LOCUS Exported 6291 bp ds-DNA circular SYN 20-FEB-2018 DEFINITION synthetic circular DNA ACCESSION . VERSION . KEYWORDS . SOURCE synthetic DNA construct ORGANISM recombinant plasmid REFERENCE 1 (bases 1 to 6291) AUTHORS Trial User TITLE Direct Submission JOURNAL Exported Aug 22, 2018 from SnapGene 4.2.4 http://www.snapgene.com FEATURES Location/Qualifiers source 1..6291 /organism="recombinant plasmid" /mol_type="other DNA" promoter 1..589 /label=CMV /note="CMV" misc_feature 1 /label=GFP tag /note="GFP tag" misc_feature 1 /label=Kanamycin /note="Kanamycin" promoter 508..589 /label=Pr_CMV /note="cytomegalovirus_minimal_promoter" misc_feature 601..645 /label=NES /note="Value" primer_bind complement(640..657) /label=BFPinsert-F misc_feature 658..1374 /label=EGFP /note="EGFP" primer_bind 658..675 /label=FPi-F /note="Amplify fluorescent protein from eGFP/CFP etc pr mCherry etc" primer_bind complement(661..678) /label=FPv-R /note="Amplify vector without eGFP/CFP etc pr mCherry etc. Use to exchange with FPi primers." primer_bind 1306..1326 /label=A41 primer_bind complement(1355..1374) /label=FPi-R /note="Amplify fluorescent protein from eGFP/CFP etc pr mCherry etc" primer_bind 1355..1374 /label=FPv-F /note="Amplify vector without eGFP/CFP etc pr mCherry etc. Use to exchange with FPi primers." misc_feature 1375..1404 /label=MCS /note="MCS" primer_bind complement(1390..1428) /label=RD-R primer_bind 1403..1428 /label=AUX1 TAPP1 F primer_bind 1412..1456 /label=RD-F CDS 1429..1911 /codon_start=1 /label=TAPP1-cPHx1 /translation="RNNLKRSQSHLPYFTPKPPQDSAVIKAGYCVKQGAVMKNWKRRYF QLDENTIGYFKSELEKEPLRVIPLKEVHKVQECKQSDIMMRDNLFEIVTTSRTFYVQAD SPEEMHSWIKAVSGAIVAQRGPGRSASSEHPPGPSESKHAFRPTNAATATSHSTASR" CDS 1429..1911 /codon_start=1 /label=TAPP1-cPHx2 /translation="RNNLKRSQSHLPYFTPKPPQDSAVIKAGYCVKQGAVMKNWKRRYF QLDENTIGYFKSELEKEPLRVIPLKEVHKVQECKQSDIMMRDNLFEIVTTSRTFYVQAD SPEEMHSWIKAVSGAIVAQRGPGRSASSEHPPGPSESKHAFRPTNAATATSHSTASR" primer_bind 1429..1456 /label=link-mid-F primer_bind complement(1884..1935) /label=link-R primer_bind complement(1901..1915) /label=AUX1 TAPP1 R primer_bind 1912..1963 /label=link-mid-F CDS 1936..2418 /codon_start=1 /label=TAPP1-cPHx1 /translation="RNNLKRSQSHLPYFTPKPPQDSAVIKAGYCVKQGAVMKNWKRRYF QLDENTIGYFKSELEKEPLRVIPLKEVHKVQECKQSDIMMRDNLFEIVTTSRTFYVQAD SPEEMHSWIKAVSGAIVAQRGPGRSASSEHPPGPSESKHAFRPTNAATATSHSTASR" CDS 1936..2418 /codon_start=1 /label=TAPP1-cPHx2 /translation="RNNLKRSQSHLPYFTPKPPQDSAVIKAGYCVKQGAVMKNWKRRYF QLDENTIGYFKSELEKEPLRVIPLKEVHKVQECKQSDIMMRDNLFEIVTTSRTFYVQAD SPEEMHSWIKAVSGAIVAQRGPGRSASSEHPPGPSESKHAFRPTNAATATSHSTASR" primer_bind 1936..1963 /label=RD-F primer_bind complement(2391..2442) /label=link-R primer_bind complement(2408..2422) /label=AUX1 TAPP1 R primer_bind 2419..2470 /label=link-mid-F CDS 2443..2925 /codon_start=1 /label=TAPP1-cPHx1 /translation="RNNLKRSQSHLPYFTPKPPQDSAVIKAGYCVKQGAVMKNWKRRYF QLDENTIGYFKSELEKEPLRVIPLKEVHKVQECKQSDIMMRDNLFEIVTTSRTFYVQAD SPEEMHSWIKAVSGAIVAQRGPGRSASSEHPPGPSESKHAFRPTNAATATSHSTASR" CDS 2443..2925 /codon_start=1 /label=TAPP1-cPHx2 /translation="RNNLKRSQSHLPYFTPKPPQDSAVIKAGYCVKQGAVMKNWKRRYF QLDENTIGYFKSELEKEPLRVIPLKEVHKVQECKQSDIMMRDNLFEIVTTSRTFYVQAD SPEEMHSWIKAVSGAIVAQRGPGRSASSEHPPGPSESKHAFRPTNAATATSHSTASR" primer_bind 2443..2470 /label=RD-F primer_bind complement(2898..2929) /label=link-R primer_bind complement(2915..2944) /label=AUX1 TAPP1 R misc_feature 2934..2977 /label=MCS /note="MCS" primer_bind complement(3027..3046) /label=Seq-PH-R primer_bind 3038..3060 /label=A42 misc_feature 3207..3662 /label=f1 /note="f1" promoter 3724..3752 /label=Bacterial /note="Bacterial" promoter 3836..4065 /label=SV40early /note="SV40early" misc_feature 4003..4138 /label=SV40 /note="SV40" misc_feature 4187..4981 /label=Kan/Neo /note="Kan/Neo" misc_feature 5217..5235 /label=HSV-TK /note="HSV-TK" misc_feature 5566..6209 /label=pUC /note="pUC" ORIGIN 1 tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata tggagttccg 61 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt 121 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca 181 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc 241 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta 301 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac 361 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg 421 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg 481 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt 541 acggtgggag gtctatataa gcagagctgg tttagtgaac cgtcagatcc gctagccacc 601 atggctctgc agaaaaagtt ggaagagctt gagctggatg aggcaccggt cgccaccatg 661 gtgagcaagg gcgaggagct gttcaccggg gtggtgccca tcctggtcga gctggacggc 721 gacgtaaacg gccacaagtt cagcgtgtcc ggcgagggcg agggcgatgc cacctacggc 781 aagctgaccc tgaagttcat ctgcaccacc ggcaagctgc ccgtgccctg gcccaccctc 841 gtgaccaccc tgacctacgg cgtgcagtgc ttcagccgct accccgacca catgaagcag 901 cacgacttct tcaagtccgc catgcccgaa ggctacgtcc aggagcgcac catcttcttc 961 aaggacgacg gcaactacaa gacccgcgcc gaggtgaagt tcgagggcga caccctggtg 1021 aaccgcatcg agctgaaggg catcgacttc aaggaggacg gcaacatcct ggggcacaag 1081 ctggagtaca actacaacag ccacaacgtc tatatcatgg ccgacaagca gaagaacggc 1141 atcaaggtga acttcaagat ccgccacaac atcgaggacg gcagcgtgca gctcgccgac 1201 cactaccagc agaacacccc catcggcgac ggccccgtgc tgctgcccga caaccactac 1261 ctgagcaccc agtccgccct gagcaaagac cccaacgaga agcgcgatca catggtcctg 1321 ctggagttcg tgaccgccgc cgggatcact ctcggcatgg acgagctgta caagtccgga 1381 ctcagatctc gagctcaagc ttcgaattct acctggaaaa tgagctcgag aaataatctg 1441 aaacggtcac aaagccatct tccttacttt actcctaaac cacctcaaga tagtgcggtt 1501 atcaaagctg gatattgtgt aaaacaagga gcagtgatga aaaactggaa gagaagatat 1561 tttcaattgg atgaaaacac aataggctac ttcaaatctg aactggaaaa ggaacctctt 1621 cgtgtaatac cacttaaaga ggttcataaa gtccaggaat gtaagcaaag cgacataatg 1681 atgagggaca acctctttga aattgtaaca acgtctcgaa ctttctatgt gcaggctgat 1741 agccctgaag agatgcacag ttggattaaa gcagtctctg gcgccattgt agcacagcgg 1801 ggtcccggca gatctgcgtc ttctgagcat ccccccggtc cttcagaatc caaacacgct 1861 ttccgtccta ccaacgcagc caccgccacc tcacattcca cagcctctcg cggaggctca 1921 gggggttccg gcggaagaaa taatctgaaa cggtcacaaa gccatcttcc ttactttact 1981 cctaaaccac ctcaagatag tgcggttatc aaagctggat attgtgtaaa acaaggagca 2041 gtgatgaaaa actggaagag aagatatttt caattggatg aaaacacaat aggctacttc 2101 aaatctgaac tggaaaagga acctcttcgt gtaataccac ttaaagaggt tcataaagtc 2161 caggaatgta agcaaagcga cataatgatg agggacaacc tctttgaaat tgtaacaacg 2221 tctcgaactt tctatgtgca ggctgatagc cctgaagaga tgcacagttg gattaaagca 2281 gtctctggcg ccattgtagc acagcggggt cccggcagat ctgcgtcttc tgagcatccc 2341 cccggtcctt cagaatccaa acacgctttc cgtcctacca acgcagccac cgccacctca 2401 cattccacag cctctcgcgg aggctcaggg ggttccggcg gaagaaataa tctgaaacgg 2461 tcacaaagcc atcttcctta ctttactcct aaaccacctc aagatagtgc ggttatcaaa 2521 gctggatatt gtgtaaaaca aggagcagtg atgaaaaact ggaagagaag atattttcaa 2581 ttggatgaaa acacaatagg ctacttcaaa tctgaactgg aaaaggaacc tcttcgtgta 2641 ataccactta aagaggttca taaagtccag gaatgtaagc aaagcgacat aatgatgagg 2701 gacaacctct ttgaaattgt aacaacgtct cgaactttct atgtgcaggc tgatagccct 2761 gaagagatgc acagttggat taaagcagtc tctggcgcca ttgtagcaca gcggggtccc 2821 ggcagatctg cgtcttctga gcatcccccc ggtccttcag aatccaaaca cgctttccgt 2881 cctaccaacg cagccaccgc cacctcacat tccacagcct ctcgctgagt cgacggtacc 2941 gcgggcccgg gatccaccgg atctagataa ctgatcataa tcagccatac cacatttgta 3001 gaggttttac ttgctttaaa aaacctccca cacctccccc tgaacctgaa acataaaatg 3061 aatgcaattg ttgttgttaa cttgtttatt gcagcttata atggttacaa ataaagcaat 3121 agcatcacaa atttcacaaa taaagcattt ttttcactgc attctagttg tggtttgtcc 3181 aaactcatca atgtatctta acgcgtaaat tgtaagcgtt aatattttgt taaaattcgc 3241 gttaaatttt tgttaaatca gctcattttt taaccaatag gccgaaatcg gcaaaatccc 3301 ttataaatca aaagaataga ccgagatagg gttgagtgtt gttccagttt ggaacaagag 3361 tccactatta aagaacgtgg actccaacgt caaagggcga aaaaccgtct atcagggcga 3421 tggcccacta cgtgaaccat caccctaatc aagttttttg gggtcgaggt gccgtaaagc 3481 actaaatcgg aaccctaaag ggagcccccg atttagagct tgacggggaa agccggcgaa 3541 cgtggcgaga aaggaaggga agaaagcgaa aggagcgggc gctagggcgc tggcaagtgt 3601 agcggtcacg ctgcgcgtaa ccaccacacc cgccgcgctt aatgcgccgc tacagggcgc 3661 gtcaggtggc acttttcggg gaaatgtgcg cggaacccct atttgtttat ttttctaaat 3721 acattcaaat atgtatccgc tcatgagaca ataaccctga taaatgcttc aataatattg 3781 aaaaaggaag agtcctgagg cggaaagaac cagctgtgga atgtgtgtca gttagggtgt 3841 ggaaagtccc caggctcccc agcaggcaga agtatgcaaa gcatgcatct caattagtca 3901 gcaaccaggt gtggaaagtc cccaggctcc ccagcaggca gaagtatgca aagcatgcat 3961 ctcaattagt cagcaaccat agtcccgccc ctaactccgc ccatcccgcc cctaactccg 4021 cccagttccg cccattctcc gccccatggc tgactaattt tttttattta tgcagaggcc 4081 gaggccgcct cggcctctga gctattccag aagtagtgag gaggcttttt tggaggccta 4141 ggcttttgca aagatcgatc aagagacagg atgaggatcg tttcgcatga ttgaacaaga 4201 tggattgcac gcaggttctc cggccgcttg ggtggagagg ctattcggct atgactgggc 4261 acaacagaca atcggctgct ctgatgccgc cgtgttccgg ctgtcagcgc aggggcgccc 4321 ggttcttttt gtcaagaccg acctgtccgg tgccctgaat gaactgcaag acgaggcagc 4381 gcggctatcg tggctggcca cgacgggcgt tccttgcgca gctgtgctcg acgttgtcac 4441 tgaagcggga agggactggc tgctattggg cgaagtgccg gggcaggatc tcctgtcatc 4501 tcaccttgct cctgccgaga aagtatccat catggctgat gcaatgcggc ggctgcatac 4561 gcttgatccg gctacctgcc cattcgacca ccaagcgaaa catcgcatcg agcgagcacg 4621 tactcggatg gaagccggtc ttgtcgatca ggatgatctg gacgaagagc atcaggggct 4681 cgcgccagcc gaactgttcg ccaggctcaa ggcgagcatg cccgacggcg aggatctcgt 4741 cgtgacccat ggcgatgcct gcttgccgaa tatcatggtg gaaaatggcc gcttttctgg 4801 attcatcgac tgtggccggc tgggtgtggc ggaccgctat caggacatag cgttggctac 4861 ccgtgatatt gctgaagagc ttggcggcga atgggctgac cgcttcctcg tgctttacgg 4921 tatcgccgct cccgattcgc agcgcatcgc cttctatcgc cttcttgacg agttcttctg 4981 agcgggactc tggggttcga aatgaccgac caagcgacgc ccaacctgcc atcacgagat 5041 ttcgattcca ccgccgcctt ctatgaaagg ttgggcttcg gaatcgtttt ccgggacgcc 5101 ggctggatga tcctccagcg cggggatctc atgctggagt tcttcgccca ccctaggggg 5161 aggctaactg aaacacggaa ggagacaata ccggaaggaa cccgcgctat gacggcaata 5221 aaaagacaga ataaaacgca cggtgttggg tcgtttgttc ataaacgcgg ggttcggtcc 5281 cagggctggc actctgtcga taccccaccg agaccccatt ggggccaata cgcccgcgtt 5341 tcttcctttt ccccacccca ccccccaagt tcgggtgaag gcccagggct cgcagccaac 5401 gtcggggcgg caggccctgc catagcctca ggttactcat atatacttta gattgattta 5461 aaacttcatt tttaatttaa aaggatctag gtgaagatcc tttttgataa tctcatgacc 5521 aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag accccgtaga aaagatcaaa 5581 ggatcttctt gagatccttt ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca 5641 ccgctaccag cggtggtttg tttgccggat caagagctac caactctttt tccgaaggta 5701 actggcttca gcagagcgca gataccaaat actgtccttc tagtgtagcc gtagttaggc 5761 caccacttca agaactctgt agcaccgcct acatacctcg ctctgctaat cctgttacca 5821 gtggctgctg ccagtggcga taagtcgtgt cttaccgggt tggactcaag acgatagtta 5881 ccggataagg cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag 5941 cgaacgacct acaccgaact gagataccta cagcgtgagc tatgagaaag cgccacgctt 6001 cccgaaggga gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc 6061 acgagggagc ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac 6121 ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac 6181 gccagcaacg cggccttttt acggttcctg gccttttgct ggccttttgc tcacatgttc 6241 tttcctgcgt tatcccctga ttctgtggat aaccgtatta ccgccatgca t //