LOCUS       pFS_0368_pET30-R        7339 bp    DNA     circular UNA 25-AUG-2018
DEFINITION  Concatenation of 2 sequences.
ACCESSION   urn.local...2p-9fa11gg
VERSION     urn.local...2p-9fa11gg
KEYWORDS    .
SOURCE      
  ORGANISM  
            .
FEATURES             Location/Qualifiers
     promoter        7..156
                     /created_by="User"
                     /label="Leader"
     repeat_region   157..192
                     /created_by="User"
                     /label="DR"
     misc_feature    193..197
                     /created_by="User"
                     /label="FaqI"
     promoter        379..407
                     /Imported_from="<a
                     href=""http://wishart.biology.ualberta.ca/PlasMapper"">Pla
                     sMapper</a>"
                     /Transferred_From="amp prom"
                     /Transferred_Similarity="89.66%"
                     /label="amp prom"
     marker          complement(410..1225)
                     /Imported_from="<a
                     href=""http://wishart.biology.ualberta.ca/PlasMapper"">Pla
                     sMapper</a>"
                     /Transferred_From="kan2 marker"
                     /Transferred_Similarity="99.75%"
                     /label="kan2 marker"
     misc_feature    complement(411..1225)
                     /note="KanR"
                     /label="KanR"
     misc_feature    1331..1950
                     /Imported_from="<a
                     href=""http://wishart.biology.ualberta.ca/PlasMapper"">Pla
                     sMapper</a>"
                     /Transferred_From="pBR322 origin"
                     /Transferred_Similarity="99.68%"
                     /label="pBR322 origin"
                     /note="Geneious type: origin of replication"
     gene            complement(2362..2553)
                     /Imported_from="<a
                     href=""http://wishart.biology.ualberta.ca/PlasMapper"">Pla
                     sMapper</a>"
                     /Transferred_From="ROP"
                     /Transferred_Similarity="100.00%"
                     /label="ROP"
     misc_feature    complement(3362..4453)
                     /Imported_from="<a
                     href=""http://wishart.biology.ualberta.ca/PlasMapper"">Pla
                     sMapper</a>"
                     /Transferred_From="lacI reg"
                     /Transferred_Similarity="99.91%"
                     /label="lacI reg"
                     /note="Geneious type: regulatory sequence"
     promoter        4835..4853
                     /Imported_from="<a
                     href=""http://wishart.biology.ualberta.ca/PlasMapper"">Pla
                     sMapper</a>"
                     /Transferred_From="T7 prom"
                     /Transferred_Similarity="100.00%"
                     /label="T7 prom"
     misc_feature    4853..4880
                     /Imported_from="<a
                     href=""http://wishart.biology.ualberta.ca/PlasMapper"">Pla
                     sMapper</a>"
                     /Transferred_From="lacO reg"
                     /Transferred_Similarity="100.00%"
                     /label="lacO reg"
                     /note="Geneious type: regulatory sequence"
     gene            4898..4914
                     /Imported_from="<a
                     href=""http://wishart.biology.ualberta.ca/PlasMapper"">Pla
                     sMapper</a>"
                     /Transferred_From="T7_transl_en_RBS"
                     /Transferred_Similarity="100.00%"
                     /label="T7_transl_en_RBS"
     CDS             4923..6884
                     /created_by="User"
                     /label="Cas1"
     gene            6891..6907
                     /Imported_from="<a
                     href=""http://wishart.biology.ualberta.ca/PlasMapper"">Pla
                     sMapper</a>"
                     /X-Transferred_From="T7_transl_en_RBS"
                     /X-Transferred_Similarity="100.00%"
                     /Transferred_From="Digestion of
                     pFSNW_013_pET30bvectorNovagen_Golden Gate_T7_Terminator -
                     Fragment 2, Ligated sequences"
                     /Transferred_Similarity="100.00%"
                     /label="T7_transl_en_RBS"
     CDS             6916..7197
                     /created_by="User"
                     /label="Cas2"
     misc_feature    <7199..>7202
                     /note="XhoI"
                     /label="XhoI"
     terminator      7210..7338
                     /created_by="User"
                     /label="Terminator extended"
     terminator      7271..7314
                     /Imported_from="<a
                     href=""http://wishart.biology.ualberta.ca/PlasMapper"">Pla
                     sMapper</a>"
                     /Transferred_From="T7 term"
                     /Transferred_Similarity="97.73%"
                     /label="T7 term"
     misc_feature    complement(7339..>5)
                     /label="CsiI"
ORIGIN      
        1 ccaggtgcac gcagcgcagc gcctttcggg ttggccccgc ttatgaacat cggtgtgatg
       61 atcttcaatg tcaggttcag ttgctgcatc ttttccccat cccggcgtgc acgttaaatg
      121 tgcattttgc ggctttcaag ggaaaattaa cgcgaagttt tgatcagctc tgctgaaaga
      181 ttcgagcaga gcgtcccgcg gccgctattc ttttgattta taagggattt tgccgatttc
      241 ggcctattgg ttaaaaaatg agctgattta acaaaaattt aacgcgaatt ttaacaaaat
      301 attaacgctt acaatttcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg
      361 tttatttttc taaatacatt caaatatgta tccgctcatg aattaattct tagaaaaact
      421 catcgagcat caaatgaaac tgcaatttat tcatatcagg attatcaata ccatattttt
      481 gaaaaagccg tttctgtaat gaaggagaaa actcaccgag gcagttccat aggatggcaa
      541 gatcctggta tcggtctgcg attccgactc gtccaacatc aatacaacct attaatttcc
      601 cctcgtcaaa aataaggtta tcaagtgaga aatcaccatg agtgacgact gaatccggtg
      661 agaatggcaa aagtttatgc atttctttcc agacttgttc aacaggccag ccattacgct
      721 cgtcatcaaa atcactcgca tcaaccaaac cgttattcat tcgtgattgc gcctgagcga
      781 gacgaaatac gcgatcgctg ttaaaaggac aattacaaac aggaatcgaa tgcaaccggc
      841 gcaggaacac tgccagcgca tcaacaatat tttcacctga atcaggatat tcttctaata
      901 cctggaatgc tgttttcccg gggatcgcag tggtgagtaa ccatgcatca tcaggagtac
      961 ggataaaatg cttgatggtc ggaagaggca taaattccgt cagccagttt agtctgacca
     1021 tctcatctgt aacatcattg gcaacgctac ctttgccatg tttcagaaac aactctggcg
     1081 catcgggctt cccatacaat cgatagattg tcgcacctga ttgcccgaca ttatcgcgag
     1141 cccatttata cccatataaa tcagcatcca tgttggaatt taatcgcggc ctagagcaag
     1201 acgtttcccg ttgaatatgg ctcataacac cccttgtatt actgtttatg taagcagaca
     1261 gttttattgt tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac
     1321 cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc
     1381 ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca
     1441 actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgtccttcta
     1501 gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct
     1561 ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg
     1621 gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc
     1681 acacagccca gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta
     1741 tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg
     1801 gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt
     1861 cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg
     1921 cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggt cttttgctgg
     1981 ccttttgctc acatgttctt tcctgcgtta tcccctgatt ctgtggataa ccgtattacc
     2041 gcctttgagt gagctgatac cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg
     2101 agcgaggaag cggaagagcg cctgatgcgg tattttctcc ttacgcatct gtgcggtatt
     2161 tcacaccgca atggtgcact ctcagtacaa tctgctctga tgccgcatag ttaagccagt
     2221 atacactccg ctatcgctac gtgactgggt catggctgcg ccccgacacc cgccaacacc
     2281 cgctgacgcg ccctgacggg cttgtctgct cccggcatcc gcttacagac aagctgtgac
     2341 cgtctccggg agctgcatgt gtcagaggtt ttcaccgtca tcaccgaaac gcgcgaggca
     2401 gctgcggtaa agctcatcag cgtggtcgtg aagcgattca cagatgtctg cctgttcatc
     2461 cgcgtccagc tcgttgagtt tctccagaag cgttaatgtc tggcttctga taaagcgggc
     2521 catgttaagg gcggtttttt cctgtttggt cactgatgcc tccgtgtaag ggggatttct
     2581 gttcatgggg gtaatgatac cgatgaaacg agagaggatg ctcacgatac gggttactga
     2641 tgatgaacat gcccggttac tggaacgttg tgagggtaaa caactggcgg tatggatgcg
     2701 gcgggaccag agaaaaatca ctcagggtca atgccagcgc ttcgttaata cagatgtagg
     2761 tgttccacag ggtagccagc agcatcctgc gatgcagatc cggaacataa tggtgcaggg
     2821 cgctgacttc cgcgtttcca gactttacga aacacggaaa ccgaagatca ttcatgttgt
     2881 tgctcaggtc gcagacgttt tgcagcagca gtcgcttcac gttcgctcgc gtatcggtga
     2941 ttcattctgc taaccagtaa ggcaaccccg ccagcctagc cgggtcctca acgacaggag
     3001 cacgatcatg cgcacccgtg gggccgccat gccggcgata atggcctgct tctcgccgaa
     3061 acgtttggtg gcgggaccag tgacgaaggc ttgagcgagg gcgtgcaaga ttccgaatac
     3121 cgcaagcgac aggccgatca tcgtcgcgct ccagcgaaag cggtcctcgc cgaaaatgac
     3181 ccagagcgct gccggcacct gtcctacgag ttgcatgata aagaagatag tcataagtgc
     3241 ggcgacgata gtcatgcccc gcgcccaccg gaaggagctg actgggttga aggctctcaa
     3301 gggcatcggt cgagatcccg gtgcctaatg agtgagctaa cttacattaa ttgcgttgcg
     3361 ctcactgccc gctttccagt cgggaaacct gtcgtgccag ctgcattaat gaatcggcca
     3421 acgcgcgggg agaggcggtt tgcgtattgg gcgccagggt ggtttttctt ttcaccagtg
     3481 agacgggcaa cagctgattg cccttcaccg cctggccctg agagagttgc agcaagcggt
     3541 ccacgctggt ttgccccagc aggcgaaaat cctgtttgat ggtggttaac ggcgggatat
     3601 aacatgagct atcttcggta tcgtcgtatc ccactaccga gatgtccgca ccaacgcgca
     3661 gcccggactc ggtaatggcg cgcattgcgc ccagcgccat ctgatcgttg gcaaccagca
     3721 tcgcagtggg aacgatgccc tcattcagca tttgcatggt ttgttgaaaa ccggacatgg
     3781 cactccagtc gccttcccgt tccgctatcg gctgaatttg attgcgagtg agatatttat
     3841 gccagccagc cagacgcaga cgcgccgaga cagaacttaa tgggcccgct aacagcgcga
     3901 tttgctggtg acccaatgcg accagatgct ccacgcccag tcgcgtacca tcttcatggg
     3961 agaaaataat actgttgatg ggtgtctggt cagagacatc aagaaataac gccggaacat
     4021 tagtgcaggc agcttccaca gcaatggcat cctggtcatc cagcggatag ttaatgatca
     4081 gcccactgac gcgttgcgcg agaagattgt gcaccgccgc tttacaggct tcgacgccgc
     4141 ttcgttctac catcgacacc accacgctgg cacccagttg atcggcgcga gatttaatcg
     4201 ccgcgacaat ttgcgacggc gcgtgcaggg ccagactgga ggtggcaacg ccaatcagca
     4261 acgactgttt gcccgccagt tgttgtgcca cgcggttggg aatgtaattc agctccgcca
     4321 tcgccgcttc cactttttcc cgcgttttcg cagaaacgtg gctggcctgg ttcaccacgc
     4381 gggaaacggt ctgataagag acaccggcat actctgcgac atcgtataac gttactggtt
     4441 tcacattcac caccctgaat tgactctctt ccgggcgcta tcatgccata ccgcgaaagg
     4501 ttttgcgcca ttcgatggtg tccgggatct cgacgctctc ccttatgcga ctcctgcatt
     4561 aggaagcagc ccagtagtag gttgaggccg ttgagcaccg ccgccgcaag gaatggtgca
     4621 tgcaaggaga tggcgcccaa cagtcccccg gccacggggc ctgccaccat acccacgccg
     4681 aaacaagcgc tcatgagccc gaagtggcga gcccgatctt ccccatcggt gatgtcggcg
     4741 atataggcgc cagcaaccgc acctgtggcg ccggtgatgc cggccacgat gcgtccggcg
     4801 tagaggatcg agatcgatct cgatcccgcg aaattaatac gactcactat aggggaattg
     4861 tgagcggata acaattcccc tctagaaata attttgttta actttaagaa ggagatatac
     4921 atatgatgag attagaggca tgtttaactg ttaagtccct tatgacagct tggaagaaag
     4981 tatacgcaaa ccaaggtggt ccaggtggag atggacagac tctagcacag tttcagagaa
     5041 ctgttctctt acaccttcac agattgggtg acgatgttcg agcaggacta tacatgcctg
     5101 gacctcacag ggttgtttcg atcccaaaac gagccggtgg atggagatca ctttctattc
     5161 cgtgtgttcg agatcgagtt ttgcagactg ctgttgctca aaggttacag ccgatactcg
     5221 aacctgaatt tgagccagaa agctacggat accgtccagg tagatctgtt gctcaagcta
     5281 ttgctagggt ggctactctt agaaggcaag gcttcagatg gactgttgac gctgacatag
     5341 aacgtttctt cgattgcgtc ccgcacggac cactattgga aagacttagg ccctttcttg
     5401 gcgacccagg tttagtaggt ctagtcgaaa tgtggttggc tggagctggg ccccacggca
     5461 gaggattgcc tcagggtagt ccaattagtc cactccttgc taacttgtac ttggacgacg
     5521 tagacgaagg tctaaagtca acacacacta gattggttag atttgctgac gactttgtta
     5581 tcttgactcg taacgaggac gaagctctac aagccctaga acgagctagg ggtttgctag
     5641 acaaactggg tctttcattg aatttggaga aaactaggat tgtaccattc gagggtggtc
     5701 ttgatttctt gggtagaaag ttcgtaaggg ctcttgttgt ggacgacgtt tacgaggacg
     5761 agggtgaagc cctagctgct ggcgagccag ctcgtaccca tgccagagct atgcctggtg
     5821 aagatgcttt agacttggct tcccttgagg caggcgctga taccagtaga gcacctagac
     5881 taagagtttt gtatctgtta gagaagggac acgtcttagg tactcacaat agttcattta
     5941 ctgtcagaaa cgctcaaggt gacccagtga gtacccttcc gacaacgaga gtagatagaa
     6001 tcgaagtagg ttctcaagct agaattgctg acgaagctat caggttggcc cttgacgagg
     6061 atgtcgagat gaggtggatt aacggtcgag gtcagactga gggatacttg tcacgtcccg
     6121 agagaggaca cggtgcttta catttggctc aacttagact gtacgataat gcagaagcta
     6181 ggcttgcagc tgctagaatc ttagtcgaag gtagattgcg taaccaaaga gctttactga
     6241 gaaggctcaa tagaagaaga aaacgaccct ttatcgctga aagggctaaa caaatcggtg
     6301 gtgttttaaa gtatctccct gaggcccaga ctatcgaagc tctgatgggt agggaaggcc
     6361 aagctggtgc cttgtattgg ccagctttgg gtgcttgttt ggaacacgga tggactttta
     6421 cccaaagggt aaggcgaccg cccccagacc ctgtaaactt ggtactgtcg tacttggctt
     6481 ctttgctgtg cagggacata gcttccttgg ccgccagaca agggctccac gtaggtattg
     6541 gcgctctaca tgcagttcag gatgaaccga gggatacttt agcattcgac ctcgctgagg
     6601 agttcagagc accgctcgtt gaaggacttt gcatttggat gttaaacaca cgtacattgg
     6661 gtcaccagat gttccatact agagaggacg gacaagttta cgcacacgga gaaggaatta
     6721 aaacaatact gagatcctgg gaaaactggt tggatagacc agtaagatct cctagatctg
     6781 gccaggatgt cttgtggaga gggcttattg aagaacagat cttgtgttgg agagatcacg
     6841 tgagtggaaa atccgtgtac caaccgtaca gaatggagta ctaacgtacg ttaactttaa
     6901 gaaggagaac ttaagatggc agatccattg tttgttttcg catacgacat ctcacaggac
     6961 cgtgtgagaa ggagagtcgc aggactactt gaagcagaag ccgtaagagt acaaggtagc
     7021 gtctttgaag ctagattgtc caaaagtaga gctaaggctc tgggacaaag aattgctata
     7081 gaattagaaa ggggagacac cttgagagtc tactgcttgt ctcagaggga cctcaagagt
     7141 tctttccagt ggggtggcgc tccaatggct gaggcacaag acttttatat tctgtaactc
     7201 gagatccggc tgctaacaaa gcccgaaagg aagctgagtt ggctgctgcc accgctgagc
     7261 aataactagc ataacccctt ggggcctcta aacgggtctt gaggggtttt ttgctgaaag
     7321 gaggaactat atccggata
//
