LOCUS       pFS_0392_pET30-R        7270 bp    DNA     circular UNA 25-AUG-2018
DEFINITION  Concatenation of 3 sequences.
ACCESSION   urn.local...3d-9fa11gl
VERSION     urn.local...3d-9fa11gl
KEYWORDS    .
SOURCE      
  ORGANISM  
            .
FEATURES             Location/Qualifiers
     promoter        7..>145
                     /created_by="User"
                     /label="Leader"
     promoter        <141..156
                     /created_by="User"
                     /label="Leader"
     repeat_region   157..186
                     /created_by="User"
                     /label="DR"
     misc_feature    187..191
                     /created_by="User"
                     /label="FaqI"
     promoter        373..401
                     /Imported_from="<a
                     href=""http://wishart.biology.ualberta.ca/PlasMapper"">Pla
                     sMapper</a>"
                     /Transferred_From="amp prom"
                     /Transferred_Similarity="89.66%"
                     /label="amp prom"
     marker          complement(404..1219)
                     /Imported_from="<a
                     href=""http://wishart.biology.ualberta.ca/PlasMapper"">Pla
                     sMapper</a>"
                     /Transferred_From="kan2 marker"
                     /Transferred_Similarity="99.75%"
                     /label="kan2 marker"
     misc_feature    complement(405..1219)
                     /note="KanR"
                     /label="KanR"
     misc_feature    1325..1944
                     /Imported_from="<a
                     href=""http://wishart.biology.ualberta.ca/PlasMapper"">Pla
                     sMapper</a>"
                     /Transferred_From="pBR322 origin"
                     /Transferred_Similarity="99.68%"
                     /label="pBR322 origin"
                     /note="Geneious type: origin of replication"
     gene            complement(2356..2547)
                     /Imported_from="<a
                     href=""http://wishart.biology.ualberta.ca/PlasMapper"">Pla
                     sMapper</a>"
                     /Transferred_From="ROP"
                     /Transferred_Similarity="100.00%"
                     /label="ROP"
     misc_feature    complement(3356..4447)
                     /Imported_from="<a
                     href=""http://wishart.biology.ualberta.ca/PlasMapper"">Pla
                     sMapper</a>"
                     /Transferred_From="lacI reg"
                     /Transferred_Similarity="99.91%"
                     /label="lacI reg"
                     /note="Geneious type: regulatory sequence"
     promoter        4829..4847
                     /Imported_from="<a
                     href=""http://wishart.biology.ualberta.ca/PlasMapper"">Pla
                     sMapper</a>"
                     /Transferred_From="T7 prom"
                     /Transferred_Similarity="100.00%"
                     /label="T7 prom"
     misc_feature    4847..4874
                     /Imported_from="<a
                     href=""http://wishart.biology.ualberta.ca/PlasMapper"">Pla
                     sMapper</a>"
                     /Transferred_From="lacO reg"
                     /Transferred_Similarity="100.00%"
                     /label="lacO reg"
                     /note="Geneious type: regulatory sequence"
     gene            4892..4908
                     /Imported_from="<a
                     href=""http://wishart.biology.ualberta.ca/PlasMapper"">Pla
                     sMapper</a>"
                     /Transferred_From="T7_transl_en_RBS"
                     /Transferred_Similarity="100.00%"
                     /label="T7_transl_en_RBS"
     misc_feature    4914..4919
                     /Recognition_pattern="CA^TATG"
                     /annotation_group="CATATG"
                     /label="NdeI"
                     /note="Geneious type: restriction site"
     CDS             4917..6836
                     /created_by="User"
                     /label="Cas1"
     gene            6843..6859
                     /Imported_from="<a
                     href=""http://wishart.biology.ualberta.ca/PlasMapper"">Pla
                     sMapper</a>"
                     /X-Transferred_From="T7_transl_en_RBS"
                     /X-Transferred_Similarity="100.00%"
                     /Transferred_From="Digestion of
                     pFSNW_013_pET30bvectorNovagen_Golden Gate_T7_Terminator -
                     Fragment 2, Ligated sequences, Ligated sequences 2"
                     /Transferred_Similarity="100.00%"
                     /label="T7_transl_en_RBS"
     misc_feature    6862..6867
                     /Recognition_pattern="C^TTAAG"
                     /annotation_group="CTTAAG"
                     /label="AflII"
                     /note="Geneious type: restriction site"
     CDS             6868..7128
                     /created_by="User"
                     /label="Cas2"
     misc_feature    <7130..>7133
                     /note="XhoI"
                     /label="XhoI"
     terminator      7141..7269
                     /created_by="User"
                     /label="Terminator extended"
     terminator      7202..7245
                     /Imported_from="<a
                     href=""http://wishart.biology.ualberta.ca/PlasMapper"">Pla
                     sMapper</a>"
                     /Transferred_From="T7 term"
                     /Transferred_Similarity="97.73%"
                     /label="T7 term"
     misc_feature    complement(7270..>5)
                     /label="CsiI"
ORIGIN      
        1 ccaggtgaac actgaacgtt gctcatgtgt aaggtatttt cggatggttt tcatcacctt
       61 gggatctcgc ttagccttaa catcatatgt gataatgacg aacatctctt cctccttgta
      121 ttttgatttt agtattccaa cctggtgaat ataggtgttt ccattcctta taggtaaggt
      181 acttacgtcc cgcggccgct attcttttga tttataaggg attttgccga tttcggccta
      241 ttggttaaaa aatgagctga tttaacaaaa atttaacgcg aattttaaca aaatattaac
      301 gcttacaatt tcaggtggca cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt
      361 tttctaaata cattcaaata tgtatccgct catgaattaa ttcttagaaa aactcatcga
      421 gcatcaaatg aaactgcaat ttattcatat caggattatc aataccatat ttttgaaaaa
      481 gccgtttctg taatgaagga gaaaactcac cgaggcagtt ccataggatg gcaagatcct
      541 ggtatcggtc tgcgattccg actcgtccaa catcaataca acctattaat ttcccctcgt
      601 caaaaataag gttatcaagt gagaaatcac catgagtgac gactgaatcc ggtgagaatg
      661 gcaaaagttt atgcatttct ttccagactt gttcaacagg ccagccatta cgctcgtcat
      721 caaaatcact cgcatcaacc aaaccgttat tcattcgtga ttgcgcctga gcgagacgaa
      781 atacgcgatc gctgttaaaa ggacaattac aaacaggaat cgaatgcaac cggcgcagga
      841 acactgccag cgcatcaaca atattttcac ctgaatcagg atattcttct aatacctgga
      901 atgctgtttt cccggggatc gcagtggtga gtaaccatgc atcatcagga gtacggataa
      961 aatgcttgat ggtcggaaga ggcataaatt ccgtcagcca gtttagtctg accatctcat
     1021 ctgtaacatc attggcaacg ctacctttgc catgtttcag aaacaactct ggcgcatcgg
     1081 gcttcccata caatcgatag attgtcgcac ctgattgccc gacattatcg cgagcccatt
     1141 tatacccata taaatcagca tccatgttgg aatttaatcg cggcctagag caagacgttt
     1201 cccgttgaat atggctcata acaccccttg tattactgtt tatgtaagca gacagtttta
     1261 ttgttcatga ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc agaccccgta
     1321 gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa
     1381 acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt
     1441 tttccgaagg taactggctt cagcagagcg cagataccaa atactgtcct tctagtgtag
     1501 ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta
     1561 atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca
     1621 agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag
     1681 cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gctatgagaa
     1741 agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga
     1801 acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc
     1861 gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc
     1921 ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggtcttttg ctggcctttt
     1981 gctcacatgt tctttcctgc gttatcccct gattctgtgg ataaccgtat taccgccttt
     2041 gagtgagctg ataccgctcg ccgcagccga acgaccgagc gcagcgagtc agtgagcgag
     2101 gaagcggaag agcgcctgat gcggtatttt ctccttacgc atctgtgcgg tatttcacac
     2161 cgcaatggtg cactctcagt acaatctgct ctgatgccgc atagttaagc cagtatacac
     2221 tccgctatcg ctacgtgact gggtcatggc tgcgccccga cacccgccaa cacccgctga
     2281 cgcgccctga cgggcttgtc tgctcccggc atccgcttac agacaagctg tgaccgtctc
     2341 cgggagctgc atgtgtcaga ggttttcacc gtcatcaccg aaacgcgcga ggcagctgcg
     2401 gtaaagctca tcagcgtggt cgtgaagcga ttcacagatg tctgcctgtt catccgcgtc
     2461 cagctcgttg agtttctcca gaagcgttaa tgtctggctt ctgataaagc gggccatgtt
     2521 aagggcggtt ttttcctgtt tggtcactga tgcctccgtg taagggggat ttctgttcat
     2581 gggggtaatg ataccgatga aacgagagag gatgctcacg atacgggtta ctgatgatga
     2641 acatgcccgg ttactggaac gttgtgaggg taaacaactg gcggtatgga tgcggcggga
     2701 ccagagaaaa atcactcagg gtcaatgcca gcgcttcgtt aatacagatg taggtgttcc
     2761 acagggtagc cagcagcatc ctgcgatgca gatccggaac ataatggtgc agggcgctga
     2821 cttccgcgtt tccagacttt acgaaacacg gaaaccgaag atcattcatg ttgttgctca
     2881 ggtcgcagac gttttgcagc agcagtcgct tcacgttcgc tcgcgtatcg gtgattcatt
     2941 ctgctaacca gtaaggcaac cccgccagcc tagccgggtc ctcaacgaca ggagcacgat
     3001 catgcgcacc cgtggggccg ccatgccggc gataatggcc tgcttctcgc cgaaacgttt
     3061 ggtggcggga ccagtgacga aggcttgagc gagggcgtgc aagattccga ataccgcaag
     3121 cgacaggccg atcatcgtcg cgctccagcg aaagcggtcc tcgccgaaaa tgacccagag
     3181 cgctgccggc acctgtccta cgagttgcat gataaagaag atagtcataa gtgcggcgac
     3241 gatagtcatg ccccgcgccc accggaagga gctgactggg ttgaaggctc tcaagggcat
     3301 cggtcgagat cccggtgcct aatgagtgag ctaacttaca ttaattgcgt tgcgctcact
     3361 gcccgctttc cagtcgggaa acctgtcgtg ccagctgcat taatgaatcg gccaacgcgc
     3421 ggggagaggc ggtttgcgta ttgggcgcca gggtggtttt tcttttcacc agtgagacgg
     3481 gcaacagctg attgcccttc accgcctggc cctgagagag ttgcagcaag cggtccacgc
     3541 tggtttgccc cagcaggcga aaatcctgtt tgatggtggt taacggcggg atataacatg
     3601 agctatcttc ggtatcgtcg tatcccacta ccgagatgtc cgcaccaacg cgcagcccgg
     3661 actcggtaat ggcgcgcatt gcgcccagcg ccatctgatc gttggcaacc agcatcgcag
     3721 tgggaacgat gccctcattc agcatttgca tggtttgttg aaaaccggac atggcactcc
     3781 agtcgccttc ccgttccgct atcggctgaa tttgattgcg agtgagatat ttatgccagc
     3841 cagccagacg cagacgcgcc gagacagaac ttaatgggcc cgctaacagc gcgatttgct
     3901 ggtgacccaa tgcgaccaga tgctccacgc ccagtcgcgt accatcttca tgggagaaaa
     3961 taatactgtt gatgggtgtc tggtcagaga catcaagaaa taacgccgga acattagtgc
     4021 aggcagcttc cacagcaatg gcatcctggt catccagcgg atagttaatg atcagcccac
     4081 tgacgcgttg cgcgagaaga ttgtgcaccg ccgctttaca ggcttcgacg ccgcttcgtt
     4141 ctaccatcga caccaccacg ctggcaccca gttgatcggc gcgagattta atcgccgcga
     4201 caatttgcga cggcgcgtgc agggccagac tggaggtggc aacgccaatc agcaacgact
     4261 gtttgcccgc cagttgttgt gccacgcggt tgggaatgta attcagctcc gccatcgccg
     4321 cttccacttt ttcccgcgtt ttcgcagaaa cgtggctggc ctggttcacc acgcgggaaa
     4381 cggtctgata agagacaccg gcatactctg cgacatcgta taacgttact ggtttcacat
     4441 tcaccaccct gaattgactc tcttccgggc gctatcatgc cataccgcga aaggttttgc
     4501 gccattcgat ggtgtccggg atctcgacgc tctcccttat gcgactcctg cattaggaag
     4561 cagcccagta gtaggttgag gccgttgagc accgccgccg caaggaatgg tgcatgcaag
     4621 gagatggcgc ccaacagtcc cccggccacg gggcctgcca ccatacccac gccgaaacaa
     4681 gcgctcatga gcccgaagtg gcgagcccga tcttccccat cggtgatgtc ggcgatatag
     4741 gcgccagcaa ccgcacctgt ggcgccggtg atgccggcca cgatgcgtcc ggcgtagagg
     4801 atcgagatcg atctcgatcc cgcgaaatta atacgactca ctatagggga attgtgagcg
     4861 gataacaatt cccctctaga aataattttg tttaacttta agaaggagat atacatatga
     4921 agaaactgac tatggaagat atcttcatat ccgagcaaat cgaggaagcc ctcgatcacc
     4981 tgtctactaa gaaggacaca tgcggtattg acggtttgta cttgtccgag cttagggacg
     5041 actggaacat caacggtgag aggtacttgt ccctcttgcg taaggggaaa tataagccgg
     5101 gtatcgtgca gatttacgag attgttaact acaccgggaa gcgtagatcc atttcctctt
     5161 ttaattccat agataggcta gtcctgagat gccttgctac aagtctggag aagtactacg
     5221 actcgatctt ctctagctcc tcgttcgctt tccgaccagg gctcggggtg gacaaagcag
     5281 tcgccacgtt cgccaacaac ctgaacaccg gactgaccag ggtagcaatc atcgacatca
     5341 aacactactt cgattctatt ccaatcgatc gtttggagat gatactgaag cgaatcatag
     5401 acgacaacgt cttattgagt ctattccaca acttacttta ttgccgtatc tctgaggaga
     5461 acgtaatcaa gactaagtct aagggcatac tccagggatc accaatatct ccattccttg
     5521 gtaacttgta cttatcttta ctagatacac agctggagtc tatgcacgta tcattttgca
     5581 gatactgcga cgacatcgct atgttcttcg cttctttcga ggaagctaag gaaacttaca
     5641 ctaaggtcta cgatatcttg aagaacgacc tggagatgga cattaaccca cagaagtcag
     5701 ggatctacga aggcatcaag cagaactact tgggttatag tttcactaag aacaagaagg
     5761 agcaccagat cttagctatc aagaagaaga aggctccacc tcagatttac cagcactggt
     5821 ccaccaccgc aatccagagg gtcgatagga attatcatat cattaacaac ggtattctta
     5881 accgtaagga cttcaccctc ttgttcgaga acgacagagg caagaaatat ctccctgttg
     5941 aagccaccga gtccctaaac gtgtacacaa gtgtaatttt ctcctcagac ttcttcaagt
     6001 acgtaggcaa caagaaaatc tgcgtcaaca tctttgataa gtatggggag ttggccggaa
     6061 ctttcagtcc accggagtcc cttcacggtg gcctcaccat gttaaaacaa gctgccattt
     6121 acttggacga cgagaaaagg aagctgatcg ccaggaaatt ggaaattgca tccctgcata
     6181 acatgaggtc taatctgaag tactatgagc gtcaccactc ctctgaaaac ctgaaggacg
     6241 gtattacctc gttctctgaa tacattaccg ctatgaacga ggctacaaac atcgttatgc
     6301 tattaaccat tgaggcacga gcacgtcagt tgtattattc tctgtttcac gagatcattt
     6361 gcgacccagc tttcgagttc acgaagcgta cccgtaggcc accaaaagac cccttgaatg
     6421 ccttaatctc attcggcaac gttttcttgt ataacagaat cgccacggaa atccagaaaa
     6481 catcgttgga cataaggatc gggttcgttc acgctacaaa caggcgtaac caaagcctga
     6541 acctagatat agccgaccta ttcaagcccc tgatcgtgga tagagctatt ttcaccatca
     6601 ttaaccgaca catgatccac gcttcagagc acttcgagaa aacagaggac ggtggcattt
     6661 acctcaacaa agagggtaag cagatcttca ttaacgagtt ggagaacaag gtttatcaga
     6721 aacaaacaga ggagaacaaa cctcgtacct acgatactcg tatccgagag gagatccaca
     6781 agattttccg attagtttgt tacgacgaga agtataagcc attcaagtat aactaacgta
     6841 cgttaacttt aagaaggaga acttaagatg tttgtaatca taacctacga cgtaaaggcc
     6901 aagagggacc cgaaggtcat gaaaactata aggaagtact taacccacga gcagaggtct
     6961 gttttcgagg ggctcatcac gcccggtaga ttgaagcact tgaaggaaga acttaaaagg
     7021 attgtgaacg tgtcagagga ctgtatcaac atttactccc tggaaaccct gcgttacagt
     7081 aagaaggagt ccatcgggaa gcaagtttac cacggcaaca tcatttaact cgagatccgg
     7141 ctgctaacaa agcccgaaag gaagctgagt tggctgctgc caccgctgag caataactag
     7201 cataacccct tggggcctct aaacgggtct tgaggggttt tttgctgaaa ggaggaacta
     7261 tatccggata
//
