LOCUS       pFS_0358_pET30-R        7061 bp    DNA     circular UNA 25-AUG-2018
DEFINITION  Concatenation of 2 sequences.
ACCESSION   urn.local...2f-9fa11ge
VERSION     urn.local...2f-9fa11ge
KEYWORDS    .
SOURCE      
  ORGANISM  
            .
FEATURES             Location/Qualifiers
     promoter        7..156
                     /created_by="User"
                     /label="Leader"
     repeat_region   157..193
                     /created_by="User"
                     /label="DR"
     misc_feature    194..198
                     /created_by="User"
                     /label="FaqI"
     promoter        380..408
                     /Imported_from="<a
                     href=""http://wishart.biology.ualberta.ca/PlasMapper"">Pla
                     sMapper</a>"
                     /Transferred_From="amp prom"
                     /Transferred_Similarity="89.66%"
                     /label="amp prom"
     marker          complement(411..1226)
                     /Imported_from="<a
                     href=""http://wishart.biology.ualberta.ca/PlasMapper"">Pla
                     sMapper</a>"
                     /Transferred_From="kan2 marker"
                     /Transferred_Similarity="99.75%"
                     /label="kan2 marker"
     misc_feature    complement(412..1226)
                     /note="KanR"
                     /label="KanR"
     misc_feature    1332..1951
                     /Imported_from="<a
                     href=""http://wishart.biology.ualberta.ca/PlasMapper"">Pla
                     sMapper</a>"
                     /Transferred_From="pBR322 origin"
                     /Transferred_Similarity="99.68%"
                     /label="pBR322 origin"
                     /note="Geneious type: origin of replication"
     gene            complement(2363..2554)
                     /Imported_from="<a
                     href=""http://wishart.biology.ualberta.ca/PlasMapper"">Pla
                     sMapper</a>"
                     /Transferred_From="ROP"
                     /Transferred_Similarity="100.00%"
                     /label="ROP"
     misc_feature    complement(3363..4454)
                     /Imported_from="<a
                     href=""http://wishart.biology.ualberta.ca/PlasMapper"">Pla
                     sMapper</a>"
                     /Transferred_From="lacI reg"
                     /Transferred_Similarity="99.91%"
                     /label="lacI reg"
                     /note="Geneious type: regulatory sequence"
     promoter        4836..4854
                     /Imported_from="<a
                     href=""http://wishart.biology.ualberta.ca/PlasMapper"">Pla
                     sMapper</a>"
                     /Transferred_From="T7 prom"
                     /Transferred_Similarity="100.00%"
                     /label="T7 prom"
     misc_feature    4854..4881
                     /Imported_from="<a
                     href=""http://wishart.biology.ualberta.ca/PlasMapper"">Pla
                     sMapper</a>"
                     /Transferred_From="lacO reg"
                     /Transferred_Similarity="100.00%"
                     /label="lacO reg"
                     /note="Geneious type: regulatory sequence"
     gene            4899..4915
                     /Imported_from="<a
                     href=""http://wishart.biology.ualberta.ca/PlasMapper"">Pla
                     sMapper</a>"
                     /Transferred_From="T7_transl_en_RBS"
                     /Transferred_Similarity="100.00%"
                     /label="T7_transl_en_RBS"
     CDS             4924..6600
                     /created_by="User"
                     /label="Cas1"
     gene            6607..6623
                     /Imported_from="<a
                     href=""http://wishart.biology.ualberta.ca/PlasMapper"">Pla
                     sMapper</a>"
                     /X-Transferred_From="T7_transl_en_RBS"
                     /X-Transferred_Similarity="100.00%"
                     /Transferred_From="Digestion of
                     pFSNW_013_pET30bvectorNovagen_Golden Gate_T7_Terminator -
                     Fragment 2, Ligated sequences, Ligated sequences 2"
                     /Transferred_Similarity="100.00%"
                     /label="T7_transl_en_RBS"
     CDS             6632..6919
                     /created_by="User"
                     /label="Cas2"
     misc_feature    <6921..>6924
                     /note="XhoI"
                     /label="XhoI"
     terminator      6932..7060
                     /created_by="User"
                     /label="Terminator extended"
     terminator      6993..7036
                     /Imported_from="<a
                     href=""http://wishart.biology.ualberta.ca/PlasMapper"">Pla
                     sMapper</a>"
                     /Transferred_From="T7 term"
                     /Transferred_Similarity="97.73%"
                     /label="T7 term"
     misc_feature    complement(7061..>5)
                     /label="CsiI"
ORIGIN      
        1 ccaggtcaga atttgcgatc cctccagggt tgtacctctt tctggacctg gagccggagg
       61 atcgttgcga tcctttcagg ggtgatgagc gacaagcagg ttgggcggtg ggtcgcgatg
      121 ttatcgaggg cgaactccct cccacgctta gcaacagttt ctatgcccgc tgggttgggc
      181 tgtggatggc gatgtcccgc ggccgctatt cttttgattt ataagggatt ttgccgattt
      241 cggcctattg gttaaaaaat gagctgattt aacaaaaatt taacgcgaat tttaacaaaa
      301 tattaacgct tacaatttca ggtggcactt ttcggggaaa tgtgcgcgga acccctattt
      361 gtttattttt ctaaatacat tcaaatatgt atccgctcat gaattaattc ttagaaaaac
      421 tcatcgagca tcaaatgaaa ctgcaattta ttcatatcag gattatcaat accatatttt
      481 tgaaaaagcc gtttctgtaa tgaaggagaa aactcaccga ggcagttcca taggatggca
      541 agatcctggt atcggtctgc gattccgact cgtccaacat caatacaacc tattaatttc
      601 ccctcgtcaa aaataaggtt atcaagtgag aaatcaccat gagtgacgac tgaatccggt
      661 gagaatggca aaagtttatg catttctttc cagacttgtt caacaggcca gccattacgc
      721 tcgtcatcaa aatcactcgc atcaaccaaa ccgttattca ttcgtgattg cgcctgagcg
      781 agacgaaata cgcgatcgct gttaaaagga caattacaaa caggaatcga atgcaaccgg
      841 cgcaggaaca ctgccagcgc atcaacaata ttttcacctg aatcaggata ttcttctaat
      901 acctggaatg ctgttttccc ggggatcgca gtggtgagta accatgcatc atcaggagta
      961 cggataaaat gcttgatggt cggaagaggc ataaattccg tcagccagtt tagtctgacc
     1021 atctcatctg taacatcatt ggcaacgcta cctttgccat gtttcagaaa caactctggc
     1081 gcatcgggct tcccatacaa tcgatagatt gtcgcacctg attgcccgac attatcgcga
     1141 gcccatttat acccatataa atcagcatcc atgttggaat ttaatcgcgg cctagagcaa
     1201 gacgtttccc gttgaatatg gctcataaca ccccttgtat tactgtttat gtaagcagac
     1261 agttttattg ttcatgacca aaatccctta acgtgagttt tcgttccact gagcgtcaga
     1321 ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg
     1381 cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc
     1441 aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgtccttct
     1501 agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc
     1561 tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt
     1621 ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg
     1681 cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagct
     1741 atgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg taagcggcag
     1801 ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag
     1861 tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg
     1921 gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg tcttttgctg
     1981 gccttttgct cacatgttct ttcctgcgtt atcccctgat tctgtggata accgtattac
     2041 cgcctttgag tgagctgata ccgctcgccg cagccgaacg accgagcgca gcgagtcagt
     2101 gagcgaggaa gcggaagagc gcctgatgcg gtattttctc cttacgcatc tgtgcggtat
     2161 ttcacaccgc aatggtgcac tctcagtaca atctgctctg atgccgcata gttaagccag
     2221 tatacactcc gctatcgcta cgtgactggg tcatggctgc gccccgacac ccgccaacac
     2281 ccgctgacgc gccctgacgg gcttgtctgc tcccggcatc cgcttacaga caagctgtga
     2341 ccgtctccgg gagctgcatg tgtcagaggt tttcaccgtc atcaccgaaa cgcgcgaggc
     2401 agctgcggta aagctcatca gcgtggtcgt gaagcgattc acagatgtct gcctgttcat
     2461 ccgcgtccag ctcgttgagt ttctccagaa gcgttaatgt ctggcttctg ataaagcggg
     2521 ccatgttaag ggcggttttt tcctgtttgg tcactgatgc ctccgtgtaa gggggatttc
     2581 tgttcatggg ggtaatgata ccgatgaaac gagagaggat gctcacgata cgggttactg
     2641 atgatgaaca tgcccggtta ctggaacgtt gtgagggtaa acaactggcg gtatggatgc
     2701 ggcgggacca gagaaaaatc actcagggtc aatgccagcg cttcgttaat acagatgtag
     2761 gtgttccaca gggtagccag cagcatcctg cgatgcagat ccggaacata atggtgcagg
     2821 gcgctgactt ccgcgtttcc agactttacg aaacacggaa accgaagatc attcatgttg
     2881 ttgctcaggt cgcagacgtt ttgcagcagc agtcgcttca cgttcgctcg cgtatcggtg
     2941 attcattctg ctaaccagta aggcaacccc gccagcctag ccgggtcctc aacgacagga
     3001 gcacgatcat gcgcacccgt ggggccgcca tgccggcgat aatggcctgc ttctcgccga
     3061 aacgtttggt ggcgggacca gtgacgaagg cttgagcgag ggcgtgcaag attccgaata
     3121 ccgcaagcga caggccgatc atcgtcgcgc tccagcgaaa gcggtcctcg ccgaaaatga
     3181 cccagagcgc tgccggcacc tgtcctacga gttgcatgat aaagaagata gtcataagtg
     3241 cggcgacgat agtcatgccc cgcgcccacc ggaaggagct gactgggttg aaggctctca
     3301 agggcatcgg tcgagatccc ggtgcctaat gagtgagcta acttacatta attgcgttgc
     3361 gctcactgcc cgctttccag tcgggaaacc tgtcgtgcca gctgcattaa tgaatcggcc
     3421 aacgcgcggg gagaggcggt ttgcgtattg ggcgccaggg tggtttttct tttcaccagt
     3481 gagacgggca acagctgatt gcccttcacc gcctggccct gagagagttg cagcaagcgg
     3541 tccacgctgg tttgccccag caggcgaaaa tcctgtttga tggtggttaa cggcgggata
     3601 taacatgagc tatcttcggt atcgtcgtat cccactaccg agatgtccgc accaacgcgc
     3661 agcccggact cggtaatggc gcgcattgcg cccagcgcca tctgatcgtt ggcaaccagc
     3721 atcgcagtgg gaacgatgcc ctcattcagc atttgcatgg tttgttgaaa accggacatg
     3781 gcactccagt cgccttcccg ttccgctatc ggctgaattt gattgcgagt gagatattta
     3841 tgccagccag ccagacgcag acgcgccgag acagaactta atgggcccgc taacagcgcg
     3901 atttgctggt gacccaatgc gaccagatgc tccacgccca gtcgcgtacc atcttcatgg
     3961 gagaaaataa tactgttgat gggtgtctgg tcagagacat caagaaataa cgccggaaca
     4021 ttagtgcagg cagcttccac agcaatggca tcctggtcat ccagcggata gttaatgatc
     4081 agcccactga cgcgttgcgc gagaagattg tgcaccgccg ctttacaggc ttcgacgccg
     4141 cttcgttcta ccatcgacac caccacgctg gcacccagtt gatcggcgcg agatttaatc
     4201 gccgcgacaa tttgcgacgg cgcgtgcagg gccagactgg aggtggcaac gccaatcagc
     4261 aacgactgtt tgcccgccag ttgttgtgcc acgcggttgg gaatgtaatt cagctccgcc
     4321 atcgccgctt ccactttttc ccgcgttttc gcagaaacgt ggctggcctg gttcaccacg
     4381 cgggaaacgg tctgataaga gacaccggca tactctgcga catcgtataa cgttactggt
     4441 ttcacattca ccaccctgaa ttgactctct tccgggcgct atcatgccat accgcgaaag
     4501 gttttgcgcc attcgatggt gtccgggatc tcgacgctct cccttatgcg actcctgcat
     4561 taggaagcag cccagtagta ggttgaggcc gttgagcacc gccgccgcaa ggaatggtgc
     4621 atgcaaggag atggcgccca acagtccccc ggccacgggg cctgccacca tacccacgcc
     4681 gaaacaagcg ctcatgagcc cgaagtggcg agcccgatct tccccatcgg tgatgtcggc
     4741 gatataggcg ccagcaaccg cacctgtggc gccggtgatg ccggccacga tgcgtccggc
     4801 gtagaggatc gagatcgatc tcgatcccgc gaaattaata cgactcacta taggggaatt
     4861 gtgagcggat aacaattccc ctctagaaat aattttgttt aactttaaga aggagatata
     4921 catatgacca gggtgtctat ccctaaaccc gatggcggaa tacgtagctt ggctatagga
     4981 gctattgaag atagaattgt cgaaagagct gttctggatg ttcttgatcc agttgtggac
     5041 cccacacttt ccccgtggag ttttgcatac aggagaggct taggcgtgag ggatgcagtt
     5101 cgagctttgg cagaagctag ggaatctggt ctagcttttg ttgttcgttg tgatattgat
     5161 gattgttttg attcgatccc gagatggcct ctacttagga gactaagaga attggtttct
     5221 gacgctgaat tggttgcctt ggtagaaaga ttggttggca gaccagtcac tggagaaagg
     5281 gcttccggtg gtaggggatt gcatcagggc ggatcgctca gtcctttgtt ggccaaccta
     5341 tatttggata cctttgatcg agcattgatg cgtcatggac accgtgtagt tagatatgga
     5401 gatgatatag ctatatctgt tcccgatagg ccaactggac ttagagtatt ggatttggct
     5461 gatgcagaag cagaagccct gtctttgaga ctaaatactg atgatagaca agtcatagct
     5521 tttgatgaag gagtcccttt ctgtggtcag gttgttactg catcaagcgg gccaacagct
     5581 gatttacaag ctaaacctct gcaaggtaca gtctttgtta caacccaagg agcattgcta
     5641 agagtaaaag gagaacgact tcgtgtcgaa gatggagaca ggctactggc taatgtaaat
     5701 ttgaagcgtg tgaggcaaat agtctgtttt ggtagagtag gagtgacttc taccttgtta
     5761 caaagaattg tagaacgagg aatagaactt gcttggttat atgaggatgg tagacatgca
     5821 gctagagtaa gtgggttgga tggtactgat ccagaggtcc gtttagccca atatagagct
     5881 gctgatgatg cacgtcaggc actgagaata gctaggcagt tagtcgctgg taaagtaaca
     5941 aatatgagag ttggtctttt gcgagctgct agggctcaac aagctccaga gcttgctgat
     6001 cgtcaggcac gattggccac agctcgacag tcagctttga ttgcagattc tactgctgaa
     6061 ttgatgggtt atgagggttc agctactaga gattattttg ctggtctttc acaaattttg
     6121 ggtcctgagt ggggctttac tactcgtcaa agaaggcctc ctcctgaccc cgtgaatgcc
     6181 atgcttagtt ttggatatac cttgctgact aatgaggcac tcacggcctg tcagctggct
     6241 ggacttgatc catatctcgg tatgttacat tctcctagaa gaaatagacc ttctttggca
     6301 ttggatttaa tcgaagaact ccgacccgtt gttgttgatg ctaccgttat taggctagta
     6361 cgaacaggac aagttactcc taagaacttt actctgacag atgatagagg atgtagattg
     6421 gatgatcatg gtagacgtgc ctttctggat gcttatgagc gtagaatgct aacacttgtc
     6481 catcatcctg tagaacaaag aagaataccg tggagacatg ttttgttagc acaagctaga
     6541 acacttgctg ctgtattgtc atcgcgtcgt cccgaatata gacccgtagt atggcgttaa
     6601 cgtacgttaa ctttaagaag gagaacttaa gatgtcccgt gtttgttatg tagttgccta
     6661 tgatatagcc gatgacgacc gaagggccga tctcgctatg tttctttcag ggtacggtcc
     6721 gcgtgttcag ttgtctgtgt ttgaagtcga attgccggat gctgatacag ctgttgcttt
     6781 tagggagcgt ttgcgtagtt taatagaccc taacgacgat caagttcgtc tgtatcgact
     6841 cactccacag gcattgggac agagaattat ttacggaaga aggaccattg aggagagagt
     6901 tgacttttgg atcgtgtaac tcgagatccg gctgctaaca aagcccgaaa ggaagctgag
     6961 ttggctgctg ccaccgctga gcaataacta gcataacccc ttggggcctc taaacgggtc
     7021 ttgaggggtt ttttgctgaa aggaggaact atatccggat a
//
