LOCUS       pFS_0351_pET30-R        7248 bp    DNA     circular UNA 25-AUG-2018
DEFINITION  Concatenation of 2 sequences.
ACCESSION   urn.local...28-9fa11gc
VERSION     urn.local...28-9fa11gc
KEYWORDS    .
SOURCE      
  ORGANISM  
            .
FEATURES             Location/Qualifiers
     promoter        7..156
                     /created_by="User"
                     /label="Leader"
     repeat_region   157..185
                     /created_by="User"
                     /label="DR"
     misc_feature    186..190
                     /created_by="User"
                     /label="FaqI"
     promoter        372..400
                     /Imported_from="<a
                     href=""http://wishart.biology.ualberta.ca/PlasMapper"">Pla
                     sMapper</a>"
                     /Transferred_From="amp prom"
                     /Transferred_Similarity="89.66%"
                     /label="amp prom"
     marker          complement(403..1218)
                     /Imported_from="<a
                     href=""http://wishart.biology.ualberta.ca/PlasMapper"">Pla
                     sMapper</a>"
                     /Transferred_From="kan2 marker"
                     /Transferred_Similarity="99.75%"
                     /label="kan2 marker"
     misc_feature    complement(404..1218)
                     /note="KanR"
                     /label="KanR"
     misc_feature    1324..1943
                     /Imported_from="<a
                     href=""http://wishart.biology.ualberta.ca/PlasMapper"">Pla
                     sMapper</a>"
                     /Transferred_From="pBR322 origin"
                     /Transferred_Similarity="99.68%"
                     /label="pBR322 origin"
                     /note="Geneious type: origin of replication"
     gene            complement(2355..2546)
                     /Imported_from="<a
                     href=""http://wishart.biology.ualberta.ca/PlasMapper"">Pla
                     sMapper</a>"
                     /Transferred_From="ROP"
                     /Transferred_Similarity="100.00%"
                     /label="ROP"
     misc_feature    complement(3355..4446)
                     /Imported_from="<a
                     href=""http://wishart.biology.ualberta.ca/PlasMapper"">Pla
                     sMapper</a>"
                     /Transferred_From="lacI reg"
                     /Transferred_Similarity="99.91%"
                     /label="lacI reg"
                     /note="Geneious type: regulatory sequence"
     promoter        4828..4846
                     /Imported_from="<a
                     href=""http://wishart.biology.ualberta.ca/PlasMapper"">Pla
                     sMapper</a>"
                     /Transferred_From="T7 prom"
                     /Transferred_Similarity="100.00%"
                     /label="T7 prom"
     misc_feature    4846..4873
                     /Imported_from="<a
                     href=""http://wishart.biology.ualberta.ca/PlasMapper"">Pla
                     sMapper</a>"
                     /Transferred_From="lacO reg"
                     /Transferred_Similarity="100.00%"
                     /label="lacO reg"
                     /note="Geneious type: regulatory sequence"
     gene            4891..4907
                     /Imported_from="<a
                     href=""http://wishart.biology.ualberta.ca/PlasMapper"">Pla
                     sMapper</a>"
                     /Transferred_From="T7_transl_en_RBS"
                     /Transferred_Similarity="100.00%"
                     /label="T7_transl_en_RBS"
     CDS             4916..6814
                     /created_by="User"
                     /label="Cas1"
     gene            6821..6837
                     /Imported_from="<a
                     href=""http://wishart.biology.ualberta.ca/PlasMapper"">Pla
                     sMapper</a>"
                     /X-Transferred_From="Digestion of
                     pFSNW_013_pET30bvectorNovagen_Golden Gate_T7_Terminator -
                     Fragment 2; Ligated sequences; Ligated sequences 2;
                     T7_transl_en_RBS"
                     /X-Transferred_Similarity="100.00%"
                     /Transferred_From="Digestion of
                     pFSNW_013_pET30bvectorNovagen_Golden Gate_T7_Terminator -
                     Fragment 2, Ligated sequences, Ligated sequences 2"
                     /Transferred_Similarity="100.00%"
                     /label="T7_transl_en_RBS"
     CDS             6846..7106
                     /created_by="User"
                     /label="Cas2"
     misc_feature    <7108..>7111
                     /note="XhoI"
                     /label="XhoI"
     terminator      7119..7247
                     /created_by="User"
                     /label="Terminator extended"
     terminator      7180..7223
                     /Imported_from="<a
                     href=""http://wishart.biology.ualberta.ca/PlasMapper"">Pla
                     sMapper</a>"
                     /Transferred_From="T7 term"
                     /Transferred_Similarity="97.73%"
                     /label="T7 term"
     misc_feature    complement(7248..>5)
                     /label="CsiI"
ORIGIN      
        1 ccaggtatgc ctgaaatcat gaattccgca aaatggcgga aatttaagga aaatcaggaa
       61 tctcagaaaa acgatcgacc gacttttgtg ataaaatggt tgcaaaaaag agaaaaattt
      121 gatttaatag aatgtgaaaa tagcggaaat gctgatgttg taccttacct atgaggaatt
      181 gaaacgtccc gcggccgcta ttcttttgat ttataaggga ttttgccgat ttcggcctat
      241 tggttaaaaa atgagctgat ttaacaaaaa tttaacgcga attttaacaa aatattaacg
      301 cttacaattt caggtggcac ttttcgggga aatgtgcgcg gaacccctat ttgtttattt
      361 ttctaaatac attcaaatat gtatccgctc atgaattaat tcttagaaaa actcatcgag
      421 catcaaatga aactgcaatt tattcatatc aggattatca ataccatatt tttgaaaaag
      481 ccgtttctgt aatgaaggag aaaactcacc gaggcagttc cataggatgg caagatcctg
      541 gtatcggtct gcgattccga ctcgtccaac atcaatacaa cctattaatt tcccctcgtc
      601 aaaaataagg ttatcaagtg agaaatcacc atgagtgacg actgaatccg gtgagaatgg
      661 caaaagttta tgcatttctt tccagacttg ttcaacaggc cagccattac gctcgtcatc
      721 aaaatcactc gcatcaacca aaccgttatt cattcgtgat tgcgcctgag cgagacgaaa
      781 tacgcgatcg ctgttaaaag gacaattaca aacaggaatc gaatgcaacc ggcgcaggaa
      841 cactgccagc gcatcaacaa tattttcacc tgaatcagga tattcttcta atacctggaa
      901 tgctgttttc ccggggatcg cagtggtgag taaccatgca tcatcaggag tacggataaa
      961 atgcttgatg gtcggaagag gcataaattc cgtcagccag tttagtctga ccatctcatc
     1021 tgtaacatca ttggcaacgc tacctttgcc atgtttcaga aacaactctg gcgcatcggg
     1081 cttcccatac aatcgataga ttgtcgcacc tgattgcccg acattatcgc gagcccattt
     1141 atacccatat aaatcagcat ccatgttgga atttaatcgc ggcctagagc aagacgtttc
     1201 ccgttgaata tggctcataa caccccttgt attactgttt atgtaagcag acagttttat
     1261 tgttcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag
     1321 aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa
     1381 caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt
     1441 ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc
     1501 cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa
     1561 tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa
     1621 gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc
     1681 ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa
     1741 gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa
     1801 caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg
     1861 ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc
     1921 tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggtcttttgc tggccttttg
     1981 ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg
     2041 agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg
     2101 aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc
     2161 gcaatggtgc actctcagta caatctgctc tgatgccgca tagttaagcc agtatacact
     2221 ccgctatcgc tacgtgactg ggtcatggct gcgccccgac acccgccaac acccgctgac
     2281 gcgccctgac gggcttgtct gctcccggca tccgcttaca gacaagctgt gaccgtctcc
     2341 gggagctgca tgtgtcagag gttttcaccg tcatcaccga aacgcgcgag gcagctgcgg
     2401 taaagctcat cagcgtggtc gtgaagcgat tcacagatgt ctgcctgttc atccgcgtcc
     2461 agctcgttga gtttctccag aagcgttaat gtctggcttc tgataaagcg ggccatgtta
     2521 agggcggttt tttcctgttt ggtcactgat gcctccgtgt aagggggatt tctgttcatg
     2581 ggggtaatga taccgatgaa acgagagagg atgctcacga tacgggttac tgatgatgaa
     2641 catgcccggt tactggaacg ttgtgagggt aaacaactgg cggtatggat gcggcgggac
     2701 cagagaaaaa tcactcaggg tcaatgccag cgcttcgtta atacagatgt aggtgttcca
     2761 cagggtagcc agcagcatcc tgcgatgcag atccggaaca taatggtgca gggcgctgac
     2821 ttccgcgttt ccagacttta cgaaacacgg aaaccgaaga tcattcatgt tgttgctcag
     2881 gtcgcagacg ttttgcagca gcagtcgctt cacgttcgct cgcgtatcgg tgattcattc
     2941 tgctaaccag taaggcaacc ccgccagcct agccgggtcc tcaacgacag gagcacgatc
     3001 atgcgcaccc gtggggccgc catgccggcg ataatggcct gcttctcgcc gaaacgtttg
     3061 gtggcgggac cagtgacgaa ggcttgagcg agggcgtgca agattccgaa taccgcaagc
     3121 gacaggccga tcatcgtcgc gctccagcga aagcggtcct cgccgaaaat gacccagagc
     3181 gctgccggca cctgtcctac gagttgcatg ataaagaaga tagtcataag tgcggcgacg
     3241 atagtcatgc cccgcgccca ccggaaggag ctgactgggt tgaaggctct caagggcatc
     3301 ggtcgagatc ccggtgccta atgagtgagc taacttacat taattgcgtt gcgctcactg
     3361 cccgctttcc agtcgggaaa cctgtcgtgc cagctgcatt aatgaatcgg ccaacgcgcg
     3421 gggagaggcg gtttgcgtat tgggcgccag ggtggttttt cttttcacca gtgagacggg
     3481 caacagctga ttgcccttca ccgcctggcc ctgagagagt tgcagcaagc ggtccacgct
     3541 ggtttgcccc agcaggcgaa aatcctgttt gatggtggtt aacggcggga tataacatga
     3601 gctatcttcg gtatcgtcgt atcccactac cgagatgtcc gcaccaacgc gcagcccgga
     3661 ctcggtaatg gcgcgcattg cgcccagcgc catctgatcg ttggcaacca gcatcgcagt
     3721 gggaacgatg ccctcattca gcatttgcat ggtttgttga aaaccggaca tggcactcca
     3781 gtcgccttcc cgttccgcta tcggctgaat ttgattgcga gtgagatatt tatgccagcc
     3841 agccagacgc agacgcgccg agacagaact taatgggccc gctaacagcg cgatttgctg
     3901 gtgacccaat gcgaccagat gctccacgcc cagtcgcgta ccatcttcat gggagaaaat
     3961 aatactgttg atgggtgtct ggtcagagac atcaagaaat aacgccggaa cattagtgca
     4021 ggcagcttcc acagcaatgg catcctggtc atccagcgga tagttaatga tcagcccact
     4081 gacgcgttgc gcgagaagat tgtgcaccgc cgctttacag gcttcgacgc cgcttcgttc
     4141 taccatcgac accaccacgc tggcacccag ttgatcggcg cgagatttaa tcgccgcgac
     4201 aatttgcgac ggcgcgtgca gggccagact ggaggtggca acgccaatca gcaacgactg
     4261 tttgcccgcc agttgttgtg ccacgcggtt gggaatgtaa ttcagctccg ccatcgccgc
     4321 ttccactttt tcccgcgttt tcgcagaaac gtggctggcc tggttcacca cgcgggaaac
     4381 ggtctgataa gagacaccgg catactctgc gacatcgtat aacgttactg gtttcacatt
     4441 caccaccctg aattgactct cttccgggcg ctatcatgcc ataccgcgaa aggttttgcg
     4501 ccattcgatg gtgtccggga tctcgacgct ctcccttatg cgactcctgc attaggaagc
     4561 agcccagtag taggttgagg ccgttgagca ccgccgccgc aaggaatggt gcatgcaagg
     4621 agatggcgcc caacagtccc ccggccacgg ggcctgccac catacccacg ccgaaacaag
     4681 cgctcatgag cccgaagtgg cgagcccgat cttccccatc ggtgatgtcg gcgatatagg
     4741 cgccagcaac cgcacctgtg gcgccggtga tgccggccac gatgcgtccg gcgtagagga
     4801 tcgagatcga tctcgatccc gcgaaattaa tacgactcac tataggggaa ttgtgagcgg
     4861 ataacaattc ccctctagaa ataattttgt ttaactttaa gaaggagata tacatatgtt
     4921 cactatagac gagatgctat caaagaacaa ccaaagattg gctttcgagc acttcgctac
     4981 taagaacgac ggatgtggcc ctgatggtat gcacgtatct gagctggaga aatattggcg
     5041 tatgaatcac gatcagatca tctccgactt gaagaaccaa gagtaccaac ccggcattat
     5101 tttgattaga gaacacatga acaaaacagg taagagaagg aacattgcat ctctcaacgt
     5161 gatcgacagg ttcataactc gactgctgtc gcaaaagttg aatagatact tagcccccat
     5221 tttctgcgag aactcatacg cttaccaaga ctcaaaaggg gtgatgcccg ccgtactaaa
     5281 agccaaggag tacgtcgagt tgggtatgag acacgttatc gagatagacc tcaagaatta
     5341 cttcgacacg atcccgttag agaacttaat cccagagatc gagcgttaca taacagatga
     5401 ggcagtgctg cacttgatta aacagtactt attctgtgac atctcgttcg agggtaagat
     5461 atcacgtaaa actcaaggga tcgttcaggg taacgcaatt tccccaatac tatccaacct
     5521 ctacctgaac gacttcgaca aggagctgga cgagtcgaag ctgtgctgga tcagatacgc
     5581 cgacaacata tacatctata tggacagcta cgagaaggcc ctactggttt attccgagct
     5641 taccgagaga cttgagagaa ggaaattaac cgttaacaag gagaaatcag gggtgtttga
     5701 tgttagtacc cgttctatac taggttacga catcctgatc agaaacaaga aggttgacgt
     5761 gcgtaaacac atctacaagt ctgttaacca gtactccaac tggcacgact ctcgattgga
     5821 gttcatcaac ggtcgttacc acatcacttc cgacggtatc ctcaatagac aagacttcgg
     5881 tctactgttc gagaatgagc agaagaagca ctacatacct gttgaggttt ctgaccagtt
     5941 gaatatttac ggaaacgtta cgctcgccag caacgttctt caatcattct ccaaccgaga
     6001 gatcaaagtg tccttcttcg acaagtacgg gagacttatt ggatctttcc tgcccgagaa
     6061 aactaagaaa agcgccgaga taatactggt tcagtctaag aactacctta acgaggacgt
     6121 taggatggac acggcccgac gtatggagat cgccggtttg cataacatca gagccaacct
     6181 cagatactac gacaagaagc acaaaggtga ctttaaggaa aaggtagacg ccatttctgg
     6241 ctatattgac gccctcaata gagcaccatc cgtgaacgac atgatgctct tagaggctaa
     6301 agctcgtcag ctgtactaca catgcttcaa ccagatctta gagacatccg actttcagtt
     6361 cgagaagcgt accaagcgac caccaaaaga cgccatcaac gcctgtattt cattcggcaa
     6421 cacattactg tacaacttgt ttgtgaatat catttggaag aagggacttg atccaaggtt
     6481 cggggttgtt cacgccagca acaagcgtaa tcagtccctt aacctggatt ttgctgacat
     6541 cttcaagcct atagtcatag ataggatcat cttcacaatg atcaacaaga agatgctgac
     6601 cttgttgacg gacttcgaga cttctaacca gggagtttac ctttcccgag aggggaagaa
     6661 catcttcttg cagatgtacg aagaaaagct caaatccaga atcactatca agggaaagga
     6721 gatgagttac tatcagttgc tagaatcaga ggtgcagaac tacaagaact tcatcctgac
     6781 cggcgagaca tacaagccgt acaagtacta ctagcgtacg ttaactttaa gaaggagaac
     6841 ttaagatgta tgtcattcta gtttacgaca ttcaccagaa gcgagtaggt aaggctctaa
     6901 agatttgtag gaagtacctg atccacatcc agaagtcagt tttcgaaggc aacataacgg
     6961 agagtaagct aaaggcctta aaagaggagc tggggcacct gatcgacact cagatggact
     7021 cggtaattat ctaccacctg gactccgtga agtacactaa gaaggagcag atcggcatcg
     7081 tccaatcgac atccaatgtc atttgactcg agatccggct gctaacaaag cccgaaagga
     7141 agctgagttg gctgctgcca ccgctgagca ataactagca taaccccttg gggcctctaa
     7201 acgggtcttg aggggttttt tgctgaaagg aggaactata tccggata
//
