LOCUS       Exported                7403 bp ds-DNA     circular UNA 25-AUG-2018
DEFINITION  Concatenation of 2 sequences.
ACCESSION   urn.local...2r-9fa11gg
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 7403)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Aug 29, 2018 from SnapGene Viewer 4.2.4
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..7403
                     /organism="unspecified"
                     /mol_type="genomic DNA"
     promoter        7..156
                     /label=Leader
                     /note="/created_by=User"
     repeat_region   157..193
                     /label=DR
                     /note="/created_by=User"
     misc_feature    194..198
                     /label=FaqI
                     /note="/created_by=User"
     promoter        380..408
                     /label=amp prom
                     /note="/Imported_from=Pla sMapper"
                     /note="/Transferred_From=amp prom"
                     /note="/Transferred_Similarity=89.66%"
     misc_feature    complement(411..1226)
                     /label=kan2 marker
                     /label=nonstandard type: marker
                     /note="/Imported_from=Pla sMapper"
                     /note="/Transferred_From=kan2 marker"
                     /note="/Transferred_Similarity=99.75%"
     misc_feature    complement(412..1226)
                     /label=KanR
                     /note="KanR"
     misc_feature    1332..1951
                     /label=pBR322 origin
                     /note="Geneious type: origin of replication"
                     /note="/Imported_from=Pla sMapper"
                     /note="/Transferred_From=pBR322 origin"
                     /note="/Transferred_Similarity=99.68%"
     gene            complement(2363..2554)
                     /label=ROP
                     /note="/Imported_from=Pla sMapper"
                     /note="/Transferred_From=ROP"
                     /note="/Transferred_Similarity=100.00%"
     misc_feature    complement(3363..4454)
                     /label=lacI reg
                     /note="Geneious type: regulatory sequence"
                     /note="/Imported_from=Pla sMapper"
                     /note="/Transferred_From=lacI reg"
                     /note="/Transferred_Similarity=99.91%"
     promoter        4836..4854
                     /label=T7 prom
                     /note="/Imported_from=Pla sMapper"
                     /note="/Transferred_From=T7 prom"
                     /note="/Transferred_Similarity=100.00%"
     misc_feature    4854..4881
                     /label=lacO reg
                     /note="Geneious type: regulatory sequence"
                     /note="/Imported_from=Pla sMapper"
                     /note="/Transferred_From=lacO reg"
                     /note="/Transferred_Similarity=100.00%"
     gene            4899..4915
                     /label=T7_transl_en_RBS
                     /note="/Imported_from=Pla sMapper"
                     /note="/Transferred_From=T7_transl_en_RBS"
                     /note="/Transferred_Similarity=100.00%"
     CDS             4924..6942
                     /label=Cas1
                     /note="/created_by=User"
     gene            6949..6965
                     /label=T7_transl_en_RBS
                     /note="/Imported_from=Pla sMapper"
                     /note="/Transferred_From=Digestion of 
                     pFSNW_013_pET30bvectorNovagen_Golden Gate_T7_Terminator - 
                     Fragment 2, Ligated sequences, Ligated sequences 2"
                     /note="/Transferred_Similarity=100.00%"
                     /note="/X-Transferred_From=T7_transl_en_RBS"
                     /note="/X-Transferred_Similarity=100.00%"
     CDS             6974..7261
                     /label=Cas2
                     /note="/created_by=User"
     misc_feature    7263..7266
                     /label=XhoI
                     /note="XhoI"
     terminator      7274..7402
                     /label=Terminator extended
                     /note="/created_by=User"
     terminator      7335..7378
                     /label=T7 term
                     /note="/Imported_from=Pla sMapper"
                     /note="/Transferred_From=T7 term"
                     /note="/Transferred_Similarity=97.73%"
     misc_feature    complement(join(7403,1..5))
                     /label=CsiI
ORIGIN
        1 ccaggttcgg cgcacgggtt ggcgcacagc gccgcgctgg gcggcgcccc ggtgccggaa
       61 cagcaggatt tctggttgct gtagggggga ggaagccggc cgcgacaatt ggctctcggc
      121 cggggggatc atcttcctcg acgaaatcac gcgagcgttt caatccgctc ccgaacgcgg
      181 atgccgtcgc gacgtcccgc ggccgctatt cttttgattt ataagggatt ttgccgattt
      241 cggcctattg gttaaaaaat gagctgattt aacaaaaatt taacgcgaat tttaacaaaa
      301 tattaacgct tacaatttca ggtggcactt ttcggggaaa tgtgcgcgga acccctattt
      361 gtttattttt ctaaatacat tcaaatatgt atccgctcat gaattaattc ttagaaaaac
      421 tcatcgagca tcaaatgaaa ctgcaattta ttcatatcag gattatcaat accatatttt
      481 tgaaaaagcc gtttctgtaa tgaaggagaa aactcaccga ggcagttcca taggatggca
      541 agatcctggt atcggtctgc gattccgact cgtccaacat caatacaacc tattaatttc
      601 ccctcgtcaa aaataaggtt atcaagtgag aaatcaccat gagtgacgac tgaatccggt
      661 gagaatggca aaagtttatg catttctttc cagacttgtt caacaggcca gccattacgc
      721 tcgtcatcaa aatcactcgc atcaaccaaa ccgttattca ttcgtgattg cgcctgagcg
      781 agacgaaata cgcgatcgct gttaaaagga caattacaaa caggaatcga atgcaaccgg
      841 cgcaggaaca ctgccagcgc atcaacaata ttttcacctg aatcaggata ttcttctaat
      901 acctggaatg ctgttttccc ggggatcgca gtggtgagta accatgcatc atcaggagta
      961 cggataaaat gcttgatggt cggaagaggc ataaattccg tcagccagtt tagtctgacc
     1021 atctcatctg taacatcatt ggcaacgcta cctttgccat gtttcagaaa caactctggc
     1081 gcatcgggct tcccatacaa tcgatagatt gtcgcacctg attgcccgac attatcgcga
     1141 gcccatttat acccatataa atcagcatcc atgttggaat ttaatcgcgg cctagagcaa
     1201 gacgtttccc gttgaatatg gctcataaca ccccttgtat tactgtttat gtaagcagac
     1261 agttttattg ttcatgacca aaatccctta acgtgagttt tcgttccact gagcgtcaga
     1321 ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg
     1381 cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc
     1441 aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgtccttct
     1501 agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc
     1561 tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt
     1621 ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg
     1681 cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagct
     1741 atgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg taagcggcag
     1801 ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag
     1861 tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg
     1921 gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg tcttttgctg
     1981 gccttttgct cacatgttct ttcctgcgtt atcccctgat tctgtggata accgtattac
     2041 cgcctttgag tgagctgata ccgctcgccg cagccgaacg accgagcgca gcgagtcagt
     2101 gagcgaggaa gcggaagagc gcctgatgcg gtattttctc cttacgcatc tgtgcggtat
     2161 ttcacaccgc aatggtgcac tctcagtaca atctgctctg atgccgcata gttaagccag
     2221 tatacactcc gctatcgcta cgtgactggg tcatggctgc gccccgacac ccgccaacac
     2281 ccgctgacgc gccctgacgg gcttgtctgc tcccggcatc cgcttacaga caagctgtga
     2341 ccgtctccgg gagctgcatg tgtcagaggt tttcaccgtc atcaccgaaa cgcgcgaggc
     2401 agctgcggta aagctcatca gcgtggtcgt gaagcgattc acagatgtct gcctgttcat
     2461 ccgcgtccag ctcgttgagt ttctccagaa gcgttaatgt ctggcttctg ataaagcggg
     2521 ccatgttaag ggcggttttt tcctgtttgg tcactgatgc ctccgtgtaa gggggatttc
     2581 tgttcatggg ggtaatgata ccgatgaaac gagagaggat gctcacgata cgggttactg
     2641 atgatgaaca tgcccggtta ctggaacgtt gtgagggtaa acaactggcg gtatggatgc
     2701 ggcgggacca gagaaaaatc actcagggtc aatgccagcg cttcgttaat acagatgtag
     2761 gtgttccaca gggtagccag cagcatcctg cgatgcagat ccggaacata atggtgcagg
     2821 gcgctgactt ccgcgtttcc agactttacg aaacacggaa accgaagatc attcatgttg
     2881 ttgctcaggt cgcagacgtt ttgcagcagc agtcgcttca cgttcgctcg cgtatcggtg
     2941 attcattctg ctaaccagta aggcaacccc gccagcctag ccgggtcctc aacgacagga
     3001 gcacgatcat gcgcacccgt ggggccgcca tgccggcgat aatggcctgc ttctcgccga
     3061 aacgtttggt ggcgggacca gtgacgaagg cttgagcgag ggcgtgcaag attccgaata
     3121 ccgcaagcga caggccgatc atcgtcgcgc tccagcgaaa gcggtcctcg ccgaaaatga
     3181 cccagagcgc tgccggcacc tgtcctacga gttgcatgat aaagaagata gtcataagtg
     3241 cggcgacgat agtcatgccc cgcgcccacc ggaaggagct gactgggttg aaggctctca
     3301 agggcatcgg tcgagatccc ggtgcctaat gagtgagcta acttacatta attgcgttgc
     3361 gctcactgcc cgctttccag tcgggaaacc tgtcgtgcca gctgcattaa tgaatcggcc
     3421 aacgcgcggg gagaggcggt ttgcgtattg ggcgccaggg tggtttttct tttcaccagt
     3481 gagacgggca acagctgatt gcccttcacc gcctggccct gagagagttg cagcaagcgg
     3541 tccacgctgg tttgccccag caggcgaaaa tcctgtttga tggtggttaa cggcgggata
     3601 taacatgagc tatcttcggt atcgtcgtat cccactaccg agatgtccgc accaacgcgc
     3661 agcccggact cggtaatggc gcgcattgcg cccagcgcca tctgatcgtt ggcaaccagc
     3721 atcgcagtgg gaacgatgcc ctcattcagc atttgcatgg tttgttgaaa accggacatg
     3781 gcactccagt cgccttcccg ttccgctatc ggctgaattt gattgcgagt gagatattta
     3841 tgccagccag ccagacgcag acgcgccgag acagaactta atgggcccgc taacagcgcg
     3901 atttgctggt gacccaatgc gaccagatgc tccacgccca gtcgcgtacc atcttcatgg
     3961 gagaaaataa tactgttgat gggtgtctgg tcagagacat caagaaataa cgccggaaca
     4021 ttagtgcagg cagcttccac agcaatggca tcctggtcat ccagcggata gttaatgatc
     4081 agcccactga cgcgttgcgc gagaagattg tgcaccgccg ctttacaggc ttcgacgccg
     4141 cttcgttcta ccatcgacac caccacgctg gcacccagtt gatcggcgcg agatttaatc
     4201 gccgcgacaa tttgcgacgg cgcgtgcagg gccagactgg aggtggcaac gccaatcagc
     4261 aacgactgtt tgcccgccag ttgttgtgcc acgcggttgg gaatgtaatt cagctccgcc
     4321 atcgccgctt ccactttttc ccgcgttttc gcagaaacgt ggctggcctg gttcaccacg
     4381 cgggaaacgg tctgataaga gacaccggca tactctgcga catcgtataa cgttactggt
     4441 ttcacattca ccaccctgaa ttgactctct tccgggcgct atcatgccat accgcgaaag
     4501 gttttgcgcc attcgatggt gtccgggatc tcgacgctct cccttatgcg actcctgcat
     4561 taggaagcag cccagtagta ggttgaggcc gttgagcacc gccgccgcaa ggaatggtgc
     4621 atgcaaggag atggcgccca acagtccccc ggccacgggg cctgccacca tacccacgcc
     4681 gaaacaagcg ctcatgagcc cgaagtggcg agcccgatct tccccatcgg tgatgtcggc
     4741 gatataggcg ccagcaaccg cacctgtggc gccggtgatg ccggccacga tgcgtccggc
     4801 gtagaggatc gagatcgatc tcgatcccgc gaaattaata cgactcacta taggggaatt
     4861 gtgagcggat aacaattccc ctctagaaat aattttgttt aactttaaga aggagatata
     4921 catatgccta cggcaccccc tgataatgca gacctatttg aagaagtgac cagattggac
     4981 acacttgaga gagcttgggg tagagtcttg cgaaatgctg gtgctgctgg tggtgacggt
     5041 ttgactgtgg gtcgttttgc agaagctgct ccatctaggc ttttggcatt acatcgtact
     5101 cttagaatgg gtgactatag gcctggtccc cttagaagat tatctattcc aaaaccagat
     5161 ggtgctttaa gaccactggc tatcccacca gtcactgatc gtgtcgctca aactgctgct
     5221 gctcttgtat tgacgccact tttggacgga gaatttgagg acgcttcatt tgggtatcga
     5281 cctggcagat cagttcctca agcagtagcc agggtagcta gatggagaga tcaaggatac
     5341 gattgggtcg tggatgcaga tattgagaga tactttgagc gtgttccaca tgatagactt
     5401 ctaatacgat tggaaaggag tattggtgct ggtccactta ctgaattgat tgcagtgtgg
     5461 ttagagtcgg gagccgaaaa tggtgttggt ttacctcaag gttctccatt aagtccatta
     5521 ctatctaatt tgtatcttga tgacttagat gaagctcttg atggcagagg tctacgtctt
     5581 gttaggtttg cagatgactt tgttctattg tgtagaagca gagaacgtgc tgagagagct
     5641 ttagatcacg cagctgctgt attggaagaa cacggtttga gactgaatag agataaaacc
     5701 agaattgttc cctttgatca aggatttagg ttcttgggtc acctatttgt aagatccttg
     5761 gttttgcctt caccaagacc agacgatggt gaagaaaccg aaggtgacgc attattgaga
     5821 gcactcgcaa ttagagatgc cgccgctgaa gcagaagaag ctgccgcaga agaaagagat
     5881 aggcaaagtc gtgctgctgg gcttgatcct gcacttagaa cgttacacgt acaaacgcct
     5941 agaaggagac tagctttaag gaatgaggct tttactgttg ttgagaggga tgaccttggt
     6001 ggtgaaagag aactcattgc tattcatcat catcatttgg atcgtattga tctaggccca
     6061 gaagcagatg cagacgctga agcattgaga tgggcccttg ctacagatac tgagttggca
     6121 tttgttgatg gccacggtgc tactcacggt agactaacga ggcctgaagc taggagagct
     6181 gctttgcatt tagaccaagc ccgtcatgct cttgatgaag gtttgcgttt agatctagca
     6241 agaagaattg tagatggaag attgcgaaat caaagagcac ttcttagaag attgaatcgt
     6301 tccagaaaac ttggagttgt cgaggacgct gtcctcgcta taaatgcact acttagaaga
     6361 ttagatacag ctgctgatgt tgctgcattg ttaggatttg aaggtcaggg agctgctcga
     6421 tactggccag ctttagctgc ccaaattgaa ggagagtggg aatttgaagg aagaaggcgt
     6481 agaccacccc ctgaccctgt aaatgctgta ctctcttacc tctcgggttt attggagaga
     6541 gatgtagctg ctcttattgc taggcatggg ctacatccgg gatttggagt actacattca
     6601 ccacaagatc gacatgatgc aggaatttat gatttgatgg aagaatttag agccccctta
     6661 atggaaggcc tggctgttac tttgtttaat aggagaacgc tcaggccaga tcactttagt
     6721 agacgtgaaa cgggtgaggg agaaattaaa ggatgtcgta tcgaccccga tgctgttgga
     6781 gctattatta gggcttacga gcaatgggtc cgtcgtcctg ttgcatctcc aagagatggt
     6841 aaaagaacta cttggagagg tcttatagga ttccaagcac aagcattggc tgcacacgtc
     6901 caaggaaggg aaccctatag agcttacgtg atggactatt agcgtacgtt aactttaaga
     6961 aggagaactt aagatgtctg aaacaccaat ggttatggtg ttctgctacg atgtcgccaa
     7021 tgatagaaga agaaggagag tctcagcagt acttgaggag tggggagtta gggtacaaaa
     7081 gtccgtattt gaagcaagac taactgagcc tcaagctaga gctttgttag caagagctgg
     7141 aaaagaactg ggtccaagag attctcttag aatgtatgca cttagcgcac atggcctcgc
     7201 tcattctgct gccctcggtg gggctccagt tcccgagcaa caagactttt ggctcttgta
     7261 actcgagatc cggctgctaa caaagcccga aaggaagctg agttggctgc tgccaccgct
     7321 gagcaataac tagcataacc ccttggggcc tctaaacggg tcttgagggg ttttttgctg
     7381 aaaggaggaa ctatatccgg ata
//
