LOCUS       Exported                3235 bp ds-DNA     circular UNA 14-OCT-2015
DEFINITION  Module E GreenGate.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      pVL07 (pVL07)
  ORGANISM  pVL07 (pVL07)
REFERENCE   1  (bases 1 to 3235)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Aug 23, 2018 from SnapGene Viewer 4.2.3
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..3235
                     /organism="pVL07 (pVL07)"
                     /mol_type="genomic DNA"
     gene            201..1061
                     /label=Ampicilin
     region          1216..1835
                     /label=pBR322_origin
     gene            1379
                     /label=responsible for high copy number
     promoter        2144..2173
                     /label=Lac_promoter
     primer_bind     2171..2189
                     /label=primer_seq_GG1_for
     gene            2182..2198
                     /label=lac operator
     gene            2208..2231
                     /label=pGEM-T easy [2653-2674]
     gene            2208..2209
                     /label=pGEM-T easy [2653-2674]
     primer_bind     2212..2229
                     /label=SP6_primer
     misc_feature    2231..2234
                     /label=auxiliary bases for SP6
                     /note="/feature_type=Misc."
     misc_feature    2232..2234
                     /label=pUC19-RC_no-BsaI_noProlac
     misc_feature    2248..2800
                     /label=SCR_term
                     /note="/feature_type=Insertion"
                     /note="/origin=SCR_term_GG_PCR_insert (579bp 
                     linear)Fragment: from Eco31I cut at 11^12 to Eco31I cut at 
                     564^565, 553bpOrientation: originalPlus strand 5': no 
                     changePlus strand 3': no changeMinus strand 5': no 
                     changeMinus strand 3': no change"
     misc_feature    2248..2251
                     /label=overhang_module_E
                     /note="/feature_type=Misc."
     terminator      2252..2800
                     /label=SCR_terminator
     misc_feature    join(2801..3235,1..2247)
                     /label=nonstandard type: Vector
                     /note="/origin=pVL07 (3,749bp circular)Target vector: from 
                     Eco31I cut at 3314^3315 to Eco31I cut at 2247^2248, 
                     2,682bpOrientation: originalPlus strand 5': no changePlus 
                     strand 3': no changeMinus strand 5': no changeMinus strand 
                     3': no change"
     misc_feature    2818..2841
                     /label=pGEM-T easy [2653-2674]
                     /note="/feature_type=Misc."
     gene            2818..2821
                     /label=auxiliary bases for T7
                     /label=auxiliary bases for T7 (complement)
     promoter        2822..2840
                     /label=T7_promoter
     CDS             2842..3087
                     /label=open reading frame#1
     gene            2850..2993
                     /label=LacZ_a
     primer_bind     2850..2872
                     /label=M13_pUC_fwd_primer
     primer_bind     complement(2860..2877)
                     /label=primer_seq_GG1_rev
     gene            3185..3207
                     /label=pGEX-3_primer
ORIGIN
        1 gacgaaaggg cctcgtgata cgcctatttt tataggttaa tgtcatgata ataatggttt
       61 cttagacgtc aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt
      121 tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat
      181 aatattgaaa aaggaagagt atgagtattc aacatttccg tgtcgccctt attccctttt
      241 ttgcggcatt ttgccttcct gtttttgctc acccagaaac gctggtgaaa gtaaaagatg
      301 ctgaagatca gttgggtgca cgagtgggtt acatcgaact ggatctcaac agcggtaaga
      361 tccttgagag ttttcgcccc gaagaacgtt ttccaatgat gagcactttt aaagttctgc
      421 tatgtggcgc ggtattatcc cgtattgacg ccgggcaaga gcaactcggt cgccgcatac
      481 actattctca gaatgacttg gttgagtact caccagtcac agaaaagcat cttacggatg
      541 gcatgacagt aagagaatta tgcagtgctg ccataaccat gagtgataac actgcggcca
      601 acttacttct gacaacgatc ggaggaccga aggagctaac cgcttttttg cacaacatgg
      661 gggatcatgt aactcgcctt gatcgttggg aaccggagct gaatgaagcc ataccaaacg
      721 acgagcgtga caccacgatg cctgtagcaa tggcaacaac gttgcgcaaa ctattaactg
      781 gcgaactact tactctagct tcccggcaac aattaataga ctggatggag gcggataaag
      841 ttgcaggacc acttctgcgc tcggcccttc cggctggctg gtttattgct gataaatctg
      901 gagccggtga gcgtggctct cgaggtatca ttgcagcact ggggccagat ggtaagccct
      961 cccgtatcgt agttatctac acgacgggga gtcaggcaac tatggatgaa cgaaatagac
     1021 agatcgctga gataggtgcc tcactgatta agcattggta actgtcagac caagtttact
     1081 catatatact ttagattgat ttaaaacttc atttttaatt taaaaggatc taggtgaaga
     1141 tcctttttga taatctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt
     1201 cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct
     1261 gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc
     1321 taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgttc
     1381 ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc
     1441 tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg
     1501 ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt
     1561 cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg
     1621 agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg
     1681 gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt
     1741 atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag
     1801 gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt
     1861 gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgta
     1921 ttaccgcctt tgagtgagct gataccgctc gccgcagccg aacgaccgag cgcagcgagt
     1981 cagtgagcga ggaagcggaa gagcgcccaa tacgcaaacc gcctctcccc gcgcgttggc
     2041 cgattcatta atgcagctgg cacgacaggt ttcccgactg gaaagcgggc agtgagcgca
     2101 acgcaattaa tgtgagttag ctcactcatt aggcacccca ggctttacac tttatgcttc
     2161 cggctcgtat gttgtgtgga attgtgagcg gataacaatt tcacacgagc tatttaggtg
     2221 acactataga agtgaagctt ggtctcactg ccagcttgga cgcctcgttc ttagttttct
     2281 tctccttttt cacaaacaat gtgcccataa atagatttac ctgttttttt tttgttatgt
     2341 atcaaaaaga gaggattttt tgaagtcaaa ttgttctcac tcacactctg tattggctac
     2401 ctgattggtg gaatacatag aagttaatca tgaagattag aacacttgat attgaagaaa
     2461 acacttgaaa atggtacatc aataataaaa atgataacac gtttaactga aaaatcattt
     2521 acaaatacac aattcttttg aaggctacca tttttttttt tgggaattgt tatgtatttt
     2581 ttttttagca acttgaattg ttttggttta cttttttcca aaaaaaaaat atatacatgt
     2641 tgttttttat ttttgttatg gcaaagaaat tgagatgttt ggatttttca attaaaaaaa
     2701 aaaaaactca tctacgcctt ttcatatttc gcttgaacaa caacgaaacc ctaatctggt
     2761 taaagttgga ttcggccaag tccaccaccg tggagctctg actatgagac cgaattctcg
     2821 ccctatagtg agtcgtatta cctggccgtc gttttacaac gtcgtgactg ggaaaaccct
     2881 ggcgttaccc aacttaatcg ccttgcagca catccccctt tcgccagctg gcgtaatagc
     2941 gaagaggccc gcaccgatcg cccttcccaa cagttgcgca gcctgaatgg cgaatggcgc
     3001 ctgatgcggt attttctcct tacgcatctg tgcggtattt cacaccgcat atggtgcact
     3061 ctcagtacaa tctgctctga tgccgcatag ttaagccagc cccgacaccc gccaacaccc
     3121 gctgacgcgc cctgacgggc ttgtctgctc ccggcatccg cttacagaca agctgtgacc
     3181 gtctccggga gctgcatgtg tcagaggttt tcaccgtcat caccgaaacg cgcga
//
