LOCUS       Exported                9666 bp ds-DNA     circular SYN 28-JUN-2019
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  recombinant plasmid
REFERENCE   1  (bases 1 to 9666)
  AUTHORS   NYU School of Medicine
  TITLE     Direct Submission
  JOURNAL   Exported Friday, Jun 28, 2019 from SnapGene 4.3.8.1
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..9666
                     /organism="recombinant plasmid"
                     /mol_type="other DNA"
     promoter        96..200
                     /gene="bla"
                     /label=AmpR promoter
     CDS             201..1061
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFFHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     215..234
                     /label=oTS108_Amp_fw
     primer_bind     complement(350..370)
                     /label=oTS109_Amp_rev
     rep_origin      1232..1820
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     promoter        2144..2174
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2182..2198
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     2206..2222
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        2243..2261
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        2309..2975
                     /gene="S. cerevisiae TDH3"
                     /label=GAP promoter
                     /note="promoter for glyceraldehyde-3-phosphate 
                     dehydrogenase"
     primer_bind     complement(2471..2491)
                     /label=oTS058_GPD_rev
     primer_bind     2708..2728
                     /label=oTS054_GPD_fw
     CDS             2988..2990
                     /codon_start=1
                     /label=START
                     /translation="M"
     CDS             3027..3053
                     /codon_start=1
                     /product="nuclear export signal from the HIV Rev protein 
                     (Fischer et al., 1995)"
                     /label=NES
                     /translation="LPPLERLTL"
     CDS             3054..3071
                     /codon_start=1
                     /label=2x GSA linker
                     /translation="GSAGSA"
     CDS             3072..3101
                     /codon_start=1
                     /product="Nuclear export signal from the protein kinase 
                     inhibitor (PKI) alpha (38-47)"
                     /label=NES (Wen et al. 1995)
                     /translation="LALKLAGLDI"
     CDS             3111..3818
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(3368..3387)
                     /label=oTS057_mCherry_rev
     primer_bind     3368..3387
                     /label=oTS055_mCherry_fw
     primer_bind     3736..3755
                     /label=oTS056_mCherry_fw_2
     CDS             3867..4136
                     /codon_start=1
                     /product="human Small ubiquitin-related modifier 3 (SUMO3) 
                     (1-90)"
                     /label=SUMO3
                     /translation="MSEEKPKEGVKTENDHINLKVAGQDGSVVQFKIKRHTPLSKLMKA
                     YSERQGLSMRQIRFRFDGQPINETDTPAQLEMEDEDTIDVFQQQT"
     CDS             4137..4178
                     /codon_start=1
                     /label=VV2GGS linker
                     /translation="VVGGSGGSGGSGGS"
     CDS             4179..4448
                     /codon_start=1
                     /product="human Small ubiquitin-related modifier 3 (SUMO3) 
                     (1-90)"
                     /label=SUMO3
                     /translation="MSEEKPKEGVKTENDHINLKVAGQDGSVVQFKIKRHTPLSKLMKA
                     YSERQGLSMRQIRFRFDGQPINETDTPAQLEMEDEDTIDVFQQQT"
     CDS             4449..4490
                     /codon_start=1
                     /label=VV2GGS linker
                     /translation="VVGGSGGSGGSGGS"
     CDS             4491..4760
                     /codon_start=1
                     /product="human Small ubiquitin-related modifier 3 (SUMO3) 
                     (1-90)"
                     /label=SUMO3
                     /translation="MSEEKPKEGVKTENDHINLKVAGQDGSVVQFKIKRHTPLSKLMKA
                     YSERQGLSMRQIRFRFDGQPINETDTPAQLEMEDEDTIDVFQQQT"
     CDS             4761..4802
                     /codon_start=1
                     /label=VV2GGS linker
                     /translation="VVGGSGGSGGSGGS"
     CDS             4803..5072
                     /codon_start=1
                     /product="human Small ubiquitin-related modifier 3 (SUMO3) 
                     (1-90)"
                     /label=SUMO3
                     /translation="MSEEKPKEGVKTENDHINLKVAGQDGSVVQFKIKRHTPLSKLMKA
                     YSERQGLSMRQIRFRFDGQPINETDTPAQLEMEDEDTIDVFQQQT"
     CDS             5073..5114
                     /codon_start=1
                     /label=VV2GGS linker
                     /translation="VVGGSGGSGGSGGS"
     CDS             5115..5384
                     /codon_start=1
                     /product="human Small ubiquitin-related modifier 3 (SUMO3) 
                     (1-90)"
                     /label=SUMO3
                     /translation="MSEEKPKEGVKTENDHINLKVAGQDGSVVQFKIKRHTPLSKLMKA
                     YSERQGLSMRQIRFRFDGQPINETDTPAQLEMEDEDTIDVFQQQT"
     CDS             5385..5426
                     /codon_start=1
                     /label=VV2GSS linker
                     /translation="VVGSSGSSGSSGSS"
     CDS             5427..5696
                     /codon_start=1
                     /product="human Small ubiquitin-related modifier 3 (SUMO3) 
                     (1-90)"
                     /label=SUMO3
                     /translation="MSEEKPKEGVKTENDHINLKVAGQDGSVVQFKIKRHTPLSKLMKA
                     YSERQGLSMRQIRFRFDGQPINETDTPAQLEMEDEDTIDVFQQQT"
     CDS             5697..5738
                     /codon_start=1
                     /label=VV2GSS linker
                     /translation="VVGSSGSSGSSGSS"
     CDS             5745..6014
                     /codon_start=1
                     /product="human Small ubiquitin-related modifier 3 (SUMO3) 
                     (1-90)"
                     /label=SUMO3
                     /translation="MSEEKPKEGVKTENDHINLKVAGQDGSVVQFKIKRHTPLSKLMKA
                     YSERQGLSMRQIRFRFDGQPINETDTPAQLEMEDEDTIDVFQQQT"
     CDS             6015..6056
                     /codon_start=1
                     /label=VV2GSS linker
                     /translation="VVGSSGSSGSSGSS"
     CDS             6057..6326
                     /codon_start=1
                     /product="human Small ubiquitin-related modifier 3 (SUMO3) 
                     (1-90)"
                     /label=SUMO3
                     /translation="MSEEKPKEGVKTENDHINLKVAGQDGSVVQFKIKRHTPLSKLMKA
                     YSERQGLSMRQIRFRFDGQPINETDTPAQLEMEDEDTIDVFQQQT"
     CDS             6327..6368
                     /codon_start=1
                     /label=VV2GSS linker
                     /translation="VVGSSGSSGSSGSS"
     CDS             6375..6644
                     /codon_start=1
                     /product="human Small ubiquitin-related modifier 3 (SUMO3) 
                     (1-90)"
                     /label=SUMO3
                     /translation="MSEEKPKEGVKTENDHINLKVAGQDGSVVQFKIKRHTPLSKLMKA
                     YSERQGLSMRQIRFRFDGQPINETDTPAQLEMEDEDTIDVFQQQT"
     CDS             6645..6686
                     /codon_start=1
                     /label=VV2GSS linker
                     /translation="VVGSSGSSGSSGSS"
     CDS             6687..6956
                     /codon_start=1
                     /product="human Small ubiquitin-related modifier 3 (SUMO3) 
                     (1-90)"
                     /label=SUMO3
                     /translation="MSEEKPKEGVKTENDHINLKVAGQDGSVVQFKIKRHTPLSKLMKA
                     YSERQGLSMRQIRFRFDGQPINETDTPAQLEMEDEDTIDVFQQQT"
     CDS             6957..6992
                     /codon_start=1
                     /label=GSS linker
                     /translation="GSSGSSGSSGSS"
     CDS             6993..7061
                     /codon_start=1
                     /product="Sumo Interacting Motif"
                     /label=SIM
                     /translation="KVDVIDLTIESSSDEEEDPPAKR"
     CDS             7062..7097
                     /codon_start=1
                     /label=GSS linker
                     /translation="GSSGSSGSSGSS"
     CDS             7104..7172
                     /codon_start=1
                     /product="Sumo Interacting Motif"
                     /label=SIM
                     /translation="KVDVIDLTIESSSDEEEDPPAKR"
     CDS             7173..7208
                     /codon_start=1
                     /label=GGS linker
                     /translation="GGSGGSGGSGGS"
     CDS             7215..7283
                     /codon_start=1
                     /product="Sumo Interacting Motif"
                     /label=SIM
                     /translation="KVDVIDLTIESSSDEEEDPPAKR"
     CDS             7284..7319
                     /codon_start=1
                     /label=GSS linker
                     /translation="GSSGSSGSSGSS"
     CDS             7320..7388
                     /codon_start=1
                     /product="Sumo Interacting Motif"
                     /label=SIM
                     /translation="KVDVIDLTIESSSDEEEDPPAKR"
     CDS             7389..7424
                     /codon_start=1
                     /label=GGS linker
                     /translation="GGSGGSGGSGGS"
     CDS             7425..7493
                     /codon_start=1
                     /product="Sumo Interacting Motif"
                     /label=SIM
                     /translation="KVDVIDLTIESSSDEEEDPPAKR"
     CDS             7494..7529
                     /codon_start=1
                     /label=GGS linker
                     /translation="GGSGGSGGSGGS"
     CDS             7530..7598
                     /codon_start=1
                     /product="Sumo Interacting Motif"
                     /label=SIM
                     /translation="KVDVIDLTIESSSDEEEDPPAKR"
     promoter        complement(7683..7701)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(7708..7724)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     rep_origin      7869..8324
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     CDS             complement(8453..9256)
                     /codon_start=1
                     /gene="S. cerevisiae URA3"
                     /product="orotidine-5'-phosphate decarboxylase, required 
                     for uracil biosynthesis"
                     /label=URA3
                     /note="yeast auxotrophic marker, counterselectable with 
                     5-fluoroorotic acid (5-FOA)"
                     /translation="MSKATYKERAATHPSPVAAKLFNIMHEKQTNLCASLDVRTTKELL
                     ELVEALGPKICLLKTHVDILTDFSMEGTVKPLKALSAKYNFLLFEDRKFADIGNTVKLQ
                     YSAGVYRIAEWADITNAHGVVGPGIVSGLKQAAEEVTKEPRGLLMLAELSCKGSLSTGE
                     YTKGTVDIAKSDKDFVIGFIAQRDMGGRDEGYDWLIMTPGVGLDDKGDALGQQYRTVDD
                     VVSTGSDIIIVGRGLFAKGRDAKVEGERYRKAGWEAYLRRCGQQN"
     promoter        complement(9257..9472)
                     /gene="S. cerevisiae URA3"
                     /label=URA3 promoter
     primer_bind     complement(9508..9527)
                     /label=oTS100_backbone_fw
     primer_bind     9508..9527
                     /label=oTS065_backbone_rev
     primer_bind     9586..9605
                     /label=oTS068_knock-in_fw_comp
ORIGIN
        1 gacgaaaggg cctcgtgata cgcctatttt tataggttaa tgtcatgata ataatggttt
       61 cttagacgtc aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt
      121 tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat
      181 aatattgaaa aaggaagagt atgagtattc aacatttccg tgtcgccctt attccctttt
      241 ttgcggcatt ttgccttcct gtttttgctc acccagaaac gctggtgaaa gtaaaagatg
      301 ctgaagatca gttgggtgca cgagtgggtt acatcgaact ggatctcaac agcggtaaga
      361 tccttgagag ttttcgcccc gaagaacgtt ttccaatgat gagcactttt aaagttctgc
      421 tatgtggcgc ggtattatcc cgtattgacg ccgggcaaga gcaactcggt cgccgcatac
      481 actattctca gaatgacttg gttgagtact caccagtcac agaaaagcat cttacggatg
      541 gcatgacagt aagagaatta tgcagtgctg ccataaccat gagtgataac actgcggcca
      601 acttacttct gacaacgatc ggaggaccga aggagctaac cgcttttttt cacaacatgg
      661 gggatcatgt aactcgcctt gatcgttggg aaccggagct gaatgaagcc ataccaaacg
      721 acgagcgtga caccacgatg cctgtagcaa tggcaacaac gttgcgcaaa ctattaactg
      781 gcgaactact tactctagct tcccggcaac aattaataga ctggatggag gcggataaag
      841 ttgcaggacc acttctgcgc tcggcccttc cggctggctg gtttattgct gataaatctg
      901 gagccggtga gcgtgggtct cgcggtatca ttgcagcact ggggccagat ggtaagccct
      961 cccgtatcgt agttatctac acgacgggca gtcaggcaac tatggatgaa cgaaatagac
     1021 agatcgctga gataggtgcc tcactgatta agcattggta actgtcagac caagtttact
     1081 catatatact ttagattgat ttaaaacttc atttttaatt taaaaggatc taggtgaaga
     1141 tcctttttga taatctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt
     1201 cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct
     1261 gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc
     1321 taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgtcc
     1381 ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc
     1441 tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg
     1501 ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt
     1561 cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg
     1621 agcattgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg
     1681 gcagggtcgg aacaggagag cgcacgaggg agcttccagg ggggaacgcc tggtatcttt
     1741 atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag
     1801 gggggccgag cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt
     1861 gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgta
     1921 ttaccgcctt tgagtgagct gataccgctc gccgcagccg aacgaccgag cgcagcgagt
     1981 cagtgagcga ggaagcggaa gagcgcccaa tacgcaaacc gcctctcccc gcgcgttggc
     2041 cgattcatta atgcagctgg cacgacaggt ttcccgactg gaaagcgggc agtgagcgca
     2101 acgcaattaa tgtgagttac ctcactcatt aggcacccca ggctttacac tttatgcttc
     2161 cggctcctat gttgtgtgga attgtgagcg gataacaatt tcacacagga aacagctatg
     2221 accatgatta cgccaagctc ggaattaacc ctcactaaag ggaacaaaag ctgggtaccg
     2281 ggccccccct cgagtcagtt cgagtttatc attatcaata ctgccatttc aaagaatacg
     2341 taaataatta atagtagtga ttttcctaac tttatttagt caaaaaatta gccttttaat
     2401 tctgctgtaa cccgtacatg cccaaaatag ggggcgggtt acacagaata tataacatcg
     2461 taggtgtctg ggtgaacagt ttattcctgg catccactaa atataatgga gcccgctttt
     2521 taagctggca tccagaaaaa aaaagaatcc cagcaccaaa atattgtttt cttcaccaac
     2581 catcagttca taggtccatt ctcttagcgc aactacagag aacaggggca caaacaggca
     2641 aaaaacgggc acaacctcaa tggagtgatg caacctgcct ggagtaaatg atgacacaag
     2701 gcaattgacc cacgcatgta tctatctcat tttcttacac cttctattac cttctgctct
     2761 ctctgatttg gaaaaagctg aaaaaaaagg ttgaaaccag ttccctgaaa ttattcccct
     2821 acttgactaa taagtatata aagacggtag gtattgattg taattctgta aatctatttc
     2881 ttaaacttct taaattctac ttttatagtt agtctttttt ttagttttaa aacaccaaga
     2941 acttagtttc gaataaacac acataaacaa acaaattaat taaaaaaatg tctttgatca
     3001 caagtggagc gtcaggggca cttcagttgc caccgttgga aagactaact ctgggctccg
     3061 ctggatcagc tttagccttg aaattagcag gtcttgatat cctgatcact atggtgagca
     3121 agggcgagga ggacaacatg gccatcatca aggagttcat gcgcttcaag gtgcacatgg
     3181 agggctccgt gaacggccac gagttcgaga tcgagggcga gggcgagggc cgcccctacg
     3241 agggcaccca gaccgccaag ctgaaggtga ccaagggcgg ccccctgccc ttcgcctggg
     3301 acatcctgtc ccctcagttc atgtacggct ccaaggccta cgtgaagcac cccgccgaca
     3361 tccccgacta cttgaagctg tccttccccg agggcttcaa gtgggagcgc gtgatgaact
     3421 tcgaggacgg cggcgtggtg accgtgaccc aggactcctc cctgcaggac ggcgagttca
     3481 tctacaaggt gaagctgcgc ggcaccaact tcccctccga cggccccgta atgcagaaga
     3541 agaccatggg ctgggaggcc tcctccgagc ggatgtaccc cgaggacggc gccctgaagg
     3601 gcgagatcaa gcagaggctg aagctgaagg acggcggcca ctacgacgcc gaggtcaaga
     3661 ccacctacaa ggccaagaag cccgtgcagc tgcccggcgc ctacaacgtc aacatcaagc
     3721 tggacatcac ctcccacaac gaggactaca ccatcgtgga acagtacgag cgcgccgagg
     3781 gccgccactc caccggcggc atggacgagc tgtacaaggc ggccgcaacc ggttcatccg
     3841 gatcttcagg gagttctggt tcaagcatgt cagaagagaa gcctaaggag ggcgtgaaaa
     3901 ccgagaatga ccacattaac ttgaaggttg ctggacagga tgggtccgtg gttcagttca
     3961 aaattaaacg ccacacaccg ttgtcgaaac tgatgaaagc gtacagcgaa cgccagggtc
     4021 tttctatgcg tcagattcgc ttccgctttg atggtcaacc gattaatgaa acggacacgc
     4081 cggcacagct ggagatggag gatgaagaca ctatcgacgt atttcagcag cagaccgtcg
     4141 tagggggctc cggcggaagc ggggggtcgg gaggttccat gagcgaagag aaaccaaaag
     4201 aaggagttaa aaccgaaaac gaccatatca acctgaaggt cgccggtcag gacggctcag
     4261 tcgtgcagtt caagattaaa cgtcataccc cattaagtaa gctgatgaag gcgtatagtg
     4321 agcgccaagg cttgtcgatg cgccagattc gttttcgctt tgatggtcag ccaatcaacg
     4381 aaaccgacac cccggcgcag ctggaaatgg aagatgaaga taccatcgat gtgttccaac
     4441 agcaaacggt tgttggcgga agcggcggga gcggtggtag cgggggttct atgtccgaag
     4501 aaaaaccgaa agagggtgtg aagactgaaa acgaccacat taatctgaag gtggcgggcc
     4561 aggacggttc cgttgttcag tttaaaatca aacgtcacac cccgctgtcg aaactgatga
     4621 aagcctatag cgaacgtcaa gggctgagca tgcgtcaaat tcgttttcgc tttgatggtc
     4681 agccaatcaa cgaaaccgat acaccggcgc agctggagat ggaagacgag gacaccattg
     4741 acgtcttcca gcaacaaact gtagtgggtg gctccggtgg ctctggtggg tctggtggct
     4801 cgatgagtga ggaaaaaccc aaagaaggcg tcaagactga gaacgatcat attaacctga
     4861 aagtcgcggg tcaagatggt tcagtggtgc agttcaaaat taaacgccac accccgctgt
     4921 caaagctgat gaaagcctat agtgagcgtc aaggtctgtc gatgcgccag atccgctttc
     4981 gctttgatgg tcagccaatc aacgagacag acactccggc acaactggag atggaagatg
     5041 aagacaccat cgatgttttt caacagcaga ccgtggtggg agggagcggc ggtagcgggg
     5101 gatcaggtgg tagtatgtct gaggaaaagc cgaaggaagg ggtaaagacc gaaaatgatc
     5161 acattaatct gaaagttgcg ggccaagatg gtagtgtagt gcaatttaaa atcaagcgtc
     5221 acaccccgtt atctaaactg atgaaagcat atagcgaacg ccaaggcctg tctatgcgcc
     5281 agattcgctt tcgctttgac gggcaaccaa ttaatgaaac ggacaccccg gcacaactgg
     5341 aaatggaaga cgaggatact attgacgtgt ttcagcaaca gacagtcgta ggttcatccg
     5401 gatcttcagg gagttctggt tcaagcatga gcgaagagaa acctaaagaa ggagttaaaa
     5461 ccgaaaatga ccatatcaac ctgaaggtgg ccggccagga cggctcagtc gtgcagttca
     5521 agatcaaacg tcatacccca ttaagtaagc tgatgaaggc gtatagtgag cgccaaggct
     5581 tgtcgatgcg ccagattcgt ttccgctttg acggtcagcc aatcaacgaa accgacaccc
     5641 cggcgcagct ggaaatggaa gacgaagata ctatcgatgt gttccaacag caaacggtcg
     5701 taggatcttc aggatcaagc ggttcatctg gtagttccag atctatgtca gaagagaagc
     5761 ctaaggaagg cgtgaaaacc gagaatgacc acattaactt gaaggttgct ggacaggatg
     5821 ggtccgtggt tcagttcaag attaaacgcc atacaccgtt gtcgaaactg atgaaggcgt
     5881 acagcgaacg tcagggtctt tctatgcgtc agattcgctt ccgctttgat ggtcaaccga
     5941 ttaatgaaac ggacacgccg gcacagctgg agatggagga tgaggacact atcgacgtat
     6001 ttcagcaaca gaccgttgtt ggatcttcag gatcaagcgg ttcatctggt agttccatgt
     6061 ccgaagaaaa accgaaggag ggtgtgaaga ctgaaaacga ccatattaat ctgaaggtgg
     6121 cgggccagga cggttccgtt gttcagttta agatcaaacg ccacaccccg ctgtcgaaac
     6181 tgatgaaagc ctatagtgaa cgtcaagggc tgagcatgcg tcaaattcgt tttcgctttg
     6241 atggtcaacc aatcaacgaa accgatacac cggcgcagct ggaaatggaa gacgaggaca
     6301 ccattgacgt cttccagcaa caaactgttg ttggttcctc tggaagctca ggatctagtg
     6361 ggtcatcaac tagtatgagt gaagaaaaac ccaaggaagg cgtcaagact gagaacgatc
     6421 atattaacct gaaagtcgcg ggtcaagatg gttcagtggt gcagtttaaa attaaacgcc
     6481 acaccccgtt gtcaaagctg atgaaagcct atagtgaacg tcaaggtctg tcgatgcgcc
     6541 agatccgctt ccgctttgat ggtcagccaa tcaacgagac agacactccg gcacaactgg
     6601 agatggaaga tgaagacacc atcgatgttt tccaacagca gaccgttgtt ggttcatcag
     6661 gttcatccgg atctagcggg tcaagtatgt ctgaagaaaa gccgaaggaa ggggtaaaga
     6721 ccgaaaatga tcacattaat ctgaaagttg cgggccagga tggtagtgta gtgcaattta
     6781 aaattaaacg tcacaccccg ttgtctaaac tgatgaaagc atatagcgaa cgccaaggcc
     6841 tgtctatgcg ccagattcgc tttcgctttg acgggcaacc aatcaatgaa acggacaccc
     6901 cggcacaact ggaaatggaa gacgaggaca ctattgacgt gtttcagcaa cagacaggtt
     6961 catccggatc ttcagggagt tctggttcaa gcaaggttga tgtaatagat ttgaccatag
     7021 aatctagttc tgacgaggaa gaagatcctc ctgcaaagag gggatcttca ggatcaagcg
     7081 gttcatctgg tagttcctct agaaaggttg atgtaataga tttgaccata gaatctagtt
     7141 ctgacgagga agaagatcct cctgcaaaga ggggtggttc cgggggttca gggggttctg
     7201 gtggaagcgg atccaaggtt gatgtaatag atttgaccat agaatctagt tctgacgagg
     7261 aagaagatcc tcctgcaaag aggggttcct ctggaagctc aggatctagt gggtcatcaa
     7321 aggttgatgt aatagatttg accatagaat ctagttctga cgaggaagaa gatcctcctg
     7381 caaagagggg tggttccggg ggttcagggg gttctggtgg aagcaaggtt gatgtaatag
     7441 atttgaccat agaatctagt tctgacgagg aagaagatcc tcctgcaaag aggggtggtt
     7501 ccgggggttc agggggttct ggtggaagca aggttgatgt aatagatttg accatagaat
     7561 ctagttctga cgaggaagaa gatcctcctg caaagaggta aggcgcgcct ttttttttat
     7621 atatatatat atatatatat aactgaataa agagtatcat ctttcaaaga gctccaattc
     7681 gccctatagt gagtcgtatt acaattcact ggccgtcgtt ttacaacgtc gtgactggga
     7741 aaaccctggc gttacccaac ttaatcgcct tgcagcacat ccccccttcg ccagctggcg
     7801 taatagcgaa gaggcccgca ccgatcgccc ttcccaacag ttgcgcagcc tgaatggcga
     7861 atggcgcgac gcgccctgta gcggcgcatt aagcgcggcg ggtgtggtgg ttacgcgcag
     7921 cgtgaccgct acacttgcca gcgccctagc gcccgctcct ttcgctttct tcccttcctt
     7981 tctcgccacg ttcgccggct ttccccgtca agctctaaat cgggggctcc ctttagggtt
     8041 ccgatttagt gctttacggc acctcgaccc caaaaaactt gattagggtg atggttcacg
     8101 tagtgggcca tcgccctgat agacggtttt tcgccctttg acgttggagt ccacgttctt
     8161 taatagtgga ctcttgttcc aaactggaac aacactcaac cctatctcgg tctattcttt
     8221 tgatttataa gggattttgc cgatttcggc ctattggtta aaaaatgagc tgatttaaca
     8281 aaaatttaac gcgaatttta acaaaatatt aacgtttaca atttcctgat gcggtatttt
     8341 ctccttacgc atctgtgcgg tatttcacac cgcagggtaa taactgatat aattaaattg
     8401 aagctctaat ttgtgagttt agtatacatg catttactta taatacagtt ttttagtttt
     8461 gctggccgca tcttctcaaa tatgcttccc agcctgcttt tctgtaacgt tcaccctcta
     8521 ccttagcatc ccttcccttt gcaaatagtc ctcttccaac aataataatg tcagatcctg
     8581 tagagaccac atcatccacg gttctatact gttgacccaa tgcgtctccc ttgtcatcta
     8641 aacccacacc gggtgtcata atcaaccaat cgtaaccttc atctcttcca cccatgtctc
     8701 tttgagcaat aaagccgata acaaaatctt tgtcgctctt cgcaatgtca acagtaccct
     8761 tagtatattc tccagtagat agggagccct tgcatgacaa ttctgctaac atcaaaaggc
     8821 ctctaggttc ctttgttact tcttctgccg cctgcttcaa accgctaaca atacctgggc
     8881 ccaccacacc gtgtgcattc gtaatgtctg cccattctgc tattctgtat acacccgcag
     8941 agtactgcaa tttgactgta ttaccaatgt cagcaaattt tctgtcttcg aagagtaaaa
     9001 aattgtactt ggcggataat gcctttagcg gcttaactgt gccctccatg gaaaaatcag
     9061 tcaagatatc cacatgtgtt tttagtaaac aaattttggg acctaatgct tcaactaact
     9121 ccagtaattc cttggtggta cgaacatcca atgaagcaca caagtttgtt tgcttttcgt
     9181 gcatgatatt aaatagcttg gcagcaacag gactaggatg agtagcagca cgttccttat
     9241 atgtagcttt cgacatgatt tatcttcgtt tcctgcaggt ttttgttctg tgcagttggg
     9301 ttaagaatac tgggcaattt catgtttctt caacactaca tatgcgtata tataccaatc
     9361 taagtctgtg ctccttcctt cgttcttcct tctgttcgga gattaccgaa tcaaaaaaat
     9421 ttcaaagaaa ccgaaatcaa aaaaaagaat aaaaaaaaaa tgatgaattg aattgaaaag
     9481 cgtggtgcac tctcagtaca atctgctctg atgccgcata gttaagccag ccccgacacc
     9541 cgccaacacc cgctgacgcg ccctgacggg cttgtctgct cccggcatcc gcttacagac
     9601 aagctgtgac cgtctccggg agctgcatgt gtcagaggtt ttcaccgtca tcaccgaaac
     9661 gcgcga
//
