LOCUS       pFS_0387_pET30-R        7437 bp    DNA     circular UNA 25-AUG-2018
DEFINITION  Concatenation of 2 sequences.
ACCESSION   urn.local...38-9fa11gk
VERSION     urn.local...38-9fa11gk
KEYWORDS    .
SOURCE      
  ORGANISM  
            .
FEATURES             Location/Qualifiers
     promoter        180..208
                     /Imported_from="<a
                     href=""http://wishart.biology.ualberta.ca/PlasMapper"">Pla
                     sMapper</a>"
                     /Transferred_From="amp prom"
                     /Transferred_Similarity="89.66%"
                     /label="amp prom"
     marker          complement(211..1026)
                     /Imported_from="<a
                     href=""http://wishart.biology.ualberta.ca/PlasMapper"">Pla
                     sMapper</a>"
                     /Transferred_From="kan2 marker"
                     /Transferred_Similarity="99.75%"
                     /label="kan2 marker"
     misc_feature    complement(212..1026)
                     /note="KanR"
                     /label="KanR"
     misc_feature    1132..1751
                     /Imported_from="<a
                     href=""http://wishart.biology.ualberta.ca/PlasMapper"">Pla
                     sMapper</a>"
                     /Transferred_From="pBR322 origin"
                     /Transferred_Similarity="99.68%"
                     /label="pBR322 origin"
                     /note="Geneious type: origin of replication"
     gene            complement(2163..2354)
                     /Imported_from="<a
                     href=""http://wishart.biology.ualberta.ca/PlasMapper"">Pla
                     sMapper</a>"
                     /Transferred_From="ROP"
                     /Transferred_Similarity="100.00%"
                     /label="ROP"
     misc_feature    complement(3163..4254)
                     /Imported_from="<a
                     href=""http://wishart.biology.ualberta.ca/PlasMapper"">Pla
                     sMapper</a>"
                     /Transferred_From="lacI reg"
                     /Transferred_Similarity="99.91%"
                     /label="lacI reg"
                     /note="Geneious type: regulatory sequence"
     promoter        4636..4654
                     /Imported_from="<a
                     href=""http://wishart.biology.ualberta.ca/PlasMapper"">Pla
                     sMapper</a>"
                     /Transferred_From="T7 prom"
                     /Transferred_Similarity="100.00%"
                     /label="T7 prom"
     misc_feature    4654..4681
                     /Imported_from="<a
                     href=""http://wishart.biology.ualberta.ca/PlasMapper"">Pla
                     sMapper</a>"
                     /Transferred_From="lacO reg"
                     /Transferred_Similarity="100.00%"
                     /label="lacO reg"
                     /note="Geneious type: regulatory sequence"
     gene            4699..4715
                     /Imported_from="<a
                     href=""http://wishart.biology.ualberta.ca/PlasMapper"">Pla
                     sMapper</a>"
                     /Transferred_From="T7_transl_en_RBS"
                     /Transferred_Similarity="100.00%"
                     /label="T7_transl_en_RBS"
     misc_feature    4721..4726
                     /Recognition_pattern="CA^TATG"
                     /annotation_group="CATATG"
                     /label="NdeI"
                     /note="Geneious type: restriction site"
     CDS             4724..5848
                     /created_by="User"
                     /label="Cas1"
     gene            5849..5865
                     /Imported_from="<a
                     href=""http://wishart.biology.ualberta.ca/PlasMapper"">Pla
                     sMapper</a>"
                     /X-Transferred_From="T7_transl_en_RBS"
                     /X-Transferred_Similarity="100.00%"
                     /Transferred_From="Digestion of
                     pFSNW_013_pET30bvectorNovagen_Golden Gate_T7_Terminator -
                     Fragment 2, Ligated sequences, Ligated sequences 2"
                     /Transferred_Similarity="100.00%"
                     /label="T7_transl_en_RBS"
     misc_feature    5871..5876
                     /Recognition_pattern="CA^TATG"
                     /annotation_group="CATATG"
                     /label="NdeI"
                     /note="Geneious type: restriction site"
     CDS             5877..6839
                     /created_by="User"
                     /label="RT"
     gene            6846..6862
                     /Imported_from="<a
                     href=""http://wishart.biology.ualberta.ca/PlasMapper"">Pla
                     sMapper</a>"
                     /X-Transferred_From="T7_transl_en_RBS"
                     /X-Transferred_Similarity="100.00%"
                     /Transferred_From="Digestion of
                     pFSNW_013_pET30bvectorNovagen_Golden Gate_T7_Terminator -
                     Fragment 2, Ligated sequences, Ligated sequences 2"
                     /Transferred_Similarity="100.00%"
                     /label="T7_transl_en_RBS"
     misc_feature    6865..6870
                     /Recognition_pattern="C^TTAAG"
                     /annotation_group="CTTAAG"
                     /label="AflII"
                     /note="Geneious type: restriction site"
     CDS             6871..7146
                     /created_by="User"
                     /label="Cas2"
     misc_feature    <7148..>7151
                     /note="XhoI"
                     /label="XhoI"
     terminator      7159..7287
                     /created_by="User"
                     /label="Terminator extended"
     terminator      7220..7263
                     /Imported_from="<a
                     href=""http://wishart.biology.ualberta.ca/PlasMapper"">Pla
                     sMapper</a>"
                     /Transferred_From="T7 term"
                     /Transferred_Similarity="97.73%"
                     /label="T7 term"
     misc_feature    complement(7288..>7293)
                     /label="CsiI"
     promoter        7295..7394
                     /created_by="User"
                     /label="Leader"
     repeat_region   7395..7430
                     /created_by="User"
                     /label="DR"
     misc_feature    7431..7435
                     /created_by="User"
                     /label="FaqI"
ORIGIN      
        1 ggccgctatt cttttgattt ataagggatt ttgccgattt cggcctattg gttaaaaaat
       61 gagctgattt aacaaaaatt taacgcgaat tttaacaaaa tattaacgct tacaatttca
      121 ggtggcactt ttcggggaaa tgtgcgcgga acccctattt gtttattttt ctaaatacat
      181 tcaaatatgt atccgctcat gaattaattc ttagaaaaac tcatcgagca tcaaatgaaa
      241 ctgcaattta ttcatatcag gattatcaat accatatttt tgaaaaagcc gtttctgtaa
      301 tgaaggagaa aactcaccga ggcagttcca taggatggca agatcctggt atcggtctgc
      361 gattccgact cgtccaacat caatacaacc tattaatttc ccctcgtcaa aaataaggtt
      421 atcaagtgag aaatcaccat gagtgacgac tgaatccggt gagaatggca aaagtttatg
      481 catttctttc cagacttgtt caacaggcca gccattacgc tcgtcatcaa aatcactcgc
      541 atcaaccaaa ccgttattca ttcgtgattg cgcctgagcg agacgaaata cgcgatcgct
      601 gttaaaagga caattacaaa caggaatcga atgcaaccgg cgcaggaaca ctgccagcgc
      661 atcaacaata ttttcacctg aatcaggata ttcttctaat acctggaatg ctgttttccc
      721 ggggatcgca gtggtgagta accatgcatc atcaggagta cggataaaat gcttgatggt
      781 cggaagaggc ataaattccg tcagccagtt tagtctgacc atctcatctg taacatcatt
      841 ggcaacgcta cctttgccat gtttcagaaa caactctggc gcatcgggct tcccatacaa
      901 tcgatagatt gtcgcacctg attgcccgac attatcgcga gcccatttat acccatataa
      961 atcagcatcc atgttggaat ttaatcgcgg cctagagcaa gacgtttccc gttgaatatg
     1021 gctcataaca ccccttgtat tactgtttat gtaagcagac agttttattg ttcatgacca
     1081 aaatccctta acgtgagttt tcgttccact gagcgtcaga ccccgtagaa aagatcaaag
     1141 gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac
     1201 cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa
     1261 ctggcttcag cagagcgcag ataccaaata ctgtccttct agtgtagccg tagttaggcc
     1321 accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag
     1381 tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac
     1441 cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc
     1501 gaacgaccta caccgaactg agatacctac agcgtgagct atgagaaagc gccacgcttc
     1561 ccgaagggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca
     1621 cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc
     1681 tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg
     1741 ccagcaacgc ggccttttta cggttcctgg tcttttgctg gccttttgct cacatgttct
     1801 ttcctgcgtt atcccctgat tctgtggata accgtattac cgcctttgag tgagctgata
     1861 ccgctcgccg cagccgaacg accgagcgca gcgagtcagt gagcgaggaa gcggaagagc
     1921 gcctgatgcg gtattttctc cttacgcatc tgtgcggtat ttcacaccgc aatggtgcac
     1981 tctcagtaca atctgctctg atgccgcata gttaagccag tatacactcc gctatcgcta
     2041 cgtgactggg tcatggctgc gccccgacac ccgccaacac ccgctgacgc gccctgacgg
     2101 gcttgtctgc tcccggcatc cgcttacaga caagctgtga ccgtctccgg gagctgcatg
     2161 tgtcagaggt tttcaccgtc atcaccgaaa cgcgcgaggc agctgcggta aagctcatca
     2221 gcgtggtcgt gaagcgattc acagatgtct gcctgttcat ccgcgtccag ctcgttgagt
     2281 ttctccagaa gcgttaatgt ctggcttctg ataaagcggg ccatgttaag ggcggttttt
     2341 tcctgtttgg tcactgatgc ctccgtgtaa gggggatttc tgttcatggg ggtaatgata
     2401 ccgatgaaac gagagaggat gctcacgata cgggttactg atgatgaaca tgcccggtta
     2461 ctggaacgtt gtgagggtaa acaactggcg gtatggatgc ggcgggacca gagaaaaatc
     2521 actcagggtc aatgccagcg cttcgttaat acagatgtag gtgttccaca gggtagccag
     2581 cagcatcctg cgatgcagat ccggaacata atggtgcagg gcgctgactt ccgcgtttcc
     2641 agactttacg aaacacggaa accgaagatc attcatgttg ttgctcaggt cgcagacgtt
     2701 ttgcagcagc agtcgcttca cgttcgctcg cgtatcggtg attcattctg ctaaccagta
     2761 aggcaacccc gccagcctag ccgggtcctc aacgacagga gcacgatcat gcgcacccgt
     2821 ggggccgcca tgccggcgat aatggcctgc ttctcgccga aacgtttggt ggcgggacca
     2881 gtgacgaagg cttgagcgag ggcgtgcaag attccgaata ccgcaagcga caggccgatc
     2941 atcgtcgcgc tccagcgaaa gcggtcctcg ccgaaaatga cccagagcgc tgccggcacc
     3001 tgtcctacga gttgcatgat aaagaagata gtcataagtg cggcgacgat agtcatgccc
     3061 cgcgcccacc ggaaggagct gactgggttg aaggctctca agggcatcgg tcgagatccc
     3121 ggtgcctaat gagtgagcta acttacatta attgcgttgc gctcactgcc cgctttccag
     3181 tcgggaaacc tgtcgtgcca gctgcattaa tgaatcggcc aacgcgcggg gagaggcggt
     3241 ttgcgtattg ggcgccaggg tggtttttct tttcaccagt gagacgggca acagctgatt
     3301 gcccttcacc gcctggccct gagagagttg cagcaagcgg tccacgctgg tttgccccag
     3361 caggcgaaaa tcctgtttga tggtggttaa cggcgggata taacatgagc tatcttcggt
     3421 atcgtcgtat cccactaccg agatgtccgc accaacgcgc agcccggact cggtaatggc
     3481 gcgcattgcg cccagcgcca tctgatcgtt ggcaaccagc atcgcagtgg gaacgatgcc
     3541 ctcattcagc atttgcatgg tttgttgaaa accggacatg gcactccagt cgccttcccg
     3601 ttccgctatc ggctgaattt gattgcgagt gagatattta tgccagccag ccagacgcag
     3661 acgcgccgag acagaactta atgggcccgc taacagcgcg atttgctggt gacccaatgc
     3721 gaccagatgc tccacgccca gtcgcgtacc atcttcatgg gagaaaataa tactgttgat
     3781 gggtgtctgg tcagagacat caagaaataa cgccggaaca ttagtgcagg cagcttccac
     3841 agcaatggca tcctggtcat ccagcggata gttaatgatc agcccactga cgcgttgcgc
     3901 gagaagattg tgcaccgccg ctttacaggc ttcgacgccg cttcgttcta ccatcgacac
     3961 caccacgctg gcacccagtt gatcggcgcg agatttaatc gccgcgacaa tttgcgacgg
     4021 cgcgtgcagg gccagactgg aggtggcaac gccaatcagc aacgactgtt tgcccgccag
     4081 ttgttgtgcc acgcggttgg gaatgtaatt cagctccgcc atcgccgctt ccactttttc
     4141 ccgcgttttc gcagaaacgt ggctggcctg gttcaccacg cgggaaacgg tctgataaga
     4201 gacaccggca tactctgcga catcgtataa cgttactggt ttcacattca ccaccctgaa
     4261 ttgactctct tccgggcgct atcatgccat accgcgaaag gttttgcgcc attcgatggt
     4321 gtccgggatc tcgacgctct cccttatgcg actcctgcat taggaagcag cccagtagta
     4381 ggttgaggcc gttgagcacc gccgccgcaa ggaatggtgc atgcaaggag atggcgccca
     4441 acagtccccc ggccacgggg cctgccacca tacccacgcc gaaacaagcg ctcatgagcc
     4501 cgaagtggcg agcccgatct tccccatcgg tgatgtcggc gatataggcg ccagcaaccg
     4561 cacctgtggc gccggtgatg ccggccacga tgcgtccggc gtagaggatc gagatcgatc
     4621 tcgatcccgc gaaattaata cgactcacta taggggaatt gtgagcggat aacaattccc
     4681 ctctagaaat aattttgttt aactttaaga aggagatata catatgggat acgagaacat
     4741 cagatacctt cacaagccgt acttcgcaga aacgattact gagaagccac cacagactga
     4801 ggaagatgac tcaccattcc agaagaacat ctggaacgct gagatggctg caatctatct
     4861 tatcgagcag ggtacaaaca tctacaaaga ctaccagcgt ttcataatac acgttagtga
     4921 gaagccaaaa cttgaggtcc ctatcaggga cgtacaacag atcatcgtct ttggaaatat
     4981 ccagctaagc acgcctgtta tccaggcctg cctgaaggag cagatcccag ttgttttcct
     5041 gtctcagacc ggaacatacc atggccatct gtggtcggag aagtcaatcc accttgataa
     5101 ccagttggtt caggctgagc gaaggaacga cgacttattc agattctcag ttagccgagc
     5161 agtggttcta gggaagcttc tgaactctaa gcaactactg atgagattca acagaaggag
     5221 aaagattggt aaggtcgaag aggcaatcta cggtatcaac caggacatag acgcattgaa
     5281 ttacgtggac aacctggaca cgctgagggg atacgagggt atcgcagccg cccgttactt
     5341 ccccgccttc ggtaacttga taacaaaccc aaagttcagc ttcagtcaaa ggttcaggca
     5401 gccaccaact gacgaaatta actctctact gtccttcggt tacacattgt tattcaacaa
     5461 cgttttatcc ttcatcatca ctgagggctt gtcgccgtac ataggacact tccattacgg
     5521 tgacaagcag aaaacttacc tcgcattcga ccttatggaa gagttcagat cgcctatcgt
     5581 agactctctt gtattgaaga taattaacaa gagcctattc aagccccaag actttgacgt
     5641 tgtcgcctct accggcggtg tttatttgtc tcagaccagc cgtagggtgt tcctgaagca
     5701 attcgagaac agaatgaacg aggaaatctc ccacccggac ctgatctccc aggttactta
     5761 ccgtcatgct atccagttgc aggtcagacg atacaaaagg tgcctgctga gtgacaacat
     5821 ctacgagtct ttcctaaggg ctgactaatt aactttaaga aggagatata catatgatga
     5881 tatcatctca gttcatagac ataaacaact tccagagagc atgggagaaa gtcgcagaca
     5941 agagagggtg tgctggagta gacggagaga caatctcctc attcgccagt aaccagactg
     6001 tgaacgtcta ccaactcatg aatagtgtgg cagacggatc ttaccagccg ttcccatgta
     6061 aacaagtcat catcccaaag aggaatgggt cccagaggga actaaagata ccgaccatca
     6121 gggataggat agtgcagcag gctctcctaa acgtcatctc acctcttatg gaagaaaagt
     6181 tctctccagt ctctttcgca tacaggccaa acttgagcta cattaacgcc gtggagaaga
     6241 tcgcagattg gagagatatg gggtacgttt gggtcctgga cgccgacatt gtgaaattct
     6301 tcgacaacat cgatcaccac agacttttgc agcaagtgcg actacacatc gatcaccctg
     6361 gcatcctgtg cctgataaag gcatggatct ctgtcggcgt cgagacacga gagggtttga
     6421 ttcttccaca gaaaggaatc ccacagggcg ctgtcatctc acctatctta gctaacatct
     6481 acctgcacga gttcgacgag ataatctctg catctgacct agagatagta agatacgccg
     6541 acgacttctt agtactatcc accagccagg agagaatagc catagccaag tcccaggtta
     6601 tagacttgct cgactcattg ggtctagaga ttaacacaga caagacacag atcacgtctt
     6661 tcgagagagg attcagattc cttggacacg ggtttctgtc cgacgcaata tttccagtgg
     6721 acaccaacaa ggctaaactg aaaagtggaa tcgagacaaa cagagaaaag actaggaccc
     6781 gaaagacgag caagaagaag ctctaccata atccttacag aaataagaag gtcgtatgac
     6841 gtacgttaac tttaagaagg agaacttaag atgcttgtgg tggtggttta tgacattccc
     6901 aatgacaaga ggcgaacaaa actgtctaac ttcttggaag ggtacggaca gcgtgtgcag
     6961 ttctcagttt tcgagtgctt cttaaacctg gacgaaatga gacagctatt cgagaaaagc
     7021 aagaagattg taaagccgtc tgaggataac gtgagattct actggatatc agaggacgca
     7081 gtgtctcgtg tgcttaccat aggctctgag caccccaatg ccccacctaa ttattacgtc
     7141 atctaactcg agatccggct gctaacaaag cccgaaagga agctgagttg gctgctgcca
     7201 ccgctgagca ataactagca taaccccttg gggcctctaa acgggtcttg aggggttttt
     7261 tgctgaaagg aggaactata tccggatacc aggtaaagca gaagtggaag cactttgtgg
     7321 cagtacgctt gtggaagtac ttcttggcag ttactgccat cttctcgttc aagttacaag
     7381 atgctcggtt gaaaccccac cgattgggtt aattcggatt agttggaaac gtcccgc
//
