LOCUS       pFS_0350_pET30-R        7228 bp    DNA     circular UNA 25-AUG-2018
DEFINITION  Concatenation of 3 sequences.
ACCESSION   urn.local...27-9fa11gc
VERSION     urn.local...27-9fa11gc
KEYWORDS    .
SOURCE      
  ORGANISM  
            .
FEATURES             Location/Qualifiers
     promoter        7..>126
                     /created_by="User"
                     /label="Leader"
     promoter        <123..156
                     /created_by="User"
                     /label="Leader"
     repeat_region   157..192
                     /created_by="User"
                     /label="DR"
     misc_feature    193..197
                     /created_by="User"
                     /label="FaqI"
     promoter        379..407
                     /Imported_from="<a
                     href=""http://wishart.biology.ualberta.ca/PlasMapper"">Pla
                     sMapper</a>"
                     /Transferred_From="amp prom"
                     /Transferred_Similarity="89.66%"
                     /label="amp prom"
     marker          complement(410..1225)
                     /Imported_from="<a
                     href=""http://wishart.biology.ualberta.ca/PlasMapper"">Pla
                     sMapper</a>"
                     /Transferred_From="kan2 marker"
                     /Transferred_Similarity="99.75%"
                     /label="kan2 marker"
     misc_feature    complement(411..1225)
                     /note="KanR"
                     /label="KanR"
     misc_feature    1331..1950
                     /Imported_from="<a
                     href=""http://wishart.biology.ualberta.ca/PlasMapper"">Pla
                     sMapper</a>"
                     /Transferred_From="pBR322 origin"
                     /Transferred_Similarity="99.68%"
                     /label="pBR322 origin"
                     /note="Geneious type: origin of replication"
     gene            complement(2362..2553)
                     /Imported_from="<a
                     href=""http://wishart.biology.ualberta.ca/PlasMapper"">Pla
                     sMapper</a>"
                     /Transferred_From="ROP"
                     /Transferred_Similarity="100.00%"
                     /label="ROP"
     misc_feature    complement(3362..4453)
                     /Imported_from="<a
                     href=""http://wishart.biology.ualberta.ca/PlasMapper"">Pla
                     sMapper</a>"
                     /Transferred_From="lacI reg"
                     /Transferred_Similarity="99.91%"
                     /label="lacI reg"
                     /note="Geneious type: regulatory sequence"
     promoter        4835..4853
                     /Imported_from="<a
                     href=""http://wishart.biology.ualberta.ca/PlasMapper"">Pla
                     sMapper</a>"
                     /Transferred_From="T7 prom"
                     /Transferred_Similarity="100.00%"
                     /label="T7 prom"
     misc_feature    4853..4880
                     /Imported_from="<a
                     href=""http://wishart.biology.ualberta.ca/PlasMapper"">Pla
                     sMapper</a>"
                     /Transferred_From="lacO reg"
                     /Transferred_Similarity="100.00%"
                     /label="lacO reg"
                     /note="Geneious type: regulatory sequence"
     gene            4898..4914
                     /Imported_from="<a
                     href=""http://wishart.biology.ualberta.ca/PlasMapper"">Pla
                     sMapper</a>"
                     /Transferred_From="T7_transl_en_RBS"
                     /Transferred_Similarity="100.00%"
                     /label="T7_transl_en_RBS"
     misc_feature    4920..4925
                     /Recognition_pattern="CA^TATG"
                     /annotation_group="CATATG"
                     /label="NdeI"
                     /note="Geneious type: restriction site"
     CDS             4923..6776
                     /created_by="User"
                     /label="Cas1"
     gene            6783..6799
                     /Imported_from="<a
                     href=""http://wishart.biology.ualberta.ca/PlasMapper"">Pla
                     sMapper</a>"
                     /X-Transferred_From="T7_transl_en_RBS"
                     /X-Transferred_Similarity="100.00%"
                     /Transferred_From="Digestion of
                     pFSNW_013_pET30bvectorNovagen_Golden Gate_T7_Terminator -
                     Fragment 2, Ligated sequences"
                     /Transferred_Similarity="100.00%"
                     /label="T7_transl_en_RBS"
     misc_feature    6802..6807
                     /Recognition_pattern="C^TTAAG"
                     /annotation_group="CTTAAG"
                     /label="AflII"
                     /note="Geneious type: restriction site"
     CDS             6808..7086
                     /created_by="User"
                     /label="Cas2"
     misc_feature    <7088..>7091
                     /note="XhoI"
                     /label="XhoI"
     terminator      7099..7227
                     /created_by="User"
                     /label="Terminator extended"
     terminator      7160..7203
                     /Imported_from="<a
                     href=""http://wishart.biology.ualberta.ca/PlasMapper"">Pla
                     sMapper</a>"
                     /Transferred_From="T7 term"
                     /Transferred_Similarity="97.73%"
                     /label="T7 term"
     misc_feature    complement(7228..>5)
                     /label="CsiI"
ORIGIN      
        1 ccaggtcaca cggcacgcga cagcgactcg accatcgtga gagctcatca gaacgccccg
       61 ccgtgcacaa gggtgaaatt gataccgctc gctgacctga ggctggagcc gcccgaacgc
      121 gcggccgccc gcctctcgtt ggcgtagccg cccgccgttt caatgccctc agtttcgggc
      181 catgggtgga acgtcccgcg gccgctattc ttttgattta taagggattt tgccgatttc
      241 ggcctattgg ttaaaaaatg agctgattta acaaaaattt aacgcgaatt ttaacaaaat
      301 attaacgctt acaatttcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg
      361 tttatttttc taaatacatt caaatatgta tccgctcatg aattaattct tagaaaaact
      421 catcgagcat caaatgaaac tgcaatttat tcatatcagg attatcaata ccatattttt
      481 gaaaaagccg tttctgtaat gaaggagaaa actcaccgag gcagttccat aggatggcaa
      541 gatcctggta tcggtctgcg attccgactc gtccaacatc aatacaacct attaatttcc
      601 cctcgtcaaa aataaggtta tcaagtgaga aatcaccatg agtgacgact gaatccggtg
      661 agaatggcaa aagtttatgc atttctttcc agacttgttc aacaggccag ccattacgct
      721 cgtcatcaaa atcactcgca tcaaccaaac cgttattcat tcgtgattgc gcctgagcga
      781 gacgaaatac gcgatcgctg ttaaaaggac aattacaaac aggaatcgaa tgcaaccggc
      841 gcaggaacac tgccagcgca tcaacaatat tttcacctga atcaggatat tcttctaata
      901 cctggaatgc tgttttcccg gggatcgcag tggtgagtaa ccatgcatca tcaggagtac
      961 ggataaaatg cttgatggtc ggaagaggca taaattccgt cagccagttt agtctgacca
     1021 tctcatctgt aacatcattg gcaacgctac ctttgccatg tttcagaaac aactctggcg
     1081 catcgggctt cccatacaat cgatagattg tcgcacctga ttgcccgaca ttatcgcgag
     1141 cccatttata cccatataaa tcagcatcca tgttggaatt taatcgcggc ctagagcaag
     1201 acgtttcccg ttgaatatgg ctcataacac cccttgtatt actgtttatg taagcagaca
     1261 gttttattgt tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac
     1321 cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc
     1381 ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca
     1441 actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgtccttcta
     1501 gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct
     1561 ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg
     1621 gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc
     1681 acacagccca gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta
     1741 tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg
     1801 gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt
     1861 cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg
     1921 cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggt cttttgctgg
     1981 ccttttgctc acatgttctt tcctgcgtta tcccctgatt ctgtggataa ccgtattacc
     2041 gcctttgagt gagctgatac cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg
     2101 agcgaggaag cggaagagcg cctgatgcgg tattttctcc ttacgcatct gtgcggtatt
     2161 tcacaccgca atggtgcact ctcagtacaa tctgctctga tgccgcatag ttaagccagt
     2221 atacactccg ctatcgctac gtgactgggt catggctgcg ccccgacacc cgccaacacc
     2281 cgctgacgcg ccctgacggg cttgtctgct cccggcatcc gcttacagac aagctgtgac
     2341 cgtctccggg agctgcatgt gtcagaggtt ttcaccgtca tcaccgaaac gcgcgaggca
     2401 gctgcggtaa agctcatcag cgtggtcgtg aagcgattca cagatgtctg cctgttcatc
     2461 cgcgtccagc tcgttgagtt tctccagaag cgttaatgtc tggcttctga taaagcgggc
     2521 catgttaagg gcggtttttt cctgtttggt cactgatgcc tccgtgtaag ggggatttct
     2581 gttcatgggg gtaatgatac cgatgaaacg agagaggatg ctcacgatac gggttactga
     2641 tgatgaacat gcccggttac tggaacgttg tgagggtaaa caactggcgg tatggatgcg
     2701 gcgggaccag agaaaaatca ctcagggtca atgccagcgc ttcgttaata cagatgtagg
     2761 tgttccacag ggtagccagc agcatcctgc gatgcagatc cggaacataa tggtgcaggg
     2821 cgctgacttc cgcgtttcca gactttacga aacacggaaa ccgaagatca ttcatgttgt
     2881 tgctcaggtc gcagacgttt tgcagcagca gtcgcttcac gttcgctcgc gtatcggtga
     2941 ttcattctgc taaccagtaa ggcaaccccg ccagcctagc cgggtcctca acgacaggag
     3001 cacgatcatg cgcacccgtg gggccgccat gccggcgata atggcctgct tctcgccgaa
     3061 acgtttggtg gcgggaccag tgacgaaggc ttgagcgagg gcgtgcaaga ttccgaatac
     3121 cgcaagcgac aggccgatca tcgtcgcgct ccagcgaaag cggtcctcgc cgaaaatgac
     3181 ccagagcgct gccggcacct gtcctacgag ttgcatgata aagaagatag tcataagtgc
     3241 ggcgacgata gtcatgcccc gcgcccaccg gaaggagctg actgggttga aggctctcaa
     3301 gggcatcggt cgagatcccg gtgcctaatg agtgagctaa cttacattaa ttgcgttgcg
     3361 ctcactgccc gctttccagt cgggaaacct gtcgtgccag ctgcattaat gaatcggcca
     3421 acgcgcgggg agaggcggtt tgcgtattgg gcgccagggt ggtttttctt ttcaccagtg
     3481 agacgggcaa cagctgattg cccttcaccg cctggccctg agagagttgc agcaagcggt
     3541 ccacgctggt ttgccccagc aggcgaaaat cctgtttgat ggtggttaac ggcgggatat
     3601 aacatgagct atcttcggta tcgtcgtatc ccactaccga gatgtccgca ccaacgcgca
     3661 gcccggactc ggtaatggcg cgcattgcgc ccagcgccat ctgatcgttg gcaaccagca
     3721 tcgcagtggg aacgatgccc tcattcagca tttgcatggt ttgttgaaaa ccggacatgg
     3781 cactccagtc gccttcccgt tccgctatcg gctgaatttg attgcgagtg agatatttat
     3841 gccagccagc cagacgcaga cgcgccgaga cagaacttaa tgggcccgct aacagcgcga
     3901 tttgctggtg acccaatgcg accagatgct ccacgcccag tcgcgtacca tcttcatggg
     3961 agaaaataat actgttgatg ggtgtctggt cagagacatc aagaaataac gccggaacat
     4021 tagtgcaggc agcttccaca gcaatggcat cctggtcatc cagcggatag ttaatgatca
     4081 gcccactgac gcgttgcgcg agaagattgt gcaccgccgc tttacaggct tcgacgccgc
     4141 ttcgttctac catcgacacc accacgctgg cacccagttg atcggcgcga gatttaatcg
     4201 ccgcgacaat ttgcgacggc gcgtgcaggg ccagactgga ggtggcaacg ccaatcagca
     4261 acgactgttt gcccgccagt tgttgtgcca cgcggttggg aatgtaattc agctccgcca
     4321 tcgccgcttc cactttttcc cgcgttttcg cagaaacgtg gctggcctgg ttcaccacgc
     4381 gggaaacggt ctgataagag acaccggcat actctgcgac atcgtataac gttactggtt
     4441 tcacattcac caccctgaat tgactctctt ccgggcgcta tcatgccata ccgcgaaagg
     4501 ttttgcgcca ttcgatggtg tccgggatct cgacgctctc ccttatgcga ctcctgcatt
     4561 aggaagcagc ccagtagtag gttgaggccg ttgagcaccg ccgccgcaag gaatggtgca
     4621 tgcaaggaga tggcgcccaa cagtcccccg gccacggggc ctgccaccat acccacgccg
     4681 aaacaagcgc tcatgagccc gaagtggcga gcccgatctt ccccatcggt gatgtcggcg
     4741 atataggcgc cagcaaccgc acctgtggcg ccggtgatgc cggccacgat gcgtccggcg
     4801 tagaggatcg agatcgatct cgatcccgcg aaattaatac gactcactat aggggaattg
     4861 tgagcggata acaattcccc tctagaaata attttgttta actttaagaa ggagatatac
     4921 atatggctag agtcgcagat gaaggagcct tgacgagagc ttggtccgaa gttcttgagg
     4981 ctgctgaaag agatggcaga gtaccagtga gcgtccgaag atttgagaga ggtgttgctg
     5041 ctagtctagt gcgactttct ggagaattgt catctggaag atatcagcca tcaagagttt
     5101 cagaagttag cctaagaact ggtagtggct cagaaagagt tttgagaatt ggcgcagttg
     5161 tagatcgtgt tgtagaaagg agtttgctta acgctttgac tcccgttatt gatcctctac
     5221 tgtccccttt tgcttttggt tttaggagag gtctaggcgt taaagatgcc gtagcagctt
     5281 tggctagagc tagagatgaa ggttctacac atgtgcttag aagtgatatt gccgccgctt
     5341 ttgattccgt gccaagggca cgagctgttc aggcactttc acgtttagta ccagatagac
     5401 gtgtttgtga tgtcgttgcc tctttactgg ctagactaga tgattatggt ttagaaggtg
     5461 tgggtattgc tcaaggttca gctgtatctc cactcctctt aaatttgtat ttacttccct
     5521 ttgatgaggc tttgatggct aatggtttta caccacttag atacgctgat gatattgctg
     5581 ttccagctat gtcggaaagt caagcccaaa gtgctgccca agatgtcgcc catcaattag
     5641 aatgtctagg tttggcttgt agtgcaccca aaactagcat caggtccttt gatgaaggtg
     5701 ttcatttctt aggggtaact ttgagagcta gaccttcttc gacagcacca cccccagctc
     5761 gatcaaggcc tcaaagaatt tctatggtcg tttctgaggg tggtgctgta gttagaacaa
     5821 gacgtggtcg agttagagta gaccgagatg gagaaactgt tgcctcaatg tcattgtcca
     5881 gagtagcacg tattgttgtt cagggtcgtg taggtttgac gactccactt cttcatgaag
     5941 ctgctcagag aggtattgat gttgtaatgc ttagtagatc gggtgggtat gttggtaggc
     6001 tatcaagaag gcgtccaggt gatccttctt tgaggagagc acaagccaga gcatacgatt
     6061 ctggagctga tttggagagg cttacaactg cttttgtttc cggtaagatt actaatatga
     6121 gagtagccgt attgcgacac caaagaggcg ccggaacttc cgaagaagga gccagagtcg
     6181 ctgcacaact tgctgaagcc cgagcaaggg catctgttat gaggtctgta ccttctttaa
     6241 tgggagtaga aggggccgca acaagagcct attttggttg gctaggctcg cgagttggcg
     6301 aagaatgggg atttcatgga cgtgctagaa ggccaccccc tgaccctgtt aatagtatgt
     6361 tatcctatgg atatgtatta ttgtgcgctg aaggagtttc tgcttgtgaa caagctggtc
     6421 ttgatcctga tatgggcttt ctacattctg atcgatgggg tcgtccgagt ttggcactag
     6481 atttgatgga agaatggcgt cctgtcattg ttgattcgac tgtgttgagg attatttcga
     6541 ataaaagact aaagccatca gactttacat ttgatgccaa acaaggagca aggatgaccg
     6601 ctcatgctag acaaactttt ctgagagaat atgaagcaag aatgttgacg cttgctggat
     6661 cagatgccgg ggctggtcgt caaccatata gaagattgat tgctactcaa gctatgagac
     6721 ttgcagaggc tttgagaaca cccggtggtc catatcgtcc ttatgtttgg agatagcgta
     6781 cgttaacttt aagaaggaga acttaagatg atatggatct tagcatgtta tgatatagct
     6841 gacgatgatc gaagatccag attatctgat cttctagcag agttaggccc aagagtccaa
     6901 cagagcgtct ttgaatgtag acttccctct aagaaggctt tgagaagatt attgggagaa
     6961 cttgctgcac ttatcgaccc cgttgaagat caggtgcgtg tttacgagct tggtggccaa
     7021 ggacccagac cgcaaattgt tggcactcgt atcctagaag aatggcgaga catgtgggtt
     7081 gtttaactcg agatccggct gctaacaaag cccgaaagga agctgagttg gctgctgcca
     7141 ccgctgagca ataactagca taaccccttg gggcctctaa acgggtcttg aggggttttt
     7201 tgctgaaagg aggaactata tccggata
//
