LOCUS       pFS_0414_pET30_M       12113 bp    DNA     circular UNA 25-AUG-2018
DEFINITION  Concatenation of 2 sequences.
ACCESSION   urn.local...3e-9fa11gn
VERSION     urn.local...3e-9fa11gn
KEYWORDS    .
SOURCE      
  ORGANISM  
            .
FEATURES             Location/Qualifiers
     promoter        180..208
                     /Imported_from="<a
                     href=""http://wishart.biology.ualberta.ca/PlasMapper"">Pla
                     sMapper</a>"
                     /Transferred_From="amp prom"
                     /Transferred_Similarity="89.66%"
                     /label="amp prom"
     marker          complement(211..1026)
                     /Imported_from="<a
                     href=""http://wishart.biology.ualberta.ca/PlasMapper"">Pla
                     sMapper</a>"
                     /Transferred_From="kan2 marker"
                     /Transferred_Similarity="99.75%"
                     /label="kan2 marker"
     misc_feature    complement(212..1026)
                     /note="KanR"
                     /label="KanR"
     misc_feature    1132..1751
                     /Imported_from="<a
                     href=""http://wishart.biology.ualberta.ca/PlasMapper"">Pla
                     sMapper</a>"
                     /Transferred_From="pBR322 origin"
                     /Transferred_Similarity="99.68%"
                     /label="pBR322 origin"
                     /note="Geneious type: origin of replication"
     gene            complement(2163..2354)
                     /Imported_from="<a
                     href=""http://wishart.biology.ualberta.ca/PlasMapper"">Pla
                     sMapper</a>"
                     /Transferred_From="ROP"
                     /Transferred_Similarity="100.00%"
                     /label="ROP"
     misc_feature    complement(3163..4254)
                     /Imported_from="<a
                     href=""http://wishart.biology.ualberta.ca/PlasMapper"">Pla
                     sMapper</a>"
                     /Transferred_From="lacI reg"
                     /Transferred_Similarity="99.91%"
                     /label="lacI reg"
                     /note="Geneious type: regulatory sequence"
     promoter        complement(4246..4323)
                     /gene="lacI"
                     /note="; lacI promoter"
                     /Transferred_From="lacI promoter"
                     /Transferred_Similarity="100.00%"
                     /label="lacI promoter"
     misc_feature    4425
                     /created_by="User"
                     /label
     promoter        4454..4488
                     /created_by="User"
                     /modified_by="User"
                     /label="BBa_J23100"
     source          <4489..>5418
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
                     /label="source synthetic DNA construct"
     RBS             4497..4508
                     /note="RBS (B0034)"
                     /label="RBS (B0034)"
     CDS             4515..5270
                     /codon_start=1
                     /product="LuxR-Barcode"
                     /note="luxR (C0062)"
                     /translation="MKNINADDTYRIINKIKACRSNNDINQCLSDMTKMVHCEYYLLA
                     IIYPHSMVKSDISILDNYPKKWRQYYDDANLIKYDPIVDYSNSNHSPINWNIFENNAV
                     NKKSPNVIKEAKTSGLITGFSFPIHTANNGFGMLSFAHSEKDNYIDSLFLHACMNIPL
                     IVPSLVDNYRKINIANNKSNNDLTKREKECLAWACEGKSSWDISKILGCSERTVTFHL
                     TNAQMKLNTTNRCQSISKAILTGAIDCPYFKN*"
                     /label="LuxR-Barcode CDS"
     CDS             4515..4517
                     /codon_start=1
                     /note="lasR (C0179)"
                     /translation="M"
                     /label="lasR (C0179) CDS"
     CDS             5265..5270
                     /codon_start=1
                     /note="lasR (C0179)"
                     /translation="*"
                     /label="lasR (C0179) CDS"
     terminator      5279..5407
                     /note="B0015"
                     /note="directional double term"
                     /label="B0015 terminator"
     source          <5425..>5483
                     /organism="recombinant plasmid"
                     /mol_type="other DNA"
                     /label="source recombinant plasmid"
     promoter        5425..5479
                     /note="lux rR (R0062)"
                     /label="lux rR (R0062) promoter"
     misc_feature    5480..5483
                     /label="GGC_overhang"
     CDS             5484..7136
                     /created_by="User"
                     /modified_by="User"
                     /label="Fluc"
     misc_feature    complement(7137..7139)
                     /label="1xStop_codon"
     source          <7142..>7260
                     /organism="recombinant plasmid"
                     /mol_type="other DNA"
                     /label="source recombinant plasmid"
     terminator      7144..7260
                     /note="T1"
                     /label="T1 terminator"
     terminator      7153..7239
                     /gene="Escherichia coli rrnB"
                     /note="T1"
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
                     /label="Escherichia coli rrnB terminator"
     protein_bind    7268..7305
                     /created_by="User"
                     /modified_by="User"
                     /label="operator: P(Cat)"
     misc_feature    <7268..>8190
                     /note="sequenced"
                     /note=150219
                     /note="3_pSUB36part c2_oLP218"
                     /label="3 pSUB36part c2 oLP218"
     -35_signal      7271..7276
                     /note
                     /label="-35 signal"
     -10_signal      7294..7299
                     /note
                     /label="-10 signal"
     RBS             7314..7325
                     /note="B0043"
                     /label="B0043 RBS"
     gene            7332..7952
                     /note="tetR"
                     /label="tetR gene"
     terminator      8003..8046
                     /note
                     /label="terminator"
     terminator      8087..8106
                     /note="T7 TE"
                     /label="T7 TE terminator"
     protein_bind    8129..8148
                     /note="tetR binding site"
                     /label="tetR binding site protein bind"
     promoter        8129..8186
                     /note="P_tetA"
                     /label="P tetA promoter"
     -35_signal      8148..8153
                     /note
                     /label="-35 signal"
     protein_bind    8154..8173
                     /note="tetR binding site"
                     /label="tetR binding site protein bind"
     -10_signal      8171..8176
                     /note
                     /label="-10 signal"
     misc_feature    8187..8190
                     /label="GGC_overhang"
     CDS             8191..9126
                     /gene="Rluc"
                     /label="Rluc CDS"
     misc_feature    8191..9126
                     /label="Rluc"
     misc_feature    complement(9127..9129)
                     /label="1xStop_codon"
     misc_feature    9130
                     /label="Golden_Gate"
     misc_feature    9135..9236
                     /created_by="User"
                     /label
     terminator      9148..9234
                     /gene="Escherichia coli rrnB"
                     /note="rrnB T1 terminator"
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
                     /modified_by="User"
                     /label="Escherichia coli rrnB terminator"
     promoter        9459..9477
                     /Imported_from="<a
                     href=""http://wishart.biology.ualberta.ca/PlasMapper"">Pla
                     sMapper</a>"
                     /Transferred_From="T7 prom"
                     /Transferred_Similarity="100.00%"
                     /label="T7 prom"
     misc_feature    9477..9504
                     /Imported_from="<a
                     href=""http://wishart.biology.ualberta.ca/PlasMapper"">Pla
                     sMapper</a>"
                     /Transferred_From="lacO reg"
                     /Transferred_Similarity="100.00%"
                     /label="lacO reg"
                     /note="Geneious type: regulatory sequence"
     protein_bind    complement(9478..9502)
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-beta-D-thiogalactopyranoside (IPTG).; lac
                     operator"
                     /Transferred_From="lac operator protein bind"
                     /Transferred_Similarity="100.00%"
                     /label="lac operator protein bind"
     gene            9522..9538
                     /Imported_from="<a
                     href=""http://wishart.biology.ualberta.ca/PlasMapper"">Pla
                     sMapper</a>"
                     /Transferred_From="T7_transl_en_RBS"
                     /Transferred_Similarity="100.00%"
                     /label="T7_transl_en_RBS"
     CDS             9547..11445
                     /created_by="User"
                     /label="Cas1"
     gene            11452..11468
                     /Imported_from="<a
                     href=""http://wishart.biology.ualberta.ca/PlasMapper"">Pla
                     sMapper</a>"
                     /X-Transferred_From="Digestion of
                     pFSNW_013_pET30bvectorNovagen_Golden Gate_T7_Terminator -
                     Fragment 2; Ligated sequences; Ligated sequences 2;
                     T7_transl_en_RBS"
                     /X-Transferred_Similarity="100.00%"
                     /Transferred_From="Digestion of
                     pFSNW_013_pET30bvectorNovagen_Golden Gate_T7_Terminator -
                     Fragment 2, Ligated sequences, Ligated sequences 2"
                     /Transferred_Similarity="100.00%"
                     /label="T7_transl_en_RBS"
     CDS             11477..11737
                     /created_by="User"
                     /label="Cas2"
     misc_feature    <11739..>11742
                     /note="XhoI"
                     /label="XhoI"
     terminator      11750..11878
                     /created_by="User"
                     /label="Terminator extended"
     terminator      11811..11854
                     /Imported_from="<a
                     href=""http://wishart.biology.ualberta.ca/PlasMapper"">Pla
                     sMapper</a>"
                     /Transferred_From="T7 term"
                     /Transferred_Similarity="97.73%"
                     /label="T7 term"
     misc_feature    complement(11879..>11884)
                     /label="CsiI"
     source          <11887..>12106
                     /organism="Fusicatenibacter saccharivorans"
                     /mol_type="genomic DNA"
                     /strain="2789STDY5608849"
                     /isolation_source="faeces"
                     /db_xref="taxon:1150298"
                     /country="United Kingdom"
                     /collection_date=2013
                     /note="contig: SCcontig000002"
                     /Transferred_From="NZ_CYYV01000002 - ARRAY 2"
                     /Transferred_Similarity="100.00%"
                     /label="contig: SCcontig000002 source Fusicatenibacter
                     saccharivorans"
     promoter        11887..12077
                     /created_by="User"
                     /label="Leader"
     repeat_region   12078..>12106
                     /inference="COORDINATES: alignment:crt:1.2; COORDINATES:
                     alignment:pilercr:v1.02"
                     /rpt_family="CRISPR"
                     /rpt_type="direct"
                     /rpt_unit_range="67767..67795"
                     /rpt_unit_seq="gtcgtactttacctaaaaggaattgaaac"
                     /Transferred_From="NZ_CYYV01000002 - ARRAY 2"
                     /Transferred_Similarity="100.00%"
                     /label="repeat region"
     misc_feature    12078..12106
                     /created_by="User"
                     /Transferred_From="NZ_CYYV01000002, NZ_CYYV01000002 -
                     ARRAY 2"
                     /Transferred_Similarity="100.00%"
                     /label="DR 2"
     source          <12107..>12111
                     /organism="Fusicatenibacter saccharivorans"
                     /mol_type="genomic DNA"
                     /strain="2789STDY5608849"
                     /isolation_source="faeces"
                     /db_xref="taxon:1150298"
                     /country="United Kingdom"
                     /collection_date=2013
                     /note="contig: SCcontig000002"
                     /Transferred_From="NZ_CYYV01000002 - ARRAY 1"
                     /Transferred_Similarity="100.00%"
                     /label="contig: SCcontig000002 source Fusicatenibacter
                     saccharivorans"
ORIGIN      
        1 ggccgctatt cttttgattt ataagggatt ttgccgattt cggcctattg gttaaaaaat
       61 gagctgattt aacaaaaatt taacgcgaat tttaacaaaa tattaacgct tacaatttca
      121 ggtggcactt ttcggggaaa tgtgcgcgga acccctattt gtttattttt ctaaatacat
      181 tcaaatatgt atccgctcat gaattaattc ttagaaaaac tcatcgagca tcaaatgaaa
      241 ctgcaattta ttcatatcag gattatcaat accatatttt tgaaaaagcc gtttctgtaa
      301 tgaaggagaa aactcaccga ggcagttcca taggatggca agatcctggt atcggtctgc
      361 gattccgact cgtccaacat caatacaacc tattaatttc ccctcgtcaa aaataaggtt
      421 atcaagtgag aaatcaccat gagtgacgac tgaatccggt gagaatggca aaagtttatg
      481 catttctttc cagacttgtt caacaggcca gccattacgc tcgtcatcaa aatcactcgc
      541 atcaaccaaa ccgttattca ttcgtgattg cgcctgagcg agacgaaata cgcgatcgct
      601 gttaaaagga caattacaaa caggaatcga atgcaaccgg cgcaggaaca ctgccagcgc
      661 atcaacaata ttttcacctg aatcaggata ttcttctaat acctggaatg ctgttttccc
      721 ggggatcgca gtggtgagta accatgcatc atcaggagta cggataaaat gcttgatggt
      781 cggaagaggc ataaattccg tcagccagtt tagtctgacc atctcatctg taacatcatt
      841 ggcaacgcta cctttgccat gtttcagaaa caactctggc gcatcgggct tcccatacaa
      901 tcgatagatt gtcgcacctg attgcccgac attatcgcga gcccatttat acccatataa
      961 atcagcatcc atgttggaat ttaatcgcgg cctagagcaa gacgtttccc gttgaatatg
     1021 gctcataaca ccccttgtat tactgtttat gtaagcagac agttttattg ttcatgacca
     1081 aaatccctta acgtgagttt tcgttccact gagcgtcaga ccccgtagaa aagatcaaag
     1141 gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac
     1201 cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa
     1261 ctggcttcag cagagcgcag ataccaaata ctgtccttct agtgtagccg tagttaggcc
     1321 accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag
     1381 tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac
     1441 cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc
     1501 gaacgaccta caccgaactg agatacctac agcgtgagct atgagaaagc gccacgcttc
     1561 ccgaagggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca
     1621 cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc
     1681 tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg
     1741 ccagcaacgc ggccttttta cggttcctgg tcttttgctg gccttttgct cacatgttct
     1801 ttcctgcgtt atcccctgat tctgtggata accgtattac cgcctttgag tgagctgata
     1861 ccgctcgccg cagccgaacg accgagcgca gcgagtcagt gagcgaggaa gcggaagagc
     1921 gcctgatgcg gtattttctc cttacgcatc tgtgcggtat ttcacaccgc aatggtgcac
     1981 tctcagtaca atctgctctg atgccgcata gttaagccag tatacactcc gctatcgcta
     2041 cgtgactggg tcatggctgc gccccgacac ccgccaacac ccgctgacgc gccctgacgg
     2101 gcttgtctgc tcccggcatc cgcttacaga caagctgtga ccgtctccgg gagctgcatg
     2161 tgtcagaggt tttcaccgtc atcaccgaaa cgcgcgaggc agctgcggta aagctcatca
     2221 gcgtggtcgt gaagcgattc acagatgtct gcctgttcat ccgcgtccag ctcgttgagt
     2281 ttctccagaa gcgttaatgt ctggcttctg ataaagcggg ccatgttaag ggcggttttt
     2341 tcctgtttgg tcactgatgc ctccgtgtaa gggggatttc tgttcatggg ggtaatgata
     2401 ccgatgaaac gagagaggat gctcacgata cgggttactg atgatgaaca tgcccggtta
     2461 ctggaacgtt gtgagggtaa acaactggcg gtatggatgc ggcgggacca gagaaaaatc
     2521 actcagggtc aatgccagcg cttcgttaat acagatgtag gtgttccaca gggtagccag
     2581 cagcatcctg cgatgcagat ccggaacata atggtgcagg gcgctgactt ccgcgtttcc
     2641 agactttacg aaacacggaa accgaagatc attcatgttg ttgctcaggt cgcagacgtt
     2701 ttgcagcagc agtcgcttca cgttcgctcg cgtatcggtg attcattctg ctaaccagta
     2761 aggcaacccc gccagcctag ccgggtcctc aacgacagga gcacgatcat gcgcacccgt
     2821 ggggccgcca tgccggcgat aatggcctgc ttctcgccga aacgtttggt ggcgggacca
     2881 gtgacgaagg cttgagcgag ggcgtgcaag attccgaata ccgcaagcga caggccgatc
     2941 atcgtcgcgc tccagcgaaa gcggtcctcg ccgaaaatga cccagagcgc tgccggcacc
     3001 tgtcctacga gttgcatgat aaagaagata gtcataagtg cggcgacgat agtcatgccc
     3061 cgcgcccacc ggaaggagct gactgggttg aaggctctca agggcatcgg tcgagatccc
     3121 ggtgcctaat gagtgagcta acttacatta attgcgttgc gctcactgcc cgctttccag
     3181 tcgggaaacc tgtcgtgcca gctgcattaa tgaatcggcc aacgcgcggg gagaggcggt
     3241 ttgcgtattg ggcgccaggg tggtttttct tttcaccagt gagacgggca acagctgatt
     3301 gcccttcacc gcctggccct gagagagttg cagcaagcgg tccacgctgg tttgccccag
     3361 caggcgaaaa tcctgtttga tggtggttaa cggcgggata taacatgagc tatcttcggt
     3421 atcgtcgtat cccactaccg agatgtccgc accaacgcgc agcccggact cggtaatggc
     3481 gcgcattgcg cccagcgcca tctgatcgtt ggcaaccagc atcgcagtgg gaacgatgcc
     3541 ctcattcagc atttgcatgg tttgttgaaa accggacatg gcactccagt cgccttcccg
     3601 ttccgctatc ggctgaattt gattgcgagt gagatattta tgccagccag ccagacgcag
     3661 acgcgccgag acagaactta atgggcccgc taacagcgcg atttgctggt gacccaatgc
     3721 gaccagatgc tccacgccca gtcgcgtacc atcttcatgg gagaaaataa tactgttgat
     3781 gggtgtctgg tcagagacat caagaaataa cgccggaaca ttagtgcagg cagcttccac
     3841 agcaatggca tcctggtcat ccagcggata gttaatgatc agcccactga cgcgttgcgc
     3901 gagaagattg tgcaccgccg ctttacaggc ttcgacgccg cttcgttcta ccatcgacac
     3961 caccacgctg gcacccagtt gatcggcgcg agatttaatc gccgcgacaa tttgcgacgg
     4021 cgcgtgcagg gccagactgg aggtggcaac gccaatcagc aacgactgtt tgcccgccag
     4081 ttgttgtgcc acgcggttgg gaatgtaatt cagctccgcc atcgccgctt ccactttttc
     4141 ccgcgttttc gcagaaacgt ggctggcctg gttcaccacg cgggaaacgg tctgataaga
     4201 gacaccggca tactctgcga catcgtataa cgttactggt ttcacattca ccaccctgaa
     4261 ttgactctct tccgggcgct atcatgccat accgcgaaag gttttgcgcc attcgatggt
     4321 gtccgggatc tcgacgctct cccttatgcg actcctgcat taggaagcag cccagtagta
     4381 ggttgaggcc gttgagcacc gccgccgcaa ggaatggtgc atgcaactag tatacagtga
     4441 ctcttggcgc gccttgacgg ctagctcagt cctaggtaca gtgctagcta ctagagaaag
     4501 aggagaaata ctagatgaaa aacataaatg ccgacgacac atacagaata attaataaaa
     4561 ttaaagcttg tagaagcaat aatgatatta atcaatgctt atctgatatg actaaaatgg
     4621 tacattgtga atattattta ctcgcgatca tttatcctca ttctatggtt aaatctgata
     4681 tttcaatcct agataattac cctaaaaaat ggaggcaata ttatgatgac gctaatttaa
     4741 taaaatatga tcctatagta gattattcta actccaatca ttcaccaatt aattggaata
     4801 tatttgaaaa caatgctgta aataaaaaat ctccaaatgt aattaaagaa gcgaaaacat
     4861 caggtcttat cactgggttt agtttcccta ttcatacggc taacaatggc ttcggaatgc
     4921 ttagttttgc acattcagaa aaagacaact atatagatag tttattttta catgcgtgta
     4981 tgaacatacc attaattgtt ccttctctag ttgataatta tcgaaaaata aatatagcaa
     5041 ataataaatc aaacaacgat ttaaccaaaa gagaaaaaga atgtttagcg tgggcatgcg
     5101 aaggaaaaag ctcttgggat atttcaaaaa tattaggttg cagtgagcgt actgtcactt
     5161 tccatttaac caatgcgcaa atgaaactca atacaacaaa ccgctgccaa agtatttcta
     5221 aagcaatttt aacaggagca attgattgcc catactttaa aaattaataa tactagagcc
     5281 aggcatcaaa taaaacgaaa ggctcagtcg aaagactggg cctttcgttt tatctgttgt
     5341 ttgtcggtga acgctctcta ctagagtcac actggctcac cttcgggtgg gcctttctgc
     5401 gtttataatt aaaggcatga attcacctgt aggatcgtac aggtttacgc aagaaaatgg
     5461 tttgttatag tcgaataaat actatggaag atgccaaaaa cataaagaaa ggcccggcgc
     5521 cattctatcc gctggaagat ggaaccgctg gagagcaact gcataaggct atgaagagat
     5581 acgccctggt tcctggaaca attgctttta cagatgcaca tatcgaggtg gacatcactt
     5641 acgctgagta cttcgaaatg tccgttcggt tggcagaagc tatgaaacga tatgggctga
     5701 atacaaatca cagaatcgtc gtatgcagtg aaaactctct tcaattcttt atgccggtgt
     5761 tgggcgcgtt atttatcgga gttgcagttg cgcccgcgaa cgacatttat aatgaacgtg
     5821 aattgctcaa cagtatgggc atttcgcagc ctaccgtggt gttcgtttcc aaaaaggggt
     5881 tgcaaaaaat tttgaacgtg caaaaaaagc tcccaatcat ccaaaaaatt attatcatgg
     5941 attctaaaac ggattaccag ggatttcagt cgatgtacac gttcgtcaca tctcatctac
     6001 ctcccggttt taatgaatac gattttgtgc cagagtcctt cgatagggac aagacaattg
     6061 cactgatcat gaactcctct ggatctactg gtctgcctaa aggtgtcgct ctgcctcata
     6121 gaactgcctg cgtgagattc tcgcatgcca gagatcctat ttttggcaat caaatcattc
     6181 cggatactgc gattttaagt gttgttccat tccatcacgg ttttggaatg tttactacac
     6241 tcggatattt gatatgtgga tttcgagtcg tcttaatgta tagatttgaa gaagagctgt
     6301 ttctgaggag ccttcaggat tacaagattc aaagtgcgct gctggtgcca accctattct
     6361 ccttcttcgc caaaagcact ctgattgaca aatacgattt atctaattta cacgaaattg
     6421 cttctggtgg cgctcccctc tctaaggaag tcggggaagc ggttgccaag aggttccatc
     6481 tgccaggtat caggcaagga tatgggctca ctgagactac atcagctatt ctgattacac
     6541 ccgaggggga tgataaaccg ggcgcggtcg gtaaagttgt tccatttttt gaagcgaagg
     6601 ttgtggatct ggataccggg aaaacgctgg gcgttaatca aagaggcgaa ctgtgtgtga
     6661 gaggtcctat gattatgtcc ggttatgtaa acaatccgga agcgaccaac gccttgattg
     6721 acaaggatgg atggctacat tctggagaca tagcttactg ggacgaagac gaacacttct
     6781 tcatcgttga ccgcctgaag tctctgatta agtacaaagg ctatcaggtg gctcccgctg
     6841 aattggaatc catcttgctc caacacccca acatcttcga cgcaggtgtc gcaggtcttc
     6901 ccgacgatga cgccggtgaa cttcccgccg ccgttgttgt tttggagcac ggaaagacga
     6961 tgacggaaaa agagatcgtg gattacgtcg ccagtcaagt aacaaccgcg aaaaagttgc
     7021 gcggaggagt tgtgtttgtg gacgaagtac cgaaaggtct taccggaaaa ctcgacgcaa
     7081 gaaaaatcag agagatcctc ataaaggcca agaagggcgg aaagatcgcc gtgtaatgac
     7141 gattaaaggc atcaaataaa acgaaaggct cagtcgaaag actgggcctt tcgttttatc
     7201 tgttgtttgt cggtgaacgc tctcctgagt aggacaaatc cgccgcccta gacagctggg
     7261 gtcgactggc acgtaagagg ttccaacttt caccataatg aaacatacta gagaaagagg
     7321 agaaatacta gatgtccaga ttagataaaa gtaaagtgat taacagcgca ttagagctgc
     7381 ttaatgaggt cggaatcgaa ggtttaacaa cccgtaaact cgcccagaag ctaggtgtag
     7441 agcagcctac attgtattgg catgtaaaaa ataagcgggc tttgctcgac gccttagcca
     7501 ttgagatgtt agataggcac catactcact tttgcccttt agaaggggaa agctggcaag
     7561 attttttacg taataacgct aaaagtttta gatgtgcttt actaagtcat cgcgatggag
     7621 caaaagtaca tttaggtaca cggcctacag aaaaacagta tgaaactctc gaaaatcaat
     7681 tagccttttt atgccaacaa ggtttttcac tagagaatgc attatatgca ctcagcgctg
     7741 tggggcattt tactttaggt tgcgtattgg aagatcaaga gcatcaagtc gctaaagaag
     7801 aaagggaaac acctactact gatagtatgc cgccattatt acgacaagct atcgaattat
     7861 ttgatcacca aggtgcagag ccagccttct tattcggcct tgaattgatc atatgcggat
     7921 tagaaaaaca acttaaatgt gaaagtgggt cctaataaca ctgatagtgc tagtgtagat
     7981 cactactaga gccaggcatc aaataaaacg aaaggctcag tcgaaagact gggcctttcg
     8041 ttttatctgt tgtttgtcgg tgaacgctct ctactagagt cacactggct caccttcggg
     8101 tgggcctttc tgcgtttata tactagagtc cctatcagtg atagagattg acatccctat
     8161 cagtgataga gatactgagc actactagag atggcttcca aggtgtacga ccccgagcaa
     8221 cgcaaacgca tgatcactgg gcctcagtgg tgggctcgct gcaagcaaat gaacgtgctg
     8281 gactccttca tcaactacta tgattccgag aagcacgccg agaacgccgt gatttttctg
     8341 catggtaacg ctgcctccag ctacctgtgg aggcacgtcg tgcctcacat cgagcccgtg
     8401 gctagatgca tcatccctga tctgatcgga atgggtaagt ccggcaagag cgggaatggc
     8461 tcatatcgcc tcctggatca ctacaagtac ctcaccgctt ggttcgagct gctgaacctt
     8521 ccaaagaaaa tcatctttgt gggccacgac tggggggctt gtctggcctt tcactactcc
     8581 tacgagcacc aagacaagat caaggccatc gtccatgctg agagtgtcgt ggacgtgatc
     8641 gagtcctggg acgagtggcc tgacatcgag gaggatatcg ccctgatcaa gagcgaagag
     8701 ggcgagaaaa tggtgcttga gaataacttc ttcgtcgaga ccatgctccc aagcaagatc
     8761 atgcggaaac tggagcctga ggagttcgct gcctacctgg agccattcaa ggagaagggc
     8821 gaggttagac ggcctaccct ctcctggcct cgcgagatcc ctctcgttaa gggaggcaag
     8881 cccgacgtcg tccagattgt ccgcaactac aacgcctacc ttcgggccag cgacgatctg
     8941 cctaagatgt tcatcgagtc cgaccctggg ttcttttcca acgctattgt cgagggagct
     9001 aagaagttcc ctaacaccga gttcgtgaag gtgaagggcc tccacttcag ccaggaggac
     9061 gctccagatg aaatgggtaa gtacatcaag agcttcgtgg agcgcgtgct gaagaacgag
     9121 cagtaatgag atcggatctc caggcatcaa ataaaacgaa aggctcagtc gaaagactgg
     9181 gcctttcgtt ttatctgttg tttgtcggtg aacgctctcc tgagtaggac aaatccggat
     9241 cccatgcaag gagatggcgc ccaacagtcc cccggccacg gggcctgcca ccatacccac
     9301 gccgaaacaa gcgctcatga gcccgaagtg gcgagcccga tcttccccat cggtgatgtc
     9361 ggcgatatag gcgccagcaa ccgcacctgt ggcgccggtg atgccggcca cgatgcgtcc
     9421 ggcgtagagg atcgagatcg atctcgatcc cgcgaaatta atacgactca ctatagggga
     9481 attgtgagcg gataacaatt cccctctaga aataattttg tttaacttta agaaggagat
     9541 atacatatgt tcactataga cgagatgcta tcaaagaaca accaaagatt ggctttcgag
     9601 cacttcgcta ctaagaacga cggatgtggc cctgatggta tgcacgtatc tgagctggag
     9661 aaatattggc gtatgaatca cgatcagatc atctccgact tgaagaacca agagtaccaa
     9721 cccggcatta ttttgattag agaacacatg aacaaaacag gtaagagaag gaacattgca
     9781 tctctcaacg tgatcgacag gttcataact cgactgctgt cgcaaaagtt gaatagatac
     9841 ttagccccca ttttctgcga gaactcatac gcttaccaag actcaaaagg ggtgatgccc
     9901 gccgtactaa aagccaagga gtacgtcgag ttgggtatga gacacgttat cgagatagac
     9961 ctcaagaatt acttcgacac gatcccgtta gagaacttaa tcccagagat cgagcgttac
    10021 ataacagatg aggcagtgct gcacttgatt aaacagtact tattctgtga catctcgttc
    10081 gagggtaaga tatcacgtaa aactcaaggg atcgttcagg gtaacgcaat ttccccaata
    10141 ctatccaacc tctacctgaa cgacttcgac aaggagctgg acgagtcgaa gctgtgctgg
    10201 atcagatacg ccgacaacat atacatctat atggacagct acgagaaggc cctactggtt
    10261 tattccgagc ttaccgagag acttgagaga aggaaattaa ccgttaacaa ggagaaatca
    10321 ggggtgtttg atgttagtac ccgttctata ctaggttacg acatcctgat cagaaacaag
    10381 aaggttgacg tgcgtaaaca catctacaag tctgttaacc agtactccaa ctggcacgac
    10441 tctcgattgg agttcatcaa cggtcgttac cacatcactt ccgacggtat cctcaataga
    10501 caagacttcg gtctactgtt cgagaatgag cagaagaagc actacatacc tgttgaggtt
    10561 tctgaccagt tgaatattta cggaaacgtt acgctcgcca gcaacgttct tcaatcattc
    10621 tccaaccgag agatcaaagt gtccttcttc gacaagtacg ggagacttat tggatctttc
    10681 ctgcccgaga aaactaagaa aagcgccgag ataatactgg ttcagtctaa gaactacctt
    10741 aacgaggacg ttaggatgga cacggcccga cgtatggaga tcgccggttt gcataacatc
    10801 agagccaacc tcagatacta cgacaagaag cacaaaggtg actttaagga aaaggtagac
    10861 gccatttctg gctatattga cgccctcaat agagcaccat ccgtgaacga catgatgctc
    10921 ttagaggcta aagctcgtca gctgtactac acatgcttca accagatctt agagacatcc
    10981 gactttcagt tcgagaagcg taccaagcga ccaccaaaag acgccatcaa cgcctgtatt
    11041 tcattcggca acacattact gtacaacttg tttgtgaata tcatttggaa gaagggactt
    11101 gatccaaggt tcggggttgt tcacgccagc aacaagcgta atcagtccct taacctggat
    11161 tttgctgaca tcttcaagcc tatagtcata gataggatca tcttcacaat gatcaacaag
    11221 aagatgctga ccttgttgac ggacttcgag acttctaacc agggagttta cctttcccga
    11281 gaggggaaga acatcttctt gcagatgtac gaagaaaagc tcaaatccag aatcactatc
    11341 aagggaaagg agatgagtta ctatcagttg ctagaatcag aggtgcagaa ctacaagaac
    11401 ttcatcctga ccggcgagac atacaagccg tacaagtact actagcgtac gttaacttta
    11461 agaaggagaa cttaagatgt atgtcattct agtttacgac attcaccaga agcgagtagg
    11521 taaggctcta aagatttgta ggaagtacct gatccacatc cagaagtcag ttttcgaagg
    11581 caacataacg gagagtaagc taaaggcctt aaaagaggag ctggggcacc tgatcgacac
    11641 tcagatggac tcggtaatta tctaccacct ggactccgtg aagtacacta agaaggagca
    11701 gatcggcatc gtccaatcga catccaatgt catttgactc gagatccggc tgctaacaaa
    11761 gcccgaaagg aagctgagtt ggctgctgcc accgctgagc aataactagc ataacccctt
    11821 ggggcctcta aacgggtctt gaggggtttt ttgctgaaag gaggaactat atccggatac
    11881 caggtgcggg gaagcttatg ttccatagca aaaagtcggt cagtctcgtg gctgaaatca
    11941 tgagttccac aaaatggctg aaattcaagg aaaatcagga atctcagaaa aacgatcgac
    12001 cgactttttc gataaaatgg ttgcaaaaat gagaaaaatc tgatttaata gaatctgaaa
    12061 acagcggaaa tgctgttgtc gtactttacc taaaaggaat tgaaacgtcc cgc
//
