LOCUS       pFS_0375_pET30-R        7380 bp    DNA     circular UNA 25-AUG-2018
DEFINITION  Concatenation of 2 sequences.
ACCESSION   urn.local...2w-9fa11gh
VERSION     urn.local...2w-9fa11gh
KEYWORDS    .
SOURCE      
  ORGANISM  
            .
FEATURES             Location/Qualifiers
     promoter        7..156
                     /created_by="User"
                     /label="Leader"
     repeat_region   157..191
                     /created_by="User"
                     /label="DR"
     misc_feature    192..196
                     /created_by="User"
                     /label="FaqI"
     promoter        378..406
                     /Imported_from="<a
                     href=""http://wishart.biology.ualberta.ca/PlasMapper"">Pla
                     sMapper</a>"
                     /Transferred_From="amp prom"
                     /Transferred_Similarity="89.66%"
                     /label="amp prom"
     marker          complement(409..1224)
                     /Imported_from="<a
                     href=""http://wishart.biology.ualberta.ca/PlasMapper"">Pla
                     sMapper</a>"
                     /Transferred_From="kan2 marker"
                     /Transferred_Similarity="99.75%"
                     /label="kan2 marker"
     misc_feature    complement(410..1224)
                     /note="KanR"
                     /label="KanR"
     misc_feature    1330..1949
                     /Imported_from="<a
                     href=""http://wishart.biology.ualberta.ca/PlasMapper"">Pla
                     sMapper</a>"
                     /Transferred_From="pBR322 origin"
                     /Transferred_Similarity="99.68%"
                     /label="pBR322 origin"
                     /note="Geneious type: origin of replication"
     gene            complement(2361..2552)
                     /Imported_from="<a
                     href=""http://wishart.biology.ualberta.ca/PlasMapper"">Pla
                     sMapper</a>"
                     /Transferred_From="ROP"
                     /Transferred_Similarity="100.00%"
                     /label="ROP"
     misc_feature    complement(3361..4452)
                     /Imported_from="<a
                     href=""http://wishart.biology.ualberta.ca/PlasMapper"">Pla
                     sMapper</a>"
                     /Transferred_From="lacI reg"
                     /Transferred_Similarity="99.91%"
                     /label="lacI reg"
                     /note="Geneious type: regulatory sequence"
     promoter        4834..4852
                     /Imported_from="<a
                     href=""http://wishart.biology.ualberta.ca/PlasMapper"">Pla
                     sMapper</a>"
                     /Transferred_From="T7 prom"
                     /Transferred_Similarity="100.00%"
                     /label="T7 prom"
     misc_feature    4852..4879
                     /Imported_from="<a
                     href=""http://wishart.biology.ualberta.ca/PlasMapper"">Pla
                     sMapper</a>"
                     /Transferred_From="lacO reg"
                     /Transferred_Similarity="100.00%"
                     /label="lacO reg"
                     /note="Geneious type: regulatory sequence"
     gene            4897..4913
                     /Imported_from="<a
                     href=""http://wishart.biology.ualberta.ca/PlasMapper"">Pla
                     sMapper</a>"
                     /Transferred_From="T7_transl_en_RBS"
                     /Transferred_Similarity="100.00%"
                     /label="T7_transl_en_RBS"
     misc_feature    4919..4924
                     /Recognition_pattern="CA^TATG"
                     /annotation_group="CATATG"
                     /label="NdeI"
                     /note="Geneious type: restriction site"
     CDS             4922..6928
                     /created_by="User"
                     /label="Cas1"
     gene            6935..6951
                     /Imported_from="<a
                     href=""http://wishart.biology.ualberta.ca/PlasMapper"">Pla
                     sMapper</a>"
                     /X-Transferred_From="T7_transl_en_RBS"
                     /X-Transferred_Similarity="100.00%"
                     /Transferred_From="Digestion of
                     pFSNW_013_pET30bvectorNovagen_Golden Gate_T7_Terminator -
                     Fragment 2, Ligated sequences"
                     /Transferred_Similarity="100.00%"
                     /label="T7_transl_en_RBS"
     misc_feature    6954..6959
                     /Recognition_pattern="C^TTAAG"
                     /annotation_group="CTTAAG"
                     /label="AflII"
                     /note="Geneious type: restriction site"
     CDS             6960..7238
                     /created_by="User"
                     /label="Cas2"
     misc_feature    <7240..>7243
                     /note="XhoI"
                     /label="XhoI"
     terminator      7251..7379
                     /created_by="User"
                     /label="Terminator extended"
     terminator      7312..7355
                     /Imported_from="<a
                     href=""http://wishart.biology.ualberta.ca/PlasMapper"">Pla
                     sMapper</a>"
                     /Transferred_From="T7 term"
                     /Transferred_Similarity="97.73%"
                     /label="T7 term"
     misc_feature    complement(7380..>5)
                     /label="CsiI"
ORIGIN      
        1 ccaggtttct atcccacaaa tcagcttcct cacaaaggtt tgaggtactt tggctggata
       61 gttgttgaga gtaattcgca tttatttgga cgaaaatttg catcccacaa aaaagcgctg
      121 tagaattatt tcagggtaag gcttctagga gctggtcttt aaacttcttc ggaagttgaa
      181 ttaatggaaa cgtcccgcgg ccgctattct tttgatttat aagggatttt gccgatttcg
      241 gcctattggt taaaaaatga gctgatttaa caaaaattta acgcgaattt taacaaaata
      301 ttaacgctta caatttcagg tggcactttt cggggaaatg tgcgcggaac ccctatttgt
      361 ttatttttct aaatacattc aaatatgtat ccgctcatga attaattctt agaaaaactc
      421 atcgagcatc aaatgaaact gcaatttatt catatcagga ttatcaatac catatttttg
      481 aaaaagccgt ttctgtaatg aaggagaaaa ctcaccgagg cagttccata ggatggcaag
      541 atcctggtat cggtctgcga ttccgactcg tccaacatca atacaaccta ttaatttccc
      601 ctcgtcaaaa ataaggttat caagtgagaa atcaccatga gtgacgactg aatccggtga
      661 gaatggcaaa agtttatgca tttctttcca gacttgttca acaggccagc cattacgctc
      721 gtcatcaaaa tcactcgcat caaccaaacc gttattcatt cgtgattgcg cctgagcgag
      781 acgaaatacg cgatcgctgt taaaaggaca attacaaaca ggaatcgaat gcaaccggcg
      841 caggaacact gccagcgcat caacaatatt ttcacctgaa tcaggatatt cttctaatac
      901 ctggaatgct gttttcccgg ggatcgcagt ggtgagtaac catgcatcat caggagtacg
      961 gataaaatgc ttgatggtcg gaagaggcat aaattccgtc agccagttta gtctgaccat
     1021 ctcatctgta acatcattgg caacgctacc tttgccatgt ttcagaaaca actctggcgc
     1081 atcgggcttc ccatacaatc gatagattgt cgcacctgat tgcccgacat tatcgcgagc
     1141 ccatttatac ccatataaat cagcatccat gttggaattt aatcgcggcc tagagcaaga
     1201 cgtttcccgt tgaatatggc tcataacacc ccttgtatta ctgtttatgt aagcagacag
     1261 ttttattgtt catgaccaaa atcccttaac gtgagttttc gttccactga gcgtcagacc
     1321 ccgtagaaaa gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct
     1381 tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa
     1441 ctctttttcc gaaggtaact ggcttcagca gagcgcagat accaaatact gtccttctag
     1501 tgtagccgta gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc
     1561 tgctaatcct gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg
     1621 actcaagacg atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca
     1681 cacagcccag cttggagcga acgacctaca ccgaactgag atacctacag cgtgagctat
     1741 gagaaagcgc cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg
     1801 tcggaacagg agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc
     1861 ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc
     1921 ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg gttcctggtc ttttgctggc
     1981 cttttgctca catgttcttt cctgcgttat cccctgattc tgtggataac cgtattaccg
     2041 cctttgagtg agctgatacc gctcgccgca gccgaacgac cgagcgcagc gagtcagtga
     2101 gcgaggaagc ggaagagcgc ctgatgcggt attttctcct tacgcatctg tgcggtattt
     2161 cacaccgcaa tggtgcactc tcagtacaat ctgctctgat gccgcatagt taagccagta
     2221 tacactccgc tatcgctacg tgactgggtc atggctgcgc cccgacaccc gccaacaccc
     2281 gctgacgcgc cctgacgggc ttgtctgctc ccggcatccg cttacagaca agctgtgacc
     2341 gtctccggga gctgcatgtg tcagaggttt tcaccgtcat caccgaaacg cgcgaggcag
     2401 ctgcggtaaa gctcatcagc gtggtcgtga agcgattcac agatgtctgc ctgttcatcc
     2461 gcgtccagct cgttgagttt ctccagaagc gttaatgtct ggcttctgat aaagcgggcc
     2521 atgttaaggg cggttttttc ctgtttggtc actgatgcct ccgtgtaagg gggatttctg
     2581 ttcatggggg taatgatacc gatgaaacga gagaggatgc tcacgatacg ggttactgat
     2641 gatgaacatg cccggttact ggaacgttgt gagggtaaac aactggcggt atggatgcgg
     2701 cgggaccaga gaaaaatcac tcagggtcaa tgccagcgct tcgttaatac agatgtaggt
     2761 gttccacagg gtagccagca gcatcctgcg atgcagatcc ggaacataat ggtgcagggc
     2821 gctgacttcc gcgtttccag actttacgaa acacggaaac cgaagatcat tcatgttgtt
     2881 gctcaggtcg cagacgtttt gcagcagcag tcgcttcacg ttcgctcgcg tatcggtgat
     2941 tcattctgct aaccagtaag gcaaccccgc cagcctagcc gggtcctcaa cgacaggagc
     3001 acgatcatgc gcacccgtgg ggccgccatg ccggcgataa tggcctgctt ctcgccgaaa
     3061 cgtttggtgg cgggaccagt gacgaaggct tgagcgaggg cgtgcaagat tccgaatacc
     3121 gcaagcgaca ggccgatcat cgtcgcgctc cagcgaaagc ggtcctcgcc gaaaatgacc
     3181 cagagcgctg ccggcacctg tcctacgagt tgcatgataa agaagatagt cataagtgcg
     3241 gcgacgatag tcatgccccg cgcccaccgg aaggagctga ctgggttgaa ggctctcaag
     3301 ggcatcggtc gagatcccgg tgcctaatga gtgagctaac ttacattaat tgcgttgcgc
     3361 tcactgcccg ctttccagtc gggaaacctg tcgtgccagc tgcattaatg aatcggccaa
     3421 cgcgcgggga gaggcggttt gcgtattggg cgccagggtg gtttttcttt tcaccagtga
     3481 gacgggcaac agctgattgc ccttcaccgc ctggccctga gagagttgca gcaagcggtc
     3541 cacgctggtt tgccccagca ggcgaaaatc ctgtttgatg gtggttaacg gcgggatata
     3601 acatgagcta tcttcggtat cgtcgtatcc cactaccgag atgtccgcac caacgcgcag
     3661 cccggactcg gtaatggcgc gcattgcgcc cagcgccatc tgatcgttgg caaccagcat
     3721 cgcagtggga acgatgccct cattcagcat ttgcatggtt tgttgaaaac cggacatggc
     3781 actccagtcg ccttcccgtt ccgctatcgg ctgaatttga ttgcgagtga gatatttatg
     3841 ccagccagcc agacgcagac gcgccgagac agaacttaat gggcccgcta acagcgcgat
     3901 ttgctggtga cccaatgcga ccagatgctc cacgcccagt cgcgtaccat cttcatggga
     3961 gaaaataata ctgttgatgg gtgtctggtc agagacatca agaaataacg ccggaacatt
     4021 agtgcaggca gcttccacag caatggcatc ctggtcatcc agcggatagt taatgatcag
     4081 cccactgacg cgttgcgcga gaagattgtg caccgccgct ttacaggctt cgacgccgct
     4141 tcgttctacc atcgacacca ccacgctggc acccagttga tcggcgcgag atttaatcgc
     4201 cgcgacaatt tgcgacggcg cgtgcagggc cagactggag gtggcaacgc caatcagcaa
     4261 cgactgtttg cccgccagtt gttgtgccac gcggttggga atgtaattca gctccgccat
     4321 cgccgcttcc actttttccc gcgttttcgc agaaacgtgg ctggcctggt tcaccacgcg
     4381 ggaaacggtc tgataagaga caccggcata ctctgcgaca tcgtataacg ttactggttt
     4441 cacattcacc accctgaatt gactctcttc cgggcgctat catgccatac cgcgaaaggt
     4501 tttgcgccat tcgatggtgt ccgggatctc gacgctctcc cttatgcgac tcctgcatta
     4561 ggaagcagcc cagtagtagg ttgaggccgt tgagcaccgc cgccgcaagg aatggtgcat
     4621 gcaaggagat ggcgcccaac agtcccccgg ccacggggcc tgccaccata cccacgccga
     4681 aacaagcgct catgagcccg aagtggcgag cccgatcttc cccatcggtg atgtcggcga
     4741 tataggcgcc agcaaccgca cctgtggcgc cggtgatgcc ggccacgatg cgtccggcgt
     4801 agaggatcga gatcgatctc gatcccgcga aattaatacg actcactata ggggaattgt
     4861 gagcggataa caattcccct ctagaaataa ttttgtttaa ctttaagaag gagatataca
     4921 tatgtttact atagagcaga ttacttcggc ttggttactt gtgagagccg gctcaagagg
     4981 agccggggtc gatggtatga ctgtcgatct attcgcagca ggagttaacg agcaacttag
     5041 aatattactg aggcagttgc agcaggagag ttatagagcc tctcctgcta agggattctt
     5101 cgtcgcaaag aagtcgggtg gaaagaggct gatcggaatc ccaactgttc gagataggat
     5161 agttcagaga ttacttttgg aagaattgta cttcccccta gaggacacct tcctagactg
     5221 tagttatgct tacagaccgg gtagaaacat ccaacaggcc gtgcagcacc tgtacagcta
     5281 ttatcaatac cagcccaagt ggatcattaa ggctgatatc gctgagttct tcgataatct
     5341 atgctgggct cttttattta cagccctgga agatctccaa ctcgaaccta tacttctaca
     5401 gctgttagag caacagttaa agtccggcat agttattgca ggcaagccaa tctatccagg
     5461 gaaaggtgta ttgcagggcg gggtcttgag tggagcactg gctaacttgt atcttacgtc
     5521 attcgagcga aagtgcctat cctacggtat caaccttgtt agatatggtg acgacttcgc
     5581 tatagcttgc tcgtcctggc ttgaggctaa caggatattg gataagatca ctacatggtt
     5641 aggcgagctg tacctcaacc tacaacctga gaaaacccag atcttcgctc cggacgacga
     5701 atttactttt ctaggttata gattcgctgg cggtgaggtg tatgccccgc ccccacctgt
     5761 aatgacgagg gaaggtgagt gggtcaccaa tgagtccgga ctgccatact tcaggcctaa
     5821 atctcgtcct gttaagttcg tgagtagacc acccaaagcc tgttctattg cttcaccaat
     5881 taagttccca acggccccaa ttagccactt atggcaggag ttcatgacta ccctatatgt
     5941 tacagatcag ggtgcatacc tttctgttaa gaaccaacaa ttccaagttt tctaccaggg
     6001 cgagctgaaa atcaaggtgc ctgctactag agtcaataac attgtaatgt tcggatgctg
     6061 caacgtcagt catggcgctg tcagtatggc cttgaggaga agaataccta ttatgtactt
     6121 atcacagaag ggtagatact tcggccacac cgctgttcag ggcgacgcac gagtggagta
     6181 cctcatgcag caagttaaat gttgcgaaaa tacacaattc acccgacaac aggccgaggc
     6241 tatcgtggct gctaaattac acaactccag aatcttactg atgagactca ataggagaag
     6301 gccgactgag atagcaacac aggccattga cttgatagaa atccttattg actcactgcc
     6361 acaggccgag tccctcgacg ccctaagagg ttacgaggga aaggccgcta ccgtctactt
     6421 tcaggcactc ggctcactat tcactggctt cttcgctttc gacaaacgta ctaaaagacc
     6481 acccacagat cccataaatt ctctgatgtc tctggggtat acccttttgt ctcaacaagt
     6541 attcagtttc attcaatcag taggcttgca tacacatttc ggtaacctac acacaccaag
     6601 ggacaaccac cctgccctcg ttagcgacct gatggaagaa tttcgagccc agatcgtgga
     6661 ctctttcgta gcatatctgg ttaacaagaa gatccttacc cctgaggact tcacaccccc
     6721 agatgagaga ggtggagtgt atctccaggc ttcagctctg aagaaatacc tgaaacactg
     6781 ggaagaaaag ttgcagacag aaactactca cccacatact ggttacaaag ttgcctacag
     6841 aagatgtatt gagctacaag ttagggaata catcgcttgc ctggtcggag aggtcgaagt
     6901 ctatcgacct atggtctgga agttgtagcg tacgttaact ttaagaagga gaacttaaga
     6961 tgttctactt ggtatgctat gacattgtgt cagataatcg aagaaacaaa gtatcaaagc
     7021 tgttggaagc atacggactt agagtccaga agtctgtgtt tgaatgcgtc ctagacgaaa
     7081 agcaatatga gatgttgtcc aagtatttga tgcgtctggt taacaggcga gaggaccaag
     7141 tcaggtttta tccaatgtcc gcccacaaca gatgcaaggt cgctgtgctg ggcacccagc
     7201 ccgagttcgt tgttgacgat gctgcattca tcgtgtgact cgagatccgg ctgctaacaa
     7261 agcccgaaag gaagctgagt tggctgctgc caccgctgag caataactag cataacccct
     7321 tggggcctct aaacgggtct tgaggggttt tttgctgaaa ggaggaacta tatccggata
//
