LOCUS       Exported                9466 bp ds-DNA     circular SYN 23-AUG-2018
DEFINITION  synthetic circular DNA.
ACCESSION   .
VERSION     .
KEYWORDS    pMJA289=pSIn-EF2-Nanog-Pur+TRAV10+TRBV25+P2A
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9466)
  AUTHORS   Yu J, Vodyanik MA, Smuga-Otto K, Antosiewicz-Bourget J, Frane JL, 
            Tian S, Nie J, Jonsdottir GA, Ruotti V, Stewart R, Slukvin II, 
            Thomson JA
  TITLE     Induced Pluripotent Stem Cell Lines Derived from Human Somatic 
            Cells.
  JOURNAL   Science. 2007 Nov 20. ():.
REFERENCE   2  (bases 1 to 9466)
  AUTHORS   A
  TITLE     Direct Submission
  JOURNAL   Exported Aug 23, 2018 from SnapGene 4.2.4
            http://www.snapgene.com
COMMENT     ORIGDB|GenBank
FEATURES             Location/Qualifiers
     source          1..9466
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
                     /note="/vntifkey=98"
     LTR             117..750
                     /label=3' LTR
                     /label=3'\LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
                     /note="/vntifkey=19"
     misc_feature    797..922
                     /label=HIV-1 Psi
                     /label=HIV-1\Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
                     /note="/vntifkey=21"
     misc_feature    1415..1648
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
                     /note="/vntifkey=21"
     misc_feature    2164..2281
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
                     /note="/vntifkey=21"
     promoter        2409..3587
                     /label=EF-1-alpha promoter
                     /label=EF-1-alpha\promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
                     /note="/vntifkey=30"
     intron          2640..3578
                     /label=EF-1-alpha intron A
                     /label=EF-1-alpha\intron\A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
                     /note="/vntifkey=15"
     primer_bind     3535..3555
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
                     /note="/vntifkey=28"
     misc_feature    3610..4482
                     /label=TRAV10
                     /note="/vntifkey=21"
     misc_feature    4483..4548
                     /label=P2A cleavage sequence
                     /label=P2A\cleavage\sequence
                     /note="/vntifkey=21"
     misc_feature    4549..5496
                     /label=TRBV25
                     /note="/vntifkey=21"
     misc_feature    5582..6155
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
                     /note="/vntifkey=21"
     primer_bind     5749..5766
                     /label=IRES reverse
                     /label=IRES\reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
                     /note="/vntifkey=28"
     primer_bind     5976..5995
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
                     /note="/vntifkey=28"
     CDS             6188..6787
                     /codon_start=1
                     /gene="pac from Streptomyces"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /note="/vntifkey=4"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVECPKDRATWCMTRKPGA"
     primer_bind     6188..6207
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
                     /note="/vntifkey=28"
     primer_bind     6684..6704
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
                     /note="/vntifkey=28"
     LTR             6990..7223
                     /label=3' LTR (Delta-U3)
                     /label=3'\LTR\(Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
                     /note="/vntifkey=19"
     primer_bind     7374..7391
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
                     /note="/vntifkey=28"
     polyA_signal    7380..7604
                     /label=bGH poly(A) signal
                     /label=bGH\poly(A)\signal
                     /note="bovine growth hormone polyadenylation signal"
                     /note="/vntifkey=25"
     primer_bind     7656..7668
                     /label=M13 Reverse
                     /label=M13\Reverse
                     /note="In lacZ gene. Also called M13-rev"
                     /note="/vntifkey=28"
     promoter        7700..7804
                     /gene="bla"
                     /label=AmpR promoter
                     /label=AmpR\promoter
                     /note="/vntifkey=30"
     CDS             7805..8665
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /note="/vntifkey=4"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8023..8042
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
                     /note="/vntifkey=28"
     rep_origin      8836..9424
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
                     /note="/vntifkey=33"
     primer_bind     9325..9344
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
                     /note="/vntifkey=28"
ORIGIN
        1 cggaccgcca ctgccaatta cctgtggttt catttactct aaacctgtga ttcctctgaa
       61 ttattttcat tttaaagaaa ttgtatttgt taaatatgta ctacaaactt agtagttgga
      121 agggctaatt cactcccaaa gaagacaaga tatccttgat ctgtggatct accacacaca
      181 aggctacttc cctgattagc agaactacac accagggcca ggggtcagat atccactgac
      241 ctttggatgg tgctacaagc tagtaccagt tgagccagat aaggtagaag aggccaataa
      301 aggagagaac accagcttgt tacaccctgt gagcctgcat gggatggatg acccggagag
      361 agaagtgtta gagtggaggt ttgacagccg cctagcattt catcacgtgg cccgagagct
      421 gcatccggag tacttcaaga actgctgata tcgagcttgc tacaagggac tttccgctgg
      481 ggactttcca gggaggcgtg gcctgggcgg gactggggag tggcgagccc tcagatcctg
      541 catataagca gctgcttttt gcctgtactg ggtctctctg gttagaccag atctgagcct
      601 gggagctctc tggctaacta gggaacccac tgcttaagcc tcaataaagc ttgccttgag
      661 tgcttcaagt agtgtgtgcc cgtctgttgt gtgactctgg taactagaga tccctcagac
      721 ccttttagtc agtgtggaaa atctctagca gtggcgcccg aacagggact tgaaagcgaa
      781 agggaaacca gaggagctct ctcgacgcag gactcggctt gctgaagcgc gcacggcaag
      841 aggcgagggg cggcgactgg tgagtacgcc aaaaattttg actagcggag gctagaagga
      901 gagagatggg tgcgagagcg tcagtattaa gcgggggaga attagatcgc gatgggaaaa
      961 aattcggtta aggccagggg gaaagaaaaa atataaatta aaacatatag tatgggcaag
     1021 cagggagcta gaacgattcg cagttaatcc tggcctgtta gaaacatcag aaggctgtag
     1081 acaaatactg ggacagctac aaccatccct tcagacagga tcagaagaac ttagatcatt
     1141 atataataca gtagcaaccc tctattgtgt gcatcaaagg atagagataa aagacaccaa
     1201 ggaagcttta gacaagatag aggaagagca aaacaaaagt aagaccaccg cacagcaagc
     1261 ggccgctgat cttcagacct ggaggaggag atatgaggga caattggaga agtgaattat
     1321 ataaatataa agtagtaaaa attgaaccat taggagtagc acccaccaag gcaaagagaa
     1381 gagtggtgca gagagaaaaa agagcagtgg gaataggagc tttgttcctt gggttcttgg
     1441 gagcagcagg aagcactatg ggcgcagcgt caatgacgct gacggtacag gccagacaat
     1501 tattgtctgg tatagtgcag cagcagaaca atttgctgag ggctattgag gcgcaacagc
     1561 atctgttgca actcacagtc tggggcatca agcagctcca ggcaagaatc ctggctgtgg
     1621 aaagatacct aaaggatcaa cagctcctgg ggatttgggg ttgctctgga aaactcattt
     1681 gcaccactgc tgtgccttgg aatgctagtt ggagtaataa atctctggaa cagatttgga
     1741 atcacacgac ctggatggag tgggacagag aaattaacaa ttacacaagc ttaatacact
     1801 ccttaattga agaatcgcaa aaccagcaag aaaagaatga acaagaatta ttggaattag
     1861 ataaatgggc aagtttgtgg aattggttta acataacaaa ttggctgtgg tatataaaat
     1921 tattcataat gatagtagga ggcttggtag gtttaagaat agtttttgct gtactttcta
     1981 tagtgaatag agttaggcag ggatattcac cattatcgtt tcagacccac ctcccaaccc
     2041 cgaggggacc cgacaggccc gaaggaatag aagaagaagg tggagagaga gacagagaca
     2101 gatccattcg attagtgaac ggatctcgac ggtatcgcca caaatggcag tattcatcca
     2161 caattttaaa agaaaggggg ggattggggg gtacagtgca ggggaaagaa tagtagacat
     2221 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaaa ttcaaaattt
     2281 tcgggtttat tacagggaca gcagagatcc actttggtcg ataagctttg caaagatgga
     2341 taaagtttta aacagagagg aatctttgca gctaatggac cttctaggtc ttgaaaggag
     2401 tgggaattgg ctccggtgcc cgtcagtggg cagagcgcac atcgcccaca gtccccgaga
     2461 agttgggggg aggggtcggc aattgaaccg gtgcctagag aaggtggcgc ggggtaaact
     2521 gggaaagtga tgtcgtgtac tggctccgcc tttttcccga gggtggggga gaaccgtata
     2581 taagtgcagt agtcgccgtg aacgttcttt ttcgcaacgg gtttgccgcc agaacacagg
     2641 taagtgccgt gtgtggttcc cgcgggcctg gcctctttac gggttatggc ccttgcgtgc
     2701 cttgaattac ttccacctgg ctgcagtacg tgattcttga tcccgagctt cgggttggaa
     2761 gtgggtggga gagttcgagg ccttgcgctt aaggagcccc ttcgcctcgt gcttgagttg
     2821 aggcctggcc tgggcgctgg ggccgccgcg tgcgaatctg gtggcacctt cgcgcctgtc
     2881 tcgctgcttt cgataagtct ctagccattt aaaatttttg atgacctgct gcgacgcttt
     2941 ttttctggca agatagtctt gtaaatgcgg gccaagatct gcacactggt atttcggttt
     3001 ttggggccgc gggcggcgac ggggcccgtg cgtcccagcg cacatgttcg gcgaggcggg
     3061 gcctgcgagc gcggccaccg agaatcggac gggggtagtc tcaagctggc cggcctgctc
     3121 tggtgcctgg cctcgcgccg ccgtgtatcg ccccgccctg ggcggcaagg ctggcccggt
     3181 cggcaccagt tgcgtgagcg gaaagatggc cgcttcccgg ccctgctgca gggagctcaa
     3241 aatggaggac gcggcgctcg ggagagcggg cgggtgagtc acccacacaa aggaaaaggg
     3301 cctttccgtc ctcagccgtc gcttcatgtg actccacgga gtaccgggcg ccgtccaggc
     3361 acctcgatta gttctcgagc ttttggagta cgtcgtcttt aggttggggg gaggggtttt
     3421 atgcgatgga gtttccccac actgagtggg tggagactga agttaggcca gcttggcact
     3481 tgatgtaatt ctccttggaa tttgcccttt ttgagtttgg atcttggttc attctcaagc
     3541 ctcagacagt ggttcaaagt ttttttcttc catttcaggt gtcgtgagga attgatcctt
     3601 cgaactagta tgaaacaagg cagcagattt gaggcaggct tctccagaac aaccatgaag
     3661 aagcacctga ccacctttct cgtgatcctg tggctgtact tctacagagg caacggcaag
     3721 aaccaggtgg aacagagccc ccagagcctg atcatcctgg aaggcaagaa ctgcaccctg
     3781 cagtgcaact acaccgtgtc cccattcagc aacctgagat ggtacaagca ggacaccggc
     3841 agaggccccg tgtccctgac catcatgacc ttcagcgaga acaccaagag caacggcaga
     3901 tacaccgcca ccctggacgc cgacacaaag cagagcagcc tgcacatcac cgccagccag
     3961 ctgagcgaca gcgccagcta catctgcgtg gtgtccgaca gaggcagcac cctgggcaga
     4021 ctgtacttcg gcagaggaac ccagctgacc gtgtggcccg acatccagaa ccctgaccct
     4081 gccgtgtacc agctgagaga ctccaagtcc agcgacaaga gcgtgtgcct gttcaccgac
     4141 ttcgacagcc agaccaacgt gtcccagagc aaggacagcg acgtgtacat caccgacaag
     4201 accgtgctgg acatgagatc catggacttc aagtctaaca gcgccgtggc ctggtccaac
     4261 aagagcgact tcgcctgcgc caacgccttc aacaacagca ttatccccga ggacacattc
     4321 ttcccaagcc ccgagagcag ctgcgacgtg aagctggtgg aaaagagctt cgagacagac
     4381 accaacctga acttccagaa cctgagcgtg atcggcttca gaatcctgct gctgaaggtg
     4441 gccggcttca acctgctgat gaccctgaga ctgtggtcca gcggaagcgg agctactaac
     4501 ttcagcctgc tgaagcaggc tggagacgtg gaggacaacc ctggacctat gaccatcaga
     4561 ctgctgtgct acgtgggctt ctacttcctg ggcgctggcc tgatggaagc cgacatctac
     4621 cagaccccca gatacctcgt gatcggcacc ggcaagaaaa tcaccctgga atgcagccag
     4681 accatgggcc acgacaagat gtactggtat cagcaggacc ccggcatgga actgcacctg
     4741 atccactaca gctacggcgt gaacagcacc gagaagggcg acctgagcag cgagagcacc
     4801 gtgtccagaa tcagaaccga gcacttccca ctgaccctgg aaagcgctag acccagccac
     4861 accagccagt acctgtgtgc cagcagctct cctctgggcg gaacacctgg cgccaacgtg
     4921 ctgacatttg gcgctggcag cagactgacc gtgctggaag atctgaagaa cgtgttccca
     4981 cccgaggtgg ccgtgttcga gccttctgag gccgagatct cccacaccca gaaagccacc
     5041 ctcgtgtgtc tggccaccgg cttctacccc gaccacgtgg aactgtcttg gtgggtcaac
     5101 ggcaaagagg tgcacagcgg cgtgtccacc gatccccagc ctctgaaaga gcagcccgcc
     5161 ctgaacgaca gcagatactg cctgtccagc aggctgagag tgtccgccac cttctggcag
     5221 aaccccagaa accacttcag atgccaggtg cagttttacg gcctgagcga gaacgacgag
     5281 tggacccagg acagagccaa gcccgtgacc cagatcgtgt ctgctgaggc ctggggcaga
     5341 gccgactgtg gcttcacaag cgagagctac cagcagggcg tgctgagcgc caccatcctg
     5401 tacgagatcc tgctgggcaa ggccaccctg tacgccgtgc tggtgtctgc cctggtgctg
     5461 atggccatgg tcaagagaaa ggacagcaga ggctgagctt gaattcgaag cttgagctcg
     5521 agatctgagt ccggtagcgc tagttcgaag gatccgcatg catctagggc ggccaattcc
     5581 gcccctctcc ctcccccccc cctaacgtta ctggccgaag ccgcttggaa taaggccggt
     5641 gtgcgtttgt ctatatgtga ttttccacca tattgccgtc ttttggcaat gtgagggccc
     5701 ggaaacctgg ccctgtcttc ttgacgagca ttcctagggg tctttcccct ctcgccaaag
     5761 gaatgcaagg tctgttgaat gtcgtgaagg aagcagttcc tctggaagct tcttgaagac
     5821 aaacaacgtc tgtagcgacc ctttgcaggc agcggaaccc cccacctggc gacaggtgcc
     5881 tctgcggcca aaagccacgt gtataagata cacctgcaaa ggcggcacaa ccccagtgcc
     5941 acgttgtgag ttggatagtt gtggaaagag tcaaatggct ctcctcaagc gtattcaaca
     6001 aggggctgaa ggatgcccag aaggtacccc attgtatggg atctgatctg gggcctcggt
     6061 gcacatgctt tacatgtgtt tagtcgaggt taaaaaaacg tctaggcccc ccgaaccacg
     6121 gggacgtggt tttcctttga aaaacacgat gataagcttg ccacaaccca caaggagacg
     6181 accttccatg accgagtaca agcccacggt gcgcctcgcc acccgcgacg acgtcccccg
     6241 ggccgtacgc accctcgccg ccgcgttcgc cgactacccc gccacgcgcc acaccgtcga
     6301 cccggaccgc cacatcgagc gggtcaccga gctgcaagaa ctcttcctca cgcgcgtcgg
     6361 gctcgacatc ggcaaggtgt gggtcgcgga cgacggcgcc gcggtggcgg tctggaccac
     6421 gccggagagc gtcgaagcgg gggcggtgtt cgccgagatc ggcccgcgca tggccgagtt
     6481 gagcggttcc cggctggccg cgcagcaaca gatggaaggc ctcctggcgc cgcaccggcc
     6541 caaggagccc gcgtggttcc tggccaccgt cggcgtctcg cccgaccacc agggcaaggg
     6601 tctgggcagc gccgtcgtgc tccccggagt ggaggcggcc gagcgcgccg gggtgcccgc
     6661 cttcctggag acctccgcgc cccgcaacct ccccttctac gagcggctcg gcttcaccgt
     6721 caccgccgac gtcgagtgcc cgaaggaccg cgcgacctgg tgcatgaccc gcaagcccgg
     6781 tgcctgataa taggcggccg ctcgagacct agaaaaacat ggagcaatca caagtagcaa
     6841 tacagcagct accaatgctg attgtgcctg gctagaagca caagaggagg aggaggtggg
     6901 ttttccagtc acacctcagg tacctttaag accaatgact tacaaggcag ctgtagatct
     6961 tagccacttt ttaaaagaaa aggggggact ggaagggcta attcactccc aacgaagaca
     7021 agatctgctt tttgcttgta ctgggtctct ctggttagac cagatctgag cctgggagct
     7081 ctctggctaa ctagggaacc cactgcttaa gcctcaataa agcttgcctt gagtgcttca
     7141 agtagtgtgt gcccgtctgt tgtgtgactc tggtaactag agatccctca gaccctttta
     7201 gtcagtgtgg aaaatctcta gcagtagtag ttcatgtcat cttattattc agtatttata
     7261 acttgcaaag aaatgaatat cagagagtga gaggccttga cattataata gatttagcag
     7321 gaattgaact aggagtggag cacacaggca aagttctaga gctcgctgat cagcctcgac
     7381 tgtgccttct agttgccagc catctgttgt ttgcccctcc cccgtgcctt ccttgaccct
     7441 ggaaggtgcc actcccactg tcctttccta ataaaatgag gaaattgcat cgcattgtct
     7501 gagtaggtgt cattctattc tggggggtgg ggtggggcag gacagcaagg gggaggattg
     7561 ggaagacaat agcaggcatg ctggggatgc ggtgggctct atggcttctg aggcggaaag
     7621 aaccagctgg gggcgcgccc ctcgaggccg ccatggtcat agctgtttga cgtcaggtgg
     7681 cacttttcgg ggaaatgtgc gcggaacccc tatttgttta tttttctaaa tacattcaaa
     7741 tatgtatccg ctcatgagac aataaccctg ataaatgctt caataatatt gaaaaaggaa
     7801 gagtatgagt attcaacatt tccgtgtcgc ccttattccc ttttttgcgg cattttgcct
     7861 tcctgttttt gctcacccag aaacgctggt gaaagtaaaa gatgctgaag atcagttggg
     7921 tgcacgagtg ggttacatcg aactggatct caacagcggt aagatccttg agagttttcg
     7981 ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt
     8041 atcccgtatt gacgccgggc aagagcaact cggtcgccgc atacactatt ctcagaatga
     8101 cttggttgag tactcaccag tcacagaaaa gcatcttacg gatggcatga cagtaagaga
     8161 attatgcagt gctgccataa ccatgagtga taacactgcg gccaacttac ttctgacaac
     8221 gatcggagga ccgaaggagc taaccgcttt tttgcacaac atgggggatc atgtaactcg
     8281 ccttgatcgt tgggaaccgg agctgaatga agccatacca aacgacgagc gtgacaccac
     8341 gatgcctgta gcaatggcaa caacgttgcg caaactatta actggcgaac tacttactct
     8401 agcttcccgg caacaattaa tagactggat ggaggcggat aaagttgcag gaccacttct
     8461 gcgctcggcc cttccggctg gctggtttat tgctgataaa tctggagccg gtgagcgtgg
     8521 gtctcgcggt atcattgcag cactggggcc agatggtaag ccctcccgta tcgtagttat
     8581 ctacacgacg gggagtcagg caactatgga tgaacgaaat agacagatcg ctgagatagg
     8641 tgcctcactg attaagcatt ggtaactgtc agaccaagtt tactcatata tactttagat
     8701 tgatttaaaa cttcattttt aatttaaaag gatctaggtg aagatccttt ttgataatct
     8761 catgaccaaa atcccttaac gtgagttttc gttccactga gcgtcagacc ccgtagaaaa
     8821 gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa
     8881 aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc
     8941 gaaggtaact ggcttcagca gagcgcagat accaaatact gtccttctag tgtagccgta
     9001 gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct
     9061 gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg
     9121 atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag
     9181 cttggagcga acgacctaca ccgaactgag atacctacag cgtgagcatt gagaaagcgc
     9241 cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg
     9301 agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt
     9361 tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg
     9421 gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc ttttgc
//
