LOCUS       pFS_0355_pET30-R        7363 bp    DNA     circular UNA 25-AUG-2018
DEFINITION  Concatenation of 2 sequences.
ACCESSION   urn.local...2c-9fa11gd
VERSION     urn.local...2c-9fa11gd
KEYWORDS    .
SOURCE      
  ORGANISM  
            .
FEATURES             Location/Qualifiers
     promoter        7..156
                     /created_by="User"
                     /label="Leader"
     repeat_region   157..192
                     /created_by="User"
                     /label="DR"
     misc_feature    193..197
                     /created_by="User"
                     /label="FaqI"
     promoter        379..407
                     /Imported_from="<a
                     href=""http://wishart.biology.ualberta.ca/PlasMapper"">Pla
                     sMapper</a>"
                     /Transferred_From="amp prom"
                     /Transferred_Similarity="89.66%"
                     /label="amp prom"
     marker          complement(410..1225)
                     /Imported_from="<a
                     href=""http://wishart.biology.ualberta.ca/PlasMapper"">Pla
                     sMapper</a>"
                     /Transferred_From="kan2 marker"
                     /Transferred_Similarity="99.75%"
                     /label="kan2 marker"
     misc_feature    complement(411..1225)
                     /note="KanR"
                     /label="KanR"
     misc_feature    1331..1950
                     /Imported_from="<a
                     href=""http://wishart.biology.ualberta.ca/PlasMapper"">Pla
                     sMapper</a>"
                     /Transferred_From="pBR322 origin"
                     /Transferred_Similarity="99.68%"
                     /label="pBR322 origin"
                     /note="Geneious type: origin of replication"
     gene            complement(2362..2553)
                     /Imported_from="<a
                     href=""http://wishart.biology.ualberta.ca/PlasMapper"">Pla
                     sMapper</a>"
                     /Transferred_From="ROP"
                     /Transferred_Similarity="100.00%"
                     /label="ROP"
     misc_feature    complement(3362..4453)
                     /Imported_from="<a
                     href=""http://wishart.biology.ualberta.ca/PlasMapper"">Pla
                     sMapper</a>"
                     /Transferred_From="lacI reg"
                     /Transferred_Similarity="99.91%"
                     /label="lacI reg"
                     /note="Geneious type: regulatory sequence"
     promoter        4835..4853
                     /Imported_from="<a
                     href=""http://wishart.biology.ualberta.ca/PlasMapper"">Pla
                     sMapper</a>"
                     /Transferred_From="T7 prom"
                     /Transferred_Similarity="100.00%"
                     /label="T7 prom"
     misc_feature    4853..4880
                     /Imported_from="<a
                     href=""http://wishart.biology.ualberta.ca/PlasMapper"">Pla
                     sMapper</a>"
                     /Transferred_From="lacO reg"
                     /Transferred_Similarity="100.00%"
                     /label="lacO reg"
                     /note="Geneious type: regulatory sequence"
     gene            4898..4914
                     /Imported_from="<a
                     href=""http://wishart.biology.ualberta.ca/PlasMapper"">Pla
                     sMapper</a>"
                     /Transferred_From="T7_transl_en_RBS"
                     /Transferred_Similarity="100.00%"
                     /label="T7_transl_en_RBS"
     CDS             4923..6905
                     /created_by="User"
                     /label="Cas1"
     gene            6912..6928
                     /Imported_from="<a
                     href=""http://wishart.biology.ualberta.ca/PlasMapper"">Pla
                     sMapper</a>"
                     /X-Transferred_From="T7_transl_en_RBS"
                     /X-Transferred_Similarity="100.00%"
                     /Transferred_From="Digestion of
                     pFSNW_013_pET30bvectorNovagen_Golden Gate_T7_Terminator -
                     Fragment 2, Ligated sequences"
                     /Transferred_Similarity="100.00%"
                     /label="T7_transl_en_RBS"
     CDS             6937..7221
                     /created_by="User"
                     /label="Cas2"
     misc_feature    <7223..>7226
                     /note="XhoI"
                     /label="XhoI"
     terminator      7234..7362
                     /created_by="User"
                     /label="Terminator extended"
     terminator      7295..7338
                     /Imported_from="<a
                     href=""http://wishart.biology.ualberta.ca/PlasMapper"">Pla
                     sMapper</a>"
                     /Transferred_From="T7 term"
                     /Transferred_Similarity="97.73%"
                     /label="T7 term"
     misc_feature    complement(7363..>5)
                     /label="CsiI"
ORIGIN      
        1 ccaggtaaaa tacttcggca tacgggcgcc gcgtgttgat tttcaaggga aaagaggatt
       61 ttcgtgcgcc tggacgggag cgcgggagcg ttgtttcgcc cgcgccccga aaagagccgt
      121 taaattcctt gacgatcatc gggttgtgga agtagagtga aaactaagcc ctgccgataa
      181 agggattgag acgtcccgcg gccgctattc ttttgattta taagggattt tgccgatttc
      241 ggcctattgg ttaaaaaatg agctgattta acaaaaattt aacgcgaatt ttaacaaaat
      301 attaacgctt acaatttcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg
      361 tttatttttc taaatacatt caaatatgta tccgctcatg aattaattct tagaaaaact
      421 catcgagcat caaatgaaac tgcaatttat tcatatcagg attatcaata ccatattttt
      481 gaaaaagccg tttctgtaat gaaggagaaa actcaccgag gcagttccat aggatggcaa
      541 gatcctggta tcggtctgcg attccgactc gtccaacatc aatacaacct attaatttcc
      601 cctcgtcaaa aataaggtta tcaagtgaga aatcaccatg agtgacgact gaatccggtg
      661 agaatggcaa aagtttatgc atttctttcc agacttgttc aacaggccag ccattacgct
      721 cgtcatcaaa atcactcgca tcaaccaaac cgttattcat tcgtgattgc gcctgagcga
      781 gacgaaatac gcgatcgctg ttaaaaggac aattacaaac aggaatcgaa tgcaaccggc
      841 gcaggaacac tgccagcgca tcaacaatat tttcacctga atcaggatat tcttctaata
      901 cctggaatgc tgttttcccg gggatcgcag tggtgagtaa ccatgcatca tcaggagtac
      961 ggataaaatg cttgatggtc ggaagaggca taaattccgt cagccagttt agtctgacca
     1021 tctcatctgt aacatcattg gcaacgctac ctttgccatg tttcagaaac aactctggcg
     1081 catcgggctt cccatacaat cgatagattg tcgcacctga ttgcccgaca ttatcgcgag
     1141 cccatttata cccatataaa tcagcatcca tgttggaatt taatcgcggc ctagagcaag
     1201 acgtttcccg ttgaatatgg ctcataacac cccttgtatt actgtttatg taagcagaca
     1261 gttttattgt tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac
     1321 cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc
     1381 ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca
     1441 actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgtccttcta
     1501 gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct
     1561 ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg
     1621 gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc
     1681 acacagccca gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta
     1741 tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg
     1801 gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt
     1861 cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg
     1921 cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggt cttttgctgg
     1981 ccttttgctc acatgttctt tcctgcgtta tcccctgatt ctgtggataa ccgtattacc
     2041 gcctttgagt gagctgatac cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg
     2101 agcgaggaag cggaagagcg cctgatgcgg tattttctcc ttacgcatct gtgcggtatt
     2161 tcacaccgca atggtgcact ctcagtacaa tctgctctga tgccgcatag ttaagccagt
     2221 atacactccg ctatcgctac gtgactgggt catggctgcg ccccgacacc cgccaacacc
     2281 cgctgacgcg ccctgacggg cttgtctgct cccggcatcc gcttacagac aagctgtgac
     2341 cgtctccggg agctgcatgt gtcagaggtt ttcaccgtca tcaccgaaac gcgcgaggca
     2401 gctgcggtaa agctcatcag cgtggtcgtg aagcgattca cagatgtctg cctgttcatc
     2461 cgcgtccagc tcgttgagtt tctccagaag cgttaatgtc tggcttctga taaagcgggc
     2521 catgttaagg gcggtttttt cctgtttggt cactgatgcc tccgtgtaag ggggatttct
     2581 gttcatgggg gtaatgatac cgatgaaacg agagaggatg ctcacgatac gggttactga
     2641 tgatgaacat gcccggttac tggaacgttg tgagggtaaa caactggcgg tatggatgcg
     2701 gcgggaccag agaaaaatca ctcagggtca atgccagcgc ttcgttaata cagatgtagg
     2761 tgttccacag ggtagccagc agcatcctgc gatgcagatc cggaacataa tggtgcaggg
     2821 cgctgacttc cgcgtttcca gactttacga aacacggaaa ccgaagatca ttcatgttgt
     2881 tgctcaggtc gcagacgttt tgcagcagca gtcgcttcac gttcgctcgc gtatcggtga
     2941 ttcattctgc taaccagtaa ggcaaccccg ccagcctagc cgggtcctca acgacaggag
     3001 cacgatcatg cgcacccgtg gggccgccat gccggcgata atggcctgct tctcgccgaa
     3061 acgtttggtg gcgggaccag tgacgaaggc ttgagcgagg gcgtgcaaga ttccgaatac
     3121 cgcaagcgac aggccgatca tcgtcgcgct ccagcgaaag cggtcctcgc cgaaaatgac
     3181 ccagagcgct gccggcacct gtcctacgag ttgcatgata aagaagatag tcataagtgc
     3241 ggcgacgata gtcatgcccc gcgcccaccg gaaggagctg actgggttga aggctctcaa
     3301 gggcatcggt cgagatcccg gtgcctaatg agtgagctaa cttacattaa ttgcgttgcg
     3361 ctcactgccc gctttccagt cgggaaacct gtcgtgccag ctgcattaat gaatcggcca
     3421 acgcgcgggg agaggcggtt tgcgtattgg gcgccagggt ggtttttctt ttcaccagtg
     3481 agacgggcaa cagctgattg cccttcaccg cctggccctg agagagttgc agcaagcggt
     3541 ccacgctggt ttgccccagc aggcgaaaat cctgtttgat ggtggttaac ggcgggatat
     3601 aacatgagct atcttcggta tcgtcgtatc ccactaccga gatgtccgca ccaacgcgca
     3661 gcccggactc ggtaatggcg cgcattgcgc ccagcgccat ctgatcgttg gcaaccagca
     3721 tcgcagtggg aacgatgccc tcattcagca tttgcatggt ttgttgaaaa ccggacatgg
     3781 cactccagtc gccttcccgt tccgctatcg gctgaatttg attgcgagtg agatatttat
     3841 gccagccagc cagacgcaga cgcgccgaga cagaacttaa tgggcccgct aacagcgcga
     3901 tttgctggtg acccaatgcg accagatgct ccacgcccag tcgcgtacca tcttcatggg
     3961 agaaaataat actgttgatg ggtgtctggt cagagacatc aagaaataac gccggaacat
     4021 tagtgcaggc agcttccaca gcaatggcat cctggtcatc cagcggatag ttaatgatca
     4081 gcccactgac gcgttgcgcg agaagattgt gcaccgccgc tttacaggct tcgacgccgc
     4141 ttcgttctac catcgacacc accacgctgg cacccagttg atcggcgcga gatttaatcg
     4201 ccgcgacaat ttgcgacggc gcgtgcaggg ccagactgga ggtggcaacg ccaatcagca
     4261 acgactgttt gcccgccagt tgttgtgcca cgcggttggg aatgtaattc agctccgcca
     4321 tcgccgcttc cactttttcc cgcgttttcg cagaaacgtg gctggcctgg ttcaccacgc
     4381 gggaaacggt ctgataagag acaccggcat actctgcgac atcgtataac gttactggtt
     4441 tcacattcac caccctgaat tgactctctt ccgggcgcta tcatgccata ccgcgaaagg
     4501 ttttgcgcca ttcgatggtg tccgggatct cgacgctctc ccttatgcga ctcctgcatt
     4561 aggaagcagc ccagtagtag gttgaggccg ttgagcaccg ccgccgcaag gaatggtgca
     4621 tgcaaggaga tggcgcccaa cagtcccccg gccacggggc ctgccaccat acccacgccg
     4681 aaacaagcgc tcatgagccc gaagtggcga gcccgatctt ccccatcggt gatgtcggcg
     4741 atataggcgc cagcaaccgc acctgtggcg ccggtgatgc cggccacgat gcgtccggcg
     4801 tagaggatcg agatcgatct cgatcccgcg aaattaatac gactcactat aggggaattg
     4861 tgagcggata acaattcccc tctagaaata attttgttta actttaagaa ggagatatac
     4921 atatgtctac tttacccacc ccttccagca ctgaccaaga ctcaccacca cctttctgga
     4981 cattagctag gttggcagaa gctcttgagc acgtgtcagc tcgacaaggc ggagctggag
     5041 ctgatgagca aactttggct gagttcgcag cagacgcaga ggcccagtta gggcttttgg
     5101 ccttgcagct aacacaagga agttacagac cagcccctgc tagacttata ccagtcgcta
     5161 aaccaggagg aggtgttcgt gagcttttac tgccagcagt ccgagataga attgtccaaa
     5221 gtgctttggc tcgttacctt gctgaccttt tggaaccaga ttttggtgag gctagtcacg
     5281 cttacagacc gggacactcc gtagctactg ctttacatag gcttcaagcc ttacgagatg
     5341 gcggattggt tttcgtggca gtctgtgata tacatcattt ctttgattcg gtagatcaca
     5401 gaaggctctt ttctctttta gatgaccttc cattggaaag acgtttgcga gagcaaatga
     5461 aaacttgtgt tcgtatagag gtagctgatg tacaaggcca gggagcttgg tccttagcta
     5521 gaggattggc ccaaggttct cctctgtctc cagtcttagc taacttgttc ttgatggctt
     5581 ttgatgctgc atgtgctcgt gctgggttgg ctcttgtcag atatgctgat gactgtgtcc
     5641 ttgcttgcgc ttccgagact gaagcccagt ccgccctagc atttgctgct gatgctcttg
     5701 agaatattgg gttggccctt aataccagaa agtcgcgtct tgcctctttc gcagaagggt
     5761 ttgagtttct aggtgcattt tgtggagctg agggcatgtt gggtggccga ccaggagaag
     5821 cagcttgttt accaccaact accggaccag tacatgaggc tgctgctgct gacgatgaga
     5881 ggccaccttc acatggtcat cgtccaaggt tacgtacatt gtacttgttg gagaatggcg
     5941 cagttctcaa taaagaaggg gagagattta tagtagctcg acatggtgag gtattacttc
     6001 aggttcctat gatgcgtatt gatcaaatta tggtctttgg taatgtccaa attactactc
     6061 cagctttgca tgagtgttta gaaagaggaa tccctgttat gcttctaagt ggtcgaggac
     6121 gtttctttgg tgttattgat ccccttgatg caagatccgt ccctttacaa agggctcaat
     6181 tcgcactaga atctgatgaa ccagctagac tagccctcgc taggccttta attgctggaa
     6241 agattttgaa ttgtagaacc ttcttgggaa gattggctag agccagacaa acaaatatgg
     6301 acgctccatt agctgctctc aagagcgcag cccaagcagc cggacaagct gccgatttgg
     6361 aaattttgag agggattgag ggagcagcag ctagaacata ctttgcagct tggcagactg
     6421 tcttaccagc aaaatggcag tttactggta gaaataggca accgccaact gatcctgtaa
     6481 atgccctgct gagttacggt tatacaatcg tcttttataa cgtgctggca ttggttagag
     6541 caagaggact aaatactcat gttggggtat tgcatgacgt tagacctgga caccccgcct
     6601 tagcttcgga cttaatggaa gaatttcgtg caccagttgt tgacgcagtt gttatgcact
     6661 tggttttcga tggaaaattg cagccaggtg atttcagctg gccagaaacc cccggacaac
     6721 cctgtctcat ggcagacggc tcacgaaaac actttattca cttattagaa cagaaattga
     6781 atacaacagt ttctcatgct ggtcagcgac ttgactatag gagatggatg gatatgcaag
     6841 ttcttcaata tgcagcagct cttagaactc cagggttgcc gtatgttccc tttgctatta
     6901 gataacgtac gttaacttta agaaggagaa cttaagatgg agcaaacgtg gctggttacg
     6961 tatgatgtaa gtgatgatgg ttgtagaagg agagtagaaa ggatattgtt gggtcatgga
     7021 gagcgtgagc aatatagcgt gtttaggtgt agactatctt ctagggaagt tagggattta
     7081 cgagctagat tggcaggaca cttgaagggt tctgatagta ttcgatacta ccccctttgt
     7141 gctgcatgtt taccaaggca accccaacgt accctagtcg attcagctga ggctggtatc
     7201 gcttggtatt tcgcagttta actcgagatc cggctgctaa caaagcccga aaggaagctg
     7261 agttggctgc tgccaccgct gagcaataac tagcataacc ccttggggcc tctaaacggg
     7321 tcttgagggg ttttttgctg aaaggaggaa ctatatccgg ata
//
