LOCUS       pFS_0345_pET30-R        7656 bp    DNA     circular UNA 25-AUG-2018
DEFINITION  Concatenation of 2 sequences.
ACCESSION   urn.local...22-9fa11gb
VERSION     urn.local...22-9fa11gb
KEYWORDS    .
SOURCE      
  ORGANISM  
            .
FEATURES             Location/Qualifiers
     promoter        180..208
                     /Imported_from="<a
                     href=""http://wishart.biology.ualberta.ca/PlasMapper"">Pla
                     sMapper</a>"
                     /Transferred_From="amp prom"
                     /Transferred_Similarity="89.66%"
                     /label="amp prom"
     marker          complement(211..1026)
                     /Imported_from="<a
                     href=""http://wishart.biology.ualberta.ca/PlasMapper"">Pla
                     sMapper</a>"
                     /Transferred_From="kan2 marker"
                     /Transferred_Similarity="99.75%"
                     /label="kan2 marker"
     misc_feature    complement(212..1026)
                     /note="KanR"
                     /label="KanR"
     misc_feature    1132..1751
                     /Imported_from="<a
                     href=""http://wishart.biology.ualberta.ca/PlasMapper"">Pla
                     sMapper</a>"
                     /Transferred_From="pBR322 origin"
                     /Transferred_Similarity="99.68%"
                     /label="pBR322 origin"
                     /note="Geneious type: origin of replication"
     gene            complement(2163..2354)
                     /Imported_from="<a
                     href=""http://wishart.biology.ualberta.ca/PlasMapper"">Pla
                     sMapper</a>"
                     /Transferred_From="ROP"
                     /Transferred_Similarity="100.00%"
                     /label="ROP"
     misc_feature    complement(3163..4254)
                     /Imported_from="<a
                     href=""http://wishart.biology.ualberta.ca/PlasMapper"">Pla
                     sMapper</a>"
                     /Transferred_From="lacI reg"
                     /Transferred_Similarity="99.91%"
                     /label="lacI reg"
                     /note="Geneious type: regulatory sequence"
     promoter        4636..4654
                     /Imported_from="<a
                     href=""http://wishart.biology.ualberta.ca/PlasMapper"">Pla
                     sMapper</a>"
                     /Transferred_From="T7 prom"
                     /Transferred_Similarity="100.00%"
                     /label="T7 prom"
     misc_feature    4654..4681
                     /Imported_from="<a
                     href=""http://wishart.biology.ualberta.ca/PlasMapper"">Pla
                     sMapper</a>"
                     /Transferred_From="lacO reg"
                     /Transferred_Similarity="100.00%"
                     /label="lacO reg"
                     /note="Geneious type: regulatory sequence"
     gene            4699..4715
                     /Imported_from="<a
                     href=""http://wishart.biology.ualberta.ca/PlasMapper"">Pla
                     sMapper</a>"
                     /Transferred_From="T7_transl_en_RBS"
                     /Transferred_Similarity="100.00%"
                     /label="T7_transl_en_RBS"
     CDS             4724..6994
                     /created_by="User"
                     /label="Cas1"
     CDS             7026..7316
                     /created_by="User"
                     /label="Cas2"
     misc_feature    <7318..>7321
                     /note="XhoI"
                     /label="XhoI"
     terminator      7329..7457
                     /created_by="User"
                     /label="Terminator extended"
     terminator      7390..7433
                     /Imported_from="<a
                     href=""http://wishart.biology.ualberta.ca/PlasMapper"">Pla
                     sMapper</a>"
                     /Transferred_From="T7 term"
                     /Transferred_Similarity="97.73%"
                     /label="T7 term"
     misc_feature    complement(7458..>7463)
                     /label="CsiI"
     promoter        7465..7614
                     /created_by="User"
                     /label="Leader"
     repeat_region   7615..7649
                     /created_by="User"
                     /label="DR"
     misc_feature    7650..7654
                     /created_by="User"
                     /label="FaqI"
ORIGIN      
        1 ggccgctatt cttttgattt ataagggatt ttgccgattt cggcctattg gttaaaaaat
       61 gagctgattt aacaaaaatt taacgcgaat tttaacaaaa tattaacgct tacaatttca
      121 ggtggcactt ttcggggaaa tgtgcgcgga acccctattt gtttattttt ctaaatacat
      181 tcaaatatgt atccgctcat gaattaattc ttagaaaaac tcatcgagca tcaaatgaaa
      241 ctgcaattta ttcatatcag gattatcaat accatatttt tgaaaaagcc gtttctgtaa
      301 tgaaggagaa aactcaccga ggcagttcca taggatggca agatcctggt atcggtctgc
      361 gattccgact cgtccaacat caatacaacc tattaatttc ccctcgtcaa aaataaggtt
      421 atcaagtgag aaatcaccat gagtgacgac tgaatccggt gagaatggca aaagtttatg
      481 catttctttc cagacttgtt caacaggcca gccattacgc tcgtcatcaa aatcactcgc
      541 atcaaccaaa ccgttattca ttcgtgattg cgcctgagcg agacgaaata cgcgatcgct
      601 gttaaaagga caattacaaa caggaatcga atgcaaccgg cgcaggaaca ctgccagcgc
      661 atcaacaata ttttcacctg aatcaggata ttcttctaat acctggaatg ctgttttccc
      721 ggggatcgca gtggtgagta accatgcatc atcaggagta cggataaaat gcttgatggt
      781 cggaagaggc ataaattccg tcagccagtt tagtctgacc atctcatctg taacatcatt
      841 ggcaacgcta cctttgccat gtttcagaaa caactctggc gcatcgggct tcccatacaa
      901 tcgatagatt gtcgcacctg attgcccgac attatcgcga gcccatttat acccatataa
      961 atcagcatcc atgttggaat ttaatcgcgg cctagagcaa gacgtttccc gttgaatatg
     1021 gctcataaca ccccttgtat tactgtttat gtaagcagac agttttattg ttcatgacca
     1081 aaatccctta acgtgagttt tcgttccact gagcgtcaga ccccgtagaa aagatcaaag
     1141 gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac
     1201 cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa
     1261 ctggcttcag cagagcgcag ataccaaata ctgtccttct agtgtagccg tagttaggcc
     1321 accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag
     1381 tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac
     1441 cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc
     1501 gaacgaccta caccgaactg agatacctac agcgtgagct atgagaaagc gccacgcttc
     1561 ccgaagggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca
     1621 cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc
     1681 tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg
     1741 ccagcaacgc ggccttttta cggttcctgg tcttttgctg gccttttgct cacatgttct
     1801 ttcctgcgtt atcccctgat tctgtggata accgtattac cgcctttgag tgagctgata
     1861 ccgctcgccg cagccgaacg accgagcgca gcgagtcagt gagcgaggaa gcggaagagc
     1921 gcctgatgcg gtattttctc cttacgcatc tgtgcggtat ttcacaccgc aatggtgcac
     1981 tctcagtaca atctgctctg atgccgcata gttaagccag tatacactcc gctatcgcta
     2041 cgtgactggg tcatggctgc gccccgacac ccgccaacac ccgctgacgc gccctgacgg
     2101 gcttgtctgc tcccggcatc cgcttacaga caagctgtga ccgtctccgg gagctgcatg
     2161 tgtcagaggt tttcaccgtc atcaccgaaa cgcgcgaggc agctgcggta aagctcatca
     2221 gcgtggtcgt gaagcgattc acagatgtct gcctgttcat ccgcgtccag ctcgttgagt
     2281 ttctccagaa gcgttaatgt ctggcttctg ataaagcggg ccatgttaag ggcggttttt
     2341 tcctgtttgg tcactgatgc ctccgtgtaa gggggatttc tgttcatggg ggtaatgata
     2401 ccgatgaaac gagagaggat gctcacgata cgggttactg atgatgaaca tgcccggtta
     2461 ctggaacgtt gtgagggtaa acaactggcg gtatggatgc ggcgggacca gagaaaaatc
     2521 actcagggtc aatgccagcg cttcgttaat acagatgtag gtgttccaca gggtagccag
     2581 cagcatcctg cgatgcagat ccggaacata atggtgcagg gcgctgactt ccgcgtttcc
     2641 agactttacg aaacacggaa accgaagatc attcatgttg ttgctcaggt cgcagacgtt
     2701 ttgcagcagc agtcgcttca cgttcgctcg cgtatcggtg attcattctg ctaaccagta
     2761 aggcaacccc gccagcctag ccgggtcctc aacgacagga gcacgatcat gcgcacccgt
     2821 ggggccgcca tgccggcgat aatggcctgc ttctcgccga aacgtttggt ggcgggacca
     2881 gtgacgaagg cttgagcgag ggcgtgcaag attccgaata ccgcaagcga caggccgatc
     2941 atcgtcgcgc tccagcgaaa gcggtcctcg ccgaaaatga cccagagcgc tgccggcacc
     3001 tgtcctacga gttgcatgat aaagaagata gtcataagtg cggcgacgat agtcatgccc
     3061 cgcgcccacc ggaaggagct gactgggttg aaggctctca agggcatcgg tcgagatccc
     3121 ggtgcctaat gagtgagcta acttacatta attgcgttgc gctcactgcc cgctttccag
     3181 tcgggaaacc tgtcgtgcca gctgcattaa tgaatcggcc aacgcgcggg gagaggcggt
     3241 ttgcgtattg ggcgccaggg tggtttttct tttcaccagt gagacgggca acagctgatt
     3301 gcccttcacc gcctggccct gagagagttg cagcaagcgg tccacgctgg tttgccccag
     3361 caggcgaaaa tcctgtttga tggtggttaa cggcgggata taacatgagc tatcttcggt
     3421 atcgtcgtat cccactaccg agatgtccgc accaacgcgc agcccggact cggtaatggc
     3481 gcgcattgcg cccagcgcca tctgatcgtt ggcaaccagc atcgcagtgg gaacgatgcc
     3541 ctcattcagc atttgcatgg tttgttgaaa accggacatg gcactccagt cgccttcccg
     3601 ttccgctatc ggctgaattt gattgcgagt gagatattta tgccagccag ccagacgcag
     3661 acgcgccgag acagaactta atgggcccgc taacagcgcg atttgctggt gacccaatgc
     3721 gaccagatgc tccacgccca gtcgcgtacc atcttcatgg gagaaaataa tactgttgat
     3781 gggtgtctgg tcagagacat caagaaataa cgccggaaca ttagtgcagg cagcttccac
     3841 agcaatggca tcctggtcat ccagcggata gttaatgatc agcccactga cgcgttgcgc
     3901 gagaagattg tgcaccgccg ctttacaggc ttcgacgccg cttcgttcta ccatcgacac
     3961 caccacgctg gcacccagtt gatcggcgcg agatttaatc gccgcgacaa tttgcgacgg
     4021 cgcgtgcagg gccagactgg aggtggcaac gccaatcagc aacgactgtt tgcccgccag
     4081 ttgttgtgcc acgcggttgg gaatgtaatt cagctccgcc atcgccgctt ccactttttc
     4141 ccgcgttttc gcagaaacgt ggctggcctg gttcaccacg cgggaaacgg tctgataaga
     4201 gacaccggca tactctgcga catcgtataa cgttactggt ttcacattca ccaccctgaa
     4261 ttgactctct tccgggcgct atcatgccat accgcgaaag gttttgcgcc attcgatggt
     4321 gtccgggatc tcgacgctct cccttatgcg actcctgcat taggaagcag cccagtagta
     4381 ggttgaggcc gttgagcacc gccgccgcaa ggaatggtgc atgcaaggag atggcgccca
     4441 acagtccccc ggccacgggg cctgccacca tacccacgcc gaaacaagcg ctcatgagcc
     4501 cgaagtggcg agcccgatct tccccatcgg tgatgtcggc gatataggcg ccagcaaccg
     4561 cacctgtggc gccggtgatg ccggccacga tgcgtccggc gtagaggatc gagatcgatc
     4621 tcgatcccgc gaaattaata cgactcacta taggggaatt gtgagcggat aacaattccc
     4681 ctctagaaat aattttgttt aactttaaga aggagatata catatgccag actactacca
     4741 ctccatcact actctccatg cacttcagaa cgcttggaga gcagttagag ctaagaacgc
     4801 tgccggtggt atagacggtt ttacgctttc ccacttcgag aaaagactta atgacaactt
     4861 aatcgagttg cagcacgagt tgatctcaca gacttggaac cctgagccct atctgagaat
     4921 cgagataacg aagaacgaaa cggagaagcg taagctaggt ttgctctgta tcaaagataa
     4981 gattgtccag caggctatca agaccgctat cgagccacaa ctggaaaaga ctttccttaa
     5041 cctgtcctat ggatatagac ctaataaggg cccagagaga gccattaaga gagttgtgca
     5101 tgacttgaag aagctgaaat ctggatacgt cgctaagttg gacatcgata attacttcga
     5161 cacaataaac cacgagagac tatttaccag actagcaaac tggctaaagg acgacgagac
     5221 tttgagatta attaggttgt gcattcagac cggcattgtc acgcctcagc tacagtggca
     5281 ggagattaac aagggcgtcc cccaaggggc cattctctca ccgttgctgg ccaatttcta
     5341 cttacacccg ttcgaccaat tcgcagctaa caaggttcca atgtacatca gatatgccga
     5401 tgacttcctg atagccactt cgactgagaa gcagatcaag gaagccgtgg agctagtcaa
     5461 agaggagctt gagtcacagt tctacttgca gttaaacact cccatcattc acaactttca
     5521 cgacggtatt gagttcctgg gtattaccat tagcgacact ggactgtcca ttaccgagaa
     5581 gaagaagaaa accttgcagg aaaggattaa ctccattaag ttcatcaagt catccctgag
     5641 ttcgcagtct aaggaaacat tacagggcat taagaactat tacgctaaat tattgcctga
     5701 gtctaccctg aaagagctag actgttttct gatgaatcgt ctgaacgctc tcatcataag
     5761 aaatcagaat agtatcaaca ataagaagga gctagtctct aacttgcaga agatcgagtt
     5821 ttactctgag aactcaaaca agaacaagag ccagctcatc cagcagttgt gctcaactta
     5881 cattgtccac agtactaagt ccaaaacgag attgacttcc actcacatcg acaacaccaa
     5941 attaattact cagaagaaga aggagtacca aaagagagag aacgagggag cagagcttgt
     6001 tattagcatt cccggaagtt acatcggcgc tacatacaag ggtataactg ttaagctaca
     6061 aggcaaaatc ataaacaagc cctcgccggc cttgaagcat atcactgtcg ttggaaaagg
     6121 tatctctctt tcttcaaacg ctataaccta ctgtatgaat cataagatcc ctatcgattt
     6181 ctttgacgga aggggtaagc agtacggaac agtcttaaac cccgttttcc ttgacggcac
     6241 gctgtggaac aagcaagtcg agcttccctt agagcagaag attaagctgg ccacccagat
     6301 aatcatcgga aagctgaaga accagcttaa cttgatcaaa tactatcaca agtatcacaa
     6361 ggacattcta ggcggcaaac tttccgagaa gtacgtagag gtggtgttga agattgataa
     6421 attgatcgaa aaggcaaaga actactcgca acgaaacgag aagtacaccg ctgagttgat
     6481 ggctatcgaa tcgcaagccg ccattgctta ctggtcctat attagagtcc taaccgccga
     6541 cgatggcatc gacttcatta ggcgagaaca tcagggagct actgacctgt tgaatagttt
     6601 gctgaattac ggttacgcca tcttgtacgc cagggtgtgg aagaacatct tggcagcaaa
     6661 gctcaacccc tcaatcggtg ttctgcacgc caaacaggac ggaaagccaa ccttggtgtt
     6721 cgacgtggtg gaactgttca gggcacagat ggtcgatcgt gtcgttatct ctttaatcca
     6781 gaagaaggtg agcttgaaga tgcacgatgg attattgaat gagtccagta agagagtgct
     6841 tataaggtac atcttggaaa gattaaacag gtacgagaag tacaggggcg aggagattac
     6901 ttttagtcag atcatcctca ggcaggcaca ggagattgct ttgttcatat cgggcgataa
     6961 ccttatcttc aagccatacg tagctaagtg gtgacgtacg ttaactttaa gaaggagaac
     7021 ttaagatggt taaggccaag aagatcttct gcgtcgtcgc ttatgacatc caggacgaca
     7081 ggtctaggat tcagatcagc aagatccttg agaagtacgg gaccagaata aactactccg
     7141 tctttgagtg catgttcacc gaccgtcagt tccagaaaat acagataaac ctggagcgat
     7201 ggataaacag acgttacgac acggttgtct actaccctat gtgtattaac tgttacaccc
     7261 gtatcatcta ccagccaatt aggaagaaga ttatcaagac tgtggagatc gtgtgactcg
     7321 agatccggct gctaacaaag cccgaaagga agctgagttg gctgctgcca ccgctgagca
     7381 ataactagca taaccccttg gggcctctaa acgggtcttg aggggttttt tgctgaaagg
     7441 aggaactata tccggatacc aggttatatt ctacaaatta tttatttaaa ttacagaaaa
     7501 aaagagtaca tttgcgaaaa tcagttcaac acttcatcta tctaactgaa taataagtta
     7561 ttatatattt atacagatag aatacatatt ttataataat ctgattatca atatatgtag
     7621 atgtattcca gtataataag gattaagacg tcccgc
//
