LOCUS       Exported                2992 bp ds-DNA     circular UNA 04-NOV-2015
DEFINITION  At4G24550_terminator_PCR_GG primers 84E2,84E3.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      At4G24550_terminator_PCR_GG (At4G24550_terminator_PCR_GG)
  ORGANISM  At4G24550_terminator_PCR_GG (At4G24550_terminator_PCR_GG)
REFERENCE   1  (bases 1 to 2992)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Aug 23, 2018 from SnapGene Viewer 4.2.3
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..2992
                     /organism="At4G24550_terminator_PCR_GG 
                     (At4G24550_terminator_PCR_GG)"
                     /mol_type="genomic DNA"
     misc_feature    1..310
                     /label=nonstandard type: Fragment
                     /note="/origin=At4G24550_term_GG_PCR_insert (336bp 
                     linear)Fragment: from Eco31I cut at 11^12 to Eco31I cut at 
                     321^322, 310bpOrientation: originalPlus strand 5': no 
                     changePlus strand 3': no changeMinus strand 5': no 
                     changeMinus strand 3': no change"
     misc_feature    1..4
                     /label=Module E overhang
                     /note="/feature_type=Misc."
     terminator      5..310
                     /label=At4g24550_term
     misc_feature    311..2992
                     /label=nonstandard type: Fragment
                     /note="/origin=pVL07 (3,749bp circular)Fragment: from 
                     Eco31I cut at 3314^3315 to Eco31I cut at 2247^2248, 
                     2,682bpOrientation: originalPlus strand 5': no changePlus 
                     strand 3': no changeMinus strand 5': no changeMinus strand 
                     3': no change"
     misc_feature    311..314
                     /label=Module E overhang
                     /note="/feature_type=Misc."
     misc_feature    328..351
                     /label=pGEM-T easy [2653-2674]
                     /note="/feature_type=Misc."
     gene            328..331
                     /label=auxiliary bases for T7
                     /label=auxiliary bases for T7 (complement)
     promoter        332..350
                     /label=T7_promoter
     CDS             352..597
                     /label=open reading frame#1
     gene            360..503
                     /label=LacZ_a
     primer_bind     360..382
                     /label=M13_pUC_fwd_primer
     primer_bind     complement(370..387)
                     /label=primer_seq_GG1_rev
     gene            695..717
                     /label=pGEX-3_primer
     gene            946..1806
                     /label=Ampicilin
     region          1961..2580
                     /label=pBR322_origin
     gene            2124
                     /label=responsible for high copy number
     promoter        2889..2918
                     /label=Lac_promoter
     primer_bind     2916..2934
                     /label=primer_seq_GG1_for
     gene            2927..2943
                     /label=lac operator
     gene            2953..2976
                     /label=pGEM-T easy [2653-2674]
     gene            2953..2954
                     /label=pGEM-T easy [2653-2674]
     primer_bind     2957..2974
                     /label=SP6_primer
     misc_feature    2976..2979
                     /label=auxiliary bases for SP6
                     /note="/feature_type=Misc."
     misc_feature    2977..2979
                     /label=pUC19-RC_no-BsaI_noProlac
ORIGIN
        1 ctgccctgtt ttcagataat gtttatcctt cacttgtatg tctaagtact ttctcttttc
       61 attttcggat cacacttgct tcacagtact gtgttgaatg ttgtctcttc tatttggata
      121 ctttcttgtt tcggtatcaa catatatggc tatatgcttt tattttgttt gcccctgaaa
      181 acatcatatg atctcttttc ttggaactgg caaaattgca tcttttggga tttttcttga
      241 atttctgaac tgaactgaat ccaaagattc aaagttaagc aaattgcaaa agaaataaag
      301 gtgcaacgat actatgagac cgaattctcg ccctatagtg agtcgtatta cctggccgtc
      361 gttttacaac gtcgtgactg ggaaaaccct ggcgttaccc aacttaatcg ccttgcagca
      421 catccccctt tcgccagctg gcgtaatagc gaagaggccc gcaccgatcg cccttcccaa
      481 cagttgcgca gcctgaatgg cgaatggcgc ctgatgcggt attttctcct tacgcatctg
      541 tgcggtattt cacaccgcat atggtgcact ctcagtacaa tctgctctga tgccgcatag
      601 ttaagccagc cccgacaccc gccaacaccc gctgacgcgc cctgacgggc ttgtctgctc
      661 ccggcatccg cttacagaca agctgtgacc gtctccggga gctgcatgtg tcagaggttt
      721 tcaccgtcat caccgaaacg cgcgagacga aagggcctcg tgatacgcct atttttatag
      781 gttaatgtca tgataataat ggtttcttag acgtcaggtg gcacttttcg gggaaatgtg
      841 cgcggaaccc ctatttgttt atttttctaa atacattcaa atatgtatcc gctcatgaga
      901 caataaccct gataaatgct tcaataatat tgaaaaagga agagtatgag tattcaacat
      961 ttccgtgtcg cccttattcc cttttttgcg gcattttgcc ttcctgtttt tgctcaccca
     1021 gaaacgctgg tgaaagtaaa agatgctgaa gatcagttgg gtgcacgagt gggttacatc
     1081 gaactggatc tcaacagcgg taagatcctt gagagttttc gccccgaaga acgttttcca
     1141 atgatgagca cttttaaagt tctgctatgt ggcgcggtat tatcccgtat tgacgccggg
     1201 caagagcaac tcggtcgccg catacactat tctcagaatg acttggttga gtactcacca
     1261 gtcacagaaa agcatcttac ggatggcatg acagtaagag aattatgcag tgctgccata
     1321 accatgagtg ataacactgc ggccaactta cttctgacaa cgatcggagg accgaaggag
     1381 ctaaccgctt ttttgcacaa catgggggat catgtaactc gccttgatcg ttgggaaccg
     1441 gagctgaatg aagccatacc aaacgacgag cgtgacacca cgatgcctgt agcaatggca
     1501 acaacgttgc gcaaactatt aactggcgaa ctacttactc tagcttcccg gcaacaatta
     1561 atagactgga tggaggcgga taaagttgca ggaccacttc tgcgctcggc ccttccggct
     1621 ggctggttta ttgctgataa atctggagcc ggtgagcgtg gctctcgagg tatcattgca
     1681 gcactggggc cagatggtaa gccctcccgt atcgtagtta tctacacgac ggggagtcag
     1741 gcaactatgg atgaacgaaa tagacagatc gctgagatag gtgcctcact gattaagcat
     1801 tggtaactgt cagaccaagt ttactcatat atactttaga ttgatttaaa acttcatttt
     1861 taatttaaaa ggatctaggt gaagatcctt tttgataatc tcatgaccaa aatcccttaa
     1921 cgtgagtttt cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga
     1981 gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg
     2041 gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc
     2101 agagcgcaga taccaaatac tgttcttcta gtgtagccgt agttaggcca ccacttcaag
     2161 aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc
     2221 agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg
     2281 cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac
     2341 accgaactga gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga
     2401 aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt
     2461 ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag
     2521 cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg
     2581 gcctttttac ggttcctggc cttttgctgg ccttttgctc acatgttctt tcctgcgtta
     2641 tcccctgatt ctgtggataa ccgtattacc gcctttgagt gagctgatac cgctcgccgc
     2701 agccgaacga ccgagcgcag cgagtcagtg agcgaggaag cggaagagcg cccaatacgc
     2761 aaaccgcctc tccccgcgcg ttggccgatt cattaatgca gctggcacga caggtttccc
     2821 gactggaaag cgggcagtga gcgcaacgca attaatgtga gttagctcac tcattaggca
     2881 ccccaggctt tacactttat gcttccggct cgtatgttgt gtggaattgt gagcggataa
     2941 caatttcaca cgagctattt aggtgacact atagaagtga agcttggtct ca
//
