LOCUS       pFS_0272_pET30_p        9296 bp    DNA     circular UNA 25-AUG-2018
DEFINITION  Concatenation of 2 sequences.
ACCESSION   urn.local...21-9fa11gb
VERSION     urn.local...21-9fa11gb
KEYWORDS    .
SOURCE      
  ORGANISM  
            .
FEATURES             Location/Qualifiers
     promoter        180..208
                     /Imported_from="<a
                     href=""http://wishart.biology.ualberta.ca/PlasMapper"">Pla
                     sMapper</a>"
                     /Transferred_From="amp prom"
                     /Transferred_Similarity="89.66%"
                     /label="amp prom"
     marker          complement(211..1026)
                     /Imported_from="<a
                     href=""http://wishart.biology.ualberta.ca/PlasMapper"">Pla
                     sMapper</a>"
                     /Transferred_From="kan2 marker"
                     /Transferred_Similarity="99.75%"
                     /label="kan2 marker"
     misc_feature    complement(212..1026)
                     /note="KanR"
                     /label="KanR"
     misc_feature    1132..1751
                     /Imported_from="<a
                     href=""http://wishart.biology.ualberta.ca/PlasMapper"">Pla
                     sMapper</a>"
                     /Transferred_From="pBR322 origin"
                     /Transferred_Similarity="99.68%"
                     /label="pBR322 origin"
                     /note="Geneious type: origin of replication"
     gene            complement(2163..2354)
                     /Imported_from="<a
                     href=""http://wishart.biology.ualberta.ca/PlasMapper"">Pla
                     sMapper</a>"
                     /Transferred_From="ROP"
                     /Transferred_Similarity="100.00%"
                     /label="ROP"
     misc_feature    complement(3163..4254)
                     /Imported_from="<a
                     href=""http://wishart.biology.ualberta.ca/PlasMapper"">Pla
                     sMapper</a>"
                     /Transferred_From="lacI reg"
                     /Transferred_Similarity="99.91%"
                     /label="lacI reg"
                     /note="Geneious type: regulatory sequence"
     promoter        complement(4246..4323)
                     /gene="lacI"
                     /note="; lacI promoter"
                     /Transferred_From="lacI promoter"
                     /Transferred_Similarity="100.00%"
                     /label="lacI promoter"
     misc_feature    4425..>4429
                     /created_by="User"
                     /label
     protein_bind    4431..4468
                     /created_by="User"
                     /modified_by="User"
                     /label="operator: P(Cat)"
     misc_feature    <4431..>5353
                     /note="sequenced"
                     /note=150219
                     /note="3_pSUB36part c2_oLP218"
                     /label="3 pSUB36part c2 oLP218"
     -35_signal      4434..4439
                     /note
                     /label="-35 signal"
     -10_signal      4457..4462
                     /note
                     /label="-10 signal"
     RBS             4477..4488
                     /note="B0043"
                     /label="B0043 RBS"
     gene            4495..5115
                     /note="tetR"
                     /label="tetR gene"
     terminator      5166..5209
                     /note
                     /label="terminator"
     terminator      5250..5269
                     /note="T7 TE"
                     /label="T7 TE terminator"
     protein_bind    5292..5311
                     /note="tetR binding site"
                     /label="tetR binding site protein bind"
     promoter        5292..5349
                     /note="P_tetA"
                     /label="P tetA promoter"
     -35_signal      5311..5316
                     /note
                     /label="-35 signal"
     protein_bind    5317..5336
                     /note="tetR binding site"
                     /label="tetR binding site protein bind"
     -10_signal      5334..5339
                     /note
                     /label="-10 signal"
     misc_feature    5350..5353
                     /label="GGC_overhang"
     misc_feature    5354..5373
                     /label="18k sRBS"
     CDS             5374..6309
                     /gene="Rluc"
                     /label="Rluc CDS"
     misc_feature    5374..6309
                     /label="Rluc"
     misc_feature    complement(6310..6312)
                     /label="1xStop_codon"
     misc_feature    complement(<6313..6316)
                     /label="Golden_Gate"
     misc_feature    6318..6419
                     /created_by="User"
                     /label
     terminator      complement(6331..6417)
                     /gene="Escherichia coli rrnB"
                     /note="rrnB T1 terminator"
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
                     /label="Escherichia coli rrnB terminator"
     promoter        6642..6660
                     /Imported_from="<a
                     href=""http://wishart.biology.ualberta.ca/PlasMapper"">Pla
                     sMapper</a>"
                     /Transferred_From="T7 prom"
                     /Transferred_Similarity="100.00%"
                     /label="T7 prom"
     misc_feature    6660..6687
                     /Imported_from="<a
                     href=""http://wishart.biology.ualberta.ca/PlasMapper"">Pla
                     sMapper</a>"
                     /Transferred_From="lacO reg"
                     /Transferred_Similarity="100.00%"
                     /label="lacO reg"
                     /note="Geneious type: regulatory sequence"
     protein_bind    complement(6661..6685)
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-beta-D-thiogalactopyranoside (IPTG).; lac
                     operator"
                     /Transferred_From="lac operator protein bind"
                     /Transferred_Similarity="100.00%"
                     /label="lac operator protein bind"
     gene            6705..6721
                     /Imported_from="<a
                     href=""http://wishart.biology.ualberta.ca/PlasMapper"">Pla
                     sMapper</a>"
                     /Transferred_From="T7_transl_en_RBS"
                     /Transferred_Similarity="100.00%"
                     /label="T7_transl_en_RBS"
     CDS             6730..8628
                     /created_by="User"
                     /label="Cas1"
     gene            8635..8651
                     /Imported_from="<a
                     href=""http://wishart.biology.ualberta.ca/PlasMapper"">Pla
                     sMapper</a>"
                     /X-Transferred_From="Digestion of
                     pFSNW_013_pET30bvectorNovagen_Golden Gate_T7_Terminator -
                     Fragment 2; Ligated sequences; Ligated sequences 2;
                     T7_transl_en_RBS"
                     /X-Transferred_Similarity="100.00%"
                     /Transferred_From="Digestion of
                     pFSNW_013_pET30bvectorNovagen_Golden Gate_T7_Terminator -
                     Fragment 2, Ligated sequences, Ligated sequences 2"
                     /Transferred_Similarity="100.00%"
                     /label="T7_transl_en_RBS"
     CDS             8660..8920
                     /created_by="User"
                     /label="Cas2"
     misc_feature    <8922..>8925
                     /note="XhoI"
                     /label="XhoI"
     terminator      8933..9061
                     /created_by="User"
                     /label="Terminator extended"
     terminator      8994..9037
                     /Imported_from="<a
                     href=""http://wishart.biology.ualberta.ca/PlasMapper"">Pla
                     sMapper</a>"
                     /Transferred_From="T7 term"
                     /Transferred_Similarity="97.73%"
                     /label="T7 term"
     misc_feature    complement(9062..>9067)
                     /label="CsiI"
     source          <9070..>9289
                     /organism="Fusicatenibacter saccharivorans"
                     /mol_type="genomic DNA"
                     /strain="2789STDY5608849"
                     /isolation_source="faeces"
                     /db_xref="taxon:1150298"
                     /country="United Kingdom"
                     /collection_date=2013
                     /note="contig: SCcontig000002"
                     /Transferred_From="NZ_CYYV01000002 - ARRAY 2"
                     /Transferred_Similarity="100.00%"
                     /label="contig: SCcontig000002 source Fusicatenibacter
                     saccharivorans"
     promoter        9070..9260
                     /created_by="User"
                     /label="Leader"
     repeat_region   9261..>9289
                     /inference="COORDINATES: alignment:crt:1.2; COORDINATES:
                     alignment:pilercr:v1.02"
                     /rpt_family="CRISPR"
                     /rpt_type="direct"
                     /rpt_unit_range="67767..67795"
                     /rpt_unit_seq="gtcgtactttacctaaaaggaattgaaac"
                     /Transferred_From="NZ_CYYV01000002 - ARRAY 2"
                     /Transferred_Similarity="100.00%"
                     /label="repeat region"
     misc_feature    9261..9289
                     /created_by="User"
                     /Transferred_From="NZ_CYYV01000002, NZ_CYYV01000002 -
                     ARRAY 2"
                     /Transferred_Similarity="100.00%"
                     /label="DR 2"
     source          <9290..>9294
                     /organism="Fusicatenibacter saccharivorans"
                     /mol_type="genomic DNA"
                     /strain="2789STDY5608849"
                     /isolation_source="faeces"
                     /db_xref="taxon:1150298"
                     /country="United Kingdom"
                     /collection_date=2013
                     /note="contig: SCcontig000002"
                     /Transferred_From="NZ_CYYV01000002 - ARRAY 1"
                     /Transferred_Similarity="100.00%"
                     /label="contig: SCcontig000002 source Fusicatenibacter
                     saccharivorans"
ORIGIN      
        1 ggccgctatt cttttgattt ataagggatt ttgccgattt cggcctattg gttaaaaaat
       61 gagctgattt aacaaaaatt taacgcgaat tttaacaaaa tattaacgct tacaatttca
      121 ggtggcactt ttcggggaaa tgtgcgcgga acccctattt gtttattttt ctaaatacat
      181 tcaaatatgt atccgctcat gaattaattc ttagaaaaac tcatcgagca tcaaatgaaa
      241 ctgcaattta ttcatatcag gattatcaat accatatttt tgaaaaagcc gtttctgtaa
      301 tgaaggagaa aactcaccga ggcagttcca taggatggca agatcctggt atcggtctgc
      361 gattccgact cgtccaacat caatacaacc tattaatttc ccctcgtcaa aaataaggtt
      421 atcaagtgag aaatcaccat gagtgacgac tgaatccggt gagaatggca aaagtttatg
      481 catttctttc cagacttgtt caacaggcca gccattacgc tcgtcatcaa aatcactcgc
      541 atcaaccaaa ccgttattca ttcgtgattg cgcctgagcg agacgaaata cgcgatcgct
      601 gttaaaagga caattacaaa caggaatcga atgcaaccgg cgcaggaaca ctgccagcgc
      661 atcaacaata ttttcacctg aatcaggata ttcttctaat acctggaatg ctgttttccc
      721 ggggatcgca gtggtgagta accatgcatc atcaggagta cggataaaat gcttgatggt
      781 cggaagaggc ataaattccg tcagccagtt tagtctgacc atctcatctg taacatcatt
      841 ggcaacgcta cctttgccat gtttcagaaa caactctggc gcatcgggct tcccatacaa
      901 tcgatagatt gtcgcacctg attgcccgac attatcgcga gcccatttat acccatataa
      961 atcagcatcc atgttggaat ttaatcgcgg cctagagcaa gacgtttccc gttgaatatg
     1021 gctcataaca ccccttgtat tactgtttat gtaagcagac agttttattg ttcatgacca
     1081 aaatccctta acgtgagttt tcgttccact gagcgtcaga ccccgtagaa aagatcaaag
     1141 gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac
     1201 cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa
     1261 ctggcttcag cagagcgcag ataccaaata ctgtccttct agtgtagccg tagttaggcc
     1321 accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag
     1381 tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac
     1441 cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc
     1501 gaacgaccta caccgaactg agatacctac agcgtgagct atgagaaagc gccacgcttc
     1561 ccgaagggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca
     1621 cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc
     1681 tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg
     1741 ccagcaacgc ggccttttta cggttcctgg tcttttgctg gccttttgct cacatgttct
     1801 ttcctgcgtt atcccctgat tctgtggata accgtattac cgcctttgag tgagctgata
     1861 ccgctcgccg cagccgaacg accgagcgca gcgagtcagt gagcgaggaa gcggaagagc
     1921 gcctgatgcg gtattttctc cttacgcatc tgtgcggtat ttcacaccgc aatggtgcac
     1981 tctcagtaca atctgctctg atgccgcata gttaagccag tatacactcc gctatcgcta
     2041 cgtgactggg tcatggctgc gccccgacac ccgccaacac ccgctgacgc gccctgacgg
     2101 gcttgtctgc tcccggcatc cgcttacaga caagctgtga ccgtctccgg gagctgcatg
     2161 tgtcagaggt tttcaccgtc atcaccgaaa cgcgcgaggc agctgcggta aagctcatca
     2221 gcgtggtcgt gaagcgattc acagatgtct gcctgttcat ccgcgtccag ctcgttgagt
     2281 ttctccagaa gcgttaatgt ctggcttctg ataaagcggg ccatgttaag ggcggttttt
     2341 tcctgtttgg tcactgatgc ctccgtgtaa gggggatttc tgttcatggg ggtaatgata
     2401 ccgatgaaac gagagaggat gctcacgata cgggttactg atgatgaaca tgcccggtta
     2461 ctggaacgtt gtgagggtaa acaactggcg gtatggatgc ggcgggacca gagaaaaatc
     2521 actcagggtc aatgccagcg cttcgttaat acagatgtag gtgttccaca gggtagccag
     2581 cagcatcctg cgatgcagat ccggaacata atggtgcagg gcgctgactt ccgcgtttcc
     2641 agactttacg aaacacggaa accgaagatc attcatgttg ttgctcaggt cgcagacgtt
     2701 ttgcagcagc agtcgcttca cgttcgctcg cgtatcggtg attcattctg ctaaccagta
     2761 aggcaacccc gccagcctag ccgggtcctc aacgacagga gcacgatcat gcgcacccgt
     2821 ggggccgcca tgccggcgat aatggcctgc ttctcgccga aacgtttggt ggcgggacca
     2881 gtgacgaagg cttgagcgag ggcgtgcaag attccgaata ccgcaagcga caggccgatc
     2941 atcgtcgcgc tccagcgaaa gcggtcctcg ccgaaaatga cccagagcgc tgccggcacc
     3001 tgtcctacga gttgcatgat aaagaagata gtcataagtg cggcgacgat agtcatgccc
     3061 cgcgcccacc ggaaggagct gactgggttg aaggctctca agggcatcgg tcgagatccc
     3121 ggtgcctaat gagtgagcta acttacatta attgcgttgc gctcactgcc cgctttccag
     3181 tcgggaaacc tgtcgtgcca gctgcattaa tgaatcggcc aacgcgcggg gagaggcggt
     3241 ttgcgtattg ggcgccaggg tggtttttct tttcaccagt gagacgggca acagctgatt
     3301 gcccttcacc gcctggccct gagagagttg cagcaagcgg tccacgctgg tttgccccag
     3361 caggcgaaaa tcctgtttga tggtggttaa cggcgggata taacatgagc tatcttcggt
     3421 atcgtcgtat cccactaccg agatgtccgc accaacgcgc agcccggact cggtaatggc
     3481 gcgcattgcg cccagcgcca tctgatcgtt ggcaaccagc atcgcagtgg gaacgatgcc
     3541 ctcattcagc atttgcatgg tttgttgaaa accggacatg gcactccagt cgccttcccg
     3601 ttccgctatc ggctgaattt gattgcgagt gagatattta tgccagccag ccagacgcag
     3661 acgcgccgag acagaactta atgggcccgc taacagcgcg atttgctggt gacccaatgc
     3721 gaccagatgc tccacgccca gtcgcgtacc atcttcatgg gagaaaataa tactgttgat
     3781 gggtgtctgg tcagagacat caagaaataa cgccggaaca ttagtgcagg cagcttccac
     3841 agcaatggca tcctggtcat ccagcggata gttaatgatc agcccactga cgcgttgcgc
     3901 gagaagattg tgcaccgccg ctttacaggc ttcgacgccg cttcgttcta ccatcgacac
     3961 caccacgctg gcacccagtt gatcggcgcg agatttaatc gccgcgacaa tttgcgacgg
     4021 cgcgtgcagg gccagactgg aggtggcaac gccaatcagc aacgactgtt tgcccgccag
     4081 ttgttgtgcc acgcggttgg gaatgtaatt cagctccgcc atcgccgctt ccactttttc
     4141 ccgcgttttc gcagaaacgt ggctggcctg gttcaccacg cgggaaacgg tctgataaga
     4201 gacaccggca tactctgcga catcgtataa cgttactggt ttcacattca ccaccctgaa
     4261 ttgactctct tccgggcgct atcatgccat accgcgaaag gttttgcgcc attcgatggt
     4321 gtccgggatc tcgacgctct cccttatgcg actcctgcat taggaagcag cccagtagta
     4381 ggttgaggcc gttgagcacc gccgccgcaa ggaatggtgc atgcaccggt ggcacgtaag
     4441 aggttccaac tttcaccata atgaaacata ctagagaaag aggagaaata ctagatgtcc
     4501 agattagata aaagtaaagt gattaacagc gcattagagc tgcttaatga ggtcggaatc
     4561 gaaggtttaa caacccgtaa actcgcccag aagctaggtg tagagcagcc tacattgtat
     4621 tggcatgtaa aaaataagcg ggctttgctc gacgccttag ccattgagat gttagatagg
     4681 caccatactc acttttgccc tttagaaggg gaaagctggc aagatttttt acgtaataac
     4741 gctaaaagtt ttagatgtgc tttactaagt catcgcgatg gagcaaaagt acatttaggt
     4801 acacggccta cagaaaaaca gtatgaaact ctcgaaaatc aattagcctt tttatgccaa
     4861 caaggttttt cactagagaa tgcattatat gcactcagcg ctgtggggca ttttacttta
     4921 ggttgcgtat tggaagatca agagcatcaa gtcgctaaag aagaaaggga aacacctact
     4981 actgatagta tgccgccatt attacgacaa gctatcgaat tatttgatca ccaaggtgca
     5041 gagccagcct tcttattcgg ccttgaattg atcatatgcg gattagaaaa acaacttaaa
     5101 tgtgaaagtg ggtcctaata acactgatag tgctagtgta gatcactact agagccaggc
     5161 atcaaataaa acgaaaggct cagtcgaaag actgggcctt tcgttttatc tgttgtttgt
     5221 cggtgaacgc tctctactag agtcacactg gctcaccttc gggtgggcct ttctgcgttt
     5281 atatactaga gtccctatca gtgatagaga ttgacatccc tatcagtgat agagatactg
     5341 agcactacta gagaagcttg cggaggagca tgcatggctt ccaaggtgta cgaccccgag
     5401 caacgcaaac gcatgatcac tgggcctcag tggtgggctc gctgcaagca aatgaacgtg
     5461 ctggactcct tcatcaacta ctatgattcc gagaagcacg ccgagaacgc cgtgattttt
     5521 ctgcatggta acgctgcctc cagctacctg tggaggcacg tcgtgcctca catcgagccc
     5581 gtggctagat gcatcatccc tgatctgatc ggaatgggta agtccggcaa gagcgggaat
     5641 ggctcatatc gcctcctgga tcactacaag tacctcaccg cttggttcga gctgctgaac
     5701 cttccaaaga aaatcatctt tgtgggccac gactgggggg cttgtctggc ctttcactac
     5761 tcctacgagc accaagacaa gatcaaggcc atcgtccatg ctgagagtgt cgtggacgtg
     5821 atcgagtcct gggacgagtg gcctgacatc gaggaggata tcgccctgat caagagcgaa
     5881 gagggcgaga aaatggtgct tgagaataac ttcttcgtcg agaccatgct cccaagcaag
     5941 atcatgcgga aactggagcc tgaggagttc gctgcctacc tggagccatt caaggagaag
     6001 ggcgaggtta gacggcctac cctctcctgg cctcgcgaga tccctctcgt taagggaggc
     6061 aagcccgacg tcgtccagat tgtccgcaac tacaacgcct accttcgggc cagcgacgat
     6121 ctgcctaaga tgttcatcga gtccgaccct gggttctttt ccaacgctat tgtcgaggga
     6181 gctaagaagt tccctaacac cgagttcgtg aaggtgaagg gcctccactt cagccaggag
     6241 gacgctccag atgaaatggg taagtacatc aagagcttcg tggagcgcgt gctgaagaac
     6301 gagcagtaat gagatcggat ctccaggcat caaataaaac gaaaggctca gtcgaaagac
     6361 tgggcctttc gttttatctg ttgtttgtcg gtgaacgctc tcctgagtag gacaaatccg
     6421 gatcccatgc aaggagatgg cgcccaacag tcccccggcc acggggcctg ccaccatacc
     6481 cacgccgaaa caagcgctca tgagcccgaa gtggcgagcc cgatcttccc catcggtgat
     6541 gtcggcgata taggcgccag caaccgcacc tgtggcgccg gtgatgccgg ccacgatgcg
     6601 tccggcgtag aggatcgaga tcgatctcga tcccgcgaaa ttaatacgac tcactatagg
     6661 ggaattgtga gcggataaca attcccctct agaaataatt ttgtttaact ttaagaagga
     6721 gatatacata tgttcactat agacgagatg ctatcaaaga acaaccaaag attggctttc
     6781 gagcacttcg ctactaagaa cgacggatgt ggccctgatg gtatgcacgt atctgagctg
     6841 gagaaatatt ggcgtatgaa tcacgatcag atcatctccg acttgaagaa ccaagagtac
     6901 caacccggca ttattttgat tagagaacac atgaacaaaa caggtaagag aaggaacatt
     6961 gcatctctca acgtgatcga caggttcata actcgactgc tgtcgcaaaa gttgaataga
     7021 tacttagccc ccattttctg cgagaactca tacgcttacc aagactcaaa aggggtgatg
     7081 cccgccgtac taaaagccaa ggagtacgtc gagttgggta tgagacacgt tatcgagata
     7141 gacctcaaga attacttcga cacgatcccg ttagagaact taatcccaga gatcgagcgt
     7201 tacataacag atgaggcagt gctgcacttg attaaacagt acttattctg tgacatctcg
     7261 ttcgagggta agatatcacg taaaactcaa gggatcgttc agggtaacgc aatttcccca
     7321 atactatcca acctctacct gaacgacttc gacaaggagc tggacgagtc gaagctgtgc
     7381 tggatcagat acgccgacaa catatacatc tatatggaca gctacgagaa ggccctactg
     7441 gtttattccg agcttaccga gagacttgag agaaggaaat taaccgttaa caaggagaaa
     7501 tcaggggtgt ttgatgttag tacccgttct atactaggtt acgacatcct gatcagaaac
     7561 aagaaggttg acgtgcgtaa acacatctac aagtctgtta accagtactc caactggcac
     7621 gactctcgat tggagttcat caacggtcgt taccacatca cttccgacgg tatcctcaat
     7681 agacaagact tcggtctact gttcgagaat gagcagaaga agcactacat acctgttgag
     7741 gtttctgacc agttgaatat ttacggaaac gttacgctcg ccagcaacgt tcttcaatca
     7801 ttctccaacc gagagatcaa agtgtccttc ttcgacaagt acgggagact tattggatct
     7861 ttcctgcccg agaaaactaa gaaaagcgcc gagataatac tggttcagtc taagaactac
     7921 cttaacgagg acgttaggat ggacacggcc cgacgtatgg agatcgccgg tttgcataac
     7981 atcagagcca acctcagata ctacgacaag aagcacaaag gtgactttaa ggaaaaggta
     8041 gacgccattt ctggctatat tgacgccctc aatagagcac catccgtgaa cgacatgatg
     8101 ctcttagagg ctaaagctcg tcagctgtac tacacatgct tcaaccagat cttagagaca
     8161 tccgactttc agttcgagaa gcgtaccaag cgaccaccaa aagacgccat caacgcctgt
     8221 atttcattcg gcaacacatt actgtacaac ttgtttgtga atatcatttg gaagaaggga
     8281 cttgatccaa ggttcggggt tgttcacgcc agcaacaagc gtaatcagtc ccttaacctg
     8341 gattttgctg acatcttcaa gcctatagtc atagatagga tcatcttcac aatgatcaac
     8401 aagaagatgc tgaccttgtt gacggacttc gagacttcta accagggagt ttacctttcc
     8461 cgagagggga agaacatctt cttgcagatg tacgaagaaa agctcaaatc cagaatcact
     8521 atcaagggaa aggagatgag ttactatcag ttgctagaat cagaggtgca gaactacaag
     8581 aacttcatcc tgaccggcga gacatacaag ccgtacaagt actactagcg tacgttaact
     8641 ttaagaagga gaacttaaga tgtatgtcat tctagtttac gacattcacc agaagcgagt
     8701 aggtaaggct ctaaagattt gtaggaagta cctgatccac atccagaagt cagttttcga
     8761 aggcaacata acggagagta agctaaaggc cttaaaagag gagctggggc acctgatcga
     8821 cactcagatg gactcggtaa ttatctacca cctggactcc gtgaagtaca ctaagaagga
     8881 gcagatcggc atcgtccaat cgacatccaa tgtcatttga ctcgagatcc ggctgctaac
     8941 aaagcccgaa aggaagctga gttggctgct gccaccgctg agcaataact agcataaccc
     9001 cttggggcct ctaaacgggt cttgaggggt tttttgctga aaggaggaac tatatccgga
     9061 taccaggtgc ggggaagctt atgttccata gcaaaaagtc ggtcagtctc gtggctgaaa
     9121 tcatgagttc cacaaaatgg ctgaaattca aggaaaatca ggaatctcag aaaaacgatc
     9181 gaccgacttt ttcgataaaa tggttgcaaa aatgagaaaa atctgattta atagaatctg
     9241 aaaacagcgg aaatgctgtt gtcgtacttt acctaaaagg aattgaaacg tcccgc
//
