LOCUS       Exported                6302 bp ds-DNA     circular UNA 09-SEP-2016
DEFINITION  Concatenation of 4 sequences.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 6302)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Aug 12, 2018 from SnapGene 4.0.8
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..6302
                     /organism="unspecified"
     source          5564..5590
     source          5594..5614
     polyA_signal    33..56
                     /label=BGH pA
                     /note="BGH pA"
     misc_feature    339..386
                     /label=FRT
                     /note="FRT"
     gene            394..1414
                     /label=Hygromycin
                     /note="Hygromycin"
     polyA_signal    1546..1676
                     /label=SV40 pA
                     /note="SV40 pA"
     rep_origin      2059..2732
                     /label=pUC ori
                     /note="pUC ori"
     CDS             complement(2877..3737)
                     /label=Amp (bla)
                     /note="Amp (bla)"
     promoter        complement(3738..3836)
                     /label=bla promoter
                     /note="bla promoter"
     enhancer        4100..4826
     promoter        4100..4727
                     /label=CMV
                     /note="CMV"
     TATA_signal     4672..4678
     misc_feature    4688..4727
                     /label=TetR binding sites
                     /note="TetR binding sites"
     misc_feature    4688..4706
                     /label=TetO
                     /note="TetO"
     misc_feature    4709..4727
                     /label=TetO
                     /note="TetO"
     misc_feature    4851..4858
                     /label=Kozak
                     /note="Kozak"
     CDS             4868..5221
                     /codon_start=1
                     /label=N-TEV
                     /note="N-TEV"
                     /translation="GESLFKGPRDYNPISSTICHLTNESDGHTTSLYGIGFGPFIITNK
                     HLFRRNNGTLLVQSLHGVFKVKNTTTLQQHLIDGRDMIIIRMPKDFPPFPQKLKFREPQ
                     REERICLVTTNFQT"
     CDS             5240..5260
                     /codon_start=1
                     /label=TVMVsite
                     /note="TVMVsite"
                     /translation="ETVRFQS"
     CDS             5261..5290
                     /codon_start=1
                     /label=HCVs
                     /note="HCVs"
                     /translation="DEMEECSQHL"
     CDS             5306..5392
                     /codon_start=1
                     /label=NZp
                     /translation="ALKKELQANKKELAQLKWELQALKKELAQ"
     CDS             5399..5455
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             5459..5548
                     /codon_start=1
                     /label=CZp
                     /translation="EQLEKKLQALEKKLAQLEWKNQALEKKLAQ"
     CDS             5564..5593
                     /codon_start=1
                     /label=HCVs
                     /note="HCVs"
                     /translation="DEMEECSQHL"
     CDS             5594..5614
                     /codon_start=1
                     /label=TVMVsite
                     /note="TVMVsite"
                     /translation="ETVRFQS"
     CDS             5630..5983
                     /codon_start=1
                     /label=C-TEV
                     /note="C-TEV"
                     /translation="KSMSSMVSDTSCTFPSSDGIFWKHWIQTKDGQCGSPLVSTRDGFI
                     VGIHSASNFTNTNNYFTSVPKNFMELLTNQEAQQWVSGWRLNADSVLWGGHKVFMVKPE
                     EPFQPVKEATQLMN"
     CDS             6004..6036
                     /codon_start=1
                     /label=c-myc epitope
                     /note="c-myc epitope"
                     /translation="EQKLISEEDLN"
     misc_feature    6036..6038
                     /label=New Stop
                     /note="New Stop"
     CDS             6049..6066
                     /label=Polyhistidine tag
                     /note="Polyhistidine tag"
     misc_feature    6067..6069
                     /label=STOP (missing now)
                     /note="STOP (missing now)"
     polyA_signal    6088..6302
                     /label=BGH polyadenylation sequence
                     /note="BGH polyadenylation sequence"
ORIGIN
        1 tgcggtgggc tctatggaga tctatcgatg ttctggggat gcggtgggct ctatggcttc
       61 tgaggcggaa agaaccagct ggggctctag ggggtatccc cacgcgccct gtagcggcgc
      121 attaagcgcg gcgggtgtgg tggttacgcg cagcgtgacc gctacacttg ccagcgccct
      181 agcgcccgct cctttcgctt tcttcccttc ctttctcgcc acgttcgccg gctttccccg
      241 tcaagctcta aatcgggggt ccctttaggg ttccgattta gtgctttacg gcacctcgac
      301 cccaaaaaac ttgattaggg tgatggttca cgtacctaga agttcctatt ccgaagttcc
      361 tattctctag aaagtatagg aacttccttg gccaaaaagc ctgaactcac cgcgacgtct
      421 gtcgagaagt ttctgatcga aaagttcgac agcgtctccg acctgatgca gctctcggag
      481 ggcgaagaat ctcgtgcttt cagcttcgat gtaggagggc gtggatatgt cctgcgggta
      541 aatagctgcg ccgatggttt ctacaaagat cgttatgttt atcggcactt tgcatcggcc
      601 gcgctcccga ttccggaagt gcttgacatt ggggaattca gcgagagcct gacctattgc
      661 atctcccgcc gtgcacaggg tgtcacgttg caagacctgc ctgaaaccga actgcccgct
      721 gttctgcagc cggtcgcgga ggccatggat gcgatcgctg cggccgatct tagccagacg
      781 agcgggttcg gcccattcgg accgcaagga atcggtcaat acactacatg gcgtgatttc
      841 atatgcgcga ttgctgatcc ccatgtgtat cactggcaaa ctgtgatgga cgacaccgtc
      901 agtgcgtccg tcgcgcaggc tctcgatgag ctgatgcttt gggccgagga ctgccccgaa
      961 gtccggcacc tcgtgcacgc ggatttcggc tccaacaatg tcctgacgga caatggccgc
     1021 ataacagcgg tcattgactg gagcgaggcg atgttcgggg attcccaata cgaggtcgcc
     1081 aacatcttct tctggaggcc gtggttggct tgtatggagc agcagacgcg ctacttcgag
     1141 cggaggcatc cggagcttgc aggatcgccg cggctccggg cgtatatgct ccgcattggt
     1201 cttgaccaac tctatcagag cttggttgac ggcaatttcg atgatgcagc ttgggcgcag
     1261 ggtcgatgcg acgcaatcgt ccgatccgga gccgggactg tcgggcgtac acaaatcgcc
     1321 cgcagaagcg cggccgtctg gaccgatggc tgtgtagaag tactcgccga tagtggaaac
     1381 cgacgcccca gcactcgtcc gagggcaaag gaatagcacg tactacgaga tttcgattcc
     1441 accgccgcct tctatgaaag gttgggcttc ggaatcgttt tccgggacgc cggctggatg
     1501 atcctccagc gcggggatct catgctggag ttcttcgccc accccaactt gtttattgca
     1561 gcttataatg gttacaaata aagcaatagc atcacaaatt tcacaaataa agcatttttt
     1621 tcactgcatt ctagttgtgg tttgtccaaa ctcatcaatg tatcttatca tgtctgtata
     1681 ccgtcgacct ctagctagag cttggcgtaa tcatggtcat agctgtttcc tgtgtgaaat
     1741 tgttatccgc tcacaattcc acacaacata cgagccggaa gcataaagtg taaagcctgg
     1801 ggtgcctaat gagtgagcta actcacatta attgcgttgc gctcactgcc cgctttccag
     1861 tcgggaaacc tgtcgtgcca gctgcattaa tgaatcggcc aacgcgcggg gagaggcggt
     1921 ttgcgtattg ggcgctcttc cgcttcctcg ctcactgact cgctgcgctc ggtcgttcgg
     1981 ctgcggcgag cggtatcagc tcactcaaag gcggtaatac ggttatccac agaatcaggg
     2041 gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag
     2101 gccgcgttgc tggcgttttt ccataggctc cgcccccctg acgagcatca caaaaatcga
     2161 cgctcaagtc agaggtggcg aaacccgaca ggactataaa gataccaggc gtttccccct
     2221 ggaagctccc tcgtgcgctc tcctgttccg accctgccgc ttaccggata cctgtccgcc
     2281 tttctccctt cgggaagcgt ggcgctttct catagctcac gctgtaggta tctcagttcg
     2341 gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac cccccgttca gcccgaccgc
     2401 tgcgccttat ccggtaacta tcgtcttgag tccaacccgg taagacacga cttatcgcca
     2461 ctggcagcag ccactggtaa caggattagc agagcgaggt atgtaggcgg tgctacagag
     2521 ttcttgaagt ggtggcctaa ctacggctac actagaagga cagtatttgg tatctgcgct
     2581 ctgctgaagc cagttacctt cggaaaaaga gttggtagct cttgatccgg caaacaaacc
     2641 accgctggta gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga
     2701 tctcaagaag atcctttgat cttttctacg gggtctgacg ctcagtggaa cgaaaactca
     2761 cgttaaggga ttttggtcat gagattatca aaaaggatct tcacctagat ccttttaaat
     2821 taaaaatgaa gttttaaatc aatctaaagt atatatgagt aaacttggtc tgacagttac
     2881 caatgcttaa tcagtgaggc acctatctca gcgatctgtc tatttcgttc atccatagtt
     2941 gcctgactcc ccgtcgtgta gataactacg atacgggagg gcttaccatc tggccccagt
     3001 gctgcaatga taccgcgaga cccacgctca ccggctccag atttatcagc aataaaccag
     3061 ccagccggaa gggccgagcg cagaagtggt cctgcaactt tatccgcctc catccagtct
     3121 attaattgtt gccgggaagc tagagtaagt agttcgccag ttaatagttt gcgcaacgtt
     3181 gttgccattg ctacaggcat cgtggtgtca cgctcgtcgt ttggtatggc ttcattcagc
     3241 tccggttccc aacgatcaag gcgagttaca tgatccccca tgttgtgcaa aaaagcggtt
     3301 agctccttcg gtcctccgat cgttgtcaga agtaagttgg ccgcagtgtt atcactcatg
     3361 gttatggcag cactgcataa ttctcttact gtcatgccat ccgtaagatg cttttctgtg
     3421 actggtgagt actcaaccaa gtcattctga gaatagtgta tgcggcgacc gagttgctct
     3481 tgcccggcgt caatacggga taataccgcg ccacatagca gaactttaaa agtgctcatc
     3541 attggaaaac gttcttcggg gcgaaaactc tcaaggatct taccgctgtt gagatccagt
     3601 tcgatgtaac ccactcgtgc acccaactga tcttcagcat cttttacttt caccagcgtt
     3661 tctgggtgag caaaaacagg aaggcaaaat gccgcaaaaa agggaataag ggcgacacgg
     3721 aaatgttgaa tactcatact cttccttttt caatattatt gaagcattta tcagggttat
     3781 tgtctcatga gcggatacat atttgaatgt atttagaaaa ataaacaaat aggggttccg
     3841 cgcacatttc cccgaaaagt gccacctgac gtcgacggat cgggagatct cccgatcccc
     3901 tatggtgcac tctcagtaca atctgctctg atgccgcata gttaagccag tatctgctcc
     3961 ctgcttgtgt gttggaggtc gctgagtagt gcgcgagcaa aatttaagct acaacaaggc
     4021 aaggcttgac cgacaattgc atgaagaatc tgcttagggt taggcgtttt gcgctgcttc
     4081 gtacgggcca gatatacgcg ttgacattga ttattgacta gttattaata gtaatcaatt
     4141 acggggtcat tagttcatag cccatatatg gagttccgcg ttacataact tacggtaaat
     4201 ggcccgcctg gctgaccgcc caacgacccc cgcccattga cgtcaataat gacgtatgtt
     4261 cccatagtaa cgccaatagg gactttccat tgacgtcaat gggtggagta tttacggtaa
     4321 actgcccact tggcagtaca tcaagtgtat catatgccaa gtacgccccc tattgacgtc
     4381 aatgacggta aatggcccgc ctggcattat gcccagtaca tgaccttatg ggactttcct
     4441 acttggcagt acatctacgt attagtcatc gctattacca tggtgatgcg gttttggcag
     4501 tacatcaatg ggcgtggata gcggtttgac tcacggggat ttccaagtct ccaccccatt
     4561 gacgtcaatg ggagtttgtt ttggcaccaa aatcaacggg actttccaaa atgtcgtaac
     4621 aactccgccc cattgacgca aatgggcggt aggcgtgtac ggtgggaggt ctatataagc
     4681 agagctctcc ctatcagtga tagagatctc cctatcagtg atagagatcg tcgacgagct
     4741 cgtttagtga accgtcagat cgcctggaga cgccatccac gctgttttga cctccataga
     4801 agacaccggg accgatccag cctccggact ctagcgttta aacttaagct tcgccaccat
     4861 gggatccggg gagagtttgt ttaaggggcc aagggactat aacccaatat catccactat
     4921 ttgccacctc actaacgaga gcgatggaca tacaacctct ctctacggga taggcttcgg
     4981 tcctttcatc attaccaata agcatctgtt tcgccgaaac aacggtactc ttctggttca
     5041 atctcttcat ggtgtcttca aggtgaaaaa caccactacg cttcagcaac acctgattga
     5101 tggtagggat atgataatta tcagaatgcc gaaagatttc ccaccttttc cacagaagct
     5161 gaaattcagg gaaccgcaga gagaggagag gatttgtttg gtaacgacca acttccagac
     5221 gggctctggc agcagcgcgg agacggttag attccagagt gacgagatgg aggagtgtag
     5281 tcagcacctt ggaggcggtg gatccgcgct taagaaggaa cttcaagcaa acaaaaaaga
     5341 gttggcccaa ctgaagtggg agttgcaggc gctcaaaaag gagctcgccc agaccggtgc
     5401 tactaatttc agtctcctca aacaagcagg tgatgtggaa gagaatcccg gccccgggga
     5461 gcaactcgaa aagaagctgc aagcgttgga gaagaagctc gcacaacttg aatggaaaaa
     5521 tcaggcactg gaaaagaaac tggctcaagg tggcggagga tctgatgaga tggaagagtg
     5581 ctcccaacat ttggaaaccg tgcggtttca atccggaggg tcagggagca agagtatgag
     5641 ttctatggtg tccgacacta gctgcacgtt cccctcaagt gatgggatat tctggaaaca
     5701 ctggatacag actaaagacg gacagtgtgg aagcccattg gtttccaccc gagatggttt
     5761 tatcgtgggt atccatagcg cctctaattt cacaaacacg aacaactatt tcacttcagt
     5821 gcccaaaaac tttatggagc tgctcacaaa ccaagaagcg cagcagtggg taagcggttg
     5881 gagacttaac gctgactcag ttctctgggg ggggcacaaa gtattcatgg taaagccaga
     5941 ggagccattc caaccagtca aagaagccac acaacttatg aacagctagc tcgaggaggg
     6001 cccgaacaaa aactcatctc agaagaggat ctgaatagcg ccgtcgacca tcatcatcat
     6061 catcattgag tttaaacccg ctgatcagcc tcgactgtgc cttctagttg ccagccatct
     6121 gttgtttgcc cctcccccgt gccttccttg accctggaag gtgccactcc cactgtcctt
     6181 tcctaataaa atgaggaaat tgcatcgcat tgtctgagta ggtgtcattc tattctgggg
     6241 ggtggggtgg ggcaggacag caagggggag gattgggaag acaatagcag gcatgctggg
     6301 ga
//
