LOCUS       KC544267                4240 bp    DNA     circular SYN 05-MAR-2013
DEFINITION  Expression vector pBSPPc, complete sequence.
ACCESSION   KC544267
VERSION     KC544267.1  GI:455655635
KEYWORDS    .
SOURCE      Expression vector pBSPPc
  ORGANISM  Expression vector pBSPPc
            other sequences; artificial sequences; vectors.
REFERENCE   1  (bases 1 to 4240)
  AUTHORS   Xu,Y., Tao,F., Ma,C. and Xu,P.
  TITLE     New constitutive vectors: useful genetic engineering tools for
            biocatalysis
  JOURNAL   Appl. Environ. Microbiol. 79 (8), 2836-2840 (2013)
   PUBMED   23416993
REFERENCE   2  (bases 1 to 4240)
  AUTHORS   Xu,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2013) State Key Laboratory of Microbial
            Technology, Shandong University, South Shanda Road, Jinan, Shandong
            250100, China
FEATURES             Location/Qualifiers
     source          1..4240
                     /organism="Expression vector pBSPPc"
                     /mol_type="other DNA"
                     /db_xref="taxon:1298535"
     rep_origin      370..779
                     /note="ori region from pRO1600"
     gene            780..1613
                     /gene="rep"
     CDS             780..1613
                     /gene="rep"
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein"
                     /protein_id="AGG35806.1"
                     /db_xref="GI:455655636"
                     /translation="MASPPMVYKSNALVEAAYRLSVQEQRIVLACISQVKRSEPVTDE
                     VMYSVTAEDIATMAGVPIESSYNQLKEAALRLKRREVRLTQEPNGKGKRPSVMITGWV
                     QTIIYREGEGRVELRFTKDMLPYLTELTKQFTKYALADVAKMDSTHAIRLYELLMQWD
                     SIGQREIEIDQLRKWFQLEGRYPSIKDFKLRVLDPAVTQINEHSPLQVEWAQRKTGRK
                     VTHLLFSFGPKKPAKAVGKAPAKRKAGKISDAEIAKQARPGETWEAARARLTQMPLDL
                     A"
     gene            1833..2057
                     /gene="Pc"
     regulatory      1833..2057
                     /regulatory_class="promoter"
                     /gene="Pc"
                     /note="constitutive promoter"
     rep_origin      complement(2589..3203)
                     /note="the pMB1 derived origin of replication"
     gene            complement(3363..4223)
                     /gene="bla"
     CDS             complement(3363..4223)
                     /gene="bla"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-lactamase"
                     /protein_id="AGG35807.1"
                     /db_xref="GI:455655637"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE
                     YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
                     DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
                     LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
                     EIGASLIKHW"
ORIGIN      
        1 attattgaag catttatcag ggttattgtc tcatgagcgg atacatattt gaatgtattt
       61 agaaaaataa acaaataggg gttccgcgca catttccccg aaaagtgcca cctgacgtct
      121 aagaaaccat tattatcatg acattaacct ataaaaatag gcgtatcacg aggccctttc
      181 gtctcgcgcg tttcggtgat gacggtgaaa acctctgaca catgcagctc ccggagacgg
      241 tcacagcttg tctgtaagcg gatgccggga gcagacaagc ccgtcagggc gcgtcagcgg
      301 gtgttggcgg gtgtcggggc tggcttaact atgcggcatc agagcagatt gtactgagag
      361 tgcaccatac ctctcaggcg ccgctggtgc cgctggttgg acgccaaggg tgaatccgcc
      421 tcgataccct gattactcgc ttcctgcgcc ctctcaggcg gcgatagggg actggtaaaa
      481 cggggattgc ccagacgcct cccccgcccc ttcaggggca caaatgcggc cccaacgggg
      541 ccacgtagtg gtgcgttttt tgcgtttcca cccttttctt ccttttccct tttaaacctt
      601 ttaggacgtc tacaggccac gtaatccgtg gcctgtagag tttaaaaagg gacggatttg
      661 ttgccattaa gggacggatt tgttgttaag aagggacgga tttgttgttg taaagggacg
      721 gatttgttgt attgtgggac gcagatacag tgtcccctta tacacaagga atgtcgaaca
      781 tggcctcacc cccaatggtt tacaaaagca atgccctggt cgaggccgcg tatcgcctca
      841 gtgttcagga acagcggatc gttctggcct gtattagcca ggtgaagagg agcgagcctg
      901 tcaccgatga agtgatgtat tcagtgacgg cggaggacat agcgacgatg gcgggtgtcc
      961 ctatcgaatc ttcctacaac cagctcaaag aagcggccct gcgcctgaaa cggcgggaag
     1021 tccggttaac ccaagagccc aatggcaagg ggaaaagacc gagtgtgatg attaccggct
     1081 gggtgcaaac aatcatctac cgggagggtg agggccgtgt agaactcagg ttcaccaaag
     1141 acatgctgcc gtacctgacg gaactcacca aacagttcac caaatacgcc ttggctgacg
     1201 tggccaagat ggacagcacc cacgcgatca ggctttacga gctgctcatg caatgggaca
     1261 gcatcggcca gcgcgaaata gaaattgacc agctgcgaaa gtggtttcaa ctggaaggcc
     1321 ggtatccctc gatcaaggac ttcaagttgc gagtgcttga tccagccgtg acgcagatca
     1381 acgagcacag cccgctacag gtggagtggg cgcagcgaaa gaccgggcgc aaggtcacac
     1441 atctgttgtt cagttttgga ccgaagaagc ccgccaaggc ggtgggtaag gccccagcga
     1501 agcgcaaggc cgggaagatt tcagatgctg agatcgcgaa acaggctcgc cctggtgaga
     1561 catgggaagc ggcccgcgct cgactaaccc agatgccgct ggatctggcc tagaggccgt
     1621 ggccaccacg gcccggcctg cctttcaggc tgcgcaactg ttgggaaggg cgatcggtgc
     1681 gggcctcttc gctattacgc cagctggcga aagggggatg tgctgcaagg cgattaagtt
     1741 gggtaacgcc agggttttcc cagtcacgac gttgtaaaac gacggccagt gagcgcgcgt
     1801 aatacgactc actatagggc gaattgggta cctgccgata caagaacaac agcgcgttga
     1861 gcgcctgccg gtgggtggcc ggcgccactt gcttctcggt ggcgagcatg gtcagaaaac
     1921 cctcgacttc agcttgcccc atttcgcgcg gatgtcgaaa cccaccatgg ctgcgggccg
     1981 tccacaacac aaatgccttg gcccagtaga cataagcctt ctcggtctgt aggctgtaat
     2041 gcaggtagcg aacccgtctc gaggtcgacg gtatcgataa gcttgatatc gaattcctgc
     2101 agcccggggg atccactagt tctagagcgg ccgccaccgc ggtggagctc cagcttttgt
     2161 tccctttagt gagggttaat tgcgcgcttg gcgtaatcat ggtcatagct gtttcctgtg
     2221 tgaaattgtt atccgctcac aattccacac aacatacgag ccggaagcat aaagtgtaaa
     2281 gcctggggtg cctaatgagt gagctaactc acattaattg cgttgcgctc actgcccgct
     2341 ttccagtcgg gaaacctgtc gtgccagctg cattaatgaa tcggccaacg cgcggggaga
     2401 ggcggtttgc gtattgggcg ctcttccgct tcctcgctca ctgactcgct gcgctcggtc
     2461 gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg taatacggtt atccacagaa
     2521 tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt
     2581 aaaaaggccg cgttgctggc gtttttccat aggctccgcc cccctgacga gcatcacaaa
     2641 aatcgacgct caagtcagag gtggcgaaac ccgacaggac tataaagata ccaggcgttt
     2701 ccccctggaa gctccctcgt gcgctctcct gttccgaccc tgccgcttac cggatacctg
     2761 tccgcctttc tcccttcggg aagcgtggcg ctttctcata gctcacgctg taggtatctc
     2821 agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc
     2881 gaccgctgcg ccttatccgg taactatcgt cttgagtcca acccggtaag acacgactta
     2941 tcgccactgg cagcagccac tggtaacagg attagcagag cgaggtatgt aggcggtgct
     3001 acagagttct tgaagtggtg gcctaactac ggctacacta gaaggacagt atttggtatc
     3061 tgcgctctgc tgaagccagt taccttcgga aaaagagttg gtagctcttg atccggcaaa
     3121 caaaccaccg ctggtagcgg tggttttttt gtttgcaagc agcagattac gcgcagaaaa
     3181 aaaggatctc aagaagatcc tttgatcttt tctacggggt ctgacgctca gtggaacgaa
     3241 aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt
     3301 ttaaattaaa aatgaagttt taaatcaatc taaagtatat atgagtaaac ttggtctgac
     3361 agttaccaat gcttaatcag tgaggcacct atctcagcga tctgtctatt tcgttcatcc
     3421 atagttgcct gactccccgt cgtgtagata actacgatac gggagggctt accatctggc
     3481 cccagtgctg caatgatacc gcgagaccca cgctcaccgg ctccagattt atcagcaata
     3541 aaccagccag ccggaagggc cgagcgcaga agtggtcctg caactttatc cgcctccatc
     3601 cagtctatta attgttgccg ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc
     3661 aacgttgttg ccattgctac aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca
     3721 ttcagctccg gttcccaacg atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa
     3781 gcggttagct ccttcggtcc tccgatcgtt gtcagaagta agttggccgc agtgttatca
     3841 ctcatggtta tggcagcact gcataattct cttactgtca tgccatccgt aagatgcttt
     3901 tctgtgactg gtgagtactc aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt
     3961 tgctcttgcc cggcgtcaat acgggataat accgcgccac atagcagaac tttaaaagtg
     4021 ctcatcattg gaaaacgttc ttcggggcga aaactctcaa ggatcttacc gctgttgaga
     4081 tccagttcga tgtaacccac tcgtgcaccc aactgatctt cagcatcttt tactttcacc
     4141 agcgtttctg ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg aataagggcg
     4201 acacggaaat gttgaatact catactcttc ctttttcaat
//

