LOCUS pSB1A3-AD009 4423 bp ds-DNA circular 10-DEC-2018 DEFINITION . FEATURES Location/Qualifiers terminator complement(482..538) /label="Terminator 310" /ApEinfo_revcolor=#b4abac /ApEinfo_fwdcolor=#b4abac Transcription activator complement(542..1465) /label="AraC" /ApEinfo_revcolor=#b1ff67 /ApEinfo_fwdcolor=#b1ff67 regulatory sequence 1604..1615 /label="araO1" /ApEinfo_revcolor=#ff9ccd /ApEinfo_fwdcolor=#ff9ccd regulatory sequence 1447..1462 /label="araO2" /ApEinfo_revcolor=#ff9ccd /ApEinfo_fwdcolor=#ff9ccd terminator 2717..2769 /label="Terminator 170n" /ApEinfo_revcolor=#b4abac /ApEinfo_fwdcolor=#b4abac cloning site 2798..2818 /label="BB Suffix" /ApEinfo_revcolor=#c7b0e3 /ApEinfo_fwdcolor=#c7b0e3 misc_feature complement(3756..4416) /label="AmpR (pLPT107)" /ApEinfo_revcolor=#f58a5e /ApEinfo_fwdcolor=#f58a5e regulatory sequence 1656..1694 /label="araI1I2" /ApEinfo_revcolor=#ff9ccd /ApEinfo_fwdcolor=#ff9ccd cloning site 432..453 /label="BB Prefix" /ApEinfo_revcolor=#c7b0e3 /ApEinfo_fwdcolor=#c7b0e3 ORI 2982..3694 /label="rep(pMB1)" /ApEinfo_revcolor=#f8d3a9 /ApEinfo_fwdcolor=#f8d3a9 inducible promoter 1691..1719 /label="araC promoter" /ApEinfo_revcolor=#f8d3a9 /ApEinfo_fwdcolor=#f8d3a9 misc_feature 1835..2710 /label="alpha-HL" /ApEinfo_revcolor=#f58a5e /ApEinfo_fwdcolor=#f58a5e RBS K=0.15 1795..1831 /label="B2" /ApEinfo_revcolor=#85dae9 /ApEinfo_fwdcolor=#85dae9 ORIGIN 1 aaacgttctt cggggcgaaa actctcaagg atcttaccgc tgttgagatc cagttcgata 61 taacccactc gtgcacccaa ctgatcttca gcatctttta ctttcaccag cgtttctggg 121 tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa taagggcgac acggaaatgt 181 tgaatactca tactcttcct ttttcaatat tattgaagca tttatcaggg ttattgtctc 241 atgagcggat acatatttga atgtatttag aaaaataaac aaataggggt tccgcgcaca 301 tttccccgaa aagtgccacc tgacgtctaa gaaaccatta ttatcatgac attaacctat 361 aaaaataggc gtatcacgag gcagaatttc agataaaaaa aatccttagc tttcgctaag 421 gatgatttct ggaattcgcg gccgcttcta gagGAGAGTT AGGCATAAGG CTGCATGCTA 481 CCCTTTGGTC GAAAAAAAAA GCCCGCACTG TCAGGTGCGG GCTTTTTTCT GTGTTTCCtt 541 atgacaactt gacggctaca tcattcactt tttcttcaca accggcacgg aactcgctcg 601 ggctggcccc ggtgcatttt ttaaataccc gcgagaaata gagttgatcg tcaaaaccaa 661 cattgcgacc gacggtggcg ataggcatcc gggtggtgct caaaagcagc ttcgcctggc 721 tgatacgttg gtcctcgcgc cagcttaaga cgctaatccc taactgctgg cggaaaagat 781 gtgacagacg cgacggcgac aagcaaacat gctgtgcgac gctggcgata tcaaaattgc 841 tgtctgccag gtgatcgctg atgtactgac aagcctcgcg tacccgatta tccatcggtg 901 gatggagcga ctcgttaatc gcttccatgc gccgcagtaa caattgctca agcagattta 961 tcgccagcag ctccgaatag cgcccttccc cttgcccggc gttaatgatt tgcccaaaca 1021 ggtcgctgaa atgcggctgg tgcgcttcat ccgggcgaaa gaaccccgta ttggcaaata 1081 ttgacggcca gttaagccat tcatgccagt aggcgcgcgg acgaaagtaa acccactggt 1141 gataccattc gcgagcctcc ggatgacgac cgtagtgatg aatctctcct ggcgggaaca 1201 gcaaaatatc acccggtcgg caaacaaatt ctcgtccctg atttttcacc accccctgac 1261 cgcgaatggt gagattgaga atataacctt tcattcccag cggtcggtcg ataaaaaaat 1321 cgagataacc gttggcctca atcggcgtta aacccgccac cagatgggca ttaaacgagt 1381 atcccggcag caggggatca ttttgcgctt cagccatact tttcatactc ccgccattca 1441 gagaagaaac caattgtcca tattgcatca gacattgccg tcactgcgtc ttttactggc 1501 tcttctcgct aaccaaaccg gtaaccccgc ttattaaaag cattctgtaa caaagcggga 1561 ccaaagccat gacaaaaacg cgtaacaaaa gtgtctataa tcacggcaga aaagtccaca 1621 ttgattattt gcacggcgtc acactttgct atgccatagc atttttatcc ataagattag 1681 cggatcctac ctgacgcttt ttatcgcaac tctctactgt ttctccatac ccgttttttt 1741 gggctagcga attcgagctc ggtacccggg gatcctctag aaggagatat aaccCTATGG 1801 ACTATGTTTT TCAAAGACGA AAAACTACTA GATGGATTCT GATATCAATA TCAAAACCGG 1861 CACCACCGAT ATCGGCTCCA ATACCACCGT TAAAACCGGT GATCTGGTGA CCTATGATAA 1921 AGAAAACGGT ATGCATAAAA AAGTGTTTTA CTCGTTTATT GACGATAAAA ACCATAACAA 1981 AAAACTGCTG GTCATCCGCA CCAAAGGCAC CATTGCGGGT CAATACCGTG TGTACTCCGA 2041 AGAAGGTGCG AACAAAAGCG GTCTGGCTTG GCCGTCTGCC TTTAAAGTGC AGCTGCAACT 2101 GCCGGATAAT GAAGTGGCGC AGATTTCAGA TTATTATCCG CGTAATAGCA TCGATACCAA 2161 AGAATATATG AGTACCCTGA CCTATGGTTT TAATGGCAAT GTTACCGGTG ATGATACGGG 2221 TAAAATTGGC GGTCTGATTG GCGCCAATGT GTCCATTGGT CATACGCTGA AATACGTGCA 2281 ACCGGATTTC AAAACCATTC TGGAAAGTCC GACCGATAAA AAAGTGGGTT GGAAAGTTAT 2341 CTTCAACAAC ATGGTGAATC AGAACTGGGG TCCGTACGAT CGCGATTCCT GGAATCCGGT 2401 TTATGGCAAT CAGCTGTTTA TGAAAACCCG CAACGGTAGT ATGAAAGCGG CGGATAATTT 2461 TCTGGACCCG AACAAAGCCT CAAGCCTGCT GTCCAGCGGT TTTAGCCCGG ATTTTGCCAC 2521 GGTTATTACC ATGGATCGCA AAGCCAGCAA ACAGCAGACC AACATTGATG TGATCTACGA 2581 ACGTGTGCGT GATGATTATC AACTGCATTG GACCTCAACC AATTGGAAAG GCACCAATAC 2641 CAAAGATAAA TGGACGGATC GCAGTTCAGA ACGCTACAAA ATTGATTGGG AAAAAGAAGA 2701 AATGACCAAC TAATAGAACG CATGAGAAAG CCCCCGGAAG ATCACCTTCC GGGGGCTTTT 2761 TTATTGCGCC TTGTCACACC TACACTGCTC GAAGtaCtac tagtagcggc cgctgcaggc 2821 ttcctcgctc actgactcgc tgcgctcggt cgttcggctg cggcgagcgg tatcagctca 2881 ctcaaaggcg gtaatacggt tatccacaga atcaggggat aacgcaggaa agaacatgtg 2941 agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca 3001 caggctccgc ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa 3061 cccgacagga ctataaagat accaggcgtt tccccctgga agctccctcg tgcgctctcc 3121 tgttccgacc ctgccgctta ccggatacct gtccgccttt ctcccttcgg gaagcgtggc 3181 gctttctcat agctcacgct gtaggtatct cagttcggtg taggtcgttc gctccaagct 3241 gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc gccttatccg gtaactatcg 3301 tcttgagtcc aacccggtaa gacacgactt atcgccactg gcagcagcca ctggtaacag 3361 gattagcaga gcgaggtatg taggcggtgc tacagagttc ttgaagtggt ggcctaacta 3421 cggctacact agaagaacag tatttggtat ctgcgctctg ctgaagccag ttaccttcgg 3481 aaaaagagtt ggtagctctt gatccggcaa acaaaccacc gctggtagcg gtggtttttt 3541 tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct caagaagatc ctttgatctt 3601 ttctacgggg tctgacgctc agtggaacga aaactcacgt taagggattt tggtcatgag 3661 attatcaaaa aggatcttca cctagatcct tttaaattaa aaatgaagtt ttaaatcaat 3721 ctaaagtata tatgagtaaa cttggtctga cagttaccaa tgcttaatca gtgaggcacc 3781 tatctcagcg atctgtctat ttcgttcatc catagttgcc tgactccccg tcgtgtagat 3841 aactacgata cgggagggct taccatctgg ccccagtgct gcaatgatac cgcgagaccc 3901 acgctcaccg gctccagatt tatcagcaat aaaccagcca gccggaaggg ccgagcgcag 3961 aagtggtcct gcaactttat ccgcctccat ccagtctatt aattgttgcc gggaagctag 4021 agtaagtagt tcgccagtta atagtttgcg caacgttgtt gccattgcta caggcatcgt 4081 ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc ggttcccaac gatcaaggcg 4141 agttacatga tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt 4201 tgtcagaagt aagttggccg cagtgttatc actcatggtt atggcagcac tgcataattc 4261 tcttactgtc atgccatccg taagatgctt ttctgtgact ggtgagtact caaccaagtc 4321 attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa tacgggataa 4381 taccgcgcca catagcagaa ctttaaaagt gctcatcatt gga //