LOCUS       Exported               11479 bp ds-DNA     circular SYN 29-AUG-2017
DEFINITION  synthetic circular DNA
ACCESSION   urn.local...1367881019035.10
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11479)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Aug 12, 2018 from SnapGene 4.0.8
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..11479
                     /organism="synthetic DNA construct"
     source          4994..5690
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     primer_bind     812..831
                     /label=BII(R)
     repeat_region   887..917
                     /label=5'TR(1)
     misc_feature    887..917
                     /label=31bp spacer
     enhancer        1112..1343
                     /label=core insul enhancer
                     /label=core insul
     enhancer        1406..2132
                     /label=CMV promoter (with TetOs)
     misc_feature    1891
                     /label=A
                     /note="Geneious type: Editing History Replacement"
     TATA_signal     1978..1984
     misc_feature    1994..2033
                     /label=TetR binding sites
                     /note="TetR binding sites"
     misc_feature    1994..2012
                     /label=TetO
                     /note="TetO"
     misc_feature    2015..2033
                     /label=TetO
                     /note="TetO"
     primer_bind     2153..2160
                     /label=primer3
                     /note="Geneious name: Primer"
     misc_feature    2153..2160
                     /label=Kozak
                     /note="Kozak"
     CDS             2161..4989
                     /codon_start=1
                     /label=EGFRvIII
                     /translation="MRPSGTAGAALLALLAALCPASRALEEKKGNYVVTDHGSCVRACG
                     ADSYEMEEDGVRKCKKCEGPCRKVCNGIGIGEFKDSLSINATNIKHFKNCTSISGDLHI
                     LPVAFRGDSFTHTPPLDPQELDILKTVKEITGFLLIQAWPENRTDLHAFENLEIIRGRT
                     KQHGQFSLAVVSLNITSLGLRSLKEISDGDVIISGNKNLCYANTINWKKLFGTSGQKTK
                     IISNRGENSCKATGQVCHALCSPEGCWGPEPRDCVSCRNVSRGRECVDKCNLLEGEPRE
                     FVENSECIQCHPECLPQAMNITCTGRGPDNCIQCAHYIDGPHCVKTCPAGVMGENNTLV
                     WKYADAGHVCHLCHPNCTYGCTGPGLEGCPTNGPKIPSIATGMVGALLLLLVVALGIGL
                     FMRRRHIVRKRTLRRLLQERELVGPLTPSGEAPNQALLRILKETEFKKIKVLGSGAFGT
                     VYKGLWIPEGEKVKIPVAIKELREATSPKANKEILDEAYVMASVDNPHVCRLLGICLTS
                     TVQLITQLMPFGCLLDYVREHKDNIGSQYLLNWCVQIAKGMNYLEDRRLVHRDLAARNV
                     LVKTPQHVKITDFGLAKLLGAEEKEYHAEGGKVPIKWMALESILHRIYTHQSDVWSYGV
                     TVWELMTFGSKPYDGIPASEISSILEKGERLPQPPICTIDVYMIMVKCWMIDADSRPKF
                     RELIIEFSKMARDPQRYLVIQGDERMHLPSPTDSNFYRALMDEEDMDDVVDADEYLIPQ
                     QGFFSSPSTSRTPLLSSLSATSNNSTVACIDRNGLQSCPIKEDSFLQRYSSDPTGALTE
                     DSIDDTFLPVPEYINQSVPKRPAGSVQNPVYHNQPLNPAPSRDPHYQDPHSTAVGNPEY
                     LNTVQPTCVNSTFDSPAHWAQKGSHQISLDNPDYQQDFFPKEAKPNGIFKGSTAENAEY
                     LRVAPQSSEFIGA"
     misc_feature    2179..2250
                     /label=Differ from original sequence
     misc_feature    3427..3429
                     /label=E423G
     misc_feature    4994..5579
                     /label=IRES
     misc_feature    5553
                     /label=G to A
     CDS             5580..5663
                     /codon_start=1
                     /label=NLS
                     /translation="MVSKGGSSDDEATADSQHAAPPKKKRKV"
     CDS             5664..5690
                     /codon_start=1
                     /label=linker
                     /translation="GDPRVPVAT"
     misc_feature    5691..6402
                     /label=Untitled
                     /note="����������������������������������������������������
                     ������������"
     CDS             5691..6401
                     /codon_start=1
                     /label=mCherry
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYKS"
     CDS             6417..6467
                     /codon_start=1
                     /label=SpGFP11
                     /translation="SRDHMVLHEYVNAAGIT"
     polyA_signal    6484..6698
                     /label=BGH polyadenylation sequence
     misc_feature    6772..7097
                     /label=SV40 Promoter
     misc_feature    6776
                     /label=G missing
     CDS             7133..7927
                     /codon_start=1
                     /label=neo
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     misc_feature    7928..7933
                     /label=SalI
                     /note="Geneious type: restriction site"
     misc_feature    7934..8038
                     /label=WPRE
     misc_feature    join(8044..8047,8052,8054..8179)
                     /label=SV40 pA
     enhancer        complement(8428..8658)
                     /label=core insul(1) enhancer
                     /label=core insul(1)
     repeat_region   8933..8967
                     /label=3'TR
     primer_bind     complement(9486..9505)
                     /label=pLac(F)
     CDS             complement(10622..11479)
                     /label=Amp
ORIGIN
        1 actcttcctt tttcaatatt attgaagcat ttatcagggt tattgtctca tgagcggata
       61 catatttgaa tgtatttaga aaaataaaca aataggggtt ccgcgcacat ttccccgaaa
      121 agtgccacct aaattgtaag cgttaatatt ttgttaaaat tcgcgttaaa tttttgttaa
      181 atcagctcat tttttaacca ataggccgaa atcggcaaaa tcccttataa atcaaaagaa
      241 tagaccgaga tagggttgag tgttgttcca gtttggaaca agagtccact attaaagaac
      301 gtggactcca acgtcaaagg gcgaaaaacc gtctatcagg gcgatggccc actacgtgaa
      361 ccatcaccct aatcaagttt tttggggtcg aggtgccgta aagcactaaa tcggaaccct
      421 aaagggagcc cccgatttag agcttgacgg ggaaagccgg cgaacgtggc gagaaaggaa
      481 gggaagaaag cgaaaggagc gggcgctagg gcgctggcaa gtgtagcggt cacgctgcgc
      541 gtaaccacca cacccgccgc gcttaatgcg ccgctacagg gcgcgtccca ttcgccattc
      601 aggctgcgca actgttggga agggcgatcg gtgcgggcct cttcgctatt acgccagctg
      661 gcgaaagggg gatgtgctgc aaggcgatta agttgggtaa cgccagggtt ttcccagtca
      721 cgacgttgta aaacgacggc cagtgagcgc gcctcgttca ttcacgtttt tgaacccgtg
      781 gaggacgggc agactcgcgg tgcaaatgtg ttttacagcg tgatggagca gatgaagatg
      841 ctcgacacgc tgcagaacac gcagctagat taaccctaga aagataatca tattgtgacg
      901 tacgttaaag ataatcatgt gtaaaattga cgcatgtgtt ttatcggtct gtatatcgag
      961 gtttatttat taatttgaat agatattaag ttttattata tttacactta catactaata
     1021 ataaattcaa caaacaattt atttatgttt atttatttat taaaaaaaac aaaaactcaa
     1081 aatttcttct ataaagtaac aaaactttta tgagggacag ccccccccca aagcccccag
     1141 ggatgtaatt acgtccctcc cccgctaggg ggcagcagcg agccgcccgg ggctccgctc
     1201 cggtccggcg ctccccccgc atccccgagc cggcagcgtg cggggacagc ccgggcacgg
     1261 ggaaggtggc acgggatcgc tttcctctga acgcttctcg ctgctctttg agcctgcaga
     1321 cacctggggg gatacgggga aaaggcctcc acggccacta gtaggcgttt tgcgctgctt
     1381 cgcgatgtac gggccagata tacgcgttga cattgattat tgactagtta ttaatagtaa
     1441 tcaattacgg ggtcattagt tcatagccca tatatggagt tccgcgttac ataacttacg
     1501 gtaaatggcc cgcctggctg accgcccaac gacccccgcc cattgacgtc aataatgacg
     1561 tatgttccca tagtaacgcc aatagggact ttccattgac gtcaatgggt ggagtattta
     1621 cggtaaactg cccacttggc agtacatcaa gtgtatcata tgccaagtac gccccctatt
     1681 gacgtcaatg acggtaaatg gcccgcctgg cattatgccc agtacatgac cttatgggac
     1741 tttcctactt ggcagtacat ctacgtatta gtcatcgcta ttaccatggt gatgcggttt
     1801 tggcagtaca tcaatgggcg tggatagcgg tttgactcac ggggatttcc aagtctccac
     1861 cccattgacg tcaatgggag tttgttttgg caccaaaatc aacgggactt tccaaaatgt
     1921 cgtaacaact ccgccccatt gacgcaaatg ggcggtaggc gtgtacggtg ggaggtctat
     1981 ataagcagag ctctccctat cagtgataga gatctcccta tcagtgatag agatcgtcga
     2041 cgagctcgtt tagtgaaccg tcagatcgcc tggagacgcc atccacgctg ttttgacctc
     2101 catagaagac accgggaccg atccagcctc cggactctag cgtttaaact tatcgccacc
     2161 atgcgaccct ccgggacggc cggggcagcg ctcctggcgc tgctggctgc gctctgcccg
     2221 gcgagtcggg ctctggagga aaagaaaggt aattatgtgg tgacagatca cggctcgtgc
     2281 gtccgagcct gtggggccga cagctatgag atggaggaag acggcgtccg caagtgtaag
     2341 aagtgcgaag ggccttgccg caaagtgtgt aacggaatag gtattggtga atttaaagac
     2401 tcactctcca taaatgctac gaatattaaa cacttcaaaa actgcacctc catcagtggc
     2461 gatctccaca tcctgccggt ggcatttagg ggtgactcct tcacacatac tcctcctctg
     2521 gatccacagg aactggatat tctgaaaacc gtaaaggaaa tcacagggtt tttgctgatt
     2581 caggcttggc ctgaaaacag gacggacctc catgcctttg agaacctaga aatcatacgc
     2641 ggcaggacca agcaacatgg tcagttttct cttgcagtcg tcagcctgaa cataacatcc
     2701 ttgggattac gctccctcaa ggagataagt gatggagatg tgataatttc aggaaacaaa
     2761 aatttgtgct atgcaaatac aataaactgg aaaaaactgt ttgggacctc cggtcagaaa
     2821 accaaaatta taagcaacag aggtgaaaac agctgcaagg ccacaggcca ggtctgccat
     2881 gccttgtgct cccccgaggg ctgctggggc ccggagccca gggactgcgt ctcttgccgg
     2941 aatgtcagcc gaggcaggga atgcgtggac aagtgcaacc ttctggaggg tgagccaagg
     3001 gagtttgtgg agaactctga gtgcatacag tgccacccag agtgcctgcc tcaggccatg
     3061 aacatcacct gcacaggacg gggaccagac aactgtatcc agtgtgccca ctacattgac
     3121 ggcccccact gcgtcaagac ctgcccggca ggagtcatgg gagaaaacaa caccctggtc
     3181 tggaagtacg cagacgccgg ccatgtgtgc cacctgtgcc atccaaactg cacctacgga
     3241 tgcactgggc caggtcttga aggctgtcca acgaatgggc ctaagatccc gtccatcgcc
     3301 actgggatgg tgggggccct cctcttgctg ctggtggtgg ccctggggat cggcctcttc
     3361 atgcgaaggc gccacatcgt tcggaagcgc acgctgcgga ggctgctgca ggagagggag
     3421 cttgtggggc ctcttacacc cagtggagaa gctcccaacc aagctctctt gaggatcttg
     3481 aaggaaactg aattcaaaaa gatcaaagtg ctgggctccg gtgcgttcgg cacggtgtat
     3541 aagggactct ggatcccaga aggtgagaaa gttaaaattc ccgtcgctat caaggaatta
     3601 agagaagcaa catctccgaa agccaacaag gaaatcctcg atgaagccta cgtgatggcc
     3661 agcgtggaca acccccacgt gtgccgcctg ctgggcatct gcctcacctc caccgtgcag
     3721 ctcatcacgc agctcatgcc cttcggctgc ctcctggact atgtccggga acacaaagac
     3781 aatattggct cccagtacct gctcaactgg tgtgtgcaga tcgcaaaggg catgaactac
     3841 ttggaggacc gtcgcttggt gcaccgtgac ctggcagcca ggaacgtact ggtgaaaaca
     3901 ccgcagcatg tcaagatcac agattttggg ctggccaaac tgctgggtgc ggaagagaaa
     3961 gaataccatg cagaaggagg caaagtgcct atcaagtgga tggcattgga atcaatttta
     4021 cacagaatct atacccacca gagtgatgtc tggagctacg gggtgaccgt ttgggagttg
     4081 atgacctttg gatccaagcc atatgacgga atccctgcca gcgagatctc ctccatcctg
     4141 gagaaaggag aacgcctccc tcagccaccc atatgtacca tcgatgtcta catgatcatg
     4201 gtcaagtgct ggatgataga cgcagatagt cgcccaaagt tccgtgagtt gatcatcgaa
     4261 ttctccaaaa tggcccgaga cccccagcgc taccttgtca ttcaggggga tgaaagaatg
     4321 catttgccaa gtcctacaga ctccaacttc taccgtgccc tgatggatga agaagacatg
     4381 gacgacgtgg tggatgccga cgagtacctc atcccacagc agggcttctt cagcagcccc
     4441 tccacgtcac ggactcccct cctgagctct ctgagtgcaa ccagcaacaa ttccaccgtg
     4501 gcttgcattg atagaaatgg gctgcaaagc tgtcccatca aggaagacag cttcttgcag
     4561 cgatacagct cagaccccac aggcgccttg actgaggaca gcatagacga caccttcctc
     4621 ccagtgcctg aatacataaa ccagtccgtt cccaaaaggc ccgctggctc tgtgcagaat
     4681 cctgtctatc acaatcagcc tctgaacccc gcgcccagca gagacccaca ctaccaggac
     4741 ccccacagca ctgcagtggg caaccccgag tatctcaaca ctgtccagcc cacctgtgtc
     4801 aacagcacat tcgacagccc tgcccactgg gcccagaaag gcagccacca aattagcctg
     4861 gacaaccctg actaccagca ggacttcttt cccaaggaag ccaagccaaa tggcatcttt
     4921 aagggctcca cagctgaaaa tgcagaatac ctaagggtcg cgccacaaag cagtgaattt
     4981 attggagcat agcccctctc cctccccccc ccctaacgtt actggccgaa gccgcttgga
     5041 ataaggccgg tgtgcgtttg tctatatgtt attttccacc atattgccgt cttttggcaa
     5101 tgtgagggcc cggaaacctg gccctgtctt cttgacgagc attcctaggg gtctttcccc
     5161 tctcgccaaa ggaatgcaag gtctgttgaa tgtcgtgaag gaagcagttc ctctggaagc
     5221 ttcttgaaga caaacaacgt ctgtagcgac cctttgcagg cagcggaacc ccccacctgg
     5281 cgacaggtgc ctctgcggcc aaaagccacg tgtataagat acacctgcaa aggcggcaca
     5341 accccagtgc cacgttgtga gttggatagt tgtggaaaga gtcaaatggc tctcctcaag
     5401 cgtattcaac aaggggctga aggatgccca gaaggtaccc cattgtatgg gatctgatct
     5461 ggggcctcgg tgcacatgct ttacatgtgt ttagtcgagg ttaaaaaaac gtctaggccc
     5521 cccgaaccac ggggacgtgg ttttcctttg aaaaacacga tgataatatg gccacaacca
     5581 tggtctctaa aggaggttcg tccgacgacg aagcaacagc ggactcgcag cacgccgcac
     5641 ctcctaagaa gaaaaggaag gtaggggatc cccgggtacc ggtcgccacc atggtgagca
     5701 agggcgagga ggataacatg gccatcatca aggagttcat gcgcttcaag gtgcacatgg
     5761 agggctccgt gaacggccac gagttcgaga tcgagggcga gggcgagggc cgcccctacg
     5821 agggcaccca gaccgccaag ctgaaggtga ccaagggtgg ccccctgccc ttcgcctggg
     5881 acatcctgtc ccctcagttc atgtacggct ccaaggccta cgtgaagcac cccgccgaca
     5941 ttcccgacta cttgaagctg tccttccccg agggcttcaa gtgggagcgc gtgatgaact
     6001 tcgaggacgg cggcgtggtg accgtgaccc aggactcctc cctgcaggac ggcgagttca
     6061 tctacaaggt gaagctgcgc ggcaccaact tcccctccga cggccccgta atgcagaaga
     6121 agaccatggg ctgggaggcc tcctccgagc ggatgtaccc cgaggacggc gccctgaagg
     6181 gcgagatcaa gcagaggctg aagctgaagg acggcggcca ctacgacgct gaggtcaaga
     6241 ccacctacaa ggccaagaag cccgtgcagc tgcccggcgc ctacaacgtc aacatcaagt
     6301 tggacatcac ctcccacaac gaggactaca ccatcgtgga acagtacgaa cgcgccgagg
     6361 gccgccactc caccggcggc atggacgagc tgtacaagtc ttaactcgag ggaggcagcc
     6421 gggaccacat ggtgctgcac gagtacgtga acgccgccgg catcacatag cgaatttaaa
     6481 tcggcctcga ctgtgccttc tagttgccag ccatctgttg tttgcccctc ccccgtgcct
     6541 tccttgaccc tggaaggtgc cactcccact gtcctttcct aataaaatga ggaaattgca
     6601 tcgcattgtc tgagtaggtg tcattctatt ctggggggtg gggtggggca ggacagcaag
     6661 ggggaggatt gggaagacaa tagcaggcat gctggggacg cggccgcaat ttaacgcgaa
     6721 ttaattctgt ggaatgtgtg tcagttaggg tgtggaaagt ccccaggctc cccaggcagg
     6781 cagaagtatg caaagcatgc atctcaatta gtcagcaacc aggtgtggaa agtccccagg
     6841 ctccccagca ggcagaagta tgcaaagcat gcatctcaat tagtcagcaa ccatagtccc
     6901 gcccctaact ccgcccatcc cgcccctaac tccgcccagt tccgcccatt ctccgcccca
     6961 tggctgacta atttttttta tttatgcaga ggccgaggcc gcctctgcct ctgagctatt
     7021 ccagaagtag tgaggaggct tttttggagg cctaggcttt tgcaaaaagc tcccgggagc
     7081 ttgtatatcc attttcggat ctgatcaaga gacaggatga ggatcgtttc gcatgattga
     7141 acaagatgga ttgcacgcag gttctccggc cgcttgggtg gagaggctat tcggctatga
     7201 ctgggcacaa cagacaatcg gctgctctga tgccgccgtg ttccggctgt cagcgcaggg
     7261 gcgcccggtt ctttttgtca agaccgacct gtccggtgcc ctgaatgaac tgcaggacga
     7321 ggcagcgcgg ctatcgtggc tggccacgac gggcgttcct tgcgcagctg tgctcgacgt
     7381 tgtcactgaa gcgggaaggg actggctgct attgggcgaa gtgccggggc aggatctcct
     7441 gtcatctcac cttgctcctg ccgagaaagt atccatcatg gctgatgcaa tgcggcggct
     7501 gcatacgctt gatccggcta cctgcccatt cgaccaccaa gcgaaacatc gcatcgagcg
     7561 agcacgtact cggatggaag ccggtcttgt cgatcaggat gatctggacg aagagcatca
     7621 ggggctcgcg ccagccgaac tgttcgccag gctcaaggcg cgcatgcccg acggcgagga
     7681 tctcgtcgtg acccatggcg atgcctgctt gccgaatatc atggtggaaa atggccgctt
     7741 ttctggattc atcgactgtg gccggctggg tgtggcggac cgctatcagg acatagcgtt
     7801 ggctacccgt gatattgctg aagagcttgg cggcgaatgg gctgaccgct tcctcgtgct
     7861 ttacggtatc gccgctcccg attcgcagcg catcgccttc tatcgccttc ttgacgagtt
     7921 cttctgagtc gacaatcaac ctctggatta caaaatttgt gaaagattga ctggtattct
     7981 taactatgtt gctcctttta cgctatgtgg atacgctgct ttaatgcctt tgtatcatgc
     8041 gttaactaaa cttgtttatt gcagcttata atggttacaa ataaagcaat agcatcacaa
     8101 atttcacaaa taaagcattt ttttcactgc attctagttg tggtttgtcc aaactcatca
     8161 atgtatctta tcatgtctgg aattgactca aatgatgtca attagtctat cagaagctca
     8221 tctggtctcc cttccggggg acaagacatc cctgtttaat atttaaacag cagtgttccc
     8281 aaactgggtt cttatatccc ttgctctggt caaccaggtt gcagggtttc ctgtcctcac
     8341 aggaacgaag tccctaaaga aacagtggca gccaggttta gccccggaat tgactggatt
     8401 ccttttttag ggcccattgg tatggctttt tccccgtatc cccccaggtg tctgcaggct
     8461 caaagagcag cgagaagcgt tcagaggaaa gcgatcccgt gccaccttcc ccgtgcccgg
     8521 gctgtccccg cacgctgccg gctcggggat gcggggggag cgccggaccg gagcggagcc
     8581 ccgggcggct cgctgctgcc ccctagcggg ggagggacgt aattacatcc ctgggggctt
     8641 tggggggggg ctgtccctga tatctataac aagaaaatat atatataata agttatcacg
     8701 taagtagaac atgaaataac aatataatta tcgtatgagt taaatcttaa aagtcacgta
     8761 aaagataatc atgcgtcatt ttgactcacg cggtcgttat agttcaaaat cagtgacact
     8821 taccgcattg acaagcacgc ctcacgggag ctccaagcgg cgactgagat gtcctaaatg
     8881 cacagcgacg gattcgcgct atttagaaag agagagcaat atttcaagaa tgcatgcgtc
     8941 aattttacgc agactatctt tctagggtta atctagctgc atcaggatca tatcgtcggg
     9001 tcttttttcc ggctcagtca tcgcccaagc tggcgctatc tgggcatcgg ggaggaagaa
     9061 gcccgtgcct tttcccgcga ggttgaagcg gcatggaaag agtttgccga ggatgactgc
     9121 tgctgcattg acgttgagcg aaaacgcacg tttaccatga tgattcggga aggtgtggcc
     9181 atgcacgcct ttaacggtga actgttcgtt caggccacct gggataccag ttcgtcgcgg
     9241 cttttccgga cacagttccg gatggtcagc ccgaagcgca tcagcaaccc gaacaatacc
     9301 ggcgacagcc ggaactgccg tgccggtgtg cagattaatg acagcggtgc ggcgctggga
     9361 tattacgtca gcgaggacgg gtatcctggc tggatgccgc agaaatggac atggataccc
     9421 cgtgagttac ccggcgggcg cgcttggcgt aatcatggtc atagctgttt cctgtgtgaa
     9481 attgttatcc gctcacaatt ccacacaaca tacgagccgg aagcataaag tgtaaagcct
     9541 ggggtgccta atgagtgagc taactcacat taattgcgtt gcgctcactg cccgctttcc
     9601 agtcgggaaa cctgtcgtgc cagctgcatt aatgaatcgg ccaacgcgcg gggagaggcg
     9661 gtttgcgtat tgggcgctct tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc
     9721 ggctgcggcg agcggtatca gctcactcaa aggcggtaat acggttatcc acagaatcag
     9781 gggataacgc aggaaagaac atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa
     9841 aggccgcgtt gctggcgttt ttccataggc tccgcccccc tgacgagcat cacaaaaatc
     9901 gacgctcaag tcagaggtgg cgaaacccga caggactata aagataccag gcgtttcccc
     9961 ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga tacctgtccg
    10021 cctttctccc ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg tatctcagtt
    10081 cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga accccccgtt cagcccgacc
    10141 gctgcgcctt atccggtaac tatcgtcttg agtccaaccc ggtaagacac gacttatcgc
    10201 cactggcagc agccactggt aacaggatta gcagagcgag gtatgtaggc ggtgctacag
    10261 agttcttgaa gtggtggcct aactacggct acactagaag gacagtattt ggtatctgcg
    10321 ctctgctgaa gccagttacc ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa
    10381 ccaccgctgg tagcggtggt ttttttgttt gcaagcagca gattacgcgc agaaaaaaag
    10441 gatctcaaga agatcctttg atcttttcta cggggtctga cgctcagtgg aacgaaaact
    10501 cacgttaagg gattttggtc atgagattat caaaaaggat cttcacctag atccttttaa
    10561 attaaaaatg aagttttaaa tcaatctaaa gtatatatga gtaaacttgg tctgacagtt
    10621 accaatgctt aatcagtgag gcacctatct cagcgatctg tctatttcgt tcatccatag
    10681 ttgcctgact ccccgtcgtg tagataacta cgatacggga gggcttacca tctggcccca
    10741 gtgctgcaat gataccgcga gacccacgct caccggctcc agatttatca gcaataaacc
    10801 agccagccgg aagggccgag cgcagaagtg gtcctgcaac tttatccgcc tccatccagt
    10861 ctattaattg ttgccgggaa gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg
    10921 ttgttgccat tgctacaggc atcgtggtgt cacgctcgtc gtttggtatg gcttcattca
    10981 gctccggttc ccaacgatca aggcgagtta catgatcccc catgttgtgc aaaaaagcgg
    11041 ttagctcctt cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg ttatcactca
    11101 tggttatggc agcactgcat aattctctta ctgtcatgcc atccgtaaga tgcttttctg
    11161 tgactggtga gtactcaacc aagtcattct gagaatagtg tatgcggcga ccgagttgct
    11221 cttgcccggc gtcaatacgg gataataccg cgccacatag cagaacttta aaagtgctca
    11281 tcattggaaa acgttcttcg gggcgaaaac tctcaaggat cttaccgctg ttgagatcca
    11341 gttcgatgta acccactcgt gcacccaact gatcttcagc atcttttact ttcaccagcg
    11401 tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata agggcgacac
    11461 ggaaatgttg aatactcat
//
