LOCUS       GU931385                9842 bp    DNA     circular SYN 20-JUN-2012
DEFINITION  Overexpression vector pWALIUM10-moe, complete sequence.
ACCESSION   GU931385
VERSION     GU931385.2  GI:392347481
KEYWORDS    .
SOURCE      Overexpression vector pWALIUM10-moe
  ORGANISM  Overexpression vector pWALIUM10-moe
            other sequences; artificial sequences; vectors.
REFERENCE   1  (bases 1 to 9842)
  AUTHORS   Ni,J.-Q., Liu,L.-P., Perkins,L.A. and Perrimon,N.
  TITLE     Vectors for transgenic flies
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 9842)
  AUTHORS   Ni,J.-Q., Liu,L.-P., Perkins,L.A. and Perrimon,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2010) Genetics, Harvard Medical School, 77 Avenue
            Louis Pasteur, Boston, MA 02115, USA
REFERENCE   3  (bases 1 to 9842)
  AUTHORS   Hu,Y. and Perkins,L.A.
  TITLE     Direct Submission
  JOURNAL   Submitted (20-JUN-2012) Genetics, Harvard Medical School, 77 Avenue
            Louis Pasteur, Boston, MA 02115, USA
  REMARK    Sequence update by submitter
COMMENT     On Jun 20, 2012 this sequence version replaced gi:291501163.
FEATURES             Location/Qualifiers
     source          join(1..6263,7252..10807)
                     /dnas_title="pWALIUM10-V5-BirA12trunc-NES"
                     /organism="Overexpression vector pWALIUM10-moe"
                     /mol_type="other DNA"
                     /db_xref="taxon:747297"
                     /lab_host="Drosophila melanogaster"
     misc_feature    682..4813
                     /note="white"
     CDS             complement(join(1449..2063,2134..2265,2486..2801,
                     2863..3517,3592..3865,4260..4331))
                     /dnas_title="white"
                     /note="selectable marker"
                     /codon_start=1
                     /transl_table=11
                     /product="white"
                     /protein_id="ADE08347.1"
                     /db_xref="GI:291501164"
                     /translation="MGQEDQELLIRGGSKHPSAEHLNNGDSGAASQSCINQGFGQAKN
                     YGTLLPPSPPEDSGSGSGQLAENLTYAWHNMDIFGAVNQPGSGWRQLVNRTRGLFCNE
                     RHIPAPRKHLLKNVCGVAYPGELLAVMGSSGAGKTTLLNALAFRSPQGIQVSPSGMRL
                     LNGQPVDAKEMQARCAYVQQDDLFIGSLTAREHLIFQAMVRMPRHLTYRQRVARVDQV
                     IQELSLSKCQHTIIGVPGRVKGLSGGERKRLAFASEALTDPPLLICDEPTSGLDSFTA
                     HSVVQVLKKLSQKGKTVILTIHQPSSELFELFDKILLMAEGRVAFLGTPSEAVDFFSY
                     VGAQCPTNYNPADFYVQVLAVVPGREIESRDRIAKICDNFAISKVARDMEQLLATKNL
                     EKPLEQPENGYTYKATWFMQFRAVLWRSWLSVLKEPLLVKVRLIQTTMVAILIGLIFL
                     GQQLTQVGVMNINGAIFLFLTNMTFQNVFATINVFTSELPVFMREARSRLYRCDTYFL
                     GKTIAELPLFLTVPLVFTAIAYPMIGLRAGVLHFFNCLALVTLVANVSTSFGYLISCA
                     SSSTSMALSVGPPVIIPFLLFGGFFLNSGSVPVYLKWLSYLSWFRYANEGLLINQWAD
                     VEPGEISCTSSNTTCPSSGKVILETLNFSAADLPLDYVGLAILIVSFRVLAYLALRLR
                     ARRKE"
     intron          3518..3591
                     /note="white intron"
     misc_recomb     4856..5223
                     /note="attB docking site"
     misc_feature    5235..5665
                     /note="gypsy insulator"
     misc_recomb     5683..5716
                     /note="LoxP"
     misc_feature    5723..5832
                     /note="5XUAS"
     misc_recomb     5839..5872
                     /note="LoxP"
     misc_feature    5879..5988
                     /note="5XUAS"
     regulatory      5995..6254
                     /regulatory_class="promoter"
                     /note="Hsp70 promoter"
     misc_feature    join(6255..6263,7252..7256)
                     /note="multiple cloning site"
     intron          7297..7443
                     /note="ftz intron"
     misc_feature    7467..8166
                     /note="SV40 polyA region"
     misc_feature    8173..8603
                     /note="gypsy insulator"
     misc_feature    9825..10484
                     /note="AmpR"
     insert          6264..7251
                     /source="pValium10-roe"
                     /type="Custom cloned insert"
     misc_recomb     6283..7239
                     /note="attR; gateway recombination"
     misc_feature    7253..7256
                     /note="delete and replace with in-frame cds for
                     C-terminal fusion using Gibson"
     misc_feature    6295..7228
                     /note="paste into expression clone"
     misc_feature    6328..6331
                     /note="delete and replace with CACC + gene"
     misc_feature    6335..6376
                     /label=V5 tag
                     /note="V5 tag"
     misc_feature    6377..6382
                     /label=NheI
                     /note="NheI"
     CDS             6383..7153
                     /codon_start=1
                     /note="BirA (humanized)"
                     /translation="IPLLNAKQILGQLDGGSVAVLPVVDSTNQYLLDRIGELKSGDAC
                     IAEYQQAGRGSRGRKWFSPFGANLYLSMFWRLKRGPAAIGLGPVIGIVMAEALRKLGA
                     DKVRVKWPNDLYLQDRKLAGILVELAGITGDAAQIVIGAGINVAMRRVEESVVNQGWI
                     TLQEAGINLDRNTLAAMLIRELRAALELFEQEGLAPYLSRWEKLDNFINRPVKLIIGD
                     KEIFGISRGIDKQGALLLEQDGVIKPWMGGEISLRSAEK"
     misc_feature    6386..6388
                     /label=Q65P
                     /note="Q65P"
     misc_feature    6452..6454
                     /note="I87V"
     misc_feature    6536..6538
                     /label=G115G
                     /note="G115G"
     misc_feature    6545..6547
                     /label=R118S
                     /note="R118S"
     misc_feature    6611..6613
                     /note="E141K"
     misc_difference 6614..6616
                     /note="Q142R"
     misc_feature    6638..6640
                     /note="S150G"
     misc_feature    6641..6643
                     /label=L151P
                     /note="L151P"
     misc_feature    6668..6670
                     /note="V160A"
     misc_feature    6764..6766
                     /note="T192A"
     misc_feature    6770..6772
                     /note="K194I"
     misc_feature    6815..6817
                     /note="M209V"
     misc_structure  7061..7063
                     /note="G291G"
     misc_feature    7103..7105
                     /label=I305V
                     /note="I305V"
     misc_feature    join(7154..7164,7166..7189)
                     /label=NES
                     /note="NES"
                     /dnas_title="NES"
     misc_feature    7190..7192
                     /label=Stop
                     /note="Stop"
     misc_feature    7193..7195
                     /label=STOP
                     /note="STOP"
     misc_feature    6295..6311
                     /note="attL1"
     misc_feature    7212..7228
                     /note="attL2"
ORIGIN      
        1 cacctaaatt gtaagcgtta atattttgtt aaaattcgcg ttaaattttt gttaaatcag
       61 ctcatttttt aaccaatagg ccgaaatcgg caaaatccct tataaatcaa aagaatagac
      121 cgagataggg ttgagtgttg ttccagtttg gaacaagagt ccactattaa agaacgtgga
      181 ctccaacgtc aaagggcgaa aaaccgtcta tcagggcgat ggcccactac gtgaaccatc
      241 accctaatca agttttttgg ggtcgaggtg ccgtaaagca ctaaatcgga accctaaagg
      301 gagcccccga tttagagctt gacggggaaa gccggcgaac gtggcgagaa aggaagggaa
      361 gaaagcgaaa ggagcgggcg ctagggcgct ggcaagtgta gcggtcacgc tgcgcgtaac
      421 caccacaccc gccgcgctta atgcgccgct acagggcgcg tcccattcgc cattcaggct
      481 gcgcaactgt tgggaagggc gatcggtgcg ggcctcttcg ctattacgcc agctggcgaa
      541 agggggatgt gctgcaaggc gattaagttg ggtaacgcca gggttttccc agtcacgacg
      601 ttgtaaaacg acggccagtg aattgtaata cgactcacta tagggcgaat tgggtacaag
      661 cttcacgacc tgaggcgcgc cCTAGTtcca gtgaaatcca agcattttct aaattaaatg
      721 tattcttatt attatagttg ttatttttga tatatataaa caacactatt atgcccacca
      781 tttttttgag atgcatctac acaaggaaca aacactggat gtcactttca gttcaaattg
      841 taacgctaat cactccgaac aggtcacaaa aaattacctt aaaaagtcat aatattaaat
      901 tagaataaat atagctgtga gggaaatata tacaaatata ttggagcaaa taaattgtac
      961 atacaaatat ttattactaa tttctattga gacgaaatga accactcgga accatttgag
     1021 cgaaccgaat cgcgcggaac taacgacagt cgctccaagg tcgtcgaaca aaaggtgaat
     1081 gtgttgcgga gagcgggtgg gagacagcga aagagcaact acgaaacgtg gtgtggtgga
     1141 ggtgaattat gaagagggcg cgcgatttga aaagtatgta tataaaaaat atatcccggt
     1201 gttttatgta gcgataaacg agtttttgat gtaaggtatg caggtgtgta agtcttttgg
     1261 ttagaagaca aatccaaagt ctacttgtgg ggatgttcga aggggaaata cttgtattct
     1321 ataggtcata tcttgttttt attggcacaa atataattac attagctttt tgagggggca
     1381 ataaacagta aacacgatgg taataatggt aaaaaaaaaa acaagcagtt atttcggata
     1441 tatgtcggct actccttgcg tcgggcccga agtcttagag ccagatatgc gagcacccgg
     1501 aagctcacga tgagaatggc cagacccacg tagtccagcg gcagatcggc ggcggagaag
     1561 ttaagcgtct ccaggatgac cttgcccgaa ctggggcacg tggtgttcga cgatgtgcag
     1621 ctaatttcgc ccggctccac gtccgcccat tggttaatca gcagaccctc gttggcgtaa
     1681 cggaaccatg agaggtacga caaccatttg aggtatactg gcaccgagcc cgagttcaag
     1741 aagaagccgc caaagagcag gaatggtatg ataaccggcg gacccacaga cagcgccatc
     1801 gaggtcgagg agctggcgca ggatattaga tatccgaagg acgttgacac attggccacc
     1861 agagtgacca gcgccaggca gttgaagaag tgcagcactc cggcccgcag tccgatcatc
     1921 ggataggcaa tcgccgtgaa gaccagtggc actgtgagaa aaagcggtaa ttcggcaatc
     1981 gttttgccca gaaagtatgt gtcacagcga taaagtcgac ttcgggcctc cctcataaaa
     2041 actggcagct ctgaggtgaa cacctaaatc gaatcgattc attagaaagt tagtaaatta
     2101 ttaatatgca aatgtattct aaacaagact tacatttatc gtggcaaaga cgttttgaaa
     2161 ggtcatgttg gtcaggaaga ggaagatggc tccgttgata ttcatcacgc ccacttgcgt
     2221 gagttgttgg cccaaaaaga tgaggccaat caagatggca accatctgca aattaaaatg
     2281 ttactcgcat ctcattaata ttcatatctt caacatgttc gcgagttaaa tgaaatttat
     2341 ttattttctg caaaactata aactatacat ctcattgaaa aaaactaaga agggtgtgga
     2401 atcaggcaat tctaactaaa atctagcgaa tttgtttcca agaattgtaa gcgttatatc
     2461 atttgtttcc actggaacca ctcaccgttg tctgaataag tcgcactttt acgaggagtg
     2521 gttccttgag caccgacagc caggatcgcc acaggaccgc ccggaactgc atgaaccagg
     2581 tggccttgta ggtgtaccca ttctccggct gctccagtgg cttctccaaa tttttggtgg
     2641 ccaacaactg ctccatatcc cgggctactt tgctaatagc aaaattgtcg catatcttgg
     2701 cgatccgatc acgggactcg atctcccgtc cgggcacaac ggccaacacc tgtacgtaaa
     2761 agtccgccgg attgtagttg gtaggacact gggcacccac gctggatagg agttgagatg
     2821 taatgtaatg ctagataccc ttaataaaca catcgaactc actaggaaaa gaagtcgacg
     2881 gcttcgctgg gagtgcccaa gaaagctacc ctgccctcgg ccatcagaag gatcttgtca
     2941 aagagctcaa acagctcgga agacggctga tgaatggtca ggatgacggt cttgcccttc
     3001 tgcgacagct tcttcagcac ctggacgacg ctgtgggcgg taaatgagtc cagtccggag
     3061 gtgggctcat cgcagatcag aagcggcgga tcggttagtg cctcggaggc gaatgccaga
     3121 cgcttccttt ctccgccgga cagacctttc accctgccgg gcacaccgat gatcgtgtgc
     3181 tgacatttgc tgagcgaaag ctcctggatc acctgatcca cgcgggccac tcgctgccga
     3241 taggtcagat gtcgtggcat ccgcaccatg gcctggaaaa tcaggtgttc cctggccgtt
     3301 agggagccga taaagaggtc atcctgctgg acataggcgc acctggcctg catctccttg
     3361 gcgtccacag gttggccatt gagcagtcgc atcccggatg gcgatacttg gatgccctgc
     3421 ggcgatcgaa aggcaagggc attcagcagg gtcgtctttc cggcaccgga actgcccatc
     3481 acggccaaaa gttcgcccgg ataggccacg ccgcaaactg agtttcaaat tggtaattgg
     3541 accctttatt aagatttcac acagatcagc cgactgcgaa tagaaactca ccgttcttga
     3601 gcaaatgttt cctgggcgcc ggtatgtgtc gctcgttgca gaatagtccg cgtgtccggt
     3661 tgaccagctg ccgccatccg gagcccggct gattgaccgc cccaaagatg tccatattgt
     3721 gccaggcata ggtgaggttc tcggctagtt ggccgctccc tgaaccggag tcctccggcg
     3781 gactgggtgg caggagcgtg ccgtagtttt tggcctgccc gaagccctgg ttaatgcagc
     3841 tctgcgaagc cgctccgctg tcaccctgca atgatagggg atctcaaata tcaactacaa
     3901 gcgttatgct catctaaccc cgaacaaaac gaagtatcct acgaagtagg tttatacttt
     3961 tatttatttt ttgtgcatct aggatcagct taaaatatct ggttgttata ttttttgtaa
     4021 aaaagaatgt agtcgaaaat gaatgccttt agatgtcttg atcatgatat gatcttaaaa
     4081 attgtcttat atagcgagca cagctaccag aataatctgt ttcgtgtcac tatttgtttg
     4141 tgcgattgcg gtttgggatt tttgtgggtc gcagttctca cgccgcagac aatttgatgt
     4201 tgcaatcgca gttcctatag atcaagtgaa cttaagatgt atgcacatgt actactcaca
     4261 ttgttcagat gctcggcaga tgggtgtttg ctgcctccgc gaattaatag ctcctgatcc
     4321 tcttggccca ttgccgggat ttttcacact ttcccctgct tacccaccca aaaccaatca
     4381 ccaccccaat cactcaaaaa acaaacaaaa ataagaagcg agaggagttt tggcacagca
     4441 ctttgtgttt aattgatggc gtaaaccgct tggagcttcg tcacgaaacc gctgacaaag
     4501 tgcaactgaa ggcggacatt gacgctaggt aacgctacaa acggtggcga aagagatagc
     4561 ggacgcagcg gcgaaagaga cggcgatatt tctgtggaca gagaaggagg caaacagcgc
     4621 tgactttgag tggaatgtca ttttgagtga gaggtaatcg aaagaacctg gtacttcaaa
     4681 tacccttgga tcgaagtaaa tttaaaactg atcagataag ttcaatgata tccagtgcag
     4741 taaaaaataa aaaaaaaaaa tgtttttttt tatctacttt ccgcaaaaat gggttttatt
     4801 aacttacata catggcgcgc cagatcgcaa gaagcttgat atcatcgatc tcgaggctgc
     4861 atccaacgcg ttgggagctc tccggatcaa ttcggcttca ggtaccgtcg acgatgtagg
     4921 tcacggtctc gaagccgcgg tgcgggtgcc agggcgtgcc cttgggctcc ccgggcgcgt
     4981 actccacctc acccatctgg tccatcatga tgaacgggtc gaggtggcgg tagttgatcc
     5041 cggcgaacgc gcggcgcacc gggaagccct cgccctcgaa accgctgggc gcggtggtca
     5101 cggtgagcac gggacgtgcg acggcgtcgg cgggtgcgga tacgcggggc agcgtcagcg
     5161 ggttctcgac ggtcacggcg ggcatgtcga caagccgaat tgatccacta gaaggcctaa
     5221 ttcggtacac tagttggcca cgtaataagt gtgcgttgaa tttattcgca aaaacattgc
     5281 atattttcgg caaagtaaaa ttttgttgca taccttatca aaaaataagt gctgcatact
     5341 ttttagagaa accaaataat tttttattgc atacccgttt ttaataaaat acattgcata
     5401 ccctctttta ataaaaaata ttgcatactt tgacgaaaca aattttcgtt gcatacccaa
     5461 taaaagatta ttatattgca tacccgtttt taataaaata cattgcatac cctcttttaa
     5521 taaaaaatat tgcatacgtt gacgaaacaa attttcgttg catacccaat aaaagattat
     5581 tatattgcat accttttctt gccataccat ttagccgatc aattgtgctc ggcaacagta
     5641 tatttgtggt gtgccaacca acaacactag tagtaccagc ttataacttc gtataatgta
     5701 tgctatacga agttatctgc aggcaggtcg gagtactgtc ctccgagcgg agtactgtcc
     5761 tccgagcgga gtactgtcct ccgagcggag tactgtcctc cgagcggagt actgtcctcc
     5821 gagcggagac tcccatggat aacttcgtat aatgtatgct atacgaagtt atggatccgc
     5881 aggtcggagt actgtcctcc gagcggagta ctgtcctccg agcggagtac tgtcctccga
     5941 gcggagtact gtcctccgag cggagtactg tcctccgagc ggagactcgt cgacagcgag
     6001 cgccggagta taaatagagg cgcttcgtct acggagcgac aattcaattc aaacaagcaa
     6061 agtgaacacg tcgctaagcg aaagctaagc aaataaacaa gcgcagctga acaagctaaa
     6121 caatctgcag taaagtgcaa gttaaagtga atcaattaaa agtaaccagc aaccaagtaa
     6181 atcaactgca actactgaaa tctgccaaga agtaattatt gaatacaaga agagaactct
     6241 gaatagggaa ttgggaattc Gcagatctgc ggccgcggct cgaatcacaa gtttGTACAA
     6301 AAAAGCAGGC TCCGCGGCCG CCCCCTTCAC Catgggcaag cccatcccca accccctgct
     6361 gggcctggac agcaccgcta gcatcccgct gctgaacgct aaacagattc tgggacagct
     6421 ggacggcggg agcgtggcag tcctgcctgt ggtcgactcc accaatcagt acctgctgga
     6481 tcgaatcggc gagctgaaga gtggggatgc ttgcattgca gaatatcagc aggcagggag
     6541 aggaagcaga gggaggaaat ggttctctcc ttttggagct aacctgtacc tgagtatgtt
     6601 ttggcgcctg aagcggggac cagcagcaat cggcctgggc ccggtcatcg gaattgtcat
     6661 ggcagaagcg ctgcgaaagc tgggagcaga caaggtgcga gtcaaatggc ccaatgacct
     6721 gtatctgcag gatagaaagc tggcaggcat cctggtggag ctggccggaa taacaggcga
     6781 tgctgcacag atcgtcattg gcgccgggat taacgtggct atgaggcgcg tggaggaaag
     6841 cgtggtcaat cagggctgga tcacactgca ggaagcaggg attaacctgg acaggaatac
     6901 tctggccgct atgctgatcc gagagctgcg ggcagccctg gaactgttcg agcaggaagg
     6961 cctggctcca tatctgtcac ggtgggagaa gctggataac ttcatcaata gacccgtgaa
     7021 gctgatcatt ggggacaaag agattttcgg gattagccgg gggattgata aacagggagc
     7081 cctgctgctg gaacaggacg gagttatcaa accctggatg ggcggagaaa tcagtctgcg
     7141 gtctgccgaa aagctgcagc tgcctcccct ggagcgcctg accctggact aatagAAGGG
     7201 TGGGCGCGCC GACCCAGCTT TCTTGTACaa agtggtgatt cgagggtacc tctagagcaa
     7261 actagttctg atctgctaga caattgttgg catcaggtag gcatcacaca cgattaacaa
     7321 cccctaaaaa tacactttga aaatattgaa aatatgtttt tgtatacatt tttgatattt
     7381 tcaaacaata cgcagttata aaactcatta gctaacccat tttttctttg cttatgctta
     7441 cagattgcaa agaactagag ccgcgggatc tttgtgaagg aaccttactt ctgtggtgtg
     7501 acataattgg acaaactacc tacagagatt taaagctcta aggtaaatat aaaattttta
     7561 agtgtataat gtgttaaact actgattcta attgtttgtg tattttagat tccaacctat
     7621 ggaactgatg aatgggagca gtggtggaat gcctttaatg aggaaaacct gttttgctca
     7681 gaagaaatgc catctagtga tgatgaggct actgctgact ctcaacattc tactcctcca
     7741 aaaaagaaga gaaaggtaga agaccccaag gactttcctt cagaattgct aagttttttg
     7801 agtcatgctg tgtttagtaa tagaactctt gcttgctttg ctatttacac cacaaaggaa
     7861 aaagctgcac tgctatacaa gaaaattatg gaaaaatatt tgatgtatag tgccttgact
     7921 agagatcata atcagccata ccacatttgt agaggtttta cttgctttaa aaaacctccc
     7981 acacctcccc ctgaacctga aacataaaat gaatggaatt gttgttgtta acttgtttat
     8041 tgcagcttat aatggttaca aataaagcaa tagcatcaca aatttcacaa ataaagcatt
     8101 tttttcactg cattctagtt gtggtttgtc caaactcatc aatgtatctt atcatgtctg
     8161 gttccagagc tctggccacg taataagtgt gcgttgaatt tattcgcaaa aacattgcat
     8221 attttcggca aagtaaaatt ttgttgcata ccttatcaaa aaataagtgc tgcatacttt
     8281 ttagagaaac caaataattt tttattgcat acccgttttt aataaaatac attgcatacc
     8341 ctcttttaat aaaaaatatt gcatactttg acgaaacaaa ttttcgttgc atacccaata
     8401 aaagattatt atattgcata cccgttttta ataaaataca ttgcataccc tcttttaata
     8461 aaaaatattg catacgttga cgaaacaaat tttcgttgca tacccaataa aagattatta
     8521 tattgcatac cttttcttgc cataccattt agccgatcaa ttgtgctcgg caacagtata
     8581 tttgtggtgt gccaaccaac aacgagctcc agcttttgtt ccctttagtg agggttaatt
     8641 tcgagcttgg cgtaatcatg gtcatagctg tttcctgtgt gaaattgtta tccgctcaca
     8701 attccacaca acatacgagc cggaagcata aagtgtaaag cctggggtgc ctaatgagtg
     8761 agctaactca cattaattgc gttgcgctca ctgcccgctt tccagtcggg aaacctgtcg
     8821 tgccagctgc attaatgaat cggccaacgc gcggggagag gcggtttgcg tattgggcgc
     8881 tcttccgctt cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta
     8941 tcagctcact caaaggcggt aatacggtta tccacagaat caggggataa cgcaggaaag
     9001 aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg
     9061 tttttccata ggctccgccc ccctgacgag catcacaaaa atcgacgctc aagtcagagg
     9121 tggcgaaacc cgacaggact ataaagatac caggcgtttc cccctggaag ctccctcgtg
     9181 cgctctcctg ttccgaccct gccgcttacc ggatacctgt ccgcctttct cccttcggga
     9241 agcgtggcgc tttctcatag ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc
     9301 tccaagctgg gctgtgtgca cgaacccccc gttcagcccg accgctgcgc cttatccggt
     9361 aactatcgtc ttgagtccaa cccggtaaga cacgacttat cgccactggc agcagccact
     9421 ggtaacagga ttagcagagc gaggtatgta ggcggtgcta cagagttctt gaagtggtgg
     9481 cctaactacg gctacactag aaggacagta tttggtatct gcgctctgct gaagccagtt
     9541 accttcggaa aaagagttgg tagctcttga tccggcaaac aaaccaccgc tggtagcggt
     9601 ggtttttttg tttgcaagca gcagattacg cgcagaaaaa aaggatctca agaagatcct
     9661 ttgatctttt ctacggggtc tgacgctcag tggaacgaaa actcacgtta agggattttg
     9721 gtcatgagat tatcaaaaag gatcttcacc tagatccttt taaattaaaa atgaagtttt
     9781 aaatcaatct aaagtatata tgagtaaact tggtctgaca gttaccaatg cttaatcagt
     9841 gaggcaccta tctcagcgat ctgtctattt cgttcatcca tagttgcctg actccccgtc
     9901 gtgtagataa ctacgatacg ggagggctta ccatctggcc ccagtgctgc aatgataccg
     9961 cgagacccac gctcaccggc tccagattta tcagcaataa accagccagc cggaagggcc
    10021 gagcgcagaa gtggtcctgc aactttatcc gcctccatcc agtctattaa ttgttgccgg
    10081 gaagctagag taagtagttc gccagttaat agtttgcgca acgttgttgc cattgctaca
    10141 ggcatcgtgg tgtcacgctc gtcgtttggt atggcttcat tcagctccgg ttcccaacga
    10201 tcaaggcgag ttacatgatc ccccatgttg tgcaaaaaag cggttagctc cttcggtcct
    10261 ccgatcgttg tcagaagtaa gttggccgca gtgttatcac tcatggttat ggcagcactg
    10321 cataattctc ttactgtcat gccatccgta agatgctttt ctgtgactgg tgagtactca
    10381 accaagtcat tctgagaata gtgtatgcgg cgaccgagtt gctcttgccc ggcgtcaata
    10441 cgggataata ccgcgccaca tagcagaact ttaaaagtgc tcatcattgg aaaacgttct
    10501 tcggggcgaa aactctcaag gatcttaccg ctgttgagat ccagttcgat gtaacccact
    10561 cgtgcaccca actgatcttc agcatctttt actttcacca gcgtttctgg gtgagcaaaa
    10621 acaggaaggc aaaatgccgc aaaaaaggga ataagggcga cacggaaatg ttgaatactc
    10681 atactcttcc tttttcaata ttattgaagc atttatcagg gttattgtct catgagcgga
    10741 tacatatttg aatgtattta gaaaaataaa caaatagggg ttccgcgcac atttccccga
    10801 aaagtgc
//

