LOCUS       pFS_0362_pET30-R        8020 bp    DNA     circular UNA 25-AUG-2018
DEFINITION  Concatenation of 2 sequences.
ACCESSION   urn.local...2j-9fa11gf
VERSION     urn.local...2j-9fa11gf
KEYWORDS    .
SOURCE      
  ORGANISM  
            .
FEATURES             Location/Qualifiers
     promoter        7..156
                     /created_by="User"
                     /label="Leader"
     repeat_region   157..192
                     /created_by="User"
                     /label="DR"
     misc_feature    193..197
                     /created_by="User"
                     /label="FaqI"
     promoter        379..407
                     /Imported_from="<a
                     href=""http://wishart.biology.ualberta.ca/PlasMapper"">Pla
                     sMapper</a>"
                     /Transferred_From="amp prom"
                     /Transferred_Similarity="89.66%"
                     /label="amp prom"
     marker          complement(410..1225)
                     /Imported_from="<a
                     href=""http://wishart.biology.ualberta.ca/PlasMapper"">Pla
                     sMapper</a>"
                     /Transferred_From="kan2 marker"
                     /Transferred_Similarity="99.75%"
                     /label="kan2 marker"
     misc_feature    complement(411..1225)
                     /note="KanR"
                     /label="KanR"
     misc_feature    1331..1950
                     /Imported_from="<a
                     href=""http://wishart.biology.ualberta.ca/PlasMapper"">Pla
                     sMapper</a>"
                     /Transferred_From="pBR322 origin"
                     /Transferred_Similarity="99.68%"
                     /label="pBR322 origin"
                     /note="Geneious type: origin of replication"
     gene            complement(2362..2553)
                     /Imported_from="<a
                     href=""http://wishart.biology.ualberta.ca/PlasMapper"">Pla
                     sMapper</a>"
                     /Transferred_From="ROP"
                     /Transferred_Similarity="100.00%"
                     /label="ROP"
     misc_feature    complement(3362..4453)
                     /Imported_from="<a
                     href=""http://wishart.biology.ualberta.ca/PlasMapper"">Pla
                     sMapper</a>"
                     /Transferred_From="lacI reg"
                     /Transferred_Similarity="99.91%"
                     /label="lacI reg"
                     /note="Geneious type: regulatory sequence"
     promoter        4835..4853
                     /Imported_from="<a
                     href=""http://wishart.biology.ualberta.ca/PlasMapper"">Pla
                     sMapper</a>"
                     /Transferred_From="T7 prom"
                     /Transferred_Similarity="100.00%"
                     /label="T7 prom"
     misc_feature    4853..4880
                     /Imported_from="<a
                     href=""http://wishart.biology.ualberta.ca/PlasMapper"">Pla
                     sMapper</a>"
                     /Transferred_From="lacO reg"
                     /Transferred_Similarity="100.00%"
                     /label="lacO reg"
                     /note="Geneious type: regulatory sequence"
     gene            4898..4914
                     /Imported_from="<a
                     href=""http://wishart.biology.ualberta.ca/PlasMapper"">Pla
                     sMapper</a>"
                     /Transferred_From="T7_transl_en_RBS"
                     /Transferred_Similarity="100.00%"
                     /label="T7_transl_en_RBS"
     misc_feature    4920..4925
                     /Recognition_pattern="CA^TATG"
                     /annotation_group="CATATG"
                     /label="NdeI"
                     /note="Geneious type: restriction site"
     CDS             4923..7565
                     /created_by="User"
                     /label="Cas1"
     gene            7572..7588
                     /Imported_from="<a
                     href=""http://wishart.biology.ualberta.ca/PlasMapper"">Pla
                     sMapper</a>"
                     /X-Transferred_From="T7_transl_en_RBS"
                     /X-Transferred_Similarity="100.00%"
                     /Transferred_From="Digestion of
                     pFSNW_013_pET30bvectorNovagen_Golden Gate_T7_Terminator -
                     Fragment 2, Ligated sequences"
                     /Transferred_Similarity="100.00%"
                     /label="T7_transl_en_RBS"
     misc_feature    7591..7596
                     /Recognition_pattern="C^TTAAG"
                     /annotation_group="CTTAAG"
                     /label="AflII"
                     /note="Geneious type: restriction site"
     CDS             7597..7878
                     /created_by="User"
                     /label="Cas2"
     misc_feature    <7880..>7883
                     /note="XhoI"
                     /label="XhoI"
     terminator      7891..8019
                     /created_by="User"
                     /label="Terminator extended"
     terminator      7952..7995
                     /Imported_from="<a
                     href=""http://wishart.biology.ualberta.ca/PlasMapper"">Pla
                     sMapper</a>"
                     /Transferred_From="T7 term"
                     /Transferred_Similarity="97.73%"
                     /label="T7 term"
     misc_feature    complement(8020..>5)
                     /label="CsiI"
ORIGIN      
        1 ccaggtggta ccccattttc ctgatgacga ttgggcatgg gtgacctgac tcttgccaaa
       61 ccctgtggat aagctgctag acttgccgga gtcatcgagg ttgggcaagc gacggtacgc
      121 aagtctccgg caagtcagcc ttttttgaca cccagtgctg aatagggaat cctgttcgaa
      181 caggcttgaa acgtcccgcg gccgctattc ttttgattta taagggattt tgccgatttc
      241 ggcctattgg ttaaaaaatg agctgattta acaaaaattt aacgcgaatt ttaacaaaat
      301 attaacgctt acaatttcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg
      361 tttatttttc taaatacatt caaatatgta tccgctcatg aattaattct tagaaaaact
      421 catcgagcat caaatgaaac tgcaatttat tcatatcagg attatcaata ccatattttt
      481 gaaaaagccg tttctgtaat gaaggagaaa actcaccgag gcagttccat aggatggcaa
      541 gatcctggta tcggtctgcg attccgactc gtccaacatc aatacaacct attaatttcc
      601 cctcgtcaaa aataaggtta tcaagtgaga aatcaccatg agtgacgact gaatccggtg
      661 agaatggcaa aagtttatgc atttctttcc agacttgttc aacaggccag ccattacgct
      721 cgtcatcaaa atcactcgca tcaaccaaac cgttattcat tcgtgattgc gcctgagcga
      781 gacgaaatac gcgatcgctg ttaaaaggac aattacaaac aggaatcgaa tgcaaccggc
      841 gcaggaacac tgccagcgca tcaacaatat tttcacctga atcaggatat tcttctaata
      901 cctggaatgc tgttttcccg gggatcgcag tggtgagtaa ccatgcatca tcaggagtac
      961 ggataaaatg cttgatggtc ggaagaggca taaattccgt cagccagttt agtctgacca
     1021 tctcatctgt aacatcattg gcaacgctac ctttgccatg tttcagaaac aactctggcg
     1081 catcgggctt cccatacaat cgatagattg tcgcacctga ttgcccgaca ttatcgcgag
     1141 cccatttata cccatataaa tcagcatcca tgttggaatt taatcgcggc ctagagcaag
     1201 acgtttcccg ttgaatatgg ctcataacac cccttgtatt actgtttatg taagcagaca
     1261 gttttattgt tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac
     1321 cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc
     1381 ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca
     1441 actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgtccttcta
     1501 gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct
     1561 ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg
     1621 gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc
     1681 acacagccca gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta
     1741 tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg
     1801 gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt
     1861 cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg
     1921 cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggt cttttgctgg
     1981 ccttttgctc acatgttctt tcctgcgtta tcccctgatt ctgtggataa ccgtattacc
     2041 gcctttgagt gagctgatac cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg
     2101 agcgaggaag cggaagagcg cctgatgcgg tattttctcc ttacgcatct gtgcggtatt
     2161 tcacaccgca atggtgcact ctcagtacaa tctgctctga tgccgcatag ttaagccagt
     2221 atacactccg ctatcgctac gtgactgggt catggctgcg ccccgacacc cgccaacacc
     2281 cgctgacgcg ccctgacggg cttgtctgct cccggcatcc gcttacagac aagctgtgac
     2341 cgtctccggg agctgcatgt gtcagaggtt ttcaccgtca tcaccgaaac gcgcgaggca
     2401 gctgcggtaa agctcatcag cgtggtcgtg aagcgattca cagatgtctg cctgttcatc
     2461 cgcgtccagc tcgttgagtt tctccagaag cgttaatgtc tggcttctga taaagcgggc
     2521 catgttaagg gcggtttttt cctgtttggt cactgatgcc tccgtgtaag ggggatttct
     2581 gttcatgggg gtaatgatac cgatgaaacg agagaggatg ctcacgatac gggttactga
     2641 tgatgaacat gcccggttac tggaacgttg tgagggtaaa caactggcgg tatggatgcg
     2701 gcgggaccag agaaaaatca ctcagggtca atgccagcgc ttcgttaata cagatgtagg
     2761 tgttccacag ggtagccagc agcatcctgc gatgcagatc cggaacataa tggtgcaggg
     2821 cgctgacttc cgcgtttcca gactttacga aacacggaaa ccgaagatca ttcatgttgt
     2881 tgctcaggtc gcagacgttt tgcagcagca gtcgcttcac gttcgctcgc gtatcggtga
     2941 ttcattctgc taaccagtaa ggcaaccccg ccagcctagc cgggtcctca acgacaggag
     3001 cacgatcatg cgcacccgtg gggccgccat gccggcgata atggcctgct tctcgccgaa
     3061 acgtttggtg gcgggaccag tgacgaaggc ttgagcgagg gcgtgcaaga ttccgaatac
     3121 cgcaagcgac aggccgatca tcgtcgcgct ccagcgaaag cggtcctcgc cgaaaatgac
     3181 ccagagcgct gccggcacct gtcctacgag ttgcatgata aagaagatag tcataagtgc
     3241 ggcgacgata gtcatgcccc gcgcccaccg gaaggagctg actgggttga aggctctcaa
     3301 gggcatcggt cgagatcccg gtgcctaatg agtgagctaa cttacattaa ttgcgttgcg
     3361 ctcactgccc gctttccagt cgggaaacct gtcgtgccag ctgcattaat gaatcggcca
     3421 acgcgcgggg agaggcggtt tgcgtattgg gcgccagggt ggtttttctt ttcaccagtg
     3481 agacgggcaa cagctgattg cccttcaccg cctggccctg agagagttgc agcaagcggt
     3541 ccacgctggt ttgccccagc aggcgaaaat cctgtttgat ggtggttaac ggcgggatat
     3601 aacatgagct atcttcggta tcgtcgtatc ccactaccga gatgtccgca ccaacgcgca
     3661 gcccggactc ggtaatggcg cgcattgcgc ccagcgccat ctgatcgttg gcaaccagca
     3721 tcgcagtggg aacgatgccc tcattcagca tttgcatggt ttgttgaaaa ccggacatgg
     3781 cactccagtc gccttcccgt tccgctatcg gctgaatttg attgcgagtg agatatttat
     3841 gccagccagc cagacgcaga cgcgccgaga cagaacttaa tgggcccgct aacagcgcga
     3901 tttgctggtg acccaatgcg accagatgct ccacgcccag tcgcgtacca tcttcatggg
     3961 agaaaataat actgttgatg ggtgtctggt cagagacatc aagaaataac gccggaacat
     4021 tagtgcaggc agcttccaca gcaatggcat cctggtcatc cagcggatag ttaatgatca
     4081 gcccactgac gcgttgcgcg agaagattgt gcaccgccgc tttacaggct tcgacgccgc
     4141 ttcgttctac catcgacacc accacgctgg cacccagttg atcggcgcga gatttaatcg
     4201 ccgcgacaat ttgcgacggc gcgtgcaggg ccagactgga ggtggcaacg ccaatcagca
     4261 acgactgttt gcccgccagt tgttgtgcca cgcggttggg aatgtaattc agctccgcca
     4321 tcgccgcttc cactttttcc cgcgttttcg cagaaacgtg gctggcctgg ttcaccacgc
     4381 gggaaacggt ctgataagag acaccggcat actctgcgac atcgtataac gttactggtt
     4441 tcacattcac caccctgaat tgactctctt ccgggcgcta tcatgccata ccgcgaaagg
     4501 ttttgcgcca ttcgatggtg tccgggatct cgacgctctc ccttatgcga ctcctgcatt
     4561 aggaagcagc ccagtagtag gttgaggccg ttgagcaccg ccgccgcaag gaatggtgca
     4621 tgcaaggaga tggcgcccaa cagtcccccg gccacggggc ctgccaccat acccacgccg
     4681 aaacaagcgc tcatgagccc gaagtggcga gcccgatctt ccccatcggt gatgtcggcg
     4741 atataggcgc cagcaaccgc acctgtggcg ccggtgatgc cggccacgat gcgtccggcg
     4801 tagaggatcg agatcgatct cgatcccgcg aaattaatac gactcactat aggggaattg
     4861 tgagcggata acaattcccc tctagaaata attttgttta actttaagaa ggagatatac
     4921 atatgtctac tccagactac tgttggcagc gtataggtct tacactaaga tgtacatttg
     4981 ctcacccagg gagaaaagcc catccacttt ctgtcctaga ggcaatagtt aaaggtattg
     5041 gagaagcagc tgggttgact gctcctacta tgagatcagt atttagatgg tccggaatga
     5101 gatcgtcgca aatgactatt gagagtggta ggatcttgtc tctcgaaatt ttgttatttg
     5161 gtactgacgc tggagctgct tgtaattggc acgaaagagc catacactac tttgatcctg
     5221 gtgcaccggg tagaaacttt caggttactg cttccgaggc ccctgtcgaa agacgatggg
     5281 cagagctatt ggctggacga caggctccag cagaatccaa tgatgagtgc tgtttggact
     5341 ttcttacccc attacccttt accccagctc aaggacgtgg aagaacatgg cttgatggtg
     5401 aaggtctacg tagagccatg caagacagat tgagaagatt atttggtgct gaggctgaac
     5461 ttccccctat tcctgaggtc ctgcctgctt attggtatta ttgtcagatt gttcacgctg
     5521 cttcctctca gccaggtcat aataaatacc ttaatggatg tttgggtcct ttgttgctta
     5581 gaggagaaca ccttggggaa tggtggcctt ggctggttct aggtgaggag attggacttg
     5641 gtggacaagt ttcttttgga caaggtttat tcagactcca tgctaagtca gttccaattc
     5701 ttgacgccag gttgacggac ccaaatcaaa ttgctgctat tattgatcag cttctattaa
     5761 gacatgatga cttagcagta agactctcta atacccctca agccccagat cttcatgagt
     5821 tggctgtgga gttggctcaa aatctcagag agggagcagc tcctctccca ttccaagcta
     5881 tcagagttcc aagatcggac ggtagactcc gtcaatttga gacacccgca gcacgagact
     5941 tagttatttt gaatcatttg acgagacttt tatcagagcc gtttgatcga ttgttttctg
     6001 tgcacagtat cggatacaga aaaggtcact ccagagagga tgcagtcgaa agagttagag
     6061 cagctattgc agagggttgt actcacgttt tggagtctga tatttctgac ttctttccgt
     6121 ctgttgactt aaaacgactc ttggcacgtc ttgatgatgt tttgccgcgt cgtgatgtaa
     6181 gattgagaca aacactcgct gcatatcttg gtgcaggatg gcgttacggt gaagggtcgg
     6241 tccaagctag aaatcgtgga cttccattag gatcaccatt gagcccactc ctagcaaacc
     6301 tttacttgga ttcatttgat tcccaactgg gagctactgt tccaggagtc aggttaatta
     6361 ggtacgctga tgattttatt atacttactg aatcagaagc agccgcaaga gctttattag
     6421 acactgctag agatgccgcc gccgctttag gtctagcttt gaatttagaa aagactgcta
     6481 ttagaccact ctcggatggt tttgacttct tgggaataag atttagtgct gacgctgctg
     6541 ctgaacaagc tggagatgaa tcagctgata gtttaagaaa agttctttat attactgagc
     6601 catacgcttt tgttggatca aatcatggta ctattgaagt gcacgccggt tccaaatcct
     6661 tgggatcatt cccactggct agaacagctg gtgtcgttac tttggtgcca tgcactctct
     6721 catctgctct aattgctcgt cttgctgatc aatgtattcc cttggctatt gcaggcacac
     6781 aaggtagaca aattgctact gtagctgggg atacggcaag gagattcgca actgctgcaa
     6841 ctcaagcaaa tagacacgct tcattaggtg aggccggtag atgtagggct gctggagcct
     6901 tcgctactgc taaacttgct aattacatag cacttataag acaaagagga ccagcaggaa
     6961 cagcagctct agtggcacgt cttgagaatg gtatagctgc tattgcttca gcaactgata
     7021 tagatgcaat tagaggtgta gaaggtgact gtgctcgtga gtgtttccca tttattgctg
     7081 gatggattaa ttctccagac ttcccttggc agggcagaag aaggcatggt gagtttcctg
     7141 acagattaaa ttctttgcta aattttggat atcatctgtt atttacgaga attaatgccc
     7201 tattgcgtgt ttccggcctg aacccttatt tgggttttct tcacgcagca aatggaaggt
     7261 atgaagccct ggcttgtgat gtacaagagg catttagacc tcatattgat aggttggtcg
     7321 taagactact aaatctcaag gttattgagg cagctgactt tgaagaatct gaggaaggat
     7381 ggtggctaat tcgtccagca cgaacaagat ttcttcagca atttgctaga gagatagagc
     7441 gacgacccat gaggcgacgt tattcccttg gtgaggctat tgagggccaa gtcagagccc
     7501 tgcacgcttg gttaattgag gatagagagt tggtcttgta tagatggtct gattcagacg
     7561 tgtaacgtac gttaacttta agaaggagaa cttaagatga gtgatatggc cctgtatatc
     7621 gtcgcctatg acataactga cgatagagaa aggcgtcaag ctgaacacat ccttcaagga
     7681 ttcgggttca gaaggcaaaa gtctgttttc gagtgcagat tgactagggg atatttagca
     7741 aggttgcagc atgagttagc tacaatcgga atggagacag ggttcgttat gatttatcac
     7801 ttagccccaa atacccgacc ttttacaata ggagaagtgc ctcacttccc agacgacgac
     7861 tgggcctggg taacttaact cgagatccgg ctgctaacaa agcccgaaag gaagctgagt
     7921 tggctgctgc caccgctgag caataactag cataacccct tggggcctct aaacgggtct
     7981 tgaggggttt tttgctgaaa ggaggaacta tatccggata
//
