LOCUS       R208_pET32-MCM2:       10930 bp    DNA     circular SYN 28-Aug-2018
DEFINITION  -
ACCESSION   -
KEYWORDS    -
SOURCE      -
FEATURES             Location/Qualifiers
     promoter        529..557
                     /label=pAmp
                     /note="AmpR_promoter"
     CDS             599..1459
                     /codon_start=1
                     /db_xref="GI:455370"
                     /gene="bla"
                     /label=AmpR
                     /note="E-286"
                     /product="beta-lactamase"
                     /protein_id="AAB59737.1"
                     /transl_table=11
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      1630..2218
                     /label=pMB1 ori
                     /note="pMB1 origin of replication (clockwise) (RNAII -35
                     to RNA/DNA switch point)"
     CDS             complement(3648..4739)
                     /note="lacI"
     promoter        5121..5142
                     /note="T7 promoter"
     misc_binding    5140..5164
                     /bound_moiety=""
                     /label=lacO
     CDS             5209..7842
                     /note="Thioredoxin-His6-hMCM7 (877 aa)"
                     /translation="MSDKIIHLTDDSFDTDVLKADGAILVDFWAEWCGPCKMIAPILDE
                     IADEYQGKLTVAKLNIDQNPGTAPKYGIRGIPTLLLFKNGEVAATKVGALSKGQLKEFL
                     DANLAGSGSGHMHHHHHHSSGLVPRGSGMKETAAAKFERQHMDSPDLGTDDDDKMALKD
                     YALEKEKVKKFLQEFYQDDELGKKQFKYGNQLVRLAHREQVALYVDLDDVAEDDPELVD
                     SICENARRYAKLFADAVQELLPQYKEREVVNKDVLDVYIEHRLMMEQRSRDPGMVRSPQ
                     NQYPAELMRRFELYFQGPSSNKPRVIREVRADSVGKLVTVRGIVTRVSEVKPKMVVATY
                     TCDQCGAETYQPIQSPTFMPLIMCPSQECQTNRSGGRLYLQTRGSRFIKFQEMKMQEHS
                     DQVPVGNIPRSITVLVEGENTRIAQPGDHVSVTGIFLPILRTGFRQVVQGLLSETYLEA
                     HRIVKMNKSEDDESGAGELTREELRQIAEEDFYEKLAASIAPEIYGHEDVKKALLLLLV
                     GGVDQSPRGMKIRGNINICLMGDPGVAKSQLLSYIDRLAPRSQYTTGRGSSGVGLTAAV
                     LRDSVSGELTLEGGALVLADQGVCCIDEFDKMAEADRTAIHEVMEQQTISIAKAGILTT
                     LNARCSILAAANPAYGRYNPRRSLEQNIQLPAALLSRFDLLWLIQDRPDRDNDLRLAQH
                     ITYVHQHSRQPPSQFEPLDMKLMRRYIAMCREKQPMVPESLADYITAAYVEMRREAWAS
                     KDATYTSARTLLAILRLSTALARLRMVDVVEKEDVNEAIRLMEMSKDSLLGDKGQTART
                     QRPADVIFATVRELVSGGRSVRFSEAEQRCVSRGFTPAQFQAALDEYEELNVWQVNASR
                     TRITFV*"
     misc_feature    5557..5574
                     /note="6xHis"
     promoter        7876..7897
                     /note="T7 promoter"
     misc_binding    7895..7919
                     /bound_moiety=""
                     /label=lacO
     CDS             7963..10698
                     /note="hMCM2 (911 aa)"
                     /translation="MQAGPASMAESSESFTMASSPAQRRRGNDPLTSSPGRSSRRTDAL
                     TSSPGRDLPPFEDESEGLLGTEGPLEEEEDGEELIGDGMERDYRAIPELDAYEAEGLAL
                     DDEDVEELTASQREAAERAMRQRDREAGRGLGRMRRGLLYDSDEEDEERPARKRRQVER
                     ATEDGEEDEEMIESIENLEDLKGHSVREWVSMAGPRLEIHHRFKNFLRTHVDSHGHNVF
                     KERISDMCKENRESLVVNYEDLAAREHVLAYFLPEAPAELLQIFDEAALEVVLAMYPKY
                     DRITNHIHVRISHLPLVEELRSLRQLHLNQLIRTSGVVTSCTGVLPQLSMVKYNCNKCN
                     FVLGPFCQSQNQEVKPGSCPECQSAGPFEVNMEETIYQNYQRIRIQESPGKVAAGRLPR
                     SKDAILLADLVDSCKPGDEIELTGIYHNNYDGSLNTANGFPVFATVILANHVAKKDNKV
                     AVGELTDEDVKMITSLSKDQQIGEKIFASIAPSIYGHEDIKRGLALALFGGEPKNPGGK
                     HKVRGDINVLLCGDPGTAKSQFLKYIEKVSSRAIFTTGQGASAVGLTAYVQRHPVSREW
                     TLEAGALVLADRGVCLIDEFDKMNDQDRTSIHEAMEQQSISISKAGIVTSLQARCTVIA
                     AANPIGGRYDPSLTFSENVDLTEPIISRFDILCVVRDTVDPVQDEMLARFVVGSHVRHH
                     PSNKEEEGLANGSAAEPAMPNTYGVEPLPQEVLKKYIIYAKERVHPKLNQMDQDKVAKM
                     YSDLRKESMATGSIPITVRHIESMIRMAEAHARIHLRDYVIEDDVNMAIRVMLESFIDT
                     QKFSVMRSMRKTFARYLSFRRDNNELLLFILKQLVAEQVTYQRNRFGAQQDTIEVPEKD
                     LVDKARQINIHNLSAFYDSELFRMNKFSHDLKRKMILQQF*"
     terminator      10802..10930
                     /note="T7 Terminator"
BASE COUNT      2563 A      2946 C      3061 G      2360 T         0 OTHER
ORIGIN      ?
        1 TGGCGAATGG GACGCGCCCT GTAGCGGCGC ATTAAGCGCG GCGGGTGTGG TGGTTACGCG
       61 CAGCGTGACC GCTACACTTG CCAGCGCCCT AGCGCCCGCT CCTTTCGCTT TCTTCCCTTC
      121 CTTTCTCGCC ACGTTCGCCG GCTTTCCCCG TCAAGCTCTA AATCGGGGGC TCCCTTTAGG
      181 GTTCCGATTT AGTGCTTTAC GGCACCTCGA CCCCAAAAAA CTTGATTAGG GTGATGGTTC
      241 ACGTAGTGGG CCATCGCCCT GATAGACGGT TTTTCGCCCT TTGACGTTGG AGTCCACGTT
      301 CTTTAATAGT GGACTCTTGT TCCAAACTGG AACAACACTC AACCCTATCT CGGTCTATTC
      361 TTTTGATTTA TAAGGGATTT TGCCGATTTC GGCCTATTGG TTAAAAAATG AGCTGATTTA
      421 ACAAAAATTT AACGCGAATT TTAACAAAAT ATTAACGTTT ACAATTTCAG GTGGCACTTT
      481 TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA
      541 TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT
      601 GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT
      661 TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG
      721 AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA
      781 AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG
      841 TATTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT
      901 TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG
      961 CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG
     1021 AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA
     1081 TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC
     1141 TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC
     1201 CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC
     1261 GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG
     1321 CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC
     1381 GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC
     1441 ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT
     1501 AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC
     1561 CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA
     1621 AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC
     1681 ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT
     1741 AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG
     1801 CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC
     1861 AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT
     1921 ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA
     1981 GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT
     2041 TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG
     2101 CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA
     2161 CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA
     2221 CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT
     2281 CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA
     2341 TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA
     2401 GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG
     2461 TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT
     2521 CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT
     2581 GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT
     2641 GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT
     2701 CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT
     2761 TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG
     2821 TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA
     2881 TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC
     2941 GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA
     3001 AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA
     3061 GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG
     3121 TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG
     3181 ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC
     3241 CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA
     3301 CCCGTGGGGC CGCCATGCCG GCGATAATGG CCTGCTTCTC GCCGAAACGT TTGGTGGCGG
     3361 GACCAGTGAC GAAGGCTTGA GCGAGGGCGT GCAAGATTCC GAATACCGCA AGCGACAGGC
     3421 CGATCATCGT CGCGCTCCAG CGAAAGCGGT CCTCGCCGAA AATGACCCAG AGCGCTGCCG
     3481 GCACCTGTCC TACGAGTTGC ATGATAAAGA AGACAGTCAT AAGTGCGGCG ACGATAGTCA
     3541 TGCCCCGCGC CCACCGGAAG GAGCTGACTG GGTTGAAGGC TCTCAAGGGC ATCGGTCGAG
     3601 ATCCCGGTGC CTAATGAGTG AGCTAACTTA CATTAATTGC GTTGCGCTCA CTGCCCGCTT
     3661 TCCAGTCGGG AAACCTGTCG TGCCAGCTGC ATTAATGAAT CGGCCAACGC GCGGGGAGAG
     3721 GCGGTTTGCG TATTGGGCGC CAGGGTGGTT TTTCTTTTCA CCAGTGAGAC GGGCAACAGC
     3781 TGATTGCCCT TCACCGCCTG GCCCTGAGAG AGTTGCAGCA AGCGGTCCAC GCTGGTTTGC
     3841 CCCAGCAGGC GAAAATCCTG TTTGATGGTG GTTAACGGCG GGATATAACA TGAGCTGTCT
     3901 TCGGTATCGT CGTATCCCAC TACCGAGATG TCCGCACCAA CGCGCAGCCC GGACTCGGTA
     3961 ATGGCGCGCA TTGCGCCCAG CGCCATCTGA TCGTTGGCAA CCAGCATCGC AGTGGGAACG
     4021 ATGCCCTCAT TCAGCATTTG CATGGTTTGT TGAAAACCGG ACATGGCACT CCAGTCGCCT
     4081 TCCCGTTCCG CTATCGGCTG AATTTGATTG CGAGTGAGAT ATTTATGCCA GCCAGCCAGA
     4141 CGCAGACGCG CCGAGACAGA ACTTAATGGG CCCGCTAACA GCGCGATTTG CTGGTGACCC
     4201 AATGCGACCA GATGCTCCAC GCCCAGTCGC GTACCGTCTT CATGGGAGAA AATAATACTG
     4261 TTGATGGGTG TCTGGTCAGA GACATCAAGA AATAACGCCG GAACATTAGT GCAGGCAGCT
     4321 TCCACAGCAA TGGCATCCTG GTCATCCAGC GGATAGTTAA TGATCAGCCC ACTGACGCGT
     4381 TGCGCGAGAA GATTGTGCAC CGCCGCTTTA CAGGCTTCGA CGCCGCTTCG TTCTACCATC
     4441 GACACCACCA CGCTGGCACC CAGTTGATCG GCGCGAGATT TAATCGCCGC GACAATTTGC
     4501 GACGGCGCGT GCAGGGCCAG ACTGGAGGTG GCAACGCCAA TCAGCAACGA CTGTTTGCCC
     4561 GCCAGTTGTT GTGCCACGCG GTTGGGAATG TAATTCAGCT CCGCCATCGC CGCTTCCACT
     4621 TTTTCCCGCG TTTTCGCAGA AACGTGGCTG GCCTGGTTCA CCACGCGGGA AACGGTCTGA
     4681 TAAGAGACAC CGGCATACTC TGCGACATCG TATAACGTTA CTGGTTTCAC ATTCACCACC
     4741 CTGAATTGAC TCTCTTCCGG GCGCTATCAT GCCATACCGC GAAAGGTTTT GCGCCATTCG
     4801 ATGGTGTCCG GGATCTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG
     4861 TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC
     4921 GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT
     4981 GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC
     5041 AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT
     5101 CGATCTCGAT CCCGCGAAAT TAATACGACT CACTATAGGG GAATTGTGAG CGGATAACAA
     5161 TTCCCCTCTA GAAATAATTT TGTTTAACTT TAAGAAGGAG ATATACATAT GAGCGATAAA
     5221 ATTATTCACC TGACTGACGA CAGTTTTGAC ACGGATGTAC TCAAAGCGGA CGGGGCGATC
     5281 CTCGTCGATT TCTGGGCAGA GTGGTGCGGT CCGTGCAAAA TGATCGCCCC GATTCTGGAT
     5341 GAAATCGCTG ACGAATATCA GGGCAAACTG ACCGTTGCAA AACTGAACAT CGATCAAAAC
     5401 CCTGGCACTG CGCCGAAATA TGGCATCCGT GGTATCCCGA CTCTGCTGCT GTTCAAAAAC
     5461 GGTGAAGTGG CGGCAACCAA AGTGGGTGCA CTGTCTAAAG GTCAGTTGAA AGAGTTCCTC
     5521 GACGCTAACC TGGCCGGTTC TGGTTCTGGC CATATGCACC ATCATCATCA TCATTCTTCT
     5581 GGTCTGGTGC CACGCGGTTC TGGTATGAAA GAAACCGCTG CTGCTAAATT CGAACGCCAG
     5641 CACATGGACA GCCCAGATCT GGGTACCGAT GACGACGACA AGATGGCACT GAAGGACTAC
     5701 GCGCTAGAGA AGGAAAAGGT TAAGAAGTTC TTACAAGAGT TCTACCAGGA TGATGAACTC
     5761 GGGAAGAAGC AGTTCAAGTA TGGGAACCAG TTGGTTCGGC TGGCTCATCG GGAACAGGTG
     5821 GCTCTGTATG TGGACCTGGA CGACGTAGCC GAGGATGACC CCGAGTTGGT GGACTCAATT
     5881 TGTGAGAATG CCAGGCGCTA CGCGAAGCTC TTTGCTGATG CCGTACAAGA GCTGCTGCCT
     5941 CAGTACAAGG AGAGGGAAGT GGTAAATAAA GATGTCCTGG ACGTTTACAT TGAGCATCGG
     6001 CTAATGATGG AGCAGCGGAG TCGGGACCCT GGGATGGTCC GAAGCCCCCA GAACCAGTAC
     6061 CCTGCTGAAC TCATGCGCAG ATTTGAGCTG TATTTTCAAG GCCCTAGCAG CAACAAGCCT
     6121 CGTGTGATCC GGGAAGTGCG GGCTGACTCT GTGGGGAAGT TGGTAACTGT GCGTGGAATC
     6181 GTCACTCGTG TCTCTGAAGT CAAACCCAAG ATGGTGGTGG CCACTTACAC TTGTGACCAG
     6241 TGTGGGGCAG AGACCTACCA GCCGATCCAG TCTCCCACTT TCATGCCTCT GATCATGTGC
     6301 CCAAGCCAGG AGTGCCAAAC CAACCGCTCA GGAGGGCGGC TGTATCTGCA GACACGGGGC
     6361 TCCAGATTCA TCAAATTCCA GGAGATGAAG ATGCAAGAAC ATAGTGATCA GGTGCCTGTG
     6421 GGAAATATCC CTCGTAGTAT CACGGTGCTG GTAGAAGGAG AGAACACAAG GATTGCCCAG
     6481 CCTGGAGACC ACGTCAGCGT CACTGGTATT TTCTTGCCAA TCCTGCGCAC TGGGTTCCGA
     6541 CAGGTGGTAC AGGGTTTACT CTCAGAAACC TACCTGGAAG CCCATCGGAT TGTGAAGATG
     6601 AACAAGAGTG AGGATGATGA GTCTGGGGCT GGAGAGCTCA CCAGGGAGGA GCTGAGGCAA
     6661 ATTGCAGAGG AGGATTTCTA CGAAAAGCTG GCAGCTTCAA TCGCCCCAGA AATATACGGG
     6721 CATGAAGATG TGAAGAAGGC ACTGCTGCTC CTGCTAGTCG GGGGTGTGGA CCAGTCTCCT
     6781 CGAGGCATGA AAATCCGGGG CAACATCAAC ATCTGTCTGA TGGGGGATCC TGGTGTGGCC
     6841 AAGTCTCAGC TCCTGTCATA CATTGATCGA CTGGCGCCTC GCAGCCAGTA CACAACAGGC
     6901 CGGGGCTCCT CAGGAGTGGG GCTTACGGCA GCTGTGCTGA GAGACTCCGT GAGTGGAGAA
     6961 CTGACCTTAG AGGGTGGGGC CCTGGTGCTG GCTGACCAGG GTGTGTGCTG CATTGATGAG
     7021 TTCGACAAGA TGGCTGAGGC CGACCGCACA GCCATCCACG AGGTCATGGA GCAGCAGACC
     7081 ATCTCCATTG CCAAGGCCGG CATTCTCACC ACACTCAATG CCCGCTGCTC CATCCTGGCT
     7141 GCCGCCAACC CTGCCTACGG GCGCTACAAC CCTCGCCGCA GCCTGGAGCA GAACATACAG
     7201 CTACCTGCTG CACTGCTCTC CCGGTTTGAC CTCCTCTGGC TGATTCAGGA CCGGCCCGAC
     7261 CGAGACAATG ACCTACGGTT GGCCCAGCAC ATCACCTATG TGCACCAGCA CAGCCGGCAG
     7321 CCCCCCTCCC AGTTTGAACC TCTGGACATG AAGCTCATGA GGCGTTACAT AGCCATGTGC
     7381 CGCGAGAAGC AGCCCATGGT GCCAGAGTCT CTGGCTGACT ACATCACAGC AGCATACGTG
     7441 GAGATGAGGC GAGAGGCTTG GGCTAGTAAG GATGCCACCT ATACTTCTGC CCGGACCCTG
     7501 CTGGCTATCC TGCGCCTTTC CACTGCTCTG GCACGTCTGA GAATGGTGGA TGTGGTGGAG
     7561 AAAGAAGATG TGAATGAAGC CATCAGGCTA ATGGAGATGT CAAAGGACTC TCTTCTAGGA
     7621 GACAAGGGGC AGACAGCTAG GACTCAGAGA CCAGCAGATG TGATATTTGC CACCGTCCGT
     7681 GAACTGGTCT CAGGGGGCCG AAGTGTCCGG TTCTCTGAGG CAGAGCAGCG CTGTGTATCT
     7741 CGTGGCTTCA CACCCGCCCA GTTCCAGGCG GCTCTGGATG AATATGAGGA GCTCAATGTC
     7801 TGGCAGGTCA ATGCTTCCCG GACACGGATC ACTTTTGTCT GACGGCCGCC CGCGTTGATC
     7861 TCGATCCCGC GAAATTAATA CGACTCACTA TAGGGGAATT GTGAGCGGAT AACAATTCCC
     7921 CTCTAGAAAT AATTTTGTTT AACTTTAAGA AGGAGATATA CCATGCAAGC GGGCCCGGCC
     7981 TCCATGGCGG AATCATCGGA ATCCTTCACC ATGGCATCCA GCCCGGCCCA GCGTCGGCGA
     8041 GGCAATGATC CTCTCACCTC CAGCCCTGGC CGAAGCTCCC GGCGTACTGA TGCCCTCACC
     8101 TCCAGCCCTG GCCGTGACCT TCCACCATTT GAGGATGAGT CCGAGGGGCT CCTAGGCACA
     8161 GAGGGGCCCC TGGAGGAAGA AGAGGATGGA GAGGAGCTCA TTGGAGATGG CATGGAAAGG
     8221 GACTACCGCG CCATCCCAGA GCTGGACGCC TATGAGGCCG AGGGACTGGC TCTGGATGAT
     8281 GAGGACGTAG AGGAGCTGAC GGCCAGTCAG AGGGAGGCAG CAGAGCGGGC CATGCGGCAG
     8341 CGTGACCGGG AGGCTGGCCG GGGCCTGGGC CGCATGCGCC GTGGGCTCCT GTATGACAGC
     8401 GATGAGGAGG ACGAGGAGCG CCCTGCCCGC AAGCGCCGCC AGGTGGAGCG GGCCACGGAG
     8461 GACGGCGAGG AGGACGAGGA GATGATCGAG AGCATCGAGA ACCTGGAGGA TCTCAAAGGC
     8521 CACTCTGTGC GCGAGTGGGT GAGCATGGCG GGCCCCCGGC TGGAGATCCA CCACCGCTTC
     8581 AAGAACTTCC TGCGCACTCA CGTCGACAGC CACGGCCACA ACGTCTTCAA GGAGCGCATC
     8641 AGCGACATGT GCAAAGAGAA CCGTGAGAGC CTGGTGGTGA ACTATGAGGA CTTGGCAGCC
     8701 AGGGAGCACG TGCTGGCCTA CTTCCTGCCT GAGGCACCGG CGGAGCTGCT GCAGATCTTT
     8761 GATGAGGCTG CCCTGGAGGT GGTACTGGCC ATGTACCCCA AGTACGACCG CATCACCAAC
     8821 CACATCCATG TCCGCATCTC CCACCTGCCT CTGGTGGAGG AGCTGCGCTC GCTGAGGCAG
     8881 CTGCATCTGA ACCAGCTGAT CCGCACCAGT GGGGTGGTGA CCAGCTGCAC TGGCGTCCTG
     8941 CCCCAGCTCA GCATGGTCAA GTACAACTGC AACAAGTGCA ATTTCGTCCT GGGTCCTTTC
     9001 TGCCAGTCCC AGAACCAGGA GGTGAAACCA GGCTCCTGTC CTGAGTGCCA GTCGGCCGGC
     9061 CCCTTTGAGG TCAACATGGA GGAGACCATC TATCAGAACT ACCAGCGTAT CCGAATCCAG
     9121 GAGAGTCCAG GCAAAGTGGC GGCTGGCCGG CTGCCCCGCT CCAAGGACGC CATTCTCCTC
     9181 GCAGATCTGG TGGACAGCTG CAAGCCAGGA GACGAGATAG AGCTGACTGG CATCTATCAC
     9241 AACAACTATG ATGGCTCCCT CAACACTGCC AATGGCTTCC CTGTCTTTGC CACTGTCATC
     9301 CTAGCCAACC ACGTGGCCAA GAAGGACAAC AAGGTTGCTG TAGGGGAACT GACCGATGAA
     9361 GATGTGAAGA TGATCACTAG CCTCTCCAAG GATCAGCAGA TCGGAGAGAA GATCTTTGCC
     9421 AGCATTGCTC CTTCCATCTA TGGTCATGAA GACATCAAGA GAGGCCTGGC TCTGGCCCTG
     9481 TTCGGAGGGG AGCCCAAAAA CCCAGGTGGC AAGCACAAGG TACGTGGTGA TATCAACGTG
     9541 CTCTTGTGCG GAGACCCTGG CACAGCGAAG TCGCAGTTTC TCAAGTATAT TGAGAAAGTG
     9601 TCCAGCCGAG CCATCTTCAC CACTGGCCAG GGGGCGTCGG CTGTGGGCCT CACGGCGTAT
     9661 GTCCAGCGGC ACCCTGTCAG CAGGGAGTGG ACCTTGGAGG CTGGGGCCCT GGTTCTGGCT
     9721 GACCGAGGAG TGTGTCTCAT TGATGAATTT GACAAGATGA ATGACCAGGA CAGAACCAGC
     9781 ATCCATGAGG CCATGGAGCA ACAGAGCATC TCCATCTCGA AGGCTGGCAT CGTCACCTCC
     9841 CTGCAGGCTC GCTGCACGGT CATTGCTGCC GCCAACCCCA TAGGAGGGCG CTACGACCCC
     9901 TCGCTGACTT TCTCTGAGAA CGTGGACCTC ACAGAGCCCA TCATCTCACG CTTTGACATC
     9961 CTGTGTGTGG TGAGGGACAC CGTGGACCCA GTCCAGGACG AGATGCTGGC CCGCTTCGTG
    10021 GTGGGCAGCC ACGTCAGACA CCACCCCAGC AACAAGGAGG AGGAGGGGCT GGCCAATGGC
    10081 AGCGCTGCTG AGCCCGCCAT GCCCAACACG TATGGCGTGG AGCCCCTGCC CCAGGAGGTC
    10141 CTGAAGAAGT ACATCATCTA CGCCAAGGAG AGGGTCCACC CGAAGCTCAA CCAGATGGAC
    10201 CAGGACAAGG TGGCCAAGAT GTACAGTGAC CTGAGGAAAG AATCTATGGC GACAGGCAGC
    10261 ATCCCCATTA CGGTGCGGCA CATCGAGTCC ATGATCCGCA TGGCGGAGGC CCACGCGCGC
    10321 ATCCATCTGC GGGACTATGT GATCGAAGAC GACGTCAACA TGGCCATCCG CGTGATGCTG
    10381 GAGAGCTTCA TAGACACACA GAAGTTCAGC GTCATGCGCA GCATGCGCAA GACTTTTGCC
    10441 CGCTACCTTT CATTCCGGCG TGACAACAAT GAGCTGTTGC TCTTCATACT GAAGCAGTTA
    10501 GTGGCAGAGC AGGTGACATA TCAGCGCAAC CGCTTTGGGG CCCAGCAGGA CACTATTGAG
    10561 GTCCCTGAGA AGGACTTGGT GGATAAGGCT CGTCAGATCA ACATCCACAA CCTCTCTGCA
    10621 TTTTATGACA GTGAGCTCTT CAGGATGAAC AAGTTCAGCC ACGACCTGAA AAGGAAAATG
    10681 ATCCTGCAGC AGTTCTGACC GGGCTTCTCC TCAACCATGG CGATATCGGA TCCGAATTCG
    10741 AGCTCCGTCG ACAAGCTTGC GGCCGCACTC GAGCACCACC ACCACCACCA CTGAGATCCG
    10801 GCTGCTAACA AAGCCCGAAA GGAAGCTGAG TTGGCTGCTG CCACCGCTGA GCAATAACTA
    10861 GCATAACCCC TTGGGGCCTC TAAACGGGTC TTGAGGGGTT TTTTGCTGAA AGGAGGAACT
    10921 ATATCCGGAT
//
