LOCUS       Exported                4961 bp ds-DNA     circular UNA 14-OCT-2015
DEFINITION  module A greengate.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      pVL03 (pVL03)
  ORGANISM  pVL03 (pVL03)
REFERENCE   1  (bases 1 to 4961)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Aug 23, 2018 from SnapGene Viewer 4.2.3
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..4961
                     /organism="pVL03 (pVL03)"
                     /mol_type="genomic DNA"
     gene            201..1061
                     /label=Ampicilin
     region          1216..1835
                     /label=pBR322_Origin
     gene            1379
                     /label=responsible for high copy number
                     /label=responsible for high copy number
     promoter        2144..2173
                     /label=Lac_promoter
     primer_bind     2171..2189
                     /label=primer_seq_GG1_for
     gene            2182..2198
                     /label=lac operator
     gene            2208..2209
                     /label=pGEM-T easy [2653-2674]
     primer_bind     2212..2229
                     /label=SP6_primer
     gene            2231..2234
                     /label=auxiliary bases for SP6
     gene            2232..2234
                     /label=pUC19-RC_no-BsaI_noProlac
     promoter        2248..4526
                     /label=AT2G38380 promoter_insert_GG_insert
     misc_feature    2248..4526
                     /label=Insert
                     /note="/feature_type=Insertion"
                     /note="/origin=AT2G38380_GG_insert_pKG44_1 (2,305bp 
                     linear)Fragment: from Eco31I cut at 11^12 to Eco31I cut at 
                     2290^2291, 2,279bpOrientation: originalPlus strand 5': no 
                     changePlus strand 3': no changeMinus strand 5': no 
                     changeMinus strand 3': no change"
     misc_feature    join(4527..4961,1..2247)
                     /label=nonstandard type: Vector
                     /note="/origin=pVL03 (3,749bp circular)Target vector: from 
                     Eco31I cut at 3314^3315 to Eco31I cut at 2247^2248, 
                     2,682bpOrientation: originalPlus strand 5': no changePlus 
                     strand 3': no changeMinus strand 5': no changeMinus strand 
                     3': no change"
     CDS             4527..4813
                     /label=open reading frame#1
     CDS             4527..4529
                     /gene="open reading frame#1"
                     /label=open reading frame#1
     gene            4544..4567
                     /label=pGEM-T easy [2653-2674]
     gene            4544..4547
                     /gene="auxiliary bases for T7 (complement)"
                     /label=auxiliary bases for T7
     promoter        4548..4566
                     /label=T7_promoter
     gene            4576..4719
                     /label=LacZ_a
     primer_bind     4576..4598
                     /label=M13_pUC_fwd_primer
     primer_bind     complement(4586..4603)
                     /label=primer_seq_GG1_rev
     primer_bind     4911..4933
                     /label=pGEX_3_primer
ORIGIN
        1 gacgaaaggg cctcgtgata cgcctatttt tataggttaa tgtcatgata ataatggttt
       61 cttagacgtc aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt
      121 tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat
      181 aatattgaaa aaggaagagt atgagtattc aacatttccg tgtcgccctt attccctttt
      241 ttgcggcatt ttgccttcct gtttttgctc acccagaaac gctggtgaaa gtaaaagatg
      301 ctgaagatca gttgggtgca cgagtgggtt acatcgaact ggatctcaac agcggtaaga
      361 tccttgagag ttttcgcccc gaagaacgtt ttccaatgat gagcactttt aaagttctgc
      421 tatgtggcgc ggtattatcc cgtattgacg ccgggcaaga gcaactcggt cgccgcatac
      481 actattctca gaatgacttg gttgagtact caccagtcac agaaaagcat cttacggatg
      541 gcatgacagt aagagaatta tgcagtgctg ccataaccat gagtgataac actgcggcca
      601 acttacttct gacaacgatc ggaggaccga aggagctaac cgcttttttg cacaacatgg
      661 gggatcatgt aactcgcctt gatcgttggg aaccggagct gaatgaagcc ataccaaacg
      721 acgagcgtga caccacgatg cctgtagcaa tggcaacaac gttgcgcaaa ctattaactg
      781 gcgaactact tactctagct tcccggcaac aattaataga ctggatggag gcggataaag
      841 ttgcaggacc acttctgcgc tcggcccttc cggctggctg gtttattgct gataaatctg
      901 gagccggtga gcgtggctct cgaggtatca ttgcagcact ggggccagat ggtaagccct
      961 cccgtatcgt agttatctac acgacgggga gtcaggcaac tatggatgaa cgaaatagac
     1021 agatcgctga gataggtgcc tcactgatta agcattggta actgtcagac caagtttact
     1081 catatatact ttagattgat ttaaaacttc atttttaatt taaaaggatc taggtgaaga
     1141 tcctttttga taatctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt
     1201 cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct
     1261 gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc
     1321 taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgttc
     1381 ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc
     1441 tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg
     1501 ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt
     1561 cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg
     1621 agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg
     1681 gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt
     1741 atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag
     1801 gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt
     1861 gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgta
     1921 ttaccgcctt tgagtgagct gataccgctc gccgcagccg aacgaccgag cgcagcgagt
     1981 cagtgagcga ggaagcggaa gagcgcccaa tacgcaaacc gcctctcccc gcgcgttggc
     2041 cgattcatta atgcagctgg cacgacaggt ttcccgactg gaaagcgggc agtgagcgca
     2101 acgcaattaa tgtgagttag ctcactcatt aggcacccca ggctttacac tttatgcttc
     2161 cggctcgtat gttgtgtgga attgtgagcg gataacaatt tcacacgagc tatttaggtg
     2221 acactataga agtgaagctt ggtctcaacc tttgaagcag aagaagacga gtcttcgatg
     2281 gggaagaaga gagtgaagtg aaaaagagag agagagagag attagggagg aaccaaagtc
     2341 ttgtgtttgt gaattgaaac tggtttgata aagacgtgtc ggaacacgcg ccacggtcgt
     2401 ctcgtgaaca gtgttagaaa gtcatgtttt ttttttgtct caatttaaat ttgaaattag
     2461 caaaaatttc gcgtcttttc ccctcttgga tcatataatt gctaatctca attcttaaat
     2521 tgggattaag gaataacctt taaacggcta aaactatctg aatgttttga tattttatca
     2581 gattgttaaa aagtgatcaa aaaaaaacaa agcaatatat ttatcttcac atgcctattc
     2641 aatttaagtg acgtgttgtt atttatcaca attaactgac agaattttgt aaaacgttac
     2701 catgtgactg tttttttttt tttcttatca caaacagaaa attatttccg ttccaatctt
     2761 tcttgccatg gaagaagcta tgttagaaaa tgagctctag cttaccggta atagggaaac
     2821 cagtcttgac aaccatcgag aagagggtga caacgataca tgagcgatgg atagcacata
     2881 agaataaaat gatgaagggt tcaagcaaaa aaaaacttgg taagagtttg aacccaaaac
     2941 atcagatttg tcatctttta ggtgagacaa aaaagtcttg ataacaatgt gtgccggaca
     3001 cagttttttt atctagaaga ttaaactttg atttttctca cctaaaaggt gacaataaag
     3061 tgagctatgt agagtgtcgt caattatttg tccatcttct tgtttgtttt tttaatttct
     3121 tctttttgtc caaccattag actaatattt tcattttgtt tctttctctt ttgtcttcgc
     3181 tttcatcttg ttttcaggaa tgactaatgc attcgaatgt tttttctttt ccttttatgt
     3241 gcattcctac tttatgaaac aattagtttt caattcaatt tcggtgatag ttttagccat
     3301 ttctgtccac tttggttgtc ttagttagtt tattgatgag aagaaaagac aaggagcaaa
     3361 agtattagat taggttaacc aaaaagtcta gcatatggac tcgtatgaaa ttgatatttg
     3421 tatgtattag gtaactttgt tttctcagag attattgagc aaaatgttta taatttatcg
     3481 attagtgaag gtggcttcgg aggctacatt tgcgttattt acgggaaatg gaaggacaaa
     3541 acacaagtgc tttctagttt gaaaataaaa tcaaagaaat attggttaaa atagaacaca
     3601 ctttataatc actgatcaca taatattaca ttattatgac ttatacgatt tacaaaaaca
     3661 attcaggtca aaggcactcg tcgatataac ataaagttgg acattgaaac tgagaatacg
     3721 ttggcgttcg cggatttaac atatattttt ttgtccatat ttttataccg tatcgtctca
     3781 acaattctat catcaacaag aacatgtcac tgataaaaac atgtttatcg cagcatgatg
     3841 atattataca tagacatatc ttacgtttaa catgaatcaa caaccatatc tttagcgacc
     3901 ctacacgaat tcaaatttga tatcaatttc gtacaatttc tatcttacta ctttgaaatt
     3961 tgtgtcgttt ataattattt atcaaactca atgatgcgta ttctattctc agaaggagtc
     4021 atgtatgtct tgaaataaat ctagaaagaa gtataggcga aattgagaac catctcttca
     4081 tagaactata cgtcgattca tagtaagtaa aaagtctaca tctgcaataa aaatgtatgg
     4141 agttagctag ttttgaaaaa tactatatac aattgaagtg aacatgaaaa tcttaattgt
     4201 ttaattaaag cgcgcgaaaa aacacaataa taagttaaat tatgtgcaca taatagatag
     4261 cattgtcaaa aggatcggtt aatttatgat ttcactaatt tttattatcc ttcaaccact
     4321 ctagtaaaat acatattgcc ttaaaacaaa acctactagt cttaaaacga aaaaagaaaa
     4381 aaaaaaaaaa aaaaactcaa tacctaataa tcaaaaatag tactcaagct tccctatata
     4441 atgttccttc ttggctttag ctaattcacc atatacccac actcacaagt cacaactcaa
     4501 ataatctttg tcttcctcct acaaataaca tgagaccgaa ttctcgccct atagtgagtc
     4561 gtattacctg gccgtcgttt tacaacgtcg tgactgggaa aaccctggcg ttacccaact
     4621 taatcgcctt gcagcacatc cccctttcgc cagctggcgt aatagcgaag aggcccgcac
     4681 cgatcgccct tcccaacagt tgcgcagcct gaatggcgaa tggcgcctga tgcggtattt
     4741 tctccttacg catctgtgcg gtatttcaca ccgcatatgg tgcactctca gtacaatctg
     4801 ctctgatgcc gcatagttaa gccagccccg acacccgcca acacccgctg acgcgccctg
     4861 acgggcttgt ctgctcccgg catccgctta cagacaagct gtgaccgtct ccgggagctg
     4921 catgtgtcag aggttttcac cgtcatcacc gaaacgcgcg a
//
