LOCUS       Exported                2925 bp ds-DNA     circular UNA 14-OCT-2015
DEFINITION  module B GreenGate.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      pVL04 (pVL04)
  ORGANISM  pVL04 (pVL04)
REFERENCE   1  (bases 1 to 2925)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Aug 23, 2018 from SnapGene Viewer 4.2.3
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..2925
                     /organism="pVL04 (pVL04)"
                     /mol_type="genomic DNA"
     gene            201..1061
                     /label=Ampicilin
     gene            1216..1835
                     /label=pBR322_Origin
     promoter        2144..2173
                     /label=Lac_promoter
     primer_bind     2171..2189
                     /label=primer_seq_GG1_for
     misc_feature    2182..2198
                     /label=Lac_operator
                     /note="/feature_type=Misc."
     misc_feature    2208..2231
                     /label=pGEM-T easy [2653-2674]
                     /note="/feature_type=Misc."
     primer_bind     2212..2229
                     /label=SP6_primer
     misc_feature    2231..2234
                     /label=Auxiliary bases for SP6
                     /note="/feature_type=Misc."
     misc_feature    2248..2490
                     /label=VP16
                     /note="/feature_type=Insertion"
                     /note="/origin=vp16_f_premix (1,081bp linear)Fragment: from
                     Eco31I cut at 55^56 to Eco31I cut at 293^294, 
                     238bpOrientation: originalPlus strand 5': no changePlus 
                     strand 3': no changeMinus strand 5': no changeMinus strand 
                     3': no change"
     misc_feature    join(2491..2925,1..2247)
                     /label=nonstandard type: Vector
                     /note="/origin=pVL04 (3,749bp circular)Target vector: from 
                     Eco31I cut at 3314^3315 to Eco31I cut at 2247^2248, 
                     2,682bpOrientation: originalPlus strand 5': no changePlus 
                     strand 3': no changeMinus strand 5': no changeMinus strand 
                     3': no change"
     misc_feature    2508..2531
                     /label=pGEM-T easy [2653-2674]
                     /note="/feature_type=Misc."
     misc_feature    2508..2511
                     /label=Auxiliary bases for T7
                     /note="/feature_type=Misc."
     promoter        2512..2530
                     /label=T7_promoter
     CDS             2532..2777
                     /label=Open reading frame1
     gene            2540..2683
                     /label=LacZ_a
     primer_bind     2540..2562
                     /label=M13_pUC_fwd_primer
     primer_bind     complement(2550..2567)
                     /label=primer_seq_GG1_rev
     primer_bind     2875..2897
                     /label=pGEX_3_primer
ORIGIN
        1 gacgaaaggg cctcgtgata cgcctatttt tataggttaa tgtcatgata ataatggttt
       61 cttagacgtc aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt
      121 tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat
      181 aatattgaaa aaggaagagt atgagtattc aacatttccg tgtcgccctt attccctttt
      241 ttgcggcatt ttgccttcct gtttttgctc acccagaaac gctggtgaaa gtaaaagatg
      301 ctgaagatca gttgggtgca cgagtgggtt acatcgaact ggatctcaac agcggtaaga
      361 tccttgagag ttttcgcccc gaagaacgtt ttccaatgat gagcactttt aaagttctgc
      421 tatgtggcgc ggtattatcc cgtattgacg ccgggcaaga gcaactcggt cgccgcatac
      481 actattctca gaatgacttg gttgagtact caccagtcac agaaaagcat cttacggatg
      541 gcatgacagt aagagaatta tgcagtgctg ccataaccat gagtgataac actgcggcca
      601 acttacttct gacaacgatc ggaggaccga aggagctaac cgcttttttg cacaacatgg
      661 gggatcatgt aactcgcctt gatcgttggg aaccggagct gaatgaagcc ataccaaacg
      721 acgagcgtga caccacgatg cctgtagcaa tggcaacaac gttgcgcaaa ctattaactg
      781 gcgaactact tactctagct tcccggcaac aattaataga ctggatggag gcggataaag
      841 ttgcaggacc acttctgcgc tcggcccttc cggctggctg gtttattgct gataaatctg
      901 gagccggtga gcgtggctct cgaggtatca ttgcagcact ggggccagat ggtaagccct
      961 cccgtatcgt agttatctac acgacgggga gtcaggcaac tatggatgaa cgaaatagac
     1021 agatcgctga gataggtgcc tcactgatta agcattggta actgtcagac caagtttact
     1081 catatatact ttagattgat ttaaaacttc atttttaatt taaaaggatc taggtgaaga
     1141 tcctttttga taatctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt
     1201 cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct
     1261 gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc
     1321 taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgttc
     1381 ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc
     1441 tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg
     1501 ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt
     1561 cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg
     1621 agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg
     1681 gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt
     1741 atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag
     1801 gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt
     1861 gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgta
     1921 ttaccgcctt tgagtgagct gataccgctc gccgcagccg aacgaccgag cgcagcgagt
     1981 cagtgagcga ggaagcggaa gagcgcccaa tacgcaaacc gcctctcccc gcgcgttggc
     2041 cgattcatta atgcagctgg cacgacaggt ttcccgactg gaaagcgggc agtgagcgca
     2101 acgcaattaa tgtgagttag ctcactcatt aggcacccca ggctttacac tttatgcttc
     2161 cggctcgtat gttgtgtgga attgtgagcg gataacaatt tcacacgagc tatttaggtg
     2221 acactataga agtgaagctt ggtctcatca gcagcccccc cgaccgatgt cagcctgggg
     2281 gacgagctcc acttagacgg cgaggacgtg gcgatggcgc atgccgacgc gctagacgat
     2341 ttcgatctgg acatgttggg ggacggggat tccccgggtc cgggatttac cccccacgac
     2401 tccgccccct acggcgctct ggatatggcc gacttcgagt ttgagcagat gtttaccgat
     2461 gcccttggaa ttgacgagta cggtgggtaa ctgctgagac cgaattctcg ccctatagtg
     2521 agtcgtatta cctggccgtc gttttacaac gtcgtgactg ggaaaaccct ggcgttaccc
     2581 aacttaatcg ccttgcagca catccccctt tcgccagctg gcgtaatagc gaagaggccc
     2641 gcaccgatcg cccttcccaa cagttgcgca gcctgaatgg cgaatggcgc ctgatgcggt
     2701 attttctcct tacgcatctg tgcggtattt cacaccgcat atggtgcact ctcagtacaa
     2761 tctgctctga tgccgcatag ttaagccagc cccgacaccc gccaacaccc gctgacgcgc
     2821 cctgacgggc ttgtctgctc ccggcatccg cttacagaca agctgtgacc gtctccggga
     2881 gctgcatgtg tcagaggttt tcaccgtcat caccgaaacg cgcga
//
