LOCUS       Exported                5894 bp ds-DNA     circular SYN 03-JAN-2018
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5894)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Aug 12, 2018 from SnapGene 4.0.8
            http://www.snapgene.com
COMMENT     LOCUS CMV-TRE+H2B+Ceru 6284 bp DNA circular UNA 02-AUG-2012 
            DEFINITION Concatenation of 4 sequences. ACCESSION VERSION KEYWORDS 
            . SOURCE . ORGANISM .
FEATURES             Location/Qualifiers
     source          1..5894
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     source          4859..5581
     polyA_signal    33..56
                     /label=BGH pA
                     /note="BGH pA"
     misc_feature    339..386
                     /label=FRT
                     /note="FRT"
     gene            394..1414
                     /label=Hygromycin
                     /note="Hygromycin"
     polyA_signal    1546..1676
                     /label=SV40 pA
                     /note="SV40 pA"
     rep_origin      2059..2732
                     /direction=RIGHT
                     /label=pUC ori
                     /note="pUC ori"
     CDS             complement(2877..3737)
                     /label=Amp (bla)
                     /note="Amp (bla)"
     promoter        complement(3738..3836)
                     /label=bla promoter
                     /note="bla promoter"
     primer_bind     4024..4043
                     /label=primer3
                     /note="Geneious name: Primer"
     enhancer        4100..4826
                     /label=CMV promoter (with TetOs)
     promoter        4100..4727
                     /label=CMV
                     /note="CMV"
     misc_feature    4585
                     /label=A
                     /note="Geneious type: Editing History Replacement"
     TATA_signal     4672..4678
     misc_feature    4688..4727
                     /label=TetR binding sites
                     /note="TetR binding sites"
     misc_feature    4688..4706
                     /label=TetO
                     /note="TetO"
     misc_feature    4709..4727
                     /label=TetO
                     /note="TetO"
     misc_feature    4851..4858
                     /label=Kozak
                     /note="Kozak"
     primer_bind     4851..4858
                     /label=primer3
                     /note="Geneious name: Primer"
     CDS             4868..5221
                     /codon_start=1
                     /label=N-TEV
                     /note="N-TEV"
                     /translation="GESLFKGPRDYNPISSTICHLTNESDGHTTSLYGIGFGPFIITNK
                     HLFRRNNGTLLVQSLHGVFKVKNTTTLQQHLIDGRDMIIIRMPKDFPPFPQKLKFREPQ
                     REERICLVTTNFQT"
     CDS             5222..5575
                     /codon_start=1
                     /label=C-TEV
                     /note="C-TEV"
                     /translation="KSMSSMVSDTSCTFPSSDGIFWKHWIQTKDGQCGSPLVSTRDGFI
                     VGIHSASNFTNTNNYFTSVPKNFMELLTNQEAQQWVSGWRLNADSVLWGGHKVFMVKPE
                     EPFQPVKEATQLMN"
     CDS             5596..5628
                     /codon_start=1
                     /label=c-myc epitope
                     /note="c-myc epitope"
                     /translation="EQKLISEEDLN"
     misc_feature    5628..5630
                     /label=New Stop
                     /note="New Stop"
     primer_bind     complement(5634..5653)
                     /label=primer3
                     /note="Geneious name: Primer"
     CDS             5641..5658
                     /label=Polyhistidine tag
                     /note="Polyhistidine tag"
     misc_feature    5659..5661
                     /label=cya
                     /note="STOP (missing now)"
     polyA_signal    5680..5894
                     /label=BGH polyadenylation sequence
                     /note="BGH polyadenylation sequence"
ORIGIN
        1 tgcggtgggc tctatggaga tctatcgatg ttctggggat gcggtgggct ctatggcttc
       61 tgaggcggaa agaaccagct ggggctctag ggggtatccc cacgcgccct gtagcggcgc
      121 attaagcgcg gcgggtgtgg tggttacgcg cagcgtgacc gctacacttg ccagcgccct
      181 agcgcccgct cctttcgctt tcttcccttc ctttctcgcc acgttcgccg gctttccccg
      241 tcaagctcta aatcgggggt ccctttaggg ttccgattta gtgctttacg gcacctcgac
      301 cccaaaaaac ttgattaggg tgatggttca cgtacctaga agttcctatt ccgaagttcc
      361 tattctctag aaagtatagg aacttccttg gccaaaaagc ctgaactcac cgcgacgtct
      421 gtcgagaagt ttctgatcga aaagttcgac agcgtctccg acctgatgca gctctcggag
      481 ggcgaagaat ctcgtgcttt cagcttcgat gtaggagggc gtggatatgt cctgcgggta
      541 aatagctgcg ccgatggttt ctacaaagat cgttatgttt atcggcactt tgcatcggcc
      601 gcgctcccga ttccggaagt gcttgacatt ggggaattca gcgagagcct gacctattgc
      661 atctcccgcc gtgcacaggg tgtcacgttg caagacctgc ctgaaaccga actgcccgct
      721 gttctgcagc cggtcgcgga ggccatggat gcgatcgctg cggccgatct tagccagacg
      781 agcgggttcg gcccattcgg accgcaagga atcggtcaat acactacatg gcgtgatttc
      841 atatgcgcga ttgctgatcc ccatgtgtat cactggcaaa ctgtgatgga cgacaccgtc
      901 agtgcgtccg tcgcgcaggc tctcgatgag ctgatgcttt gggccgagga ctgccccgaa
      961 gtccggcacc tcgtgcacgc ggatttcggc tccaacaatg tcctgacgga caatggccgc
     1021 ataacagcgg tcattgactg gagcgaggcg atgttcgggg attcccaata cgaggtcgcc
     1081 aacatcttct tctggaggcc gtggttggct tgtatggagc agcagacgcg ctacttcgag
     1141 cggaggcatc cggagcttgc aggatcgccg cggctccggg cgtatatgct ccgcattggt
     1201 cttgaccaac tctatcagag cttggttgac ggcaatttcg atgatgcagc ttgggcgcag
     1261 ggtcgatgcg acgcaatcgt ccgatccgga gccgggactg tcgggcgtac acaaatcgcc
     1321 cgcagaagcg cggccgtctg gaccgatggc tgtgtagaag tactcgccga tagtggaaac
     1381 cgacgcccca gcactcgtcc gagggcaaag gaatagcacg tactacgaga tttcgattcc
     1441 accgccgcct tctatgaaag gttgggcttc ggaatcgttt tccgggacgc cggctggatg
     1501 atcctccagc gcggggatct catgctggag ttcttcgccc accccaactt gtttattgca
     1561 gcttataatg gttacaaata aagcaatagc atcacaaatt tcacaaataa agcatttttt
     1621 tcactgcatt ctagttgtgg tttgtccaaa ctcatcaatg tatcttatca tgtctgtata
     1681 ccgtcgacct ctagctagag cttggcgtaa tcatggtcat agctgtttcc tgtgtgaaat
     1741 tgttatccgc tcacaattcc acacaacata cgagccggaa gcataaagtg taaagcctgg
     1801 ggtgcctaat gagtgagcta actcacatta attgcgttgc gctcactgcc cgctttccag
     1861 tcgggaaacc tgtcgtgcca gctgcattaa tgaatcggcc aacgcgcggg gagaggcggt
     1921 ttgcgtattg ggcgctcttc cgcttcctcg ctcactgact cgctgcgctc ggtcgttcgg
     1981 ctgcggcgag cggtatcagc tcactcaaag gcggtaatac ggttatccac agaatcaggg
     2041 gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag
     2101 gccgcgttgc tggcgttttt ccataggctc cgcccccctg acgagcatca caaaaatcga
     2161 cgctcaagtc agaggtggcg aaacccgaca ggactataaa gataccaggc gtttccccct
     2221 ggaagctccc tcgtgcgctc tcctgttccg accctgccgc ttaccggata cctgtccgcc
     2281 tttctccctt cgggaagcgt ggcgctttct catagctcac gctgtaggta tctcagttcg
     2341 gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac cccccgttca gcccgaccgc
     2401 tgcgccttat ccggtaacta tcgtcttgag tccaacccgg taagacacga cttatcgcca
     2461 ctggcagcag ccactggtaa caggattagc agagcgaggt atgtaggcgg tgctacagag
     2521 ttcttgaagt ggtggcctaa ctacggctac actagaagga cagtatttgg tatctgcgct
     2581 ctgctgaagc cagttacctt cggaaaaaga gttggtagct cttgatccgg caaacaaacc
     2641 accgctggta gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga
     2701 tctcaagaag atcctttgat cttttctacg gggtctgacg ctcagtggaa cgaaaactca
     2761 cgttaaggga ttttggtcat gagattatca aaaaggatct tcacctagat ccttttaaat
     2821 taaaaatgaa gttttaaatc aatctaaagt atatatgagt aaacttggtc tgacagttac
     2881 caatgcttaa tcagtgaggc acctatctca gcgatctgtc tatttcgttc atccatagtt
     2941 gcctgactcc ccgtcgtgta gataactacg atacgggagg gcttaccatc tggccccagt
     3001 gctgcaatga taccgcgaga cccacgctca ccggctccag atttatcagc aataaaccag
     3061 ccagccggaa gggccgagcg cagaagtggt cctgcaactt tatccgcctc catccagtct
     3121 attaattgtt gccgggaagc tagagtaagt agttcgccag ttaatagttt gcgcaacgtt
     3181 gttgccattg ctacaggcat cgtggtgtca cgctcgtcgt ttggtatggc ttcattcagc
     3241 tccggttccc aacgatcaag gcgagttaca tgatccccca tgttgtgcaa aaaagcggtt
     3301 agctccttcg gtcctccgat cgttgtcaga agtaagttgg ccgcagtgtt atcactcatg
     3361 gttatggcag cactgcataa ttctcttact gtcatgccat ccgtaagatg cttttctgtg
     3421 actggtgagt actcaaccaa gtcattctga gaatagtgta tgcggcgacc gagttgctct
     3481 tgcccggcgt caatacggga taataccgcg ccacatagca gaactttaaa agtgctcatc
     3541 attggaaaac gttcttcggg gcgaaaactc tcaaggatct taccgctgtt gagatccagt
     3601 tcgatgtaac ccactcgtgc acccaactga tcttcagcat cttttacttt caccagcgtt
     3661 tctgggtgag caaaaacagg aaggcaaaat gccgcaaaaa agggaataag ggcgacacgg
     3721 aaatgttgaa tactcatact cttccttttt caatattatt gaagcattta tcagggttat
     3781 tgtctcatga gcggatacat atttgaatgt atttagaaaa ataaacaaat aggggttccg
     3841 cgcacatttc cccgaaaagt gccacctgac gtcgacggat cgggagatct cccgatcccc
     3901 tatggtgcac tctcagtaca atctgctctg atgccgcata gttaagccag tatctgctcc
     3961 ctgcttgtgt gttggaggtc gctgagtagt gcgcgagcaa aatttaagct acaacaaggc
     4021 aaggcttgac cgacaattgc atgaagaatc tgcttagggt taggcgtttt gcgctgcttc
     4081 gtacgggcca gatatacgcg ttgacattga ttattgacta gttattaata gtaatcaatt
     4141 acggggtcat tagttcatag cccatatatg gagttccgcg ttacataact tacggtaaat
     4201 ggcccgcctg gctgaccgcc caacgacccc cgcccattga cgtcaataat gacgtatgtt
     4261 cccatagtaa cgccaatagg gactttccat tgacgtcaat gggtggagta tttacggtaa
     4321 actgcccact tggcagtaca tcaagtgtat catatgccaa gtacgccccc tattgacgtc
     4381 aatgacggta aatggcccgc ctggcattat gcccagtaca tgaccttatg ggactttcct
     4441 acttggcagt acatctacgt attagtcatc gctattacca tggtgatgcg gttttggcag
     4501 tacatcaatg ggcgtggata gcggtttgac tcacggggat ttccaagtct ccaccccatt
     4561 gacgtcaatg ggagtttgtt ttggcaccaa aatcaacggg actttccaaa atgtcgtaac
     4621 aactccgccc cattgacgca aatgggcggt aggcgtgtac ggtgggaggt ctatataagc
     4681 agagctctcc ctatcagtga tagagatctc cctatcagtg atagagatcg tcgacgagct
     4741 cgtttagtga accgtcagat cgcctggaga cgccatccac gctgttttga cctccataga
     4801 agacaccggg accgatccag cctccggact ctagcgttta aacttaagct tcgccaccat
     4861 gggatccggg gagagtttgt ttaaggggcc aagggactat aacccaatat catccactat
     4921 ttgccacctc actaacgaga gcgatggaca tacaacctct ctctacggga taggcttcgg
     4981 tcctttcatc attaccaata agcatctgtt tcgccgaaac aacggtactc ttctggttca
     5041 atctcttcat ggtgtcttca aggtgaaaaa caccactacg cttcagcaac acctgattga
     5101 tggtagggat atgataatta tcagaatgcc gaaagatttc ccaccttttc cacagaagct
     5161 gaaattcagg gaaccgcaga gagaggagag gatttgtttg gtaacgacca acttccagac
     5221 gaagagtatg agttctatgg tgtccgacac tagctgcacg ttcccctcaa gtgatgggat
     5281 attctggaaa cactggatac agactaaaga cggacagtgt ggaagcccat tggtttccac
     5341 ccgagatggt tttatcgtgg gtatccatag cgcctctaat ttcacaaaca cgaacaacta
     5401 tttcacttca gtgcccaaaa actttatgga gctgctcaca aaccaagaag cgcagcagtg
     5461 ggtaagcggt tggagactta acgctgactc agttctctgg ggggggcaca aagtattcat
     5521 ggtaaagcca gaggagccat tccaaccagt caaagaagcc acacaactta tgaacagcta
     5581 gctcgaggag ggcccgaaca aaaactcatc tcagaagagg atctgaatag cgccgtcgac
     5641 catcatcatc atcatcattg agtttaaacc cgctgatcag cctcgactgt gccttctagt
     5701 tgccagccat ctgttgtttg cccctccccc gtgccttcct tgaccctgga aggtgccact
     5761 cccactgtcc tttcctaata aaatgaggaa attgcatcgc attgtctgag taggtgtcat
     5821 tctattctgg ggggtggggt ggggcaggac agcaaggggg aggattggga agacaatagc
     5881 aggcatgctg ggga
//
