LOCUS       Exported                3663 bp ds-DNA     circular UNA 14-OCT-2015
DEFINITION  module C GreenGate.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      pVL05 (pVL05)
  ORGANISM  pVL05 (pVL05)
REFERENCE   1  (bases 1 to 3663)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Aug 23, 2018 from SnapGene Viewer 4.2.3
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..3663
                     /organism="pVL05 (pVL05)"
                     /mol_type="genomic DNA"
     gene            201..1061
                     /label=Ampicilin
     misc_feature    1216..1835
                     /label=pBR322_Origin
                     /note="/feature_type=Misc."
     promoter        2144..2173
                     /label=Lac_promoter
     primer_bind     2171..2189
                     /label=primer_seq_GG1_for
     misc_feature    2182..2198
                     /label=Lac_operator
                     /note="/feature_type=Misc."
     misc_feature    2208..2231
                     /label=pGEM-T easy [2653-2674]
                     /note="/feature_type=Misc."
     primer_bind     2212..2229
                     /label=SP6_primer
     misc_feature    2231..2234
                     /label=Auxilary bases for SP6
                     /note="/feature_type=Misc."
     misc_feature    2248..3228
                     /label=Insert
                     /note="/feature_type=Insertion"
                     /note="/origin=VND7_cds (1,007bp linear)Fragment: from 
                     Eco31I cut at 11^12 to Eco31I cut at 992^993, 
                     981bpOrientation: originalPlus strand 5': no changePlus 
                     strand 3': no changeMinus strand 5': no changeMinus strand 
                     3': no change"
     CDS             2254..3228
                     /label=VND7
     misc_feature    join(3229..3663,1..2247)
                     /label=nonstandard type: Vector
                     /note="/origin=pVL05 (3,749bp circular)Target vector: from 
                     Eco31I cut at 3314^3315 to Eco31I cut at 2247^2248, 
                     2,682bpOrientation: originalPlus strand 5': no changePlus 
                     strand 3': no changeMinus strand 5': no changeMinus strand 
                     3': no change"
     misc_feature    3246..3269
                     /label=pGEM-T easy [2653-2674]
                     /note="/feature_type=Misc."
     misc_feature    3246..3249
                     /label=Auxilary bases for T7
                     /note="/feature_type=Misc."
     promoter        3250..3268
                     /label=T7_promoter
     CDS             3270..3515
                     /label=Open reading frame 1
     gene            3278..3421
                     /label=LacZ_a
     primer_bind     3278..3300
                     /label=M13_pUC_fwd_primer
     primer_bind     complement(3288..3305)
                     /label=primer_seq_GG1_rev
     primer_bind     3613..3635
                     /label=pGEX_3_primer
ORIGIN
        1 gacgaaaggg cctcgtgata cgcctatttt tataggttaa tgtcatgata ataatggttt
       61 cttagacgtc aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt
      121 tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat
      181 aatattgaaa aaggaagagt atgagtattc aacatttccg tgtcgccctt attccctttt
      241 ttgcggcatt ttgccttcct gtttttgctc acccagaaac gctggtgaaa gtaaaagatg
      301 ctgaagatca gttgggtgca cgagtgggtt acatcgaact ggatctcaac agcggtaaga
      361 tccttgagag ttttcgcccc gaagaacgtt ttccaatgat gagcactttt aaagttctgc
      421 tatgtggcgc ggtattatcc cgtattgacg ccgggcaaga gcaactcggt cgccgcatac
      481 actattctca gaatgacttg gttgagtact caccagtcac agaaaagcat cttacggatg
      541 gcatgacagt aagagaatta tgcagtgctg ccataaccat gagtgataac actgcggcca
      601 acttacttct gacaacgatc ggaggaccga aggagctaac cgcttttttg cacaacatgg
      661 gggatcatgt aactcgcctt gatcgttggg aaccggagct gaatgaagcc ataccaaacg
      721 acgagcgtga caccacgatg cctgtagcaa tggcaacaac gttgcgcaaa ctattaactg
      781 gcgaactact tactctagct tcccggcaac aattaataga ctggatggag gcggataaag
      841 ttgcaggacc acttctgcgc tcggcccttc cggctggctg gtttattgct gataaatctg
      901 gagccggtga gcgtggctct cgaggtatca ttgcagcact ggggccagat ggtaagccct
      961 cccgtatcgt agttatctac acgacgggga gtcaggcaac tatggatgaa cgaaatagac
     1021 agatcgctga gataggtgcc tcactgatta agcattggta actgtcagac caagtttact
     1081 catatatact ttagattgat ttaaaacttc atttttaatt taaaaggatc taggtgaaga
     1141 tcctttttga taatctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt
     1201 cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct
     1261 gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc
     1321 taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgttc
     1381 ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc
     1441 tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg
     1501 ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt
     1561 cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg
     1621 agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg
     1681 gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt
     1741 atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag
     1801 gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt
     1861 gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgta
     1921 ttaccgcctt tgagtgagct gataccgctc gccgcagccg aacgaccgag cgcagcgagt
     1981 cagtgagcga ggaagcggaa gagcgcccaa tacgcaaacc gcctctcccc gcgcgttggc
     2041 cgattcatta atgcagctgg cacgacaggt ttcccgactg gaaagcgggc agtgagcgca
     2101 acgcaattaa tgtgagttag ctcactcatt aggcacccca ggctttacac tttatgcttc
     2161 cggctcgtat gttgtgtgga attgtgagcg gataacaatt tcacacgagc tatttaggtg
     2221 acactataga agtgaagctt ggtctcaggc tcaatggata atataatgca atcgtcaatg
     2281 ccaccgggat tccgatttca tccgacagag gaagagcttg tgggttatta cctagatagg
     2341 aagatcaatt caatgaagag tgctttagat gtcattgtag agattgatct ctacaaaatg
     2401 gagccatggg atatacaagc gaggtgtaaa ctagggtatg aagagcaaaa cgagtggtac
     2461 ttctttagtc ataaggacag gaagtaccct accgggacta ggaccaaccg agccactgcg
     2521 gctgggttct ggaaagccac gggtagagac aaggcggtac tatcaaaaaa cagtgtcatc
     2581 ggaatgcgga agacacttgt ctactacaag ggtcgagctc ctaatggaag aaagtccgat
     2641 tggatcatgc acgaataccg tctccaaaac tccgagcttg ccccggttca ggaggaaggc
     2701 tgggtggtgt gtcgagcatt taggaagcca attccaaacc agaggccatt agggtacgag
     2761 ccatggcaga accagctcta ccacgtcgaa agtagtaaca actactcatc ttcagtgaca
     2821 atgaacacga gtcatcatat cggtgcatct tcatcaagtc ataaccttaa tcaaatgctc
     2881 atgagcaata accactacaa tcctaataat acatcctcat cgatgcatca atatggcaac
     2941 attgagctcc cgcagttgga cagcccgagc ttgtcgccta gtttagggac gaataaagat
     3001 cagaacgaga gtttcgagca agaagaagag aagagcttta actgtgtgga ttggagaaca
     3061 ctagatacct tgcttgagac acaagtcata catccgcata accctaatat tcttatgttc
     3121 gaaacgcagt cgtataatcc ggcgccaagc ttcccttcca tgcatcaaag ctataatgag
     3181 gtcgaagcta atattcatca ttctcttgga tgcttccctg actcgaaatc agtgagaccg
     3241 aattctcgcc ctatagtgag tcgtattacc tggccgtcgt tttacaacgt cgtgactggg
     3301 aaaaccctgg cgttacccaa cttaatcgcc ttgcagcaca tccccctttc gccagctggc
     3361 gtaatagcga agaggcccgc accgatcgcc cttcccaaca gttgcgcagc ctgaatggcg
     3421 aatggcgcct gatgcggtat tttctcctta cgcatctgtg cggtatttca caccgcatat
     3481 ggtgcactct cagtacaatc tgctctgatg ccgcatagtt aagccagccc cgacacccgc
     3541 caacacccgc tgacgcgccc tgacgggctt gtctgctccc ggcatccgct tacagacaag
     3601 ctgtgaccgt ctccgggagc tgcatgtgtc agaggttttc accgtcatca ccgaaacgcg
     3661 cga
//
