LOCUS       pRS306_HISpromot        6268 bp ds-DNA     circular     06-APR-2015
DEFINITION  
ACCESSION   
VERSION     
SOURCE      
  ORGANISM  
COMMENT     URA3 genomic fragment 186 (acc)-1299 (tat), CDS 417-1220 lower case
COMMENT     Clone into pRS306: NotI, SacI digest
COMMENT     
COMMENT     ApEinfo:methylated:0
FEATURES             Location/Qualifiers
     primer_bind     complement(4006..4025)
                     /label=T3
                     /ApEinfo_fwdcolor=cyan
                     /ApEinfo_revcolor=green
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     CDS             2025..2227
                     /gene="ScHIS3-P"
                     /product="S.c HIS3 Promoter"
                     /label=S.c HIS3 Promoter
                     /ApEinfo_fwdcolor=#0aff26
                     /ApEinfo_revcolor=#cdff2d
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     CDS             join(3008..3702,3704..3721)
                     /gene="yEGFP"
                     /label=yEGFP
                     /ApEinfo_fwdcolor=#9abeff
                     /ApEinfo_revcolor=#cdff2d
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     primer_bind     1950..1967
                     /label=M13-fwd
                     /ApEinfo_fwdcolor=cyan
                     /ApEinfo_revcolor=green
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_signal     2186..2225
                     /label=primer homology
                     /ApEinfo_fwdcolor=pink
                     /ApEinfo_revcolor=pink
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    3724..3972
                     /gene="URA3T"
                     /product="ScURA3 Terminator"
                     /label=ScURA3 Terminator
                     /ApEinfo_fwdcolor=#ff9d10
                     /ApEinfo_revcolor=#ff141e
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     primer_bind     complement(4043..4063)
                     /label=M13-rev
                     /ApEinfo_fwdcolor=cyan
                     /ApEinfo_revcolor=green
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     primer_bind     1966..1993
                     /label=T7
                     /ApEinfo_fwdcolor=cyan
                     /ApEinfo_revcolor=green
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     rep_origin      complement(4431..5113)
                     /label=ColE1 origin
                     /ApEinfo_fwdcolor=gray50
                     /ApEinfo_revcolor=gray50
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     rep_origin      1361..1801
                     /label=F1 ori
                     /ApEinfo_fwdcolor=gray50
                     /ApEinfo_revcolor=gray50
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     rep_origin      complement(1351..1806)
                     /label=M13 origin
                     /ApEinfo_fwdcolor=gray50
                     /ApEinfo_revcolor=gray50
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     CDS             complement(1811..1879)
                     /label=LacZ alpha
                     /ApEinfo_fwdcolor=cornflower blue
                     /ApEinfo_revcolor=cornflower blue
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_binding    complement(4069..4091)
                     /label=LacO
                     /ApEinfo_fwdcolor=cornflower blue
                     /ApEinfo_revcolor=cornflower blue
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     CDS             complement(5211..5870)
                     /label=AmpR
                     /ApEinfo_fwdcolor=yellow
                     /ApEinfo_revcolor=yellow
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     gene            191..1297
                     /label=URA3
                     /ApEinfo_fwdcolor=#ff7669
                     /ApEinfo_revcolor=#ffe761
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    147..168
                     /label=URA3-F1
                     /ApEinfo_fwdcolor=#ff0c0c
                     /ApEinfo_revcolor=green
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    1309..1328
                     /label=URA3-R1
                     /ApEinfo_fwdcolor=#ff1031
                     /ApEinfo_revcolor=green
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    2001..2006
                     /label=SacI
                     /ApEinfo_fwdcolor=#ffa208
                     /ApEinfo_revcolor=green
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    3975..3980
                     /label=XhoI
                     /ApEinfo_fwdcolor=#ffb211
                     /ApEinfo_revcolor=green
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     CDS             join(2258..3702,3704..3721)
                     /gene="'2xGFP"
                     /label='2xGFP
                     /ApEinfo_fwdcolor=#9abeff
                     /ApEinfo_revcolor=#cdff2d
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    2017..2024
                     /label=NotI
                     /ApEinfo_fwdcolor=#ff9902
                     /ApEinfo_revcolor=green
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     CDS             2276..2986
                     /gene="GFP"
                     /product="GFP"
                     /label=GFP
                     /ApEinfo_fwdcolor=#9abeff
                     /ApEinfo_revcolor=#cdff2d
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    2228..2233
                     /label=SacI(1)
                     /ApEinfo_label=SacI
                     /ApEinfo_fwdcolor=#ff8f19
                     /ApEinfo_revcolor=green
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
ORIGIN
        1 TCGCGCGTTT CGGTGATGAC GGTGAAAACC TCTGACACAT GCAGCTCCCG GAGACGGTCA
       61 CAGCTTGTCT GTAAGCGGAT GCCGGGAGCA GACAAGCCCG TCAGGGCGCG TCAGCGGGTG
      121 TTGGCGGGTG TCGGGGCTGG CTTAACTATG CGGCATCAGA GCAGATTGTA CTGAGAGTGC
      181 ACCATaccAC AGCTTTTCAA TTCAATTCAT CATTTTTTTT TTATTCTTTT TTTTGATTTC
      241 GGTTTCTTTG AAATTTTTTT GATTCGGTAA TCTCCGAACA GAAGGAAGAA CGAAGGAAGG
      301 AGCACAGACT TAGATTGGTA TATATACGCA TATGTAGTGT TGAAGAAACA TGAAATTGCC
      361 CAGTATTCTT AACCCAACTG CACAGAACAA AAACCTGCAG GAAACGAAGA TAAATCatgt
      421 cgaaagctac atataaggaa cgtgctgcta ctcatcctag tcctgttgct gccaagctat
      481 ttaatatcat gcacgaaaag caaacaaact tgtgtgcttc attggatgtt cgtaccacca
      541 aggaattact ggagttagtt gaagcattag gtcccaaaat ttgtttacta aaaacacatg
      601 tggatatctt gactgatttt tccatggagg gcacagttaa gccgctaaag gcattatccg
      661 ccaagtacaa ttttttactc ttcgaagaca gaaaatttgc tgacattggt aatacagtca
      721 aattgcagta ctctgcgggt gtatacagaa tagcagaatg ggcagacatt acgaatgcac
      781 acggtgtggt gggcccaggt attgttagcg gtttgaagca ggcggcagaa gaagtaacaa
      841 aggaacctag aggccttttg atgttagcag aattgtcatg caagggctcc ctatctactg
      901 gagaatatac taagggtact gttgacattg cgaagagcga caaagatttt gttatcggct
      961 ttattgctca aagagacatg ggtggaagag atgaaggtta cgattggttg attatgacac
     1021 ccggtgtggg tttagatgac aagggagacg cattgggtca acagtataga accgtggatg
     1081 atgtggtctc tacaggatct gacattatta ttgttggaag aggactattt gcaaagggaa
     1141 gggatgctaa ggtagagggt gaacgttaca gaaaagcagg ctgggaagca tatttgagaa
     1201 gatgcggcca gcaaaactaa AAAACTGTAT TATAAGTAAA TGCATGTATA CTAAACTCAC
     1261 AAATTAGAGC TTCAATTTAA TTATATCAGT TATTACCCta tGCGGTGTGA AATACCGCAC
     1321 AGATGCGTAA GGAGAAAATA CCGCATCAGG AAATTGTAAA CGTTAATATT TTGTTAAAAT
     1381 TCGCGTTAAA TTTTTGTTAA ATCAGCTCAT TTTTTAACCA ATAGGCCGAA ATCGGCAAAA
     1441 TCCCTTATAA ATCAAAAGAA TAGACCGAGA TAGGGTTGAG TGTTGTTCCA GTTTGGAACA
     1501 AGAGTCCACT ATTAAAGAAC GTGGACTCCA ACGTCAAAGG GCGAAAAACC GTCTATCAGG
     1561 GCGATGGCCC ACTACGTGAA CCATCACCCT AATCAAGTTT TTTGGGGTCG AGGTGCCGTA
     1621 AAGCACTAAA TCGGAACCCT AAAGGGAGCC CCCGATTTAG AGCTTGACGG GGAAAGCCGG
     1681 CGAACGTGGC GAGAAAGGAA GGGAAGAAAG CGAAAGGAGC GGGCGCTAGG GCGCTGGCAA
     1741 GTGTAGCGGT CACGCTGCGC GTAACCACCA CACCCGCCGC GCTTAATGCG CCGCTACAGG
     1801 GCGCGTCGCG CCATTCGCCA TTCAGGCTGC GCAACTGTTG GGAAGGGCGA TCGGTGCGGG
     1861 CCTCTTCGCT ATTACGCCAG CTGGCGAAAG GGGGATGTGC TGCAAGGCGA TTAAGTTGGG
     1921 TAACGCCAGG GTTTTCCCAG TCACGACGTT GTAAAACGAC GGCCAGTGAA TTGTAATACG
     1981 ACTCACTATA GGGCGAATTG GAGctccacc gcggtggcgg ccgcATTACT CTTGGCCTCC
     2041 TCTAGTACac tctatatttt tttatgcctc ggtaatgatt ttcgtttttt ttttccacct
     2101 agcggatgac tctttttttt tcttagcgat tggcattatc acataatgaa ttatacatta
     2161 tataaagtaa tgtgatttct acgaagaata tactaaaaaa tgagcaggca agataaacga
     2221 aggcaaaGAG CTCggtaccc ggggatccac tagtatggcc agtgaattcg agctcagtaa
     2281 aggagaagaa cttttcactg gagttgtccc aattcttgtt gaattagatg gtgatgttaa
     2341 tgggcacaaa ttttctgtca gtggagaggg tgaaggtgat gcaacatacg gaaaacttac
     2401 ccttaaattt atttgcacta ctggaaaact acctgttcca tggccaacac ttgtcactac
     2461 tttcacttat ggtgttcaat gcttttcaag atacccagat catatgaaac ggcatgactt
     2521 tttcaagagt gccatgcccg aaggttatgt acaggaaaga actatatttt tcaaagatga
     2581 cgggaactac aagacacgtg ctgaagtcaa gtttgaaggt gatacccttg ttaatagaat
     2641 cgagttaaaa ggtattgatt ttaaagaaga tggaaacatt cttggacaca aattggaata
     2701 caactataac tcacacaatg tatacatcat ggcagacaaa caaaagaatg gaatcaaagt
     2761 taacttcaaa attagacaca acattgaaga tggaagcgtt caactagcag accattatca
     2821 acaaaatact ccaattggcg atggccctgt ccttttacca gacaaccatt acctgtccac
     2881 acaatctgcc ctttcgaaag atcccaacga aaagagagac cacatggtcc ttcttgagtt
     2941 tgtaacagct gctgggatta cacatggcat ggatgaacta tacaaaggag cgccgggagc
     3001 aggtgcatct aaaggtgaag aattattcac tggtgttgtc ccaattttgg ttgaattaga
     3061 tggtgatgtt aatggtcaca aattttctgt ctccggtgaa ggtgaaggtg atgctactta
     3121 cggtaaattg accttaaaat ttatttgtac tactggtaaa ttgccagttc catggccaac
     3181 cttagtcact actttaactt atggtgttca atgtttttct agatacccag atcatatgaa
     3241 acaacatgac tttttcaagt ctgccatgcc agaaggttat gttcaagaaa gaactatttt
     3301 tttcaaagat gacggtaact acaagaccag agctgaagtc aagtttgaag gtgatacctt
     3361 agttaataga atcgaattaa aaggtattga ttttaaagaa gatggtaaca ttttaggtca
     3421 caaattggaa tacaactata actctcacaa tgtttacatc atggctgaca aacaaaagaa
     3481 tggtatcaaa gttaacttca aaattagaca caacattgaa gatggttctg ttcaattagc
     3541 tgaccattat caacaaaata ctccaattgg tgatggtcca gtcttgttac cagacaacca
     3601 ttacttatcc actcaatctg ccttatccaa agatccaaac gaaaagagag accacatggt
     3661 cttgttagaa tttgttactg ctgctggtat tacccatggt atTgatgaat tgtacaaata
     3721 aggcgcgcca ttataagtaa atgcatgtat actaaactca caaattagag cttcaattta
     3781 attatatcag ttattacccg ggaatctcgg tcgtaatgat ttctataatg acgaaaaaaa
     3841 aaaaattgga aagaaaaagc ttcatggcct ttataaaaag gaactatcca atacctcgcc
     3901 agaaccaagt aacagtattt tacggggcac aaatcaagaa caataagaCA GGACTGTAAA
     3961 GATGGACGCA TAGCctcgag ggggggcccg gtacCCAGCT TTTGTTCCCT TTAGTGAGGG
     4021 TTAATTCCGA GCTTGGCGTA ATCATGGTCA TAGCTGTTTC CTGTGTGAAA TTGTTATCCG
     4081 CTCACAATTC CACACAACAT ACGAGCCGGA AGCATAAAGT GTAAAGCCTG GGGTGCCTAA
     4141 TGAGTGAGGT AACTCACATT AATTGCGTTG CGCTCACTGC CCGCTTTCCA GTCGGGAAAC
     4201 CTGTCGTGCC AGCTGCATTA ATGAATCGGC CAACGCGCGG GGAGAGGCGG TTTGCGTATT
     4261 GGGCGCTCTT CCGCTTCCTC GCTCACTGAC TCGCTGCGCT CGGTCGTTCG GCTGCGGCGA
     4321 GCGGTATCAG CTCACTCAAA GGCGGTAATA CGGTTATCCA CAGAATCAGG GGATAACGCA
     4381 GGAAAGAACA TGTGAGCAAA AGGCCAGCAA AAGGCCAGGA ACCGTAAAAA GGCCGCGTTG
     4441 CTGGCGTTTT TCCATAGGCT CCGCCCCCCT GACGAGCATC ACAAAAATCG ACGCTCAAGT
     4501 CAGAGGTGGC GAAACCCGAC AGGACTATAA AGATACCAGG CGTTTCCCCC TGGAAGCTCC
     4561 CTCGTGCGCT CTCCTGTTCC GACCCTGCCG CTTACCGGAT ACCTGTCCGC CTTTCTCCCT
     4621 TCGGGAAGCG TGGCGCTTTC TCATAGCTCA CGCTGTAGGT ATCTCAGTTC GGTGTAGGTC
     4681 GTTCGCTCCA AGCTGGGCTG TGTGCACGAA CCCCCCGTTC AGCCCGACCG CTGCGCCTTA
     4741 TCCGGTAACT ATCGTCTTGA GTCCAACCCG GTAAGACACG ACTTATCGCC ACTGGCAGCA
     4801 GCCACTGGTA ACAGGATTAG CAGAGCGAGG TATGTAGGCG GTGCTACAGA GTTCTTGAAG
     4861 TGGTGGCCTA ACTACGGCTA CACTAGAAGG ACAGTATTTG GTATCTGCGC TCTGCTGAAG
     4921 CCAGTTACCT TCGGAAAAAG AGTTGGTAGC TCTTGATCCG GCAAACAAAC CACCGCTGGT
     4981 AGCGGTGGTT TTTTTGTTTG CAAGCAGCAG ATTACGCGCA GAAAAAAAGG ATCTCAAGAA
     5041 GATCCTTTGA TCTTTTCTAC GGGGTCTGAC GCTCAGTGGA ACGAAAACTC ACGTTAAGGG
     5101 ATTTTGGTCA TGAGATTATC AAAAAGGATC TTCACCTAGA TCCTTTTAAA TTAAAAATGA
     5161 AGTTTTAAAT CAATCTAAAG TATATATGAG TAAACTTGGT CTGACAGTTA CCAATGCTTA
     5221 ATCAGTGAGG CACCTATCTC AGCGATCTGT CTATTTCGTT CATCCATAGT TGCCTGACTC
     5281 CCCGTCGTGT AGATAACTAC GATACGGGAG GGCTTACCAT CTGGCCCCAG TGCTGCAATG
     5341 ATACCGCGAG ACCCACGCTC ACCGGCTCCA GATTTATCAG CAATAAACCA GCCAGCCGGA
     5401 AGGGCCGAGC GCAGAAGTGG TCCTGCAACT TTATCCGCCT CCATCCAGTC TATTAATTGT
     5461 TGCCGGGAAG CTAGAGTAAG TAGTTCGCCA GTTAATAGTT TGCGCAACGT TGTTGCCATT
     5521 GCTACAGGCA TCGTGGTGTC ACGCTCGTCG TTTGGTATGG CTTCATTCAG CTCCGGTTCC
     5581 CAACGATCAA GGCGAGTTAC ATGATCCCCC ATGTTGTGCA AAAAAGCGGT TAGCTCCTTC
     5641 GGTCCTCCGA TCGTTGTCAG AAGTAAGTTG GCCGCAGTGT TATCACTCAT GGTTATGGCA
     5701 GCACTGCATA ATTCTCTTAC TGTCATGCCA TCCGTAAGAT GCTTTTCTGT GACTGGTGAG
     5761 TACTCAACCA AGTCATTCTG AGAATAGTGT ATGCGGCGAC CGAGTTGCTC TTGCCCGGCG
     5821 TCAATACGGG ATAATACCGC GCCACATAGC AGAACTTTAA AAGTGCTCAT CATTGGAAAA
     5881 CGTTCTTCGG GGCGAAAACT CTCAAGGATC TTACCGCTGT TGAGATCCAG TTCGATGTAA
     5941 CCCACTCGTG CACCCAACTG ATCTTCAGCA TCTTTTACTT TCACCAGCGT TTCTGGGTGA
     6001 GCAAAAACAG GAAGGCAAAA TGCCGCAAAA AAGGGAATAA GGGCGACACG GAAATGTTGA
     6061 ATACTCATAC TCTTCCTTTT TCAATATTAT TGAAGCATTT ATCAGGGTTA TTGTCTCATG
     6121 AGCGGATACA TATTTGAATG TATTTAGAAA AATAAACAAA TAGGGGTTCC GCGCACATTT
     6181 CCCCGAAAAG TGCCACCTGA CGTCTAAGAA ACCATTATTA TCATGACATT AACCTATAAA
     6241 AATAGGCGTA TCACGAGGCC CTTTCGTC   
//
