LOCUS       Exported               10699 bp ds-DNA     circular SYN 23-AUG-2018
DEFINITION  Control of the E3 ligase Bre1 with LINXa in yeast..
ACCESSION   .
VERSION     .
KEYWORDS    pNIA-CEN-FLAG-LINXa4(RVH)-Bre1
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10699)
  AUTHORS   Kuhlman
  TITLE     Kuhlman Lab LINX plasmids
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 10699)
  AUTHORS   Hayretin Yumerefendi
  TITLE     Direct Submission
  JOURNAL   Exported Aug 23, 2018 from SnapGene Viewer 3.0.3
            http://www.snapgene.com
COMMENT     ORIGDB|GenBank
FEATURES             Location/Qualifiers
     source          1..10699
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     promoter        88..118
                     /note="lac promoter"
                     /note="promoter for the E. coli lac operon"
     protein_bind    126..142
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="lac operator"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     131..153
                     /note="M13/pUC Reverse"
                     /note="In lacZ gene"
     primer_bind     150..166
                     /note="M13 rev"
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     150..166
                     /note="M13 Reverse"
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     185..205
                     /note="T3"
                     /note="T3 promoter, forward primer"
     promoter        187..205
                     /note="T3 promoter"
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        1310..1710
                     /gene="S. cerevisiae ADH1"
                     /note="ADH1 promoter"
                     /note="promoter for alcohol dehydrogenase 1"
     misc_feature    1737..2285
                     /note="LINXa4"
     CDS             1764..1787
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /note="FLAG"
                     /translation="DYKDDDDK"
     CDS             2310..4409
                     /codon_start=1
                     /note="Bre1"
                     /translation="GTAEPATAAIALELSDPSEPLTQSDVIAFQKEALFRCINRRRVDF
                     EALRKQYELSRRECIDVSRKLANIMALIVTLARFIETFCTDANEKQLCREIAQGDETLI
                     VQRSDSFMKLLTKYGKPNTTDSNTNSNASDHIQELTTELKNLRKSKEELFYENSQLTEE
                     ISALKEYYTNIIRKYDRDESFTIKRVFKEDKTDAVKELREDEKESNENNIKSGNKDSSA
                     INGDNTSKKSEKGDELVQAEDERKEDAENEKLELDLKFSDLRAEINSLSSTIKDLENIR
                     RENEEELIKTRSEVSNLKKQQIAAADQDPDFKSYDHESLLAKIQHLTEQNAELSEINSS
                     FLSKFQVLAKEKEIYTKKVREEFQKSLDSLVEMNSSLEKDVVRIRTARDDLLSKIAILE
                     AEKSKTEVLSDLQHAIDILKKQWTKIDQRSNDTKSSSTQDALIKEIQDLEKGFRELSDL
                     THKKYSEIINHESVISKLTVEKTKADQKYFAAMRSKDSILIEIKTLSKSLSKSNELILQ
                     LKDSDRLLQQKIGNLHKQLDLSQNNERRLIDSSKTETLKIIDLNNTSTKLKRSLEKLQE
                     ESNKSIADMTHLETKLNDTEIELKHFKQKASHLESKCEKLHDTLFRGNNKNKGSSDEAL
                     VEELANFRTLVYCSLCSKNWKNMAIKTCGHVFCENCCKERLAARMRKCPTCNKAFSSND
                     LLTVHL"
     terminator      4431..4678
                     /gene="S. cerevisiae CYC1"
                     /note="CYC1 terminator"
                     /note="transcription terminator for CYC1"
     primer_bind     complement(4447..4465)
                     /note="CYC1"
                     /note="CYC1 transcription termination signal, reverse 
                     primer"
     primer_bind     complement(4696..4715)
                     /note="T7"
                     /note="T7 promoter, forward primer"
     promoter        complement(4697..4715)
                     /note="T7 promoter"
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(4725..4742)
                     /note="M13 Forward"
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(4725..4741)
                     /note="M13 fwd"
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4734..4756)
                     /note="M13/pUC Forward"
                     /note="In lacZ gene"
     primer_bind     complement(4877..4894)
                     /note="L4440"
                     /note="L4440 vector, forward primer"
     rep_origin      complement(5048..5636)
                     /direction=LEFT
                     /note="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(5128..5147)
                     /note="pBR322ori-F"
                     /note="pBR322 origin, forward primer"
     CDS             complement(5807..6667)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /note="AmpR"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     6430..6449
                     /note="Amp-R"
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(6668..6772)
                     /gene="bla"
                     /note="AmpR promoter"
     primer_bind     6840..6858
                     /note="pBRforEco"
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        6878..7282
                     /gene="S. cerevisiae LEU2"
                     /note="LEU2 promoter"
     CDS             7295..8389
                     /codon_start=1
                     /gene="S. cerevisiae LEU2"
                     /product="3-isopropylmalate dehydrogenase, required for 
                     leucine biosynthesis"
                     /note="LEU2"
                     /note="yeast auxotrophic marker"
                     /translation="MSAPKKIVVLPGDHVGQEITAEAIKVLKAISDVRSNVKFDFENHL
                     IGGAAIDATGVPLPDEALEASKKVDAVLLGAVGGPKWGTGSVRPEQGLLKIRKELQLYA
                     NLRPCNFASDSLLDLSPIKPQFAKGTDFVVVRELVGGIYFGKRKEDDGDGVAWDSEQYT
                     VPEVQRITRMAAFMALQHEPPLPIWSLDKANVLASSRLWRKTVEETIKNEFPTLKVQHQ
                     LIDSAAMILVKNPTHLNGIIITSNMFGDIISDEASVIPGSLGLLPSASLASLPDKNTAF
                     GLYEPCHGSAPDLPKNKVDPIATILSAAMMLKLSLNLPEEGKAIEDAVKKVLDAGIRTG
                     DLGGSNSTTEVGDAVAEEVKKILA"
     misc_feature    8751..9913
                     /note="CEN/ARS"
                     /note="S. cerevisiae CEN4 centromere fused to the 
                     autonomously replicating sequence ARS1/ARS416"
     rep_origin      8751..9588
                     /note="ARS1"
                     /note="S. cerevisiae autonomously replicating sequence 
                     ARS1/ARS416"
ORIGIN
        1 ctggcacgac aggtttcccg actggaaagc gggcagtgag cgcaacgcaa ttaatgtgag
       61 ttacctcact cattaggcac cccaggcttt acactttatg cttccggctc ctatgttgtg
      121 tggaattgtg agcggataac aatttcacac aggaaacagc tatgaccatg attacgccaa
      181 gcgcgcaatt aaccctcact aaagggaaca aaagctggag ctctaaaaca agaagagggt
      241 tgactacatc acgatgaggg ggatcgaaga aatgatggta aatgaaatag gaaatcaagg
      301 agcatgaagg caaaagacaa atataagggt cgaacgaaaa ataaagtgaa aagtgttgat
      361 atgatgtatt tggctttgcg gcgccgaaaa aacgagttta cgcaattgca caatcatgct
      421 gactctgtgg cggacccgcg ctcttgccgg cccggcgata acgctgggcg tgaggctgtg
      481 cccggcggag ttttttgcgc ctgcattttc caaggtttac cctgcgctaa ggggcgagat
      541 tggagaagca ataagaatgc cggttggggt tgcgatgatg acgaccacga caactggtgt
      601 cattatttaa gttgccgaaa gaacctgagt gcatttgcaa catgagtata ctagaagaat
      661 gagccaagac ttgcgagacg cgagtttgcc ggtggtgcga acaatagagc gaccatgacc
      721 ttgaaggtga gacgcgcata accgctagag tactttgaag aggaaacagc aatagggttg
      781 ctaccagtat aaatagacag gtacatacaa cactggaaat ggttgtctgt ttgagtacgc
      841 tttcaattca tttgggtgtg cactttatta tgttacaata tggaagggaa ctttacactt
      901 ctcctatgca catatattaa ttaaagtcca atgctagtag agaagggggg taacacccct
      961 ccgcgctctt ttccgatttt tttctaaacc gtggaatatt tcggatatcc ttttgttgtt
     1021 tccgggtgta caatatggac ttcctctttt ctggcaacca aacccataca tcgggattcc
     1081 tataatacct tcgttggtct ccctaacatg taggtggcgg aggggagata tacaatagaa
     1141 cagataccag acaagacata atgggctaaa caagactaca ccaattacac tgcctcattg
     1201 atggtggtac ataacgaact aatactgtag ccctagactt gatagccatc atcatatcga
     1261 agtttcacta ccctttttcc atttgccatc tattgaagta ataataggcg catgcaactt
     1321 cttttctttt tttttctttt ctctctcccc cgttgttgtc tcaccatatc cgcaatgaca
     1381 aaaaaaatga tggaagacac taaaggaaaa aattaacgac aaagacagca ccaacagatg
     1441 tcgttgttcc agagctgatg aggggtatct tcgaacacac gaaacttttt ccttccttca
     1501 ttcacgcaca ctactctcta atgagcaacg gtatacggcc ttccttccag ttacttgaat
     1561 ttgaaataaa aaaagtttgc tgtcttgcta tcagtataaa tagacctgca attattaatc
     1621 ttttgtttcc tcgtcattgt tctcgttccc tttcttcctt gtttcttttt ctgcacaata
     1681 tttcaagcta taccaagcat acaatcaact ccaagcttga attaattccg ggcggaatga
     1741 aagcgttaac gtctggcggc cgcgattaca aggacgacga tgacaagtct agagccgccg
     1801 ccaagagggt gaagctggac ggttctggaa gtgggtcctt ggctactaca cttgaacgta
     1861 ttgagaagaa ctttgtcatt actgacccaa gattgccaga taatcccatt atattcgcgt
     1921 ccgatagttt cttgcagttg acagaatata gccgtgaaga aattttggga agaaactgca
     1981 ggtttctaca aggtcctgaa actgatcgcg cgacagtgag aaaaattaga gatgccatag
     2041 ataaccaaac agaggtcact gttcagctga ttaattatac aaagagtggt aaaaagttct
     2101 ggaacctctt tcacttgcag cctatgcgag atcagaaggg agatgtccag tactttattg
     2161 gggttcagtt ggatggaact gagcgagtcc acgatgctgc cgagagagag ggagtcatgc
     2221 tgattaagaa aactgcagaa aatattgatg aagcggcaaa agagctggcc gggctggatc
     2281 tggggtctgg aagtgggtcg gggtcccggg gaacggccga gcctgctact gccgctatcg
     2341 cactagaatt aagcgatcct agtgaaccgc ttactcaatc tgatgtgata gcgtttcaaa
     2401 aagaagcttt gtttagatgt ataaatcgtc gaagagtcga ctttgaagcc ttgcgaaagc
     2461 aatatgaact ttcaagaagg gagtgcatag atgtttcccg caagttggca aatattatgg
     2521 cgctgatagt aactttagct aggtttatcg aaacattctg cacagacgcc aacgagaagc
     2581 aactatgtag ggaaatagcc caaggcgatg agacgttgat agttcagcgc agtgattctt
     2641 tcatgaagct actgactaaa tatgggaagc caaacacgac agatagtaat actaatagta
     2701 atgcttcgga tcatattcaa gaattaacaa cggagttaaa aaacttgcgg aaaagtaaag
     2761 aggagctctt ttatgaaaat tcgcaattga cagaggagat ttctgctttg aaggaatatt
     2821 atacaaatat aattcgcaag tatgatagag atgaatcctt tactataaaa cgtgttttta
     2881 aggaagataa gactgatgct gtaaaggaat tacgggaaga tgaaaaagaa tctaatgaaa
     2941 acaatataaa atctggaaat aaagactcct ctgcaattaa tggtgataat acatccaaaa
     3001 aatccgagaa aggtgatgaa cttgtgcagg cggaggatga acgaaaagaa gatgctgaga
     3061 atgaaaaatt agaattggat ctgaaatttt ctgatttacg ggcagagata aactccttga
     3121 gttccactat aaaagacctt gaaaatatca gaagggaaaa cgaggaagag ctcatcaaaa
     3181 cacgttctga ggtatccaat ttaaaaaagc aacagatagc agcagcagac caagacccag
     3241 atttcaagtc atatgaccat gagtccctgt tagcaaaaat tcaacatttg actgaacaaa
     3301 acgcagaatt atctgagatc aatagttcct ttttatcaaa atttcaagtt ttagccaaag
     3361 agaaagaaat atatacgaag aaggtccgag aagaattcca gaaaagtctc gactccctag
     3421 tagaaatgaa cagttcattg gaaaaggacg ttgtaagaat aagaacggct cgtgatgatt
     3481 tactaagcaa aatcgccatt ttggaagctg aaaaatccaa gactgaagtg ttgagcgatt
     3541 tacaacatgc aattgatatt ttgaagaagc aatggacaaa aattgatcaa agatcaaacg
     3601 atacaaaatc ctcgtcgact caagatgcat taataaagga aattcaagac ttagagaagg
     3661 gttttagaga gttatccgac ttaactcata aaaagtactc tgagattatt aatcacgaat
     3721 ctgttatatc caagcttacc gtagaaaaaa ctaaggctga tcagaaatat tttgccgcga
     3781 tgaggtccaa agattcaatt ttaattgaaa tcaaaactct ttctaaaagc ctcagtaaat
     3841 caaatgagct tattctacag ttaaaagact ctgatagatt gctacagcaa aaaataggaa
     3901 atttacataa acaactggat ctttctcaaa ataatgaacg caggttaatt gattccagta
     3961 aaacagagac actaaaaata attgatttaa ataatacatc cactaaactg aaaaggtcat
     4021 tagaaaagct acaagaagaa agtaacaaaa gcattgcaga tatgacgcat ctagaaacta
     4081 aattaaatga tacagaaata gaattgaagc acttcaagca gaaggcatct catctagaat
     4141 ccaagtgtga aaaactacat gatacattgt tccgtggtaa caacaaaaat aagggctcga
     4201 gcgatgaagc actcgtagaa gaactagcaa atttccgtac acttgtttat tgttccctgt
     4261 gttctaaaaa ttggaaaaac atggctatta aaacctgtgg ccatgtcttt tgtgagaatt
     4321 gctgtaagga acgattggca gcgagaatga gaaaatgccc tacatgcaac aaagcattca
     4381 gttccaatga tttattgaca gtgcacttgt aaggatccgt cgacctcgag tcatgtaatt
     4441 agttatgtca cgcttacatt cacgccctcc ccccacatcc gctctaaccg aaaaggaagg
     4501 agttagacaa cctgaagtct aggtccctat ttattttttt atagttatgt tagtattaag
     4561 aacgttattt atatttcaaa tttttctttt ttttctgtac aaacgcgtgt acgcatgtaa
     4621 cattatactg aaaaccttgc ttgagaaggt tttgggacgc tcgaaggctt taatttgcgg
     4681 ccggtaccca attcgcccta tagtgagtcg tattacgcgc gctcactggc cgtcgtttta
     4741 caacgtcgtg actgggaaaa ccctggcgtt acccaactta atcgccttgc agcacatccc
     4801 cctttcgcca gctgcattaa tgaatcggcc aacgcgcggg gagaggcggt ttgcgtattg
     4861 ggcgctcttc cgcttcctcg ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag
     4921 cggtatcagc tcactcaaag gcggtaatac ggttatccac agaatcaggg gataacgcag
     4981 gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc
     5041 tggcgttttt ccataggctc cgcccccctg acgagcatca caaaaatcga cgctcaagtc
     5101 agaggtggcg aaacccgaca ggactataaa gataccaggc gtttccccct ggaagctccc
     5161 tcgtgcgctc tcctgttccg accctgccgc ttaccggata cctgtccgcc tttctccctt
     5221 cgggaagcgt ggcgctttct catagctcac gctgtaggta tctcagttcg gtgtaggtcg
     5281 ttcgctccaa gctgggctgt gtgcacgaac cccccgttca gcccgaccgc tgcgccttat
     5341 ccggtaacta tcgtcttgag tccaacccgg taagacacga cttatcgcca ctggcagcag
     5401 ccactggtaa caggattagc agagcgaggt atgtaggcgg tgctacagag ttcttgaagt
     5461 ggtggcctaa ctacggctac actagaagga cagtatttgg tatctgcgct ctgctgaagc
     5521 cagttacctt cggaaaaaga gttggtagct cttgatccgg caaacaaacc accgctggta
     5581 gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag
     5641 atcctttgat cttttctacg gggtctgacg ctcagtggaa cgaaaactca cgttaaggga
     5701 ttttggtcat gagattatca aaaaggatct tcacctagat ccttttaaat taaaaatgaa
     5761 gttttaaatc aatctaaagt atatatgagt aaacttggtc tgacagttac caatgcttaa
     5821 tcagtgaggc acctatctca gcgatctgtc tatttcgttc atccatagtt gcctgactcc
     5881 ccgtcgtgta gataactacg atacgggagg gcttaccatc tggccccagt gctgcaatga
     5941 taccgcgaga cccacgctca ccggctccag atttatcagc aataaaccag ccagccggaa
     6001 gggccgagcg cagaagtggt cctgcaactt tatccgcctc catccagtct attaattgtt
     6061 gccgggaagc tagagtaagt agttcgccag ttaatagttt gcgcaacgtt gttgccattg
     6121 ctacaggcat cgtggtgtca cgctcgtcgt ttggtatggc ttcattcagc tccggttccc
     6181 aacgatcaag gcgagttaca tgatccccca tgttgtgcaa aaaagcggtt agctccttcg
     6241 gtcctccgat cgttgtcaga agtaagttgg ccgcagtgtt atcactcatg gttatggcag
     6301 cactgcataa ttctcttact gtcatgccat ccgtaagatg cttttctgtg actggtgagt
     6361 actcaaccaa gtcattctga gaatagtgta tgcggcgacc gagttgctct tgcccggcgt
     6421 caatacggga taataccgcg ccacatagca gaactttaaa agtgctcatc attggaaaac
     6481 gttcttcggg gcgaaaactc tcaaggatct taccgctgtt gagatccagt tcgatgtaac
     6541 ccactcgtgc acccaactga tcttcagcat cttttacttt caccagcgtt tctgggtgag
     6601 caaaaacagg aaggcaaaat gccgcaaaaa agggaataag ggcgacacgg aaatgttgaa
     6661 tactcatact cttccttttt caatattatt gaagcattta tcagggttat tgtctcatga
     6721 gcggatacat atttgaatgt atttagaaaa ataaacaaat aggggttccg cgcacatttc
     6781 cccgaaaagt gccacctgac gtctaagaaa ccattattat catgacatta acctataaaa
     6841 ataggcgtat cacgaggccc tttcgtcttc aagaattaac tgtgggaata ctcaggtatc
     6901 gtaagatgca agagttcgaa tctcttagca accattattt ttttcctcaa cataacgaga
     6961 acacacaggg gcgctatcgc acagaatcaa attcgatgac tggaaatttt ttgttaattt
     7021 cagaggtcgc ctgacgcata tacctttttc aactgaaaaa ttgggagaaa aaggaaaggt
     7081 gagaggccgg aaccggcttt tcatatagaa tagagaagcg ttcatgacta aatgcttgca
     7141 tcacaatact tgaagttgac aatattattt aaggacctat tgttttttcc aataggtggt
     7201 tagcaatcgt cttactttct aacttttctt accttttaca tttcagcaat atatatatat
     7261 atttcaagga tataccattc taatgtctgc ccctatgtct gcccctaaga agatcgtcgt
     7321 tttgccaggt gaccacgttg gtcaagaaat cacagccgaa gccattaagg ttcttaaagc
     7381 tatttctgat gttcgttcca atgtcaagtt cgatttcgaa aatcatttaa ttggtggtgc
     7441 tgctatcgat gctacaggtg tcccacttcc agatgaggcg ctggaagcct ccaagaaggt
     7501 tgatgccgtt ttgttaggtg ctgtgggtgg tcctaaatgg ggtacaggta gtgttagacc
     7561 tgaacaaggt ttactaaaaa tccgtaaaga acttcaattg tacgccaact taagaccatg
     7621 taactttgca tccgactctc ttttagactt atctccaatc aagccacaat ttgctaaagg
     7681 tactgacttc gttgttgtca gagaattagt gggaggtatt tactttggta agagaaagga
     7741 agacgatggt gatggtgtcg cttgggatag tgaacaatac accgttccag aagtgcaaag
     7801 aatcacaaga atggccgctt tcatggccct acaacatgag ccaccattgc ctatttggtc
     7861 cttggataaa gctaatgttt tggcctcttc aagattatgg agaaaaactg tggaggaaac
     7921 catcaagaac gaatttccta cattgaaggt tcaacatcaa ttgattgatt ctgccgccat
     7981 gatcctagtt aagaacccaa cccacctaaa tggtattata atcaccagca acatgtttgg
     8041 tgatatcatc tccgatgaag cctccgttat cccaggttcc ttgggtttgt tgccatctgc
     8101 gtccttggcc tctttgccag acaagaacac cgcatttggt ttgtacgaac catgccacgg
     8161 ttctgctcca gatttgccaa agaataaggt tgaccctatc gccactatct tgtctgctgc
     8221 aatgatgttg aaattgtcat tgaacttgcc tgaagaaggt aaggccattg aagatgcagt
     8281 taaaaaggtt ttggatgcag gtatcagaac tggtgattta ggtggttcca acagtaccac
     8341 cgaagtcggt gatgctgtcg ccgaagaagt taagaaaatc cttgcttaaa aagattctct
     8401 ttttttatga tatttgtaca taaactttat aaatgaaatt cataatagaa acgacacgaa
     8461 attacaaaat ggaatatgtt catagggtag aattaattct catgtttgac agcttatcat
     8521 cgatcgtcca actgcatgga gatgagtcgt ggcaagaata ccaagagttc ctcggtttgc
     8581 cagttattaa aagactcgta tttccaaaag actgcaacat actactcagt gcagcttcac
     8641 agaaacctca ttcgtttatt cccttgtttg attcagaagc aggtgggaca ggtgaacttt
     8701 tggattggaa ctcgatttct gactgggttg gaaggcaaga gagccccgag agcttacatt
     8761 ttatgttagc tggtggactg acgccagaaa atgttggtga tgcgcttaga ttaaatggcg
     8821 ttattggtgt tgatgtaagc ggaggtgtgg agacaaatgg tgtaaaagac tctaacaaaa
     8881 tagcaaattt cgtcaaaaat gctaagaaat aggttattac tgagtagtat ttatttaagt
     8941 attgtttgtg cacttgcctg caagcctttt gaaaagcaag cataaaagat ctaaacataa
     9001 aatctgtaaa ataacaagat gtaaagataa tgctaaatca tttggctttt tgattgattg
     9061 tacaggaaaa tatacatcgc agggggttga cttttaccat ttcaccgcaa tggaatcaaa
     9121 cttgttgaag agaatgttca caggcgcata cgctacaatg acccgattct tgctagcctt
     9181 ttctcggtct tgcaaacaac cgccggcagc ttagtatata aatacacatg tacatacctc
     9241 tctccgtatc ctcgtaatca ttttcttgta tttatcgtct tttcgctgta aaaactttat
     9301 cacacttatc tcaaatacac ttattaaccg cttttactat tatcttctac gctgacagta
     9361 atatcaaaca gtgacacata ttaaacacag tggtttcttt gcataaacac catcagcctc
     9421 aagtcgtcaa gtaaagattt cgtgttcatg cagatagata acaatctata tgttgataat
     9481 tagcgttgcc tcatcaatgc gagatccgtt taaccggacc ctagtgcact taccccacgt
     9541 tcggtccact gtgtgccgaa catgctcctt cactatttta acatgtggaa ttaattctca
     9601 tgtttgacag cttatcatcg aactctaaga ggtgatactt atttactgta aaactgtgac
     9661 gataaaaccg gaaggaagaa taagaaaact cgaactgatc tataatgcct attttctgta
     9721 aagagtttaa gctatgaaag cctcggcatt ttggccgctc ctaggtagtg ctttttttcc
     9781 aaggacaaaa cagtttcttt ttcttgagca ggttttatgt ttcggtaatc ataaacaata
     9841 aataaattat ttcatttatg tttaaaaata aaaaataaaa aagtatttta aatttttaaa
     9901 aaagttgatt ataagcatgt gaccttttgc aagcaattaa attttgcaat ttgtgatttt
     9961 aggcaaaagt tacaatttct ggctcgtgta atatatgtat gctaaagtga acttttacaa
    10021 agtcgatatg gacttagtca aaagaaattt tcttaaaaat atatagcact agccaattta
    10081 gcacttcttt atgagatata ttatagactt tattaagcca gatttgtgta ttatatgtat
    10141 ttacccggcg aatcatggac atacattctg aaataggtaa tattctctat ggtgagacag
    10201 catagataac ctaggataca agttaaaagc tagtactgtt ttgcagtaat ttttttcttt
    10261 tttataagaa tgttaccacc taaataagtt ataaagtcaa tagttaagtt tgatatttga
    10321 ttgtaaaata ccgtaatata tttgcatgat caaaaggctc aatgttgact agccagcatg
    10381 tcaaccacta tattgatcac cgatatatgg acttccacac caactagtaa tatgacaata
    10441 aattcaagat attcttcatg agaatggccc agcgatatat gcggtgtgaa ataccgcaca
    10501 gatgcgtaag gagaaaatac cgcatcaggc gccattcgcc attcaggctg cgcaactgtt
    10561 gggaagggcg atcggtgcgg gcctcttcgc tattacgcca gggatacaag ttaaaagcta
    10621 gtactgtttt gcagtaattt ttttcttttt tataagaatg ttaccaccta aataagttat
    10681 aaagtcaata gttaagttt
//
