LOCUS       pFS_0235-pET30-F        7290 bp    DNA     circular UNA 25-AUG-2018
DEFINITION  Concatenation of 5 sequences.
ACCESSION   urn.local...1z-9fa11gb
VERSION     urn.local...1z-9fa11gb
KEYWORDS    .
SOURCE      
  ORGANISM  
            .
FEATURES             Location/Qualifiers
     unsure          109
                     /created_by="User"
                     /label="T->C"
     promoter        180..208
                     /Imported_from="<a
                     href=""http://wishart.biology.ualberta.ca/PlasMapper"">Pla
                     sMapper</a>"
                     /Transferred_From="amp prom"
                     /Transferred_Similarity="89.66%"
                     /label="amp prom"
     marker          complement(211..1026)
                     /Imported_from="<a
                     href=""http://wishart.biology.ualberta.ca/PlasMapper"">Pla
                     sMapper</a>"
                     /Transferred_From="kan2 marker"
                     /Transferred_Similarity="99.75%"
                     /label="kan2 marker"
     misc_feature    complement(212..1026)
                     /note="KanR"
                     /label="KanR"
     misc_feature    1132..1751
                     /Imported_from="<a
                     href=""http://wishart.biology.ualberta.ca/PlasMapper"">Pla
                     sMapper</a>"
                     /Transferred_From="pBR322 origin"
                     /Transferred_Similarity="99.68%"
                     /label="pBR322 origin"
                     /note="Geneious type: origin of replication"
     unsure          1771
                     /created_by="User"
                     /label="C->T"
     unsure          1971^1972
                     /created_by="User"
                     /label="TAT del"
     gene            complement(2163..2354)
                     /Imported_from="<a
                     href=""http://wishart.biology.ualberta.ca/PlasMapper"">Pla
                     sMapper</a>"
                     /Transferred_From="ROP"
                     /Transferred_Similarity="100.00%"
                     /label="ROP"
     misc_feature    complement(3163..4254)
                     /Imported_from="<a
                     href=""http://wishart.biology.ualberta.ca/PlasMapper"">Pla
                     sMapper</a>"
                     /Transferred_From="lacI reg"
                     /Transferred_Similarity="99.91%"
                     /label="lacI reg"
                     /note="Geneious type: regulatory sequence"
     promoter        4636..4654
                     /Imported_from="<a
                     href=""http://wishart.biology.ualberta.ca/PlasMapper"">Pla
                     sMapper</a>"
                     /Transferred_From="T7 prom"
                     /Transferred_Similarity="100.00%"
                     /label="T7 prom"
     misc_feature    4654..4681
                     /Imported_from="<a
                     href=""http://wishart.biology.ualberta.ca/PlasMapper"">Pla
                     sMapper</a>"
                     /Transferred_From="lacO reg"
                     /Transferred_Similarity="100.00%"
                     /label="lacO reg"
                     /note="Geneious type: regulatory sequence"
     gene            4699..4715
                     /Imported_from="<a
                     href=""http://wishart.biology.ualberta.ca/PlasMapper"">Pla
                     sMapper</a>"
                     /Transferred_From="T7_transl_en_RBS"
                     /Transferred_Similarity="100.00%"
                     /label="T7_transl_en_RBS"
     CDS             4724..6622
                     /created_by="User"
                     /label="Cas1"
     gene            6629..6645
                     /Imported_from="<a
                     href=""http://wishart.biology.ualberta.ca/PlasMapper"">Pla
                     sMapper</a>"
                     /X-Transferred_From="Digestion of
                     pFSNW_013_pET30bvectorNovagen_Golden Gate_T7_Terminator -
                     Fragment 2; Ligated sequences; Ligated sequences 2;
                     T7_transl_en_RBS"
                     /X-Transferred_Similarity="100.00%"
                     /Transferred_From="Digestion of
                     pFSNW_013_pET30bvectorNovagen_Golden Gate_T7_Terminator -
                     Fragment 2, Ligated sequences, Ligated sequences 2"
                     /Transferred_Similarity="100.00%"
                     /label="T7_transl_en_RBS"
     CDS             6654..6914
                     /created_by="User"
                     /label="Cas2"
     misc_feature    <6916..>6919
                     /note="XhoI"
                     /label="XhoI"
     terminator      6927..7055
                     /created_by="User"
                     /label="Terminator extended"
     terminator      6988..7031
                     /Imported_from="<a
                     href=""http://wishart.biology.ualberta.ca/PlasMapper"">Pla
                     sMapper</a>"
                     /Transferred_From="T7 term"
                     /Transferred_Similarity="97.73%"
                     /label="T7 term"
     misc_feature    complement(7056..>7061)
                     /label="CsiI"
     accession       <7064..>7283
                     /Transferred_From="NZ_CYYV01000002 - ARRAY 2"
                     /Transferred_Similarity="100.00%"
                     /label="CYYV01000002.1"
     source          <7064..>7283
                     /organism="Fusicatenibacter saccharivorans"
                     /mol_type="genomic DNA"
                     /strain="2789STDY5608849"
                     /isolation_source="faeces"
                     /db_xref="taxon:1150298"
                     /country="United Kingdom"
                     /collection_date=2013
                     /note="contig: SCcontig000002"
                     /Transferred_From="NZ_CYYV01000002 - ARRAY 2"
                     /Transferred_Similarity="100.00%"
                     /label="contig: SCcontig000002 source Fusicatenibacter
                     saccharivorans"
     misc_feature    7064..>7283
                     /created_by="User"
                     /Transferred_From="NZ_CYYV01000002, NZ_CYYV01000002 -
                     ARRAY 2"
                     /Transferred_Similarity="100.00%"
                     /label="ARRAY 2"
     repeat_region   7255..>7283
                     /inference="COORDINATES: alignment:crt:1.2; COORDINATES:
                     alignment:pilercr:v1.02"
                     /rpt_family="CRISPR"
                     /rpt_type="direct"
                     /rpt_unit_range="67767..67795"
                     /rpt_unit_seq="gtcgtactttacctaaaaggaattgaaac"
                     /Transferred_From="NZ_CYYV01000002 - ARRAY 2"
                     /Transferred_Similarity="100.00%"
                     /label="repeat region"
     misc_feature    7255..7283
                     /created_by="User"
                     /Transferred_From="NZ_CYYV01000002, NZ_CYYV01000002 -
                     ARRAY 2"
                     /Transferred_Similarity="100.00%"
                     /label="DR 2"
     source          <7284..>7288
                     /organism="Fusicatenibacter saccharivorans"
                     /mol_type="genomic DNA"
                     /strain="2789STDY5608849"
                     /isolation_source="faeces"
                     /db_xref="taxon:1150298"
                     /country="United Kingdom"
                     /collection_date=2013
                     /note="contig: SCcontig000002"
                     /Transferred_From="NZ_CYYV01000002 - ARRAY 1"
                     /Transferred_Similarity="100.00%"
                     /label="contig: SCcontig000002 source Fusicatenibacter
                     saccharivorans"
ORIGIN      
        1 ggccgctatt cttttgattt ataagggatt ttgccgattt cggcctattg gttaaaaaat
       61 gagctgattt aacaaaaatt taacgcgaat tttaacaaaa tattaacgct tacaatttca
      121 ggtggcactt ttcggggaaa tgtgcgcgga acccctattt gtttattttt ctaaatacat
      181 tcaaatatgt atccgctcat gaattaattc ttagaaaaac tcatcgagca tcaaatgaaa
      241 ctgcaattta ttcatatcag gattatcaat accatatttt tgaaaaagcc gtttctgtaa
      301 tgaaggagaa aactcaccga ggcagttcca taggatggca agatcctggt atcggtctgc
      361 gattccgact cgtccaacat caatacaacc tattaatttc ccctcgtcaa aaataaggtt
      421 atcaagtgag aaatcaccat gagtgacgac tgaatccggt gagaatggca aaagtttatg
      481 catttctttc cagacttgtt caacaggcca gccattacgc tcgtcatcaa aatcactcgc
      541 atcaaccaaa ccgttattca ttcgtgattg cgcctgagcg agacgaaata cgcgatcgct
      601 gttaaaagga caattacaaa caggaatcga atgcaaccgg cgcaggaaca ctgccagcgc
      661 atcaacaata ttttcacctg aatcaggata ttcttctaat acctggaatg ctgttttccc
      721 ggggatcgca gtggtgagta accatgcatc atcaggagta cggataaaat gcttgatggt
      781 cggaagaggc ataaattccg tcagccagtt tagtctgacc atctcatctg taacatcatt
      841 ggcaacgcta cctttgccat gtttcagaaa caactctggc gcatcgggct tcccatacaa
      901 tcgatagatt gtcgcacctg attgcccgac attatcgcga gcccatttat acccatataa
      961 atcagcatcc atgttggaat ttaatcgcgg cctagagcaa gacgtttccc gttgaatatg
     1021 gctcataaca ccccttgtat tactgtttat gtaagcagac agttttattg ttcatgacca
     1081 aaatccctta acgtgagttt tcgttccact gagcgtcaga ccccgtagaa aagatcaaag
     1141 gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac
     1201 cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa
     1261 ctggcttcag cagagcgcag ataccaaata ctgtccttct agtgtagccg tagttaggcc
     1321 accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag
     1381 tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac
     1441 cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc
     1501 gaacgaccta caccgaactg agatacctac agcgtgagct atgagaaagc gccacgcttc
     1561 ccgaagggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca
     1621 cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc
     1681 tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg
     1741 ccagcaacgc ggccttttta cggttcctgg tcttttgctg gccttttgct cacatgttct
     1801 ttcctgcgtt atcccctgat tctgtggata accgtattac cgcctttgag tgagctgata
     1861 ccgctcgccg cagccgaacg accgagcgca gcgagtcagt gagcgaggaa gcggaagagc
     1921 gcctgatgcg gtattttctc cttacgcatc tgtgcggtat ttcacaccgc aatggtgcac
     1981 tctcagtaca atctgctctg atgccgcata gttaagccag tatacactcc gctatcgcta
     2041 cgtgactggg tcatggctgc gccccgacac ccgccaacac ccgctgacgc gccctgacgg
     2101 gcttgtctgc tcccggcatc cgcttacaga caagctgtga ccgtctccgg gagctgcatg
     2161 tgtcagaggt tttcaccgtc atcaccgaaa cgcgcgaggc agctgcggta aagctcatca
     2221 gcgtggtcgt gaagcgattc acagatgtct gcctgttcat ccgcgtccag ctcgttgagt
     2281 ttctccagaa gcgttaatgt ctggcttctg ataaagcggg ccatgttaag ggcggttttt
     2341 tcctgtttgg tcactgatgc ctccgtgtaa gggggatttc tgttcatggg ggtaatgata
     2401 ccgatgaaac gagagaggat gctcacgata cgggttactg atgatgaaca tgcccggtta
     2461 ctggaacgtt gtgagggtaa acaactggcg gtatggatgc ggcgggacca gagaaaaatc
     2521 actcagggtc aatgccagcg cttcgttaat acagatgtag gtgttccaca gggtagccag
     2581 cagcatcctg cgatgcagat ccggaacata atggtgcagg gcgctgactt ccgcgtttcc
     2641 agactttacg aaacacggaa accgaagatc attcatgttg ttgctcaggt cgcagacgtt
     2701 ttgcagcagc agtcgcttca cgttcgctcg cgtatcggtg attcattctg ctaaccagta
     2761 aggcaacccc gccagcctag ccgggtcctc aacgacagga gcacgatcat gcgcacccgt
     2821 ggggccgcca tgccggcgat aatggcctgc ttctcgccga aacgtttggt ggcgggacca
     2881 gtgacgaagg cttgagcgag ggcgtgcaag attccgaata ccgcaagcga caggccgatc
     2941 atcgtcgcgc tccagcgaaa gcggtcctcg ccgaaaatga cccagagcgc tgccggcacc
     3001 tgtcctacga gttgcatgat aaagaagata gtcataagtg cggcgacgat agtcatgccc
     3061 cgcgcccacc ggaaggagct gactgggttg aaggctctca agggcatcgg tcgagatccc
     3121 ggtgcctaat gagtgagcta acttacatta attgcgttgc gctcactgcc cgctttccag
     3181 tcgggaaacc tgtcgtgcca gctgcattaa tgaatcggcc aacgcgcggg gagaggcggt
     3241 ttgcgtattg ggcgccaggg tggtttttct tttcaccagt gagacgggca acagctgatt
     3301 gcccttcacc gcctggccct gagagagttg cagcaagcgg tccacgctgg tttgccccag
     3361 caggcgaaaa tcctgtttga tggtggttaa cggcgggata taacatgagc tatcttcggt
     3421 atcgtcgtat cccactaccg agatgtccgc accaacgcgc agcccggact cggtaatggc
     3481 gcgcattgcg cccagcgcca tctgatcgtt ggcaaccagc atcgcagtgg gaacgatgcc
     3541 ctcattcagc atttgcatgg tttgttgaaa accggacatg gcactccagt cgccttcccg
     3601 ttccgctatc ggctgaattt gattgcgagt gagatattta tgccagccag ccagacgcag
     3661 acgcgccgag acagaactta atgggcccgc taacagcgcg atttgctggt gacccaatgc
     3721 gaccagatgc tccacgccca gtcgcgtacc atcttcatgg gagaaaataa tactgttgat
     3781 gggtgtctgg tcagagacat caagaaataa cgccggaaca ttagtgcagg cagcttccac
     3841 agcaatggca tcctggtcat ccagcggata gttaatgatc agcccactga cgcgttgcgc
     3901 gagaagattg tgcaccgccg ctttacaggc ttcgacgccg cttcgttcta ccatcgacac
     3961 caccacgctg gcacccagtt gatcggcgcg agatttaatc gccgcgacaa tttgcgacgg
     4021 cgcgtgcagg gccagactgg aggtggcaac gccaatcagc aacgactgtt tgcccgccag
     4081 ttgttgtgcc acgcggttgg gaatgtaatt cagctccgcc atcgccgctt ccactttttc
     4141 ccgcgttttc gcagaaacgt ggctggcctg gttcaccacg cgggaaacgg tctgataaga
     4201 gacaccggca tactctgcga catcgtataa cgttactggt ttcacattca ccaccctgaa
     4261 ttgactctct tccgggcgct atcatgccat accgcgaaag gttttgcgcc attcgatggt
     4321 gtccgggatc tcgacgctct cccttatgcg actcctgcat taggaagcag cccagtagta
     4381 ggttgaggcc gttgagcacc gccgccgcaa ggaatggtgc atgcaaggag atggcgccca
     4441 acagtccccc ggccacgggg cctgccacca tacccacgcc gaaacaagcg ctcatgagcc
     4501 cgaagtggcg agcccgatct tccccatcgg tgatgtcggc gatataggcg ccagcaaccg
     4561 cacctgtggc gccggtgatg ccggccacga tgcgtccggc gtagaggatc gagatcgatc
     4621 tcgatcccgc gaaattaata cgactcacta taggggaatt gtgagcggat aacaattccc
     4681 ctctagaaat aattttgttt aactttaaga aggagatata catatgttca ctatagacga
     4741 gatgctatca aagaacaacc aaagattggc tttcgagcac ttcgctacta agaacgacgg
     4801 atgtggccct gatggtatgc acgtatctga gctggagaaa tattggcgta tgaatcacga
     4861 tcagatcatc tccgacttga agaaccaaga gtaccaaccc ggcattattt tgattagaga
     4921 acacatgaac aaaacaggta agagaaggaa cattgcatct ctcaacgtga tcgacaggtt
     4981 cataactcga ctgctgtcgc aaaagttgaa tagatactta gcccccattt tctgcgagaa
     5041 ctcatacgct taccaagact caaaaggggt gatgcccgcc gtactaaaag ccaaggagta
     5101 cgtcgagttg ggtatgagac acgttatcga gatagacctc aagaattact tcgacacgat
     5161 cccgttagag aacttaatcc cagagatcga gcgttacata acagatgagg cagtgctgca
     5221 cttgattaaa cagtacttat tctgtgacat ctcgttcgag ggtaagatat cacgtaaaac
     5281 tcaagggatc gttcagggta acgcaatttc cccaatacta tccaacctct acctgaacga
     5341 cttcgacaag gagctggacg agtcgaagct gtgctggatc agatacgccg acaacatata
     5401 catctatatg gacagctacg agaaggccct actggtttat tccgagctta ccgagagact
     5461 tgagagaagg aaattaaccg ttaacaagga gaaatcaggg gtgtttgatg ttagtacccg
     5521 ttctatacta ggttacgaca tcctgatcag aaacaagaag gttgacgtgc gtaaacacat
     5581 ctacaagtct gttaaccagt actccaactg gcacgactct cgattggagt tcatcaacgg
     5641 tcgttaccac atcacttccg acggtatcct caatagacaa gacttcggtc tactgttcga
     5701 gaatgagcag aagaagcact acatacctgt tgaggtttct gaccagttga atatttacgg
     5761 aaacgttacg ctcgccagca acgttcttca atcattctcc aaccgagaga tcaaagtgtc
     5821 cttcttcgac aagtacggga gacttattgg atctttcctg cccgagaaaa ctaagaaaag
     5881 cgccgagata atactggttc agtctaagaa ctaccttaac gaggacgtta ggatggacac
     5941 ggcccgacgt atggagatcg ccggtttgca taacatcaga gccaacctca gatactacga
     6001 caagaagcac aaaggtgact ttaaggaaaa ggtagacgcc atttctggct atattgacgc
     6061 cctcaataga gcaccatccg tgaacgacat gatgctctta gaggctaaag ctcgtcagct
     6121 gtactacaca tgcttcaacc agatcttaga gacatccgac tttcagttcg agaagcgtac
     6181 caagcgacca ccaaaagacg ccatcaacgc ctgtatttca ttcggcaaca cattactgta
     6241 caacttgttt gtgaatatca tttggaagaa gggacttgat ccaaggttcg gggttgttca
     6301 cgccagcaac aagcgtaatc agtcccttaa cctggatttt gctgacatct tcaagcctat
     6361 agtcatagat aggatcatct tcacaatgat caacaagaag atgctgacct tgttgacgga
     6421 cttcgagact tctaaccagg gagtttacct ttcccgagag gggaagaaca tcttcttgca
     6481 gatgtacgaa gaaaagctca aatccagaat cactatcaag ggaaaggaga tgagttacta
     6541 tcagttgcta gaatcagagg tgcagaacta caagaacttc atcctgaccg gcgagacata
     6601 caagccgtac aagtactact agcgtacgtt aactttaaga aggagaactt aagatgtatg
     6661 tcattctagt ttacgacatt caccagaagc gagtaggtaa ggctctaaag atttgtagga
     6721 agtacctgat ccacatccag aagtcagttt tcgaaggcaa cataacggag agtaagctaa
     6781 aggccttaaa agaggagctg gggcacctga tcgacactca gatggactcg gtaattatct
     6841 accacctgga ctccgtgaag tacactaaga aggagcagat cggcatcgtc caatcgacat
     6901 ccaatgtcat ttgactcgag atccggctgc taacaaagcc cgaaaggaag ctgagttggc
     6961 tgctgccacc gctgagcaat aactagcata accccttggg gcctctaaac gggtcttgag
     7021 gggttttttg ctgaaaggag gaactatatc cggataccag gtgcggggaa gcttatgttc
     7081 catagcaaaa agtcggtcag tctcgtggct gaaatcatga gttccacaaa atggctgaaa
     7141 ttcaaggaaa atcaggaatc tcagaaaaac gatcgaccga ctttttcgat aaaatggttg
     7201 caaaaatgag aaaaatctga tttaatagaa tctgaaaaca gcggaaatgc tgttgtcgta
     7261 ctttacctaa aaggaattga aacgtcccgc
//
