LOCUS       Exported                6423 bp ds-DNA     circular SYN 07-SEP-2017
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  recombinant plasmid
REFERENCE   1  (bases 1 to 6423)
  AUTHORS   Bikard Lab
  TITLE     Direct Submission
  JOURNAL   Exported Aug 28, 2018 from SnapGene 4.2.4
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..6423
                     /organism="recombinant plasmid"
                     /mol_type="other DNA"
     misc_feature    1..5
                     /label=BioBrick suffix
                     /note="universal suffix for all parts"
     primer_bind     complement(20..36)
                     /label=LC256
                     /note="GFPmut2_R2"
     primer_bind     20..36
                     /label=LC523
                     /note="Ter_F"
     primer_bind     complement(22..41)
                     /label=LC125
                     /note="pITseq_R"
     terminator      30..92
                     /label=rrn BT2 terminator
     terminator      complement(107..199)
                     /label=tonB terminator
     primer_bind     140..150
                     /label=AB37 (2)
                     /note="ykoV_hom_Down_F"
     primer_bind     140..150
                     /label=AB37
                     /note="ykoV_hom_Down_F"
     primer_bind     complement(150..176)
                     /label=LC714
                     /note="pLC68_NGS_P1_R"
     primer_bind     complement(153..163)
                     /label=AB37 (2)
                     /note="ykoV_hom_Down_F"
     primer_bind     complement(153..163)
                     /label=AB37
                     /note="ykoV_hom_Down_F"
     primer_bind     complement(200..216)
                     /label=ES83
                     /note="compl"
     primer_bind     complement(200..214)
                     /label=ES59
                     /note="dCas9_primer3"
     primer_bind     complement(201..214)
                     /label=ES6
                     /note="pOSI-KH pTet_RBS2_dcas9_primer1"
     primer_bind     complement(204..226)
                     /label=BG75
                     /note="terminator_R"
     primer_bind     complement(205..226)
                     /label=BG68
                     /note="mcherry_psgRNAR"
     primer_bind     complement(216..239)
                     /label=LC524
                     /note="Ter_R"
     primer_bind     225..250
                     /label=LC536
                     /note="pIT_F1"
     misc_recomb     244..277
                     /label=FRT site sequence
     primer_bind     complement(274..292)
                     /label=ES7
                     /note="pIT5CO_Primer10"
     primer_bind     281..299
                     /label=LC669
                     /note="Clts_F"
     CDS             complement(284..997)
                     /label=C1 cds
     CDS             complement(284..997)
                     /label=lambda CI-ts (helper plasmid)
     primer_bind     284..301
                     /label=888 new kpn and avrII CIts side
     primer_bind     284..301
                     /label=402 LamCI3'EcoRI
     primer_bind     287..306
                     /label=900 lamCI 186CII chimeric rev
     primer_bind     304..325
                     /label=LC340
                     /note="CIts_F"
     primer_bind     complement(339..361)
                     /label=LC341
                     /note="Cits_R"
     primer_bind     396..413
                     /label=705 lamcI BsiWI
     primer_bind     complement(401..418)
                     /label=449 Lam CI L
     primer_bind     604..619
                     /label=717 lam cI Bsgl -2
     primer_bind     616..629
                     /label=SM13
                     /note="spa_spacer_m8_F"
     primer_bind     617..629
                     /label=SM27
                     /note="spa_spacer_m3_F"
     primer_bind     617..629
                     /label=SM25
                     /note="spa_spacer_m1_F"
     primer_bind     617..629
                     /label=SM23
                     /note="spa_spacer_m2_N_F"
     primer_bind     617..629
                     /label=SM11
                     /note="spa_spacer_m6_F"
     primer_bind     617..629
                     /label=SM9
                     /note="spa_spacer_m4_F"
     primer_bind     617..629
                     /label=SM7
                     /note="spa_spacer_m2_F"
     primer_bind     618..629
                     /label=B19
                     /note="K12sp2-R"
     primer_bind     complement(698..718)
                     /label=311 Lam CI C-dom
     primer_bind     779..812
                     /label=442 cI857 TOP
     primer_bind     complement(779..812)
                     /label=443 CI857 BOT
     primer_bind     complement(799..816)
                     /label=444 cI857 probe
     primer_bind     complement(802..821)
                     /label=718 lamm cI BsiWI -2
     primer_bind     936..954
                     /label=815 PstI lambda pRM
     primer_bind     937..954
                     /label=812 XbaI-pRM-pR
     primer_bind     939..958
                     /label=478 LCI fuse Bam
     primer_bind     961..981
                     /label=305 pRM +37
     primer_bind     complement(978..998)
                     /label=323 lambda Nde
     primer_bind     1007..1034
                     /label=623 37947_pRM-_XhoI
     primer_bind     1007..1034
                     /label=624 37947_pRM-_XbaI
     primer_bind     1008..1037
                     /label=800 JP_AatII_upLamPr
     primer_bind     1008..1034
                     /label=646 lambdaOR top-XbaI
     protein_bind    complement(1008..1024)
                     /label=Lambda OR3 operator
     primer_bind     1009..1021
                     /label=LC456
                     /note="LacZ11_F1"
     primer_bind     1011..1036
                     /label=458 37954_XhoI
     primer_bind     1011..1036
                     /label=459 37954_XbaI
     primer_bind     complement(1015..1034)
                     /label=342 oR3 alone
     primer_bind     1016..1041
                     /label=842 lam pR upstream
     protein_bind    complement(1031..1047)
                     /label=Lambda OR2 operator
     primer_bind     complement(1042..1062)
                     /label=304 pRM -65
     protein_bind    complement(1055..1071)
                     /label=Lambda OR1 operator
     primer_bind     complement(1060..1080)
                     /label=801 JP_HindIII_downLamPr
     primer_bind     complement(1068..1091)
                     /label=843 lam pR downstream
     primer_bind     complement(1068..1087)
                     /label=749 pR 3 prime
     primer_bind     complement(1078..1099)
                     /label=909 rev PC3 SbfI DraIII
     primer_bind     complement(1078..1099)
                     /label=916 rev p5 MluI DraIII
     primer_bind     complement(1079..1099)
                     /label=LC674
                     /note="Clts_R"
     primer_bind     1106..1125
                     /label=919 for Mlu Lam INT
     primer_bind     1167..1184
                     /label=ES36
                     /note="lambda/pKD4//int-primer4-verif"
     CDS             1239..2309
                     /label=lambda integrase cds
     CDS             1239..2309
                     /label=lambda integrase CDS
     primer_bind     complement(1302..1317)
                     /label=ES55
                     /note="lambda/pKD4//xls-primer3-verif"
     primer_bind     2061..2080
                     /label=156 lambda int
     primer_bind     2168..2177
                     /label=LC783
                     /note="gmd_R"
     primer_bind     2269..2288
                     /label=462 lam attP 2
     primer_bind     2298..2314
                     /label=116 RUS
     primer_bind     2320..2344
                     /label=LC338
                     /note="pINT_F"
     misc_feature    2394..2430
                     /label=common sequence (between Int & repA101) in help
                     /label=common sequence (between Int & repA101) in helper p
     misc_feature    2394..2430
                     /label=common sequence (between Int & repA101) in help
                     /label=common sequence (between Int & repA101) in helper 
                     p(1)
     primer_bind     complement(2397..2416)
                     /label=LC927
                     /note="pLC110_BR1"
     primer_bind     complement(2404..2430)
                     /label=879 helper primer for pick out Int
     primer_bind     complement(2404..2430)
                     /label=883 helper primer for pick out Int
     primer_bind     complement(2405..2430)
                     /label=889 new Xhol and DraIII INT side
     primer_bind     2423..2444
                     /label=LC931
                     /note="pLC110_FF2"
     CDS             2536..3351
                     /label=KanR (less restriction enzyme site)
     primer_bind     complement(2898..2911)
                     /label=BG12
                     /note="fis_cont_R"
     primer_bind     3402..3422
                     /label=LC668
                     /note="pLC79_F"
     primer_bind     3402..3422
                     /label=LC583
                     /note="pLC64cos_F"
     primer_bind     3403..3422
                     /label=B248
                     /note="VR"
     primer_bind     3403..3422
                     /label=LC665
                     /note="pLC10_F2"
     primer_bind     3404..3422
                     /label=LC331
                     /note="p4BB_F"
     primer_bind     3407..3424
                     /label=LC217
                     /note="R6Kgamma_R"
     rep_origin      3442..3871
                     /label=R6Kgamma ori
     primer_bind     3824..3848
                     /label=V192
                     /note="R6K-up"
     primer_bind     3847..3864
                     /label=467 CRIM P2
     primer_bind     3847..3864
                     /label=LC81
                     /note="OSIP_P2"
     primer_bind     complement(3851..3871)
                     /label=LC219
                     /note="R6Kgamma_F"
     misc_recomb     3886..3919
                     /label=FRT site sequence(1)
     misc_recomb     3932..4318
                     /label=lambda att (Wanner)
     primer_bind     3998..4022
                     /label=LC338
                     /note="pINT_F"
     primer_bind     complement(4042..4058)
                     /label=ES35
                     /note="lambda/pKD4//int-primer3-verif"
     primer_bind     complement(4245..4255)
                     /label=B278
                     /note="prtspc1-21f"
     primer_bind     complement(4245..4255)
                     /label=B277
                     /note="prtspc1-22f"
     primer_bind     complement(4245..4255)
                     /label=B276
                     /note="prtspc1-23f"
     terminator      complement(4325..4596)
                     /label=tL3 terminator
     primer_bind     4325..4345
                     /label=LC851
                     /note="ter_R1"
     primer_bind     4325..4345
                     /label=LC934
                     /note="pLC113_BF"
     primer_bind     4325..4345
                     /label=LC857
                     /note="Cas9Ter_R2"
     primer_bind     4325..4345
                     /label=LC727
                     /note="pLC93_BB_F"
     primer_bind     4325..4345
                     /label=LC725
                     /note="pLC92_BB_F"
     primer_bind     4325..4345
                     /label=LC1074
                     /note="pLC134_R1"
     primer_bind     4325..4345
                     /label=LC1086
                     /note="pLC141_R"
     primer_bind     4357..4373
                     /label=ES43
                     /note="lambda/pKD4//orf60a-primer3-verif"
     primer_bind     4361..4384
                     /label=LC379
                     /note="pCasRev"
     primer_bind     4361..4384
                     /label=LC1034
                     /note="pLC115_F1_R"
     primer_bind     complement(4376..4394)
                     /label=LC236
                     /note="Orits_F1"
     primer_bind     complement(4376..4394)
                     /label=LC232
                     /note="orits_F"
     primer_bind     complement(4385..4402)
                     /label=468 CRIM P3
     primer_bind     complement(4385..4402)
                     /label=LC82
                     /note="OSIP_P3"
     primer_bind     4461..4472
                     /label=LC394
                     /note="PA-LigD_F"
     primer_bind     complement(4556..4572)
                     /label=ES52
                     /note="lambda/pKD4//orf63-primer4-verif"
     primer_bind     complement(4569..4590)
                     /label=LC359
                     /note="pCasBB_F"
     primer_bind     complement(4569..4589)
                     /label=LC342
                     /note="pCasBB_F"
     primer_bind     complement(4672..4692)
                     /label=LC887
                     /note="pLC104_R"
     primer_bind     complement(4672..4691)
                     /label=LC932
                     /note="pLC110_FR2"
     primer_bind     complement(4672..4691)
                     /label=LC363
                     /note="pUC19BB_R"
     primer_bind     4731..4751
                     /label=oGR351
                     /note="Fw_seq_pEX-K4"
     primer_bind     4764..4773
                     /label=LC110
                     /note="irhA_F"
     primer_bind     4808..4827
                     /label=LC1199
                     /note="DB_FOR"
     primer_bind     4847..4864
                     /label=oGR335
                     /note="vector_pRG13A_R"
     primer_bind     complement(4849..4867)
                     /label=LC1197
                     /note="pLC159B_REV"
     primer_bind     complement(4849..4867)
                     /label=LC193
                     /note="pZA31BB_R"
     primer_bind     complement(4849..4867)
                     /label=LC1046
                     /note="pLC118_F1"
     primer_bind     complement(4849..4867)
                     /label=LC534
                     /note="pIT_R1"
     primer_bind     complement(4850..4869)
                     /label=BG114
                     /note="psgRNAcos_qPCR"
     primer_bind     complement(4851..4869)
                     /label=LC508
                     /note="sgPB_R1"
     primer_bind     4852..4871
                     /label=LC1069
                     /note="pLC128_R1"
     primer_bind     4852..4871
                     /label=SB17
                     /note="SB17_Intetrate49"
     primer_bind     complement(4853..4871)
                     /label=ES75
                     /note="BB_primer5"
     primer_bind     4853..4870
                     /label=BG82
                     /note="psgRNAcos_strongprom"
     primer_bind     4853..4870
                     /label=BG80
                     /note="psgRNAcos_mediumprom"
     primer_bind     4853..4869
                     /label=LC287
                     /note="LacZ2mut1K_F"
     primer_bind     complement(4854..4873)
                     /label=BG64
                     /note="bla_pCas9_R"
     primer_bind     complement(4856..4879)
                     /label=LC915
                     /note="pLC108_BR"
     primer_bind     complement(4856..4877)
                     /label=LC34
                     /note="pCRISPR_BB_R"
     primer_bind     complement(4860..4878)
                     /label=BG61
                     /note="pZA31_R"
     primer_bind     complement(4860..4878)
                     /label=LC42
                     /note="ELZ2MutBB_R"
     primer_bind     4860..4874
                     /label=ES72
                     /note="primer6_fow_pES4"
     primer_bind     complement(4868..4885)
                     /label=B572
                     /note="(GFP)pKL55-R"
     primer_bind     complement(4872..4892)
                     /label=LC87
                     /note="OSIPlambda_P3"
     terminator      4899..5067
                     /label=rrnBT1 terminator
     primer_bind     4899..4920
                     /label=LC645
                     /note="pLC51_BB_F"
     primer_bind     4899..4914
                     /label=ES61
                     /note="pZA31luc_primer1"
     primer_bind     complement(4969..4991)
                     /label=SB18
                     /note="SB18_Intetrate49"
     primer_bind     4970..4991
                     /label=LC192
                     /note="pZA31BB_F"
     primer_bind     complement(4987..4997)
                     /label=LC191
                     /note="GFPmut2_R"
     primer_bind     5023..5032
                     /label=LC923
                     /note="pLC109_R2"
     terminator      5080..5248
                     /label=rrnBT1 terminator(1) terminator
                     /label=rrnBT1 terminator(1)
     primer_bind     5080..5101
                     /label=LC645
                     /note="pLC51_BB_F"
     primer_bind     5080..5095
                     /label=ES61
                     /note="pZA31luc_primer1"
     primer_bind     complement(5150..5172)
                     /label=SB18
                     /note="SB18_Intetrate49"
     primer_bind     5151..5172
                     /label=LC192
                     /note="pZA31BB_F"
     primer_bind     complement(5168..5178)
                     /label=LC191
                     /note="GFPmut2_R"
     primer_bind     5204..5213
                     /label=LC923
                     /note="pLC109_R2"
     terminator      5261..5429
                     /label=rrnBT1 terminator(2) terminator
                     /label=rrnBT1 terminator(2)
     primer_bind     5261..5282
                     /label=LC645
                     /note="pLC51_BB_F"
     primer_bind     5261..5276
                     /label=ES61
                     /note="pZA31luc_primer1"
     primer_bind     complement(5331..5353)
                     /label=SB18
                     /note="SB18_Intetrate49"
     primer_bind     5332..5353
                     /label=LC192
                     /note="pZA31BB_F"
     primer_bind     complement(5349..5359)
                     /label=LC191
                     /note="GFPmut2_R"
     primer_bind     5385..5394
                     /label=LC923
                     /note="pLC109_R2"
     terminator      5442..5610
                     /label=rrnBT1 terminator(3) terminator
                     /label=rrnBT1 terminator(3)
     primer_bind     5442..5463
                     /label=LC645
                     /note="pLC51_BB_F"
     primer_bind     5442..5457
                     /label=ES61
                     /note="pZA31luc_primer1"
     primer_bind     complement(5512..5534)
                     /label=SB18
                     /note="SB18_Intetrate49"
     primer_bind     5513..5534
                     /label=LC192
                     /note="pZA31BB_F"
     primer_bind     complement(5530..5540)
                     /label=LC191
                     /note="GFPmut2_R"
     primer_bind     5566..5575
                     /label=LC923
                     /note="pLC109_R2"
     primer_bind     5582..5631
                     /label=V291
                     /note="p65-promoter-F"
     primer_bind     5596..5647
                     /label=V1
     primer_bind     5605..5623
                     /label=LC124
                     /note="pITseq_F"
     primer_bind     5605..5623
                     /label=LC928
                     /note="pLC110_BF1"
     primer_bind     5626..5646
                     /label=B411
                     /note="spB3-R"
     primer_bind     complement(5627..5658)
                     /label=5'probe GFP
                     /note="from pDB127 pZS2(pam)prom-GFPmut2"
     primer_bind     5629..5658
                     /label=B413
                     /note="spB4-R"
     primer_bind     5629..5648
                     /label=BG67
                     /note="mcherry_psgRNAcosF"
     primer_bind     complement(5635..5664)
                     /label=B393
                     /note="spT3-R"
     primer_bind     5642..5686
                     /label=B369a
                     /note="pamPromoter-F"
     primer_bind     5642..5686
                     /label=B369
     primer_bind     complement(5646..5690)
                     /label=B370a
                     /note="pamPromoter-R"
     primer_bind     complement(5646..5690)
                     /label=B370
     primer_bind     complement(5648..5677)
                     /label=B395
                     /note="spT4-R"
     misc_feature    5650..5654
                     /label=-35
     misc_feature    complement(5651..5680)
                     /label=Antoine pro_R1
     primer_bind     5651..5680
                     /label=V210
                     /note="DB127-0F-R"
     primer_bind     5651..5680
                     /label=B415
                     /note="spB5-R"
     misc_feature    complement(5651..5670)
                     /label=mcherry promoter sgRNA
     primer_bind     5651..5670
                     /label=V220
                     /note="p35-R"
     primer_bind     complement(5651..5670)
                     /label=V219
                     /note="p35-F"
     primer_bind     complement(5654..5683)
                     /label=B490
                     /note="spT5m12-R"
     primer_bind     complement(5654..5683)
                     /label=B488
                     /note="spT5m10-R"
     primer_bind     complement(5654..5683)
                     /label=B486
                     /note="spT5m8-R"
     primer_bind     complement(5654..5683)
                     /label=B484
                     /note="spT5m6-R"
     primer_bind     complement(5654..5683)
                     /label=B482
                     /note="spT5m4-R"
     primer_bind     complement(5654..5683)
                     /label=B480
                     /note="spT5m2-R"
     primer_bind     complement(5654..5683)
                     /label=B397
                     /note="spT5-R"
     primer_bind     5663..5692
                     /label=B417
                     /note="spB6-R"
     primer_bind     complement(5670..5699)
                     /label=B399
                     /note="spT6-R"
     primer_bind     5672..5706
                     /label=V327
                     /note="decoy2-20-F"
     misc_feature    5673..5678
                     /label=-10
     primer_bind     5674..5703
                     /label=B419
                     /note="spB7-R"
     primer_bind     complement(5684..5721)
                     /label=V36
                     /note="(mRFP1)-promoter-R"
     primer_bind     complement(5685..5736)
                     /label=V40
                     /note="(mCherryE)-pDB127promoter-R"
     primer_bind     complement(5685..5730)
                     /label=V5
                     /note="(mCherryH)-pDB127promoter-R"
     primer_bind     5686..5715
                     /label=B421
                     /note="spB8-R"
     primer_bind     complement(5691..5715)
                     /label=B401
                     /note="spT7-R"
     primer_bind     complement(5691..5715)
                     /label=V2
                     /note="(sfGFP)-pDB127promoter-R"
     RBS             5696..5707
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
     primer_bind     5701..5736
                     /label=V38
                     /note="(promoter)-mCherryE-F"
     primer_bind     5701..5734
                     /label=V6
                     /note="(promoter)-mCherryH-F"
     primer_bind     5701..5733
                     /label=V37
                     /note="(promoter)-mStrawberry-F"
     primer_bind     5712..5732
                     /label=LC249
                     /note="mcherry_F1"
     primer_bind     5712..5731
                     /label=V69
                     /note="prefix-mCherry-F"
     CDS             5713..6423
                     /label=mCherry E
     primer_bind     5713..5743
                     /label=V113_pAV9_mCherry_F
     primer_bind     5713..5732
                     /label=LC506
                     /note="sulAmcherryF"
     primer_bind     5713..5731
                     /label=V77_pLac-mch-F
     primer_bind     5714..5733
                     /label=V235
                     /note="p43-mCh-F"
     primer_bind     5721..5735
                     /label=B214
                     /note="5'srtA-mCherry-F"
     primer_bind     5721..5732
                     /label=LC364
                     /note="mcherry_F2"
     primer_bind     complement(5814..5865)
                     /label=V188
                     /note="R55-R"
     primer_bind     5835..5864
                     /label=V147
                     /note="sgRNA-mCherry-0-mismatch-R"
     primer_bind     complement(5835..5864)
                     /label=V146
                     /note="sgRNA-mCherry-0-mismatch-F"
     primer_bind     5835..5864
                     /label=V99
                     /note="M9_first_R"
     primer_bind     5835..5864
                     /label=V89
                     /note="M0_first_R"
     misc_feature    complement(5835..5854)
                     /label=sgRNA mcherry ORF
     primer_bind     5835..5854
                     /label=V226
                     /note="p38-R"
     primer_bind     complement(5835..5854)
                     /label=V225
                     /note="p38-F"
     primer_bind     5835..5854
                     /label=V91
                     /note="Probe_M0"
     primer_bind     complement(6025..6046)
                     /label=V123
                     /note="mCherry-upstream"
     primer_bind     complement(6084..6107)
                     /label=B243
                     /note="mRFP-R"
     primer_bind     6201..6220
                     /label=V85
                     /note="Screen_mCherry_F"
     primer_bind     6269..6285
                     /label=oGR386
                     /note="pRG72_mcherry_F"
     primer_bind     complement(6385..6420)
                     /label=V114_pAV9_mCherry_R
     primer_bind     complement(join(6397..6423,1..3))
                     /label=V139
                     /note="pAV12-R"
     primer_bind     complement(6398..6420)
                     /label=V236
                     /note="p43-mCh-R"
     primer_bind     complement(join(6399..6423,1..36))
                     /label=LC246
                     /note="GFP_R"
     primer_bind     complement(6399..6423)
                     /label=oGR374
                     /note="mcherry_FRAG_R"
     primer_bind     complement(join(6400..6423,1..36))
                     /label=LC255
                     /note="GFPmut2_R1"
     primer_bind     complement(join(6400..6423,1..32))
                     /label=V39
                     /note="(pOSIP)-mCherryE-R"
     primer_bind     complement(join(6400..6423,1..20))
                     /label=V78_osip-mch-R
     primer_bind     complement(join(6400..6423,1..2))
                     /label=oGR14
                     /note="Fse_SF_GFP_R"
     primer_bind     complement(join(6400..6423,1..2))
                     /label=oGR373
                     /note="GFP_FRAG_F"
     primer_bind     complement(join(6400..6423,1))
                     /label=V152
                     /note="pAV17-B-R"
     primer_bind     complement(6400..6423)
                     /label=B368
                     /note="BamHI-GFPmut2-R"
     primer_bind     complement(6400..6423)
                     /label=B574
                     /note="(pKL55)GFP-R"
     primer_bind     complement(6400..6420)
                     /label=BG84
                     /note="sfGFP_fastag"
     primer_bind     complement(6400..6420)
                     /label=BG78
                     /note="sfGFP_mediumtag"
     primer_bind     complement(join(6401..6423,1))
                     /label=B215
                     /note="mCherry-3'srtA-R"
     primer_bind     complement(6401..6423)
                     /label=B659
                     /note="BglII-GFP-R"
     primer_bind     complement(6401..6420)
                     /label=V70
                     /note="suffix-mCherry-R"
     primer_bind     complement(join(6402..6423,1..36))
                     /label=LC366
                     /note="mcherry_R3"
     primer_bind     complement(6403..6419)
                     /label=LC247
                     /note="mcherry_R1"
     primer_bind     join(6421..6423,1..19)
                     /label=BG77
                     /note="terminator_fast"
     primer_bind     join(6421..6423,1..19)
                     /label=BG76
                     /note="terminator_medium"
ORIGIN
        1 tgcaggcatg cctcgagatg catggcgcct aacctaaact gacaggcatc aaattaagca
       61 gaaggccatc ctgacggatg gcctttttgc gtttcgaaca attgaaaaaa cctcgcgcct
      121 tacctgttga gtaatagtca aaagcctccg gtcggaggct tttgactttc tgcttactga
      181 atttcggtgg tgccgttaat taaccggtgg gccctcatga taataatggt ttcttagacg
      241 tccgaagttc ctattctcta gaaagtatag gaacttccct aggtcagcca aacgtctctt
      301 caggccactg actagcgata actttcccca caacggaaca actctcattg catgggatca
      361 ttgggtactg tgggtttagt ggttgtaaaa acacctgacc gctatccctg atcagtttct
      421 tgaaggtaaa ctcatcaccc ccaagtctgg ctatgcagaa atcacctggc tcaacagcct
      481 gctcagggtc aacgagaatt aacattccgt caggaaagct tggcttggag cctgttggtg
      541 cggtcatgga attaccttca acctcaagcc agaatgcaga atcactggct tttttggttg
      601 tgcttaccca tctctccgca tcacctttgg taaaggttct aagcttaggt gagaacatcc
      661 ctgcctgaac atgagaaaaa acagggtact catactcact tctaagtgac ggctgcatac
      721 taaccgcttc atacatctcg tagatttctc tggcgattga agggctaaat tcttcaacgc
      781 taactttgag aatttttgta agcaatgcgg cgttataagc atttaatgca ttgatgccat
      841 taaataaagc accaacgcct gactgcccca tccccatctt gtctgcgaca gattcctggg
      901 ataagccaag ttcatttttc tttttttcat aaattgcttt aaggcgacgt gcgtcctcaa
      961 gctgctcttg tgttaatggt ttcttttttg tgctcatacg ttaaatctat caccgcaagg
     1021 gataaatatc taacaccgtg cgtgttgact attttacctc tggcggtgat aatggttgca
     1081 tgtactaagg aggttgtata cgcgtcgtcg atgggttcgg gaatgcagga tattcccacc
     1141 tccggttaag gatggaagag agtatctgtt ccacgaatca gcggtaaagg ttgacttaaa
     1201 tcgaccagta acaggtggcc ttttgaagag gatcagaaat gggaagaagg cgaagtcatg
     1261 agcgccggga tttaccccct aacctttata taagaaacaa tggatattac tgctacaggg
     1321 acccaaggac gggtaaagag tttggattag gcagagacag gcgaatcgca atcactgaag
     1381 ctatacaggc caacattgag ttattttcag gacacaaaca caagcctctg acagcgagaa
     1441 tcaacagtga taattccgtt acgttacatt catggcttga tcgctacgaa aaaatcctgg
     1501 ccagcagagg aatcaagcag aagacactca taaattacat gagcaaaatt aaagcaataa
     1561 ggaggggtct gcctgatgct ccacttgaag acatcaccac aaaagaaatt gcggcaatgc
     1621 tcaatggata catagacgag ggcaaggcgg cgtcagccaa gttaatcaga tcaacactga
     1681 gcgatgcatt ccgagaggca atagctgaag gccatataac aacaaaccat gtcgctgcca
     1741 ctcgcgcagc aaaatcagag gtaaggagat caagacttac ggctgacgaa tacctgaaaa
     1801 tttatcaagc agcagaatca tcaccatgtt ggctcagact tgcaatggaa ctggctgttg
     1861 ttaccgggca acgagttggt gatttatgcg aaatgaagtg gtctgatatc gtagatggat
     1921 atctttatgt cgagcaaagc aaaacaggcg taaaaattgc catcccaaca gcattgcata
     1981 ttgatgctct cggaatatca atgaaggaaa cacttgataa atgcaaagag attcttggcg
     2041 gagaaaccat aattgcatct actcgtcgcg aaccgctttc atccggcaca gtatcaaggt
     2101 attttatgcg cgcacgaaaa gcatcaggtc tttccttcga aggggatccg cctacctttc
     2161 acgagttgcg cagtttgtct gcaagactct atgagaagca gataagcgat aagtttgctc
     2221 aacatcttct cgggcataag tcggacacca tggcatcaca gtatcgtgat gacagaggca
     2281 gggagtggga caaaattgaa atcaaataat gattttattt tgactgatag tgacctgttc
     2341 gttgcaacaa attgataagc aatgccaagc ttggcactgg ctgatcagct agcccatggg
     2401 tatggacagt tttccctttg atatgtaacg cacgttgtgt ctcaaaatct ctgatgttac
     2461 attgcacaag ataaaaatat atcatcatga acaataaaac tgtctgctta cataaacagt
     2521 aatacaaggg gtgttatgag ccatattcaa cgggaaacgt cttgctcccg tccgcgctta
     2581 aactccaaca tggacgctga tttatatggg tataaatggg ctcgcgataa tgtcgggcaa
     2641 tcaggtgcga caatctatcg cttgtatggg aagcccgatg cgccagagtt gtttctgaaa
     2701 catggcaaag gtagcgttgc caatgatgtt acagatgaga tggtccgtct caactggctg
     2761 acggagttta tgcctctccc gaccatcaag cattttatcc gtactcctga tgatgcgtgg
     2821 ttactcacca ccgcgattcc tgggaaaaca gccttccagg tattagaaga atatcctgat
     2881 tcaggtgaaa atattgttga tgcgctggcc gtgttcctgc gccggttaca ttcgattcct
     2941 gtttgtaatt gtccttttaa cagcgatcgt gtatttcgtc ttgctcaggc gcaatcacgc
     3001 atgaataacg gtttggttga tgcgagtgat tttgatgacg agcgtaatgg ctggcctgtt
     3061 gaacaagtct ggaaagaaat gcacaagctc ttgccattct caccggattc agtcgtcact
     3121 catggtgatt tctcacttga taaccttatt tttgacgagg ggaaattaat aggttgtatt
     3181 gatgttggac gggtcggaat cgcagaccgt taccaggacc ttgccattct ttggaactgc
     3241 ctcggtgagt tttctccttc attacagaaa cggctttttc aaaaatatgg tattgataat
     3301 cctgatatga ataaattgca gtttcatttg atgctcgatg agtttttcta ataatactag
     3361 cagaaatcat ccttagcgaa agctaaggat tttttttatc tgattaccgc ctttgagtga
     3421 gcgtcgacct agtgcggccg caagatccgg ccacgatgcg tccggcgtag aggatctgaa
     3481 gatcagcagt tcaacctgtt gatagtacgt actaagctct catgtttcac gtactaagct
     3541 ctcatgttta acgtactaag ctctcatgtt taacgaacta aaccctcatg gctaacgtac
     3601 taagctctca tggctaacgt actaagctct catgtttcac gtactaagct ctcatgtttg
     3661 aacaataaaa ttaatataaa tcagcaactt aaatagcctc taaggtttta agttttataa
     3721 gaaaaaaaag aatatataag gcttttaaag cttttaaggt ttaacggttg tggacaacaa
     3781 gccagggatg taacgcactg agaagccctt agagcctctc aaagcaattt tgagtgacac
     3841 aggaacactt aacggctgac atgggaatta ggaagttcct attccgaagt tcctattctc
     3901 tagaaagtat aggaacttcc atatgccatg gcatcacagt atcgtgatga cagaggcagg
     3961 gagtgggaca aaattgaaat caaataatga ttttattttg actgatagtg acctgttcgt
     4021 tgcaacaaat tgataagcaa tgctttttta taatgccaac ttagtataaa aaagctgaac
     4081 gagaaacgta aaatgatata aatatcaata tattaaatta gattttgcat aaaaaacaga
     4141 ctacataata ctgtaaaaca caacatatat gcagtcacta tgaatcaact acttagatgg
     4201 tattagtgac ctgtaacaga gcattagcgc aaggtgattt ttgtcttctt gcgctaattt
     4261 tttgtcatca aacctgtcgc actccagaga agcacaaagc ctcgcaatcc agtgcaaagc
     4321 tagcttcttc gtctgtttct actggtattg gcacaaacct gattccaatt tgagcaaggc
     4381 tatgtgccat ctcgatactc gttcttaact caacagaaga tgctttgtgc atacagcccc
     4441 tcgtttatta tttatctcct cagccagccg ctgtgctttc agtggatttc ggataacaga
     4501 aaggccggga aatacccagc ctcgctttgt aacggagtag agacgaaagt gattgcgcct
     4561 acccggatat tatcgtgagg atgcgtcatc gccattaatt cactgatcag tgatagctgt
     4621 caaacatgag aattgatccg gctgcctcgc gcgtttcggt gatgacggtg aaaacctctg
     4681 acacatgcag ctcccggaga cggtcacagc ttgtctgtaa gcggatgccg ggagcagaca
     4741 agcccgtcag ggcgcgtcag cgggtgttgg cgggtgtcgg ggcgcagcca tgacccagtc
     4801 acgtagcgat agcggagtgt atgctgcaca tgacattaac ctataaaaat aggcgtatca
     4861 cgaggccctt tcgtcttcaa gaattaattc ccaattccag gcatcaaata aaacgaaagg
     4921 ctcagtcgaa agactgggcc tttcgtttta tctgttgttt gtcggtgaac gctctcctga
     4981 gtaggacaaa tccgccggga gcggatttga acgttgcgaa gcaacggccc ggagggtggc
     5041 gggcaggacg cccgccataa actgccagga attaattcca ggcatcaaat aaaacgaaag
     5101 gctcagtcga aagactgggc ctttcgtttt atctgttgtt tgtcggtgaa cgctctcctg
     5161 agtaggacaa atccgccggg agcggatttg aacgttgcga agcaacggcc cggagggtgg
     5221 cgggcaggac gcccgccata aactgccagg aattaattcc aggcatcaaa taaaacgaaa
     5281 ggctcagtcg aaagactggg cctttcgttt tatctgttgt ttgtcggtga acgctctcct
     5341 gagtaggaca aatccgccgg gagcggattt gaacgttgcg aagcaacggc ccggagggtg
     5401 gcgggcagga cgcccgccat aaactgccag gaattaattc caggcatcaa ataaaacgaa
     5461 aggctcagtc gaaagactgg gcctttcgtt ttatctgttg tttgtcggtg aacgctctcc
     5521 tgagtaggac aaatccgccg ggagcggatt tgaacgttgc gaagcaacgg cccggagggt
     5581 ggcgggcagg acgcccgcca taaactgcca ggaattgggg atcggcctat gaactgtcga
     5641 ctcgaggcct tgacaggtac ctcatggata cctataatgg ttccggaatt cctttaaaga
     5701 ggagaaatct agatggtttc caagggcgag gaggataaca tggctatcat taaagagttc
     5761 atgcgcttca aagttcacat ggagggttct gttaacggtc acgagttcga gatcgaaggc
     5821 gaaggcgagg gccgtccgta tgaaggcacc cagaccgcca aactgaaagt gactaaaggc
     5881 ggcccgctgc cttttgcgtg ggacatcctg agcccgcaat ttatgtacgg ttctaaagct
     5941 tatgttaaac acccagcgga tatcccggac tatctgaagc tgtcttttcc ggaaggtttc
     6001 aagtgggaac gcgtaatgaa ttttgaagat ggtggtgtcg tgaccgtcac tcaggactcc
     6061 tccctgcagg atggcgagtt catctataaa gttaaactgc gtggtactaa ttttccatct
     6121 gatggcccgg tgatgcagaa gaagacgatg ggttgggagg cgtctagcga acgcatgtat
     6181 ccggaagatg gtgcgctgaa aggcgaaatt aaacagcgcc tgaaactgaa agatggcggc
     6241 cattatgacg ctgaagtgaa aaccacgtac aaagccaaga aacctgtgca gctgcctggc
     6301 gcgtacaatg tgaatattaa actggacatc acctctcata atgaagatta tacgatcgta
     6361 gagcaatatg agcgcgcgga gggtcgtcat tctaccggtg gcatggatga gctgtacaaa
     6421 taa
//
