoise2
     misc_feature    7078..7094
                     /label=HDEL
     misc_feature    7099..7726
                     /label=tUBQ10
     misc_feature    7731..9361
                     /label=SulfR
ORIGIN
        1 tttttatccc cggaagcctg tggatagagg gtagttatcc acgtgaaacc gctaatgccc
       61 cgcaaagcct tgattcacgg ggctttccgg cccgctccaa aaactatcca cgtgaaatcg
      121 ctaatcaggg tacgtgaaat cgctaatcgg agtacgtgaa atcgctaata aggtcacgtg
      181 aaatcgctaa tcaaaaaggc acgtgagaac gctaatagcc ctttcagatc aacagcttgc
      241 aaacacccct cgctccggca agtagttaca gcaagtagta tgttcaatta gcttttcaat
      301 tatgaatata tatatcaatt attggtcgcc cttggcttgt ggacaatgcg ctacgcgcac
      361 cggctccgcc cgtggacaac cgcaagcggt tgcccaccgt cgagcgcctt tgcccacaac
      421 ccggcggccg gccgcaacag atcgttttat aaattttttt ttttgaaaaa gaaaaagccc
      481 gaaaggcggc aacctctcgg gcttctggat ttccgatccc cggaattaga tcttggcagg
      541 atatattgtg gtgtaacgtt ctcgaactag gctcggacga agtaagcggc cgcaacctta
      601 attaaacacg gcgcgccaac agcgatcgca ccaggtacca ccttgttgaa cgtcgtgtgg
      661 gcttcactct gcaccaccac cccaaaaccc tagttcatac cctatcctat aatttatact
      721 caaaattttc tcatttcttc tattatttat caccattcat atggaagtat atatttaatg
      781 aagaattttc ggatctatag atcattatat gtaaaatcag tttttgataa aacatgtgac
      841 agtttcacaa aaatagttca tctttcacaa aaatagtact cgaatccggt agcattttag
      901 tatcctacta tttagttttt ccactccttt tcattatata tataatgata taagtctttt
      961 tacctctttt tcataagtct ttttaactct ttttttcttt aaaaatgatt atatgcacat
     1021 ctacttgata aactggcgga tggtaaactt gagttaggtt tgagaatttt cactaattat
     1081 tttaggattt gaatcttctt tagtatgaga caattaagct aacacactcg aagatatatt
     1141 cagatcataa tcaaaattta tccgttggat atgtatgaat agattaaata aaagagagaa
     1201 tctcttcggt gcacttgaga caaatctata acatagaaaa tcttgattag attaaaaact
     1261 agattaaaga aggaaagaaa aacaagaaag tgggtttggc tatacgaaac gagtgggatt
     1321 attaatgatt aaaagtaaaa gtctttttgg tatctggtcg gtcgacaatc cgtgtgtcct
     1381 gtggtcagtg tttgtttttt attcttttgg ttaattttcg agtattatta tgctttccac
     1441 tgaaaaaaca aaaacattaa gcagccaaat gaaattcttt taacgcgttg tttcagaacc
     1501 tgatgaaaaa acaataatca tgacctcttt cttcttcccc tttaaatgca tgtcaaattg
     1561 tgtaggagtg tttgattata taaattaaat ctactttagt cttctcttct ttgtagaatt
     1621 gtagagtcgt ggtataaact cgaaaatttc ttattatact tatagaaatt aggcagacga
     1681 aatgctcttg atttgcttac aatcttagat taggtttaac ccaagataaa aaaaaacttt
     1741 gttttgatga agaaaaacac cttagttaca attttggaca tcatctttta ttttcacagt
     1801 tagcatctga gacttttttt tcttttctta aatagagttc ttagacaata tcttttgggt
     1861 ccctgatcat gtcaaatgcc aaaaaaataa ataagtatac atgattgtgt ctatttgacc
     1921 attctaactt ctaaaagttt caaatgaaat aactttgtca actattattt ttgcctatga
     1981 attaggtgct gaccagaatc atcttgctaa aatcatgatt ttctactatt gcatattatt
     2041 cttgatcgca tttcacagat cttaatatat acgaaaataa ctgtatatat tatcaactgt
     2101 tacgattaat atataaccat gcatatcgaa atattttgaa ttagtatttt ttttgctaaa
     2161 taatattttg atatagtggt ggcgtggtat tcctcattta caaatcgcat gaactttatc
     2221 ctaaatacga ttaacttaag tagaaaaggc acaaaaatct tctattcatt aacatgaaat
     2281 caattcttaa gaacataaat aaataataat attcatagga agccaaatcc atcagagaaa
     2341 tccacaagat gatgacagct ccccatttag aagtcgtaaa agaaaagcaa agaagatgtg
     2401 cagcacttgc agtttcattt gttttgttag atataccaga aaaaaaaaaa gagtggtccc
     2461 ataaaaacct gtaatatata acattttaat caaagaaaat gatagatttt tttattaaac
     2521 aggaaaaacc actaactttt aattaaaaaa tttggaatac agcaatacat caaaaatcca
     2581 ttaacggtac attatagttg aaaataaatt caggttacat aatatttttt gcgtaatcac
     2641 tatactattc tgccatttta tatggaacta agttcatacg ttatttctca caattctcct
     2701 tgatgtgatc aaagataatg gcggtctgat tgtataaccc ggtgaaaaag gttttttgga
     2761 aagaattctt taatattaaa atatcaaata aattttgttt aaaaattaag gacacattat
     2821 atagcactat atatacacat gtgtatgcat tatcaaatgg gatgtttgtg accttttgtc
     2881 tcttcttctc taaaattctc tttctcccaa aaactaaaaa accaaaaaaa ccaaaaaaac
     2941 accatggcta gtgaagctcg aaaaacaaag aaaaaaatca aagggattca gcaagccact
     3001 gcaggagtct cacaagacac ttcggaaaat cctaacaaaa caatagttcc tgcagcatta
     3061 ccacagctca cccctacctt ggtgtcactg ctggaggtga ttgaacccga ggtgttgtat
     3121 gcaggatatg atagctctgt tccagattca gcatggagaa ttatgaccac actcaacatg
     3181 ttaggtgggc gtcaagtgat tgcagcagtg aaatgggcaa aggcgatacc aggcttcaga
     3241 aacttacacc tggatgacca aatgaccctg ctacagtact catggatgtt tctcatggca
     3301 tttgccctgg gttggagatc atacagacaa tcaagtggaa acctgctctg ctttgctcct
     3361 gatctgatta ttaatgagca gagaatgtct ctaccctgca tgtatgacca atgtaaacac
     3421 atgctgtttg tctcctctga attacaaaga ttgcaggtat cctatgaaga gtatctctgt
     3481 atgaaaacct tactgcttct ctcctcagtt cctaaggaag gtctgaagag ccaagagtta
     3541 tttgatgaga ttcgaatgac ttatatcaaa gagctaggaa aagccatcgt caaaagggaa
     3601 gggaactcca gtcagaactg gcaacggttt taccaactga caaagcttct ggactccatg
     3661 catgaggtgg ttgagaatct ccttacctac tgcttccaga catttttgga taagaccatg
     3721 agtattgaat tcccagagat gttagctgaa atcatcacta atcagatacc aaaatattca
     3781 aatggaaata tcaaaaagct tctgtttcat caaaaatcta ctagcaaacc ggtaacgtta
     3841 tacgacgtcg ctgaatacgc cggcgtttct catcaaaccg tttctagagt ggttaaccag
     3901 gcttcacatg ttagcgctaa aacccgggaa aaagttgaag ctgccatggc tgagctcaac
     3961 tacatcccga accgtgttgc gcagcagctg gctggtaaac aaagcttgct gatcggtgtc
     4021 gcgacctcga gcttggccct gcacgcgccg tcgcaaattg tcgcggcgat taaatctcgc
     4081 gccgatcaac tgggtgccag cgtggtggtg tcgatggtag aacgaagcgg cgtcgaagcc
     4141 tgtaaagcgg cggtgcacaa tcttctcgcg caacgcgtca gtgggctgat cattaactat
     4201 ccgctggatg accaggatgc cattgctgtg gaagctgcct gcactaatgt tccggcgtta
     4261 tttcttgatg tctctgacca gacacccatc aacagtatta ttttctccca tgaagacggt
     4321 acgcgactgg gcgtggagca tctggtcgca ttgggtcacc agcaaatcgc gctgttagcg
     4381 ggcccattaa gttctgtctc ggcgcgtctg cgtctggctg gctggcataa atatctcact
     4441 cgcaatcaaa ttcagccgat agcggaacgg gaaggcgact ggagtgccat gtccggtttt
     4501 caacaaacca tgcaaatgct gaatgagggc atcgttccca ctgcgatgct ggttgccaac
     4561 gatcagatgg cgctgggcgc aatgcgcgcc attaccgagt ccgggctgcg cgttggtgcg
     4621 gatatctcgg tagtgggata cgacgatacc gaagacagct catgttatat cccgccgtta
     4681 accaccatca aacaggattt tcgcctgctg gggcaaacca gcgtggaccg cttgctgcaa
     4741 ctctctcagg gccaggcggt gaagggcaat cagctgttgc ccgtctcact ggtgaaaaga
     4801 aaaaccacta gtggatcgga attcgccaat tttaatcaaa gtgggaatat tgctgatagc
     4861 tcattgtcct tcactttcac taacagtagc aacggtccga acctcataac aactcaaaca
     4921 aattctcaag cgctttcaca accaattgcc tcctctaacg ttcatgataa cttcatgaat
     4981 aatgaaatca cggctagtaa aattgatgat ggtaataatt caaaaccact gtcacctggt
     5041 tggacggacc aaactgcgta taacgcgttt ggaatcacta cagggatgtt taataccact
     5101 acaatggatg atgtatataa ctatctattc gatgatgaag ataccccacc aaacccaaaa
     5161 aaagagtaac tgcaaagctt tcgttcgtat catcggtttc gacaacgttc gtcaagttca
     5221 atgcatcagt ttcattgcgc acacaccaga atcctactga gtttgagtat tatggcattg
     5281 ggaaaactgt ttttcttgta ccatttgttg tgcttgtaat ttactgtgtt ttttattcgg
     5341 ttttcgctat cgaactgtga aatggaaatg gatggagaag agttaatgaa tgatatggtc
     5401 cttttgttca ttctcaaatt aatattattt gttttttctc ttatttgttg tgtgttgaat
     5461 ttgaaattat aagagatatg caaacatttt gttttgagta aaaatgtgtc aaatcgtggc
     5521 ctctaatgac cgaagttaat atgaggagta aaacacttgt agttgtacca ttatgcttat
     5581 tcactaggca acaaatatat tttcagacct agaaaagctg caaatgttac tgaatacaag
     5641 tatgtcctct tgtgttttag acatttatga actttccttt atgtaatttt ccagaatcct
     5701 tgtcagattc taatcattgc tttataatta tagttatact catggatttg tagttgagta
     5761 tgaaaatatt ttttaatgca ttttatgact tgccaattga ttgacaacat gcatcaatcg
     5821 actatgagta ggcaagatgt tctgtagggg ctatgatgcc tggtcacaac ctatgcatat
     5881 gtcgagctca agaaatctag aaagaagaaa gggaagagaa agaattgtga gcgctcacaa
     5941 ttgaaagact agaaagaaga aagggaagag aaagaattgt gagcgctcac aattgaaaga
     6001 ctagaaagaa gaaagggaag agaaagaatt gtgagcgctc acaattgaaa gactagaaag
     6061 aagaaaggga agagaaagaa ttgtgagcgc tcacaattga aagactagaa agaagaaagg
     6121 gaagagaaag aattgtgagc gctcacaatt gaaagactag aaagaagaaa gggaagagaa
     6181 agaattgtga gcgctcacaa ttgaaagact agtggatcga tcttcgcaag acccttcctc
     6241 tatataagga agttcatttc atttggagag gacgaacaat gaaagccttc acactcgctc
     6301 tcttcttagc tctttccctc tatctcctgc ccaatccagc cactagtggc tcaacaatgg
     6361 tgagcaaggg cgaggagctg ttcaccgggg tggtgcccat cctggtcgag ctggacggcg
     6421 acgtaaacgg ccacaagttc agcgtgtccg gcgagggcga gggcgatgcc acctacggca
     6481 agctgaccct gaagttcatc tgcaccaccg gcaagctgcc cgtgccctgg cccaccctcg
     6541 tgaccaccct gtcctggggc gtgcagtgct tcgcccgcta ccccgaccac atgaagcagc
     6601 acgacttctt caagtccgcc atgcccgaag gctacgtcca ggagcgcacc atcttcttca
     6661 aggacgacgg caactacaag acccgcgccg aggtgaagtt cgagggcgac accctggtga
     6721 accgcatcga gctgaagggc atcgacttca aggaggacgg caacatcctg gggcacaagc
     6781 tggagtacaa ctactttagc gacaacgtct atatcaccgc cgacaagcag aagaacggca
     6841 tcaaggccaa cttcaagatc cgccacaaca tcgaggacgg cggcgtgcag ctcgccgacc
     6901 actaccagca gaacaccccc atcggcgacg gccccgtgct gctgcccgac aaccactacc
     6961 tgagcaccca gtccaagctg agcaaagacc ccaacgagaa gcgcgatcac atggtcctgc
     7021 tggagttcgt gaccgccgcc gggatcactc tcggcatgga cgagctgtac aagtcaggtc
     7081 atgatgagct ttagctgcat ctcgtctctg ttatgcttaa aaagttcaat gtttcgtttc
     7141 atgtaaaact ttggtggttt gtgttttggg gccttgtata atccctgatg aataagtgtt
     7201 ctactatgtt tccgttcctg ttatctcttt ctttctaatg acaagtcgaa cttcttcttt
     7261 atcatcgctt cgtttttatt atctgtgctt cttttgttta atacgcctgc aaagtgactc
     7321 gactctgttt agtgcagttc tgcgaaactt gtaaatagtc caattgttgg cctctagtaa
     7381 tagatgtagc gaaagtgttg agctgttggg ttctaaggat ggcttgaaca tgttaatctt
     7441 ttaggttctg agtatgatga acattcgttg ttgctaagaa atgcctgtaa tgtcccacaa
     7501 atgtagaaaa tggttcgtac ctttgtccaa gcattgatat gtctgatgag aggaaactgc
     7561 aagatactga gcttggttta acgaaggaga ggcagtttct tccttccaaa gcatttcatt
     7621 tgacaatgcc ttgatcatct taagtagagt ttctgttgtg gaaagtttga aactttgaag
     7681 aaacgactct caagtaaatt gatgatcaca agtgaaagtg tatgttacta ggatcctttt
     7741 caaatcagtg cgcaagacgt gacgtaagta tccgagtcag tttttatttt tctactaatt
     7801 tggtcgttta tttcggcgtg taggacatgg caaccgggcc tgaatttcgc gggtattctg
     7861 tttctattcc aactttttct tgatccgcag ccattaacga cttttgaata gatacgctga
     7921 cacgccaagc ctcgctagtc aaaagtgtac caaacaacgc tttacagcaa gaacggaatg
     7981 cgcgtgacgc tcgcggtgac gccatttcgc cttttcagaa atggataaat agccttgctt
     8041 cctattatat cttcccaaat taccaataca ttacactagc atctgaattt cataaccaat
     8101 ctcgatacac caaatcgcca tggcttctat gatatcctct tcagctgtga ctacagtcag
     8161 ccgtgcttct acggtgcaat cggccgcggt ggctccattc ggcggcctca aatccatgac
     8221 tggattccca gttaagaagg tcaacactga cattacttcc attacaagca atggtggaag
     8281 agtaaagtgc atggtgacgg tgttcggcat tctgaatctc accgaggact ccttcttcga
     8341 tgagagccgg cggctagacc ccgccggcgc tgtcaccgcg gcgatcgaaa tgctgcgagt
     8401 cggatcagac gtcgtggatg tcggaccggc cgccagccat ccggacgcga ggcctgtatc
     8461 gccggccgat gagatcagac gtattgcgcc gctcttagac gccctgtccg atcagatgca
     8521 ccgtgtttca atcgacagct tccaaccgga aacccagcgc tatgcgctca agcgcggcgt
     8581 gggctacctg aacgatatcc aaggatttcc tgaccctgcg ctctatcccg atattgctga
     8641 ggcggactgc aggctggtgg ttatgcactc agcgcagcgg gatggcatcg ccacccgcac
     8701 cggtcacctt cgacccgaag acgcgctcga cgagattgtg cggttcttcg aggcgcgggt
     8761 ttccgccttg cgacggagcg gggtcgctgc cgaccggctc atcctcgatc cggggatggg
     8821 atttttcttg agccccgcac cggaaacatc gctgcacgtg ctgtcgaacc ttcaaaagct
     8881 gaagtcggcg ttggggcttc cgctacttgt ctcggtgtcg cggaaatcct tcttgggcgc
     8941 caccgttggc cttcctgtaa aggatctggg tccagcgagc cttgcggcgg aacttcacgc
     9001 gatcggcaat ggcgctgact acgtccgcac ccacgcgcct ggagatctgc gaagcgcaat
     9061 caccatctcg gaaaccctcg cgaaatttcg cagtcgcgac gccagagaca gagggttaga
     9121 tcatgcctag cttggctgca ggtcgacctg caggcatgcc cgctgaaatc accagtctct
     9181 ctctacaaat ctatctctct ctataataat gtgtgagtag ttcccagata agggaattag
     9241 ggttcttata gggtttcgct catgtgttga gcatataaga aacccttagt atgtatttgt
     9301 atttgtaaaa tacttctatc aataaaattt ctaattccta aaaccaaaat ccaggggtac
     9361 cgtattctag accaagcatg cacagctggc gttctcagcc agatcccctt gacaggatat
     9421 attggcgggt aaactaagtc gctgtatgtg tttgtttgag atctcatgtg agcaaaaggc
     9481 cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca taggctccgc
     9541 ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga
     9601 ctataaagat accaggcgtt tccccctgga agctccctcg tgcgctctcc tgttccgacc
     9661 ctgccgctta ccggatacct gtccgccttt ctcccttcgg gaagcgtggc gctttctcat
     9721 agctcacgct gtaggtatct cagttcggtg taggtcgttc gctccaagct gggctgtgtg
     9781 cacgaacccc ccgttcagcc cgaccgctgc gccttatccg gtaactatcg tcttgagtcc
     9841 aacccggtaa gacacgactt atcgccactg gcagcagcca ctggtaacag gattagcaga
     9901 gcgaggtatg taggcggtgc tacagagttc ttgaagtggt ggcctaacta cggctacact
     9961 agaagaacag tatttggtat ctgcgctctg ctgaagccag ttaccttcgg aagaagagtt
    10021 ggtagctctt gatccggcaa acaaaccacc gctggtagcg gtggtttttt tgtttgcaag
    10081 cagcagatta cgcgcagaaa aaaaggatct caagaagatc ctttgatctt ttctacgggg
    10141 tctgacgctc agtggaacga aaactcacgt taagggattt tggtcatgag attatcaaaa
    10201 aggatcttca cctagatcct tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata
    10261 tatgtgtaac attggtctag tgattatttg ccgactacct tggtgatctc gcctttcacg
    10321 tagtgaacaa attcttccaa ctgatctgcg cgcgaggcca agcgatcttc ttgtccaaga
    10381 taagcctgcc tagcttcaag tatgacgggc tgatactggg ccggcaggcg ctccattgcc
    10441 cagtcggcag cgacatcctt cggcgcgatt ttgccggtta ctgcgctgta ccaaatgcgg
    10501 gacaacgtaa gcactacatt tcgctcatcg ccagcccagt cgggcggcga gttccatagc
    10561 gttaaggttt catttagcgc ctcaaataga tcctgttcag gaaccggatc aaagagttcc
    10621 tccgccgctg gacctaccaa ggcaacgcta tgttctcttg cttttgtcag caagatagcc
    10681 agatcaatgt cgatcgtggc tggctcgaag atacctgcaa gaatgtcatt gcgctgccat
    10741 tctccaaatt gcagttcgcg cttagctgga taacgccacg gaatgatgtc gtcgtgcaca
    10801 acaatggtga cttctacagc gcggagaatc tcgctctctc caggggaagc cgaagtttcc
    10861 aaaaggtcgt tgatcaaagc tcgccgcgtt gtttcatcaa gccttacggt caccgtaacc
    10921 agcaaatcaa tatcactgtg tggcttcagg ccgccatcca ctgcggagcc gtacaaatgt
    10981 acggccagca acgtcggttc gagatggcgc tcgatgacgc caactacctc tgatagttga
    11041 gtcgatactt cggcgatcac cgcttccctc ataacacccc ttgtattact gtttatgtaa
    11101 gcagacagtt ttattgttca tgatgatata tttttatctt gtgcaatgta acatcagaga
    11161 ttttgagaca caacgtggct ttgttgaata aatcgaactt ttgctgagtt gaaggatcag
    11221 atcacgcatc ttcccgacaa cgcagaccgt tccgtggcaa agcaaaagtt caaaatcacc
    11281 aactggtcca cctacaacaa agctctcatc aaccgtggct ccctcacttt ctggctggat
    11341 gatggggcga ttcaggcgat ccccatccaa cagcccgccg tcgagcgggc t
//
