LOCUS       Exported                3908 bp ds-DNA     circular UNA 14-OCT-2015
DEFINITION  module A greengate.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      pVL03 (pVL03)
  ORGANISM  pVL03 (pVL03)
REFERENCE   1  (bases 1 to 3908)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Aug 23, 2018 from SnapGene Viewer 4.2.3
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..3908
                     /organism="pVL03 (pVL03)"
                     /mol_type="genomic DNA"
     gene            201..1061
                     /label=Ampicilin
     region          1216..1835
                     /label=pBR322_Origin
     gene            1379
                     /label=responsible for high copy number
                     /label=responsible for high copy number
     promoter        2144..2173
                     /label=Lac_promoter
     primer_bind     2171..2189
                     /label=primer_seq_GG1_for
     gene            2182..2198
                     /label=lac operator
     gene            2208..2209
                     /label=pGEM-T easy [2653-2674]
     primer_bind     2212..2229
                     /label=SP6_primer
     gene            2231..2234
                     /label=auxiliary bases for SP6
     gene            2232..2234
                     /label=pUC19-RC_no-BsaI_noProlac
     misc_feature    2248..3473
                     /label=Insert
                     /note="/feature_type=Insertion"
                     /note="/origin=pLTP1_GG_insert (1,252bp linear)Fragment: 
                     from Eco31I cut at 11^12 to Eco31I cut at 1237^1238, 
                     1,226bpOrientation: originalPlus strand 5': no changePlus 
                     strand 3': no changeMinus strand 5': no changeMinus strand 
                     3': no change"
     promoter        2252..3473
                     /label=pLTP1
     misc_feature    join(3474..3908,1..2247)
                     /label=nonstandard type: Vector
                     /note="/origin=pVL03 (3,749bp circular)Target vector: from 
                     Eco31I cut at 3314^3315 to Eco31I cut at 2247^2248, 
                     2,682bpOrientation: originalPlus strand 5': no changePlus 
                     strand 3': no changeMinus strand 5': no changeMinus strand 
                     3': no change"
     CDS             3474..3760
                     /label=open reading frame#1
     CDS             3474..3476
                     /gene="open reading frame#1"
                     /label=open reading frame#1
     gene            3491..3514
                     /label=pGEM-T easy [2653-2674]
     gene            3491..3494
                     /gene="auxiliary bases for T7 (complement)"
                     /label=auxiliary bases for T7
     promoter        3495..3513
                     /label=T7_promoter
     gene            3523..3666
                     /label=LacZ_a
     primer_bind     3523..3545
                     /label=M13_pUC_fwd_primer
     primer_bind     complement(3533..3550)
                     /label=primer_seq_GG1_rev
     primer_bind     3858..3880
                     /label=pGEX_3_primer
ORIGIN
        1 gacgaaaggg cctcgtgata cgcctatttt tataggttaa tgtcatgata ataatggttt
       61 cttagacgtc aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt
      121 tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat
      181 aatattgaaa aaggaagagt atgagtattc aacatttccg tgtcgccctt attccctttt
      241 ttgcggcatt ttgccttcct gtttttgctc acccagaaac gctggtgaaa gtaaaagatg
      301 ctgaagatca gttgggtgca cgagtgggtt acatcgaact ggatctcaac agcggtaaga
      361 tccttgagag ttttcgcccc gaagaacgtt ttccaatgat gagcactttt aaagttctgc
      421 tatgtggcgc ggtattatcc cgtattgacg ccgggcaaga gcaactcggt cgccgcatac
      481 actattctca gaatgacttg gttgagtact caccagtcac agaaaagcat cttacggatg
      541 gcatgacagt aagagaatta tgcagtgctg ccataaccat gagtgataac actgcggcca
      601 acttacttct gacaacgatc ggaggaccga aggagctaac cgcttttttg cacaacatgg
      661 gggatcatgt aactcgcctt gatcgttggg aaccggagct gaatgaagcc ataccaaacg
      721 acgagcgtga caccacgatg cctgtagcaa tggcaacaac gttgcgcaaa ctattaactg
      781 gcgaactact tactctagct tcccggcaac aattaataga ctggatggag gcggataaag
      841 ttgcaggacc acttctgcgc tcggcccttc cggctggctg gtttattgct gataaatctg
      901 gagccggtga gcgtggctct cgaggtatca ttgcagcact ggggccagat ggtaagccct
      961 cccgtatcgt agttatctac acgacgggga gtcaggcaac tatggatgaa cgaaatagac
     1021 agatcgctga gataggtgcc tcactgatta agcattggta actgtcagac caagtttact
     1081 catatatact ttagattgat ttaaaacttc atttttaatt taaaaggatc taggtgaaga
     1141 tcctttttga taatctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt
     1201 cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct
     1261 gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc
     1321 taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgttc
     1381 ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc
     1441 tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg
     1501 ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt
     1561 cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg
     1621 agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg
     1681 gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt
     1741 atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag
     1801 gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt
     1861 gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgta
     1921 ttaccgcctt tgagtgagct gataccgctc gccgcagccg aacgaccgag cgcagcgagt
     1981 cagtgagcga ggaagcggaa gagcgcccaa tacgcaaacc gcctctcccc gcgcgttggc
     2041 cgattcatta atgcagctgg cacgacaggt ttcccgactg gaaagcgggc agtgagcgca
     2101 acgcaattaa tgtgagttag ctcactcatt aggcacccca ggctttacac tttatgcttc
     2161 cggctcgtat gttgtgtgga attgtgagcg gataacaatt tcacacgagc tatttaggtg
     2221 acactataga agtgaagctt ggtctcaacc tgaccaaaat gattaacttg cattacagtt
     2281 gggaagtatc aagtaaacaa cattttgttt ttgtttgata tcgggaatct caaaaccaaa
     2341 gtccacacta gtttttggac tatataatga taaaagtcag atatctacta atactagttg
     2401 atcagtatat tcgaaaacat gactttccaa atgtaagtta tttacttttt ttttgctatt
     2461 ataattaaga tcaataaaaa tgtctaagtt ttaaatcttt atcattatat ccaaacaatc
     2521 ataatcttat tgttaatctc tcatcaacac acagttttta aaataaatta attacccttt
     2581 gcatgatacc gaagagaaac gaattcgttc aaataatttt ataacaggaa ataaaataga
     2641 taaccgaaat aaagatagaa tgatttctta gtactaactc ttaacaacag ttttatttaa
     2701 atgacttttg taaaaaaaac aaagttaact tatacacgta cacgtgtcga aaatattatt
     2761 gacaatggat agcatgattc ttattagagt catgtaaaag ataaacacat gcaaatatat
     2821 atatgaataa tatgttgtta agataaacta gacgattaga atatatagca catctatagt
     2881 ttgtaaaata actatttctc aactagactt aagtcttcga aatacataaa taaacaaaac
     2941 tataaaaatt cagaaaaaaa catgagagta cgttagtaaa atgtattttt ttggtaaaat
     3001 aatcactttt catcaggtct ttttgtaaag cagttttcat gttagataaa cgagatttta
     3061 atttttttta aaaagaagta aactaactat gttcctatct acacacctat aattttgaaa
     3121 caattacaaa aacaaacaat gaaaatgcaa agaaggacgt aggcactgtc acactacaaa
     3181 tacgttaata aaatgtattt tggtcgaatt ataactttcc atacgataaa gttgaattaa
     3241 catgtcaaac aaaagagatg agtggtccta tacatagtta ggaattagga acctctaaat
     3301 taaatgagta caaccaccaa ctactccttc cctctataat ctatcgcatt cacaccacat
     3361 aacatatacg tacctactct atataacact cactccccaa actctcttca tcatccatca
     3421 ctacacacat ctcctattgc aaacgaacat aaaacactac ctaagagatc aataacatga
     3481 gaccgaattc tcgccctata gtgagtcgta ttacctggcc gtcgttttac aacgtcgtga
     3541 ctgggaaaac cctggcgtta cccaacttaa tcgccttgca gcacatcccc ctttcgccag
     3601 ctggcgtaat agcgaagagg cccgcaccga tcgcccttcc caacagttgc gcagcctgaa
     3661 tggcgaatgg cgcctgatgc ggtattttct ccttacgcat ctgtgcggta tttcacaccg
     3721 catatggtgc actctcagta caatctgctc tgatgccgca tagttaagcc agccccgaca
     3781 cccgccaaca cccgctgacg cgccctgacg ggcttgtctg ctcccggcat ccgcttacag
     3841 acaagctgtg accgtctccg ggagctgcat gtgtcagagg ttttcaccgt catcaccgaa
     3901 acgcgcga
//
