LOCUS       pFS_0385_pET30-R        7487 bp    DNA     circular UNA 25-AUG-2018
DEFINITION  Concatenation of 2 sequences.
ACCESSION   urn.local...36-9fa11gk
VERSION     urn.local...36-9fa11gk
KEYWORDS    .
SOURCE      
  ORGANISM  
            .
FEATURES             Location/Qualifiers
     promoter        7..156
                     /created_by="User"
                     /label="Leader"
     repeat_region   157..192
                     /created_by="User"
                     /label="DR"
     misc_feature    193..197
                     /created_by="User"
                     /label="FaqI"
     promoter        379..407
                     /Imported_from="<a
                     href=""http://wishart.biology.ualberta.ca/PlasMapper"">Pla
                     sMapper</a>"
                     /Transferred_From="amp prom"
                     /Transferred_Similarity="89.66%"
                     /label="amp prom"
     marker          complement(410..1225)
                     /Imported_from="<a
                     href=""http://wishart.biology.ualberta.ca/PlasMapper"">Pla
                     sMapper</a>"
                     /Transferred_From="kan2 marker"
                     /Transferred_Similarity="99.75%"
                     /label="kan2 marker"
     misc_feature    complement(411..1225)
                     /note="KanR"
                     /label="KanR"
     misc_feature    1331..1950
                     /Imported_from="<a
                     href=""http://wishart.biology.ualberta.ca/PlasMapper"">Pla
                     sMapper</a>"
                     /Transferred_From="pBR322 origin"
                     /Transferred_Similarity="99.68%"
                     /label="pBR322 origin"
                     /note="Geneious type: origin of replication"
     gene            complement(2362..2553)
                     /Imported_from="<a
                     href=""http://wishart.biology.ualberta.ca/PlasMapper"">Pla
                     sMapper</a>"
                     /Transferred_From="ROP"
                     /Transferred_Similarity="100.00%"
                     /label="ROP"
     misc_feature    complement(3362..4453)
                     /Imported_from="<a
                     href=""http://wishart.biology.ualberta.ca/PlasMapper"">Pla
                     sMapper</a>"
                     /Transferred_From="lacI reg"
                     /Transferred_Similarity="99.91%"
                     /label="lacI reg"
                     /note="Geneious type: regulatory sequence"
     promoter        4835..4853
                     /Imported_from="<a
                     href=""http://wishart.biology.ualberta.ca/PlasMapper"">Pla
                     sMapper</a>"
                     /Transferred_From="T7 prom"
                     /Transferred_Similarity="100.00%"
                     /label="T7 prom"
     misc_feature    4853..4880
                     /Imported_from="<a
                     href=""http://wishart.biology.ualberta.ca/PlasMapper"">Pla
                     sMapper</a>"
                     /Transferred_From="lacO reg"
                     /Transferred_Similarity="100.00%"
                     /label="lacO reg"
                     /note="Geneious type: regulatory sequence"
     gene            4898..4914
                     /Imported_from="<a
                     href=""http://wishart.biology.ualberta.ca/PlasMapper"">Pla
                     sMapper</a>"
                     /Transferred_From="T7_transl_en_RBS"
                     /Transferred_Similarity="100.00%"
                     /label="T7_transl_en_RBS"
     misc_feature    4920..4925
                     /Recognition_pattern="CA^TATG"
                     /annotation_group="CATATG"
                     /label="NdeI"
                     /note="Geneious type: restriction site"
     CDS             4923..6047
                     /created_by="User"
                     /label="Cas1"
     gene            6048..6064
                     /Imported_from="<a
                     href=""http://wishart.biology.ualberta.ca/PlasMapper"">Pla
                     sMapper</a>"
                     /X-Transferred_From="T7_transl_en_RBS"
                     /X-Transferred_Similarity="100.00%"
                     /Transferred_From="Digestion of
                     pFSNW_013_pET30bvectorNovagen_Golden Gate_T7_Terminator -
                     Fragment 2, Ligated sequences, Ligated sequences 2"
                     /Transferred_Similarity="100.00%"
                     /label="T7_transl_en_RBS"
     misc_feature    6070..6075
                     /Recognition_pattern="CA^TATG"
                     /annotation_group="CATATG"
                     /label="NdeI"
                     /note="Geneious type: restriction site"
     CDS             6076..7038
                     /created_by="User"
                     /label="RT"
     gene            7045..7061
                     /Imported_from="<a
                     href=""http://wishart.biology.ualberta.ca/PlasMapper"">Pla
                     sMapper</a>"
                     /X-Transferred_From="T7_transl_en_RBS"
                     /X-Transferred_Similarity="100.00%"
                     /Transferred_From="Digestion of
                     pFSNW_013_pET30bvectorNovagen_Golden Gate_T7_Terminator -
                     Fragment 2, Ligated sequences, Ligated sequences 2"
                     /Transferred_Similarity="100.00%"
                     /label="T7_transl_en_RBS"
     misc_feature    7064..7069
                     /Recognition_pattern="C^TTAAG"
                     /annotation_group="CTTAAG"
                     /label="AflII"
                     /note="Geneious type: restriction site"
     CDS             7070..7345
                     /created_by="User"
                     /label="Cas2"
     misc_feature    <7347..>7350
                     /note="XhoI"
                     /label="XhoI"
     terminator      7358..7486
                     /created_by="User"
                     /label="Terminator extended"
     terminator      7419..7462
                     /Imported_from="<a
                     href=""http://wishart.biology.ualberta.ca/PlasMapper"">Pla
                     sMapper</a>"
                     /Transferred_From="T7 term"
                     /Transferred_Similarity="97.73%"
                     /label="T7 term"
     misc_feature    complement(7487..>5)
                     /label="CsiI"
ORIGIN      
        1 ccaggttatt attccgttga gatgcgtcga ttgcttgcta ggcaagggtt tgaggtcggt
       61 gttttgcttg attttcgggc tcatatataa tgtttttcag agatgcgtcg atttggctcc
      121 tgtaaccctt actgggtaag ggctgctgag cgaactcccc accgattggg ttaattcgga
      181 ttagttggaa acgtcccgcg gccgctattc ttttgattta taagggattt tgccgatttc
      241 ggcctattgg ttaaaaaatg agctgattta acaaaaattt aacgcgaatt ttaacaaaat
      301 attaacgctt acaatttcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg
      361 tttatttttc taaatacatt caaatatgta tccgctcatg aattaattct tagaaaaact
      421 catcgagcat caaatgaaac tgcaatttat tcatatcagg attatcaata ccatattttt
      481 gaaaaagccg tttctgtaat gaaggagaaa actcaccgag gcagttccat aggatggcaa
      541 gatcctggta tcggtctgcg attccgactc gtccaacatc aatacaacct attaatttcc
      601 cctcgtcaaa aataaggtta tcaagtgaga aatcaccatg agtgacgact gaatccggtg
      661 agaatggcaa aagtttatgc atttctttcc agacttgttc aacaggccag ccattacgct
      721 cgtcatcaaa atcactcgca tcaaccaaac cgttattcat tcgtgattgc gcctgagcga
      781 gacgaaatac gcgatcgctg ttaaaaggac aattacaaac aggaatcgaa tgcaaccggc
      841 gcaggaacac tgccagcgca tcaacaatat tttcacctga atcaggatat tcttctaata
      901 cctggaatgc tgttttcccg gggatcgcag tggtgagtaa ccatgcatca tcaggagtac
      961 ggataaaatg cttgatggtc ggaagaggca taaattccgt cagccagttt agtctgacca
     1021 tctcatctgt aacatcattg gcaacgctac ctttgccatg tttcagaaac aactctggcg
     1081 catcgggctt cccatacaat cgatagattg tcgcacctga ttgcccgaca ttatcgcgag
     1141 cccatttata cccatataaa tcagcatcca tgttggaatt taatcgcggc ctagagcaag
     1201 acgtttcccg ttgaatatgg ctcataacac cccttgtatt actgtttatg taagcagaca
     1261 gttttattgt tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac
     1321 cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc
     1381 ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca
     1441 actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgtccttcta
     1501 gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct
     1561 ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg
     1621 gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc
     1681 acacagccca gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta
     1741 tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg
     1801 gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt
     1861 cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg
     1921 cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggt cttttgctgg
     1981 ccttttgctc acatgttctt tcctgcgtta tcccctgatt ctgtggataa ccgtattacc
     2041 gcctttgagt gagctgatac cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg
     2101 agcgaggaag cggaagagcg cctgatgcgg tattttctcc ttacgcatct gtgcggtatt
     2161 tcacaccgca atggtgcact ctcagtacaa tctgctctga tgccgcatag ttaagccagt
     2221 atacactccg ctatcgctac gtgactgggt catggctgcg ccccgacacc cgccaacacc
     2281 cgctgacgcg ccctgacggg cttgtctgct cccggcatcc gcttacagac aagctgtgac
     2341 cgtctccggg agctgcatgt gtcagaggtt ttcaccgtca tcaccgaaac gcgcgaggca
     2401 gctgcggtaa agctcatcag cgtggtcgtg aagcgattca cagatgtctg cctgttcatc
     2461 cgcgtccagc tcgttgagtt tctccagaag cgttaatgtc tggcttctga taaagcgggc
     2521 catgttaagg gcggtttttt cctgtttggt cactgatgcc tccgtgtaag ggggatttct
     2581 gttcatgggg gtaatgatac cgatgaaacg agagaggatg ctcacgatac gggttactga
     2641 tgatgaacat gcccggttac tggaacgttg tgagggtaaa caactggcgg tatggatgcg
     2701 gcgggaccag agaaaaatca ctcagggtca atgccagcgc ttcgttaata cagatgtagg
     2761 tgttccacag ggtagccagc agcatcctgc gatgcagatc cggaacataa tggtgcaggg
     2821 cgctgacttc cgcgtttcca gactttacga aacacggaaa ccgaagatca ttcatgttgt
     2881 tgctcaggtc gcagacgttt tgcagcagca gtcgcttcac gttcgctcgc gtatcggtga
     2941 ttcattctgc taaccagtaa ggcaaccccg ccagcctagc cgggtcctca acgacaggag
     3001 cacgatcatg cgcacccgtg gggccgccat gccggcgata atggcctgct tctcgccgaa
     3061 acgtttggtg gcgggaccag tgacgaaggc ttgagcgagg gcgtgcaaga ttccgaatac
     3121 cgcaagcgac aggccgatca tcgtcgcgct ccagcgaaag cggtcctcgc cgaaaatgac
     3181 ccagagcgct gccggcacct gtcctacgag ttgcatgata aagaagatag tcataagtgc
     3241 ggcgacgata gtcatgcccc gcgcccaccg gaaggagctg actgggttga aggctctcaa
     3301 gggcatcggt cgagatcccg gtgcctaatg agtgagctaa cttacattaa ttgcgttgcg
     3361 ctcactgccc gctttccagt cgggaaacct gtcgtgccag ctgcattaat gaatcggcca
     3421 acgcgcgggg agaggcggtt tgcgtattgg gcgccagggt ggtttttctt ttcaccagtg
     3481 agacgggcaa cagctgattg cccttcaccg cctggccctg agagagttgc agcaagcggt
     3541 ccacgctggt ttgccccagc aggcgaaaat cctgtttgat ggtggttaac ggcgggatat
     3601 aacatgagct atcttcggta tcgtcgtatc ccactaccga gatgtccgca ccaacgcgca
     3661 gcccggactc ggtaatggcg cgcattgcgc ccagcgccat ctgatcgttg gcaaccagca
     3721 tcgcagtggg aacgatgccc tcattcagca tttgcatggt ttgttgaaaa ccggacatgg
     3781 cactccagtc gccttcccgt tccgctatcg gctgaatttg attgcgagtg agatatttat
     3841 gccagccagc cagacgcaga cgcgccgaga cagaacttaa tgggcccgct aacagcgcga
     3901 tttgctggtg acccaatgcg accagatgct ccacgcccag tcgcgtacca tcttcatggg
     3961 agaaaataat actgttgatg ggtgtctggt cagagacatc aagaaataac gccggaacat
     4021 tagtgcaggc agcttccaca gcaatggcat cctggtcatc cagcggatag ttaatgatca
     4081 gcccactgac gcgttgcgcg agaagattgt gcaccgccgc tttacaggct tcgacgccgc
     4141 ttcgttctac catcgacacc accacgctgg cacccagttg atcggcgcga gatttaatcg
     4201 ccgcgacaat ttgcgacggc gcgtgcaggg ccagactgga ggtggcaacg ccaatcagca
     4261 acgactgttt gcccgccagt tgttgtgcca cgcggttggg aatgtaattc agctccgcca
     4321 tcgccgcttc cactttttcc cgcgttttcg cagaaacgtg gctggcctgg ttcaccacgc
     4381 gggaaacggt ctgataagag acaccggcat actctgcgac atcgtataac gttactggtt
     4441 tcacattcac caccctgaat tgactctctt ccgggcgcta tcatgccata ccgcgaaagg
     4501 ttttgcgcca ttcgatggtg tccgggatct cgacgctctc ccttatgcga ctcctgcatt
     4561 aggaagcagc ccagtagtag gttgaggccg ttgagcaccg ccgccgcaag gaatggtgca
     4621 tgcaaggaga tggcgcccaa cagtcccccg gccacggggc ctgccaccat acccacgccg
     4681 aaacaagcgc tcatgagccc gaagtggcga gcccgatctt ccccatcggt gatgtcggcg
     4741 atataggcgc cagcaaccgc acctgtggcg ccggtgatgc cggccacgat gcgtccggcg
     4801 tagaggatcg agatcgatct cgatcccgcg aaattaatac gactcactat aggggaattg
     4861 tgagcggata acaattcccc tctagaaata attttgttta actttaagaa ggagatatac
     4921 atatgggata cgagaacatc agataccttc acaagccgta cttcgcagaa acgattactg
     4981 agaagccacc acagactgag gaagatgact caccattcca gaagaacatc tggaacgctg
     5041 agatggctgc aatctatctt atcgagcagg gtacaaacat ctacaaagac taccagcgtt
     5101 tcataataca cgttagtgag aagccaaaac ttgaggtccc tatcagggac gtacaacaga
     5161 tcatcgtctt tggaaatatc cagctaagca cgcctgttat ccaggcctgc ctgaaggagc
     5221 agatcccagt tgttttcctg tctcagaccg gaacatacca tggccatctg tggtcggaga
     5281 agtcaatcca ccttgataac cagttggttc aggctgagcg aaggaacgac gacttattca
     5341 gattctcagt tagccgagca gtggttctag ggaagcttct gaactctaag caactactga
     5401 tgagattcaa cagaaggaga aagattggta aggtcgaaga ggcaatctac ggtatcaacc
     5461 aggacataga cgcattgaat tacgtggaca acctggacac gctgagggga tacgagggta
     5521 tcgcagccgc ccgttacttc cccgccttcg gtaacttgat aacaaaccca aagttcagct
     5581 tcagtcaaag gttcaggcag ccaccaactg acgaaattaa ctctctactg tccttcggtt
     5641 acacattgtt attcaacaac gttttatcct tcatcatcac tgagggcttg tcgccgtaca
     5701 taggacactt ccattacggt gacaagcaga aaacttacct cgcattcgac cttatggaag
     5761 agttcagatc gcctatcgta gactctcttg tattgaagat aattaacaag agcctattca
     5821 agccccaaga ctttgacgtt gtcgcctcta ccggcggtgt ttatttgtct cagaccagcc
     5881 gtagggtgtt cctgaagcaa ttcgagaaca gaatgaacga ggaaatctcc cacccggacc
     5941 tgatctccca ggttacttac cgtcatgcta tccagttgca ggtcagacga tacaaaaggt
     6001 gcctgctgag tgacaacatc tacgagtctt tcctaagggc tgactaatta actttaagaa
     6061 ggagatatac atatgatgat atcatctcag ttcatagaca taaacaactt ccagagagca
     6121 tgggagaaag tcgcagacaa gagagggtgt gctggagtag acggagagac aatctcctca
     6181 ttcgccagta accagactgt gaacgtctac caactcatga atagtgtggc agacggatct
     6241 taccagccgt tcccatgtaa acaagtcatc atcccaaaga ggaatgggtc ccagagggaa
     6301 ctaaagatac cgaccatcag ggataggata gtgcagcagg ctctcctaaa cgtcatctca
     6361 cctcttatgg aagaaaagtt ctctccagtc tctttcgcat acaggccaaa cttgagctac
     6421 attaacgccg tggagaagat cgcagattgg agagatatgg ggtacgtttg ggtcctggac
     6481 gccgacattg tgaaattctt cgacaacatc gatcaccaca gacttttgca gcaagtgcga
     6541 ctacacatcg atcaccctgg catcctgtgc ctgataaagg catggatctc tgtcggcgtc
     6601 gagacacgag agggtttgat tcttccacag aaaggaatcc cacagggcgc tgtcatctca
     6661 cctatcttag ctaacatcta cctgcacgag ttcgacgaga taatctctgc atctgaccta
     6721 gagatagtaa gatacgccga cgacttctta gtactatcca ccagccagga gagaatagcc
     6781 atagccaagt cccaggttat agacttgctc gactcattgg gtctagagat taacacagac
     6841 aagacacaga tcacgtcttt cgagagagga ttcagattcc ttggacacgg gtttctgtcc
     6901 gacgcaatat ttccagtgga caccaacaag gctaaactga aaagtggaat cgagacaaac
     6961 agagaaaaga ctaggacccg aaagacgagc aagaagaagc tctaccataa tccttacaga
     7021 aataagaagg tcgtatgacg tacgttaact ttaagaagga gaacttaaga tgcttgtggt
     7081 ggtggtttat gacattccca atgacaagag gcgaacaaaa ctgtctaact tcttggaagg
     7141 gtacggacag cgtgtgcagt tctcagtttt cgagtgcttc ttaaacctgg acgaaatgag
     7201 acagctattc gagaaaagca agaagattgt aaagccgtct gaggataacg tgagattcta
     7261 ctggatatca gaggacgcag tgtctcgtgt gcttaccata ggctctgagc accccaatgc
     7321 cccacctaat tattacgtca tctaactcga gatccggctg ctaacaaagc ccgaaaggaa
     7381 gctgagttgg ctgctgccac cgctgagcaa taactagcat aaccccttgg ggcctctaaa
     7441 cgggtcttga ggggtttttt gctgaaagga ggaactatat ccggata
//
