LOCUS       SG_C_Int0               3398 bp ds-DNA     linear       15-AUG-2018
ACCESSION   New Construct
KEYWORDS    
SOURCE      Synthetic
  ORGANISM  Synthetic
COMMENT     
COMMENT     
COMMENT     ApEinfo:methylated:1
FEATURES             Location/Qualifiers
     source          1..1071
                     /organism="Synthetic"
                     /locus_tag="source:Synthetic"
                     /label="source:Synthetic"
                     /ApEinfo_label="source:Synthetic"
                     /ApEinfo_fwdcolor="pink"
                     /ApEinfo_revcolor="pink"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     rep_origin      complement(join(1302..1637,1658..2004))
                     /locus_tag="ColE1 origin"
                     /label="ColE1 origin"
                     /ApEinfo_label="ColE1 origin"
                     /ApEinfo_fwdcolor="gray50"
                     /ApEinfo_revcolor="gray50"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    1..40
                     /product="Spacer18"
                     /locus_tag="Spacer18"
                     /label="Spacer18"
                     /ApEinfo_label="Spacer18"
                     /ApEinfo_fwdcolor="pink"
                     /ApEinfo_revcolor="pink"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    3248..3266
                     /locus_tag="VF3"
                     /label="VF3"
                     /ApEinfo_label="VF3"
                     /ApEinfo_fwdcolor="#ff00ff"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    41..80
                     /product="Spacer21"
                     /locus_tag="Spacer21"
                     /label="Spacer21"
                     /ApEinfo_label="Spacer21"
                     /ApEinfo_fwdcolor="pink"
                     /ApEinfo_revcolor="pink"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    1149..1168
                     /locus_tag="VR primer sequence recognition "
                     /label="VR primer sequence recognition "
                     /ApEinfo_label="VR primer sequence recognition "
                     /ApEinfo_fwdcolor="cyan"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    81..116
                     /product="P5"
                     /locus_tag="P5"
                     /label="P5"
                     /ApEinfo_label="P5"
                     /ApEinfo_fwdcolor="pink"
                     /ApEinfo_revcolor="pink"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    1201..1220
                     /locus_tag="VR primer sequence recognition (1)"
                     /label="VR primer sequence recognition (1)"
                     /ApEinfo_label="VR primer sequence recognition "
                     /ApEinfo_fwdcolor="cyan"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    117..128
                     /product="rbs - B0034"
                     /locus_tag="rbs - B0034"
                     /label="rbs - B0034"
                     /ApEinfo_label="rbs - B0034"
                     /ApEinfo_fwdcolor="pink"
                     /ApEinfo_revcolor="pink"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    1638..1657
                     /locus_tag="VF2 primer"
                     /label="VF2 primer"
                     /ApEinfo_label="VF2 primer"
                     /ApEinfo_fwdcolor="#ff8000"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    129..134
                     /product="Spacer RBS"
                     /locus_tag="Spacer RBS"
                     /label="Spacer RBS"
                     /ApEinfo_label="Spacer RBS"
                     /ApEinfo_fwdcolor="pink"
                     /ApEinfo_revcolor="pink"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    1101..1117
                     /locus_tag="VR3"
                     /label="VR3"
                     /ApEinfo_label="VR3"
                     /ApEinfo_fwdcolor="#800080"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    135..174
                     /product="Spacer22"
                     /locus_tag="Spacer22"
                     /label="Spacer22"
                     /ApEinfo_label="Spacer22"
                     /ApEinfo_fwdcolor="pink"
                     /ApEinfo_revcolor="pink"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    3248..3266
                     /locus_tag="VF3(1)"
                     /label="VF3(1)"
                     /ApEinfo_label="VF3"
                     /ApEinfo_fwdcolor="#ff00ff"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    175..303
                     /product="B0015"
                     /locus_tag="B0015"
                     /label="B0015"
                     /ApEinfo_label="B0015"
                     /ApEinfo_fwdcolor="pink"
                     /ApEinfo_revcolor="pink"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    2129..3071
                     /locus_tag="Amp resistance"
                     /label="Amp resistance"
                     /ApEinfo_label="Amp resistance"
                     /ApEinfo_fwdcolor="#ccff66"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    304..343
                     /product="Spacer23"
                     /locus_tag="Spacer23"
                     /label="Spacer23"
                     /ApEinfo_label="Spacer23"
                     /ApEinfo_fwdcolor="pink"
                     /ApEinfo_revcolor="pink"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    2129..2156
                     /locus_tag="New Feature"
                     /label="New Feature"
                     /ApEinfo_label="New Feature"
                     /ApEinfo_fwdcolor="cyan"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    344..389
                     /product="P2"
                     /locus_tag="P2"
                     /label="P2"
                     /ApEinfo_label="P2"
                     /ApEinfo_fwdcolor="pink"
                     /ApEinfo_revcolor="pink"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    2129..2162
                     /locus_tag="New Feature(1)"
                     /label="New Feature(1)"
                     /ApEinfo_label="New Feature"
                     /ApEinfo_fwdcolor="cyan"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    390..402
                     /product="B0032"
                     /locus_tag="B0032"
                     /label="B0032"
                     /ApEinfo_label="B0032"
                     /ApEinfo_fwdcolor="pink"
                     /ApEinfo_revcolor="pink"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    3072..3100
                     /locus_tag="New Feature(2)"
                     /label="New Feature(2)"
                     /ApEinfo_label="New Feature"
                     /ApEinfo_fwdcolor="cyan"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    403..408
                     /product="Spacer RBS"
                     /locus_tag="Spacer RBS(1)"
                     /label="Spacer RBS(1)"
                     /ApEinfo_label="Spacer RBS"
                     /ApEinfo_fwdcolor="pink"
                     /ApEinfo_revcolor="pink"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    3397..3398
                     /locus_tag="prefix Bba"
                     /label="prefix Bba"
                     /ApEinfo_label="prefix Bba"
                     /ApEinfo_fwdcolor="#000000"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    409..448
                     /product="Spacer24"
                     /locus_tag="Spacer24"
                     /label="Spacer24"
                     /ApEinfo_label="Spacer24"
                     /ApEinfo_fwdcolor="pink"
                     /ApEinfo_revcolor="pink"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    3279..3298
                     /locus_tag="VF2 primer(1)"
                     /label="VF2 primer(1)"
                     /ApEinfo_label="VF2 primer"
                     /ApEinfo_fwdcolor="#ff8000"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    449..505
                     /product="L3S2P11"
                     /locus_tag="L3S2P11"
                     /label="L3S2P11"
                     /ApEinfo_label="L3S2P11"
                     /ApEinfo_fwdcolor="pink"
                     /ApEinfo_revcolor="pink"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    506..545
                     /product="Spacer25"
                     /locus_tag="Spacer25"
                     /label="Spacer25"
                     /ApEinfo_label="Spacer25"
                     /ApEinfo_fwdcolor="pink"
                     /ApEinfo_revcolor="pink"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    546..580
                     /product="J23100"
                     /locus_tag="J23100"
                     /label="J23100"
                     /ApEinfo_label="J23100"
                     /ApEinfo_fwdcolor="pink"
                     /ApEinfo_revcolor="pink"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    581..597
                     /product="RBS-int5"
                     /locus_tag="RBS-int5"
                     /label="RBS-int5"
                     /ApEinfo_label="RBS-int5"
                     /ApEinfo_fwdcolor="pink"
                     /ApEinfo_revcolor="pink"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    598..637
                     /product="Spacer26"
                     /locus_tag="Spacer26"
                     /label="Spacer26"
                     /ApEinfo_label="Spacer26"
                     /ApEinfo_fwdcolor="pink"
                     /ApEinfo_revcolor="pink"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    638..686
                     /product="L3S3P22"
                     /locus_tag="L3S3P22"
                     /label="L3S3P22"
                     /ApEinfo_label="L3S3P22"
                     /ApEinfo_fwdcolor="pink"
                     /ApEinfo_revcolor="pink"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    687..726
                     /product="Spacer27"
                     /locus_tag="Spacer27"
                     /label="Spacer27"
                     /ApEinfo_label="Spacer27"
                     /ApEinfo_fwdcolor="pink"
                     /ApEinfo_revcolor="pink"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    727..870
                     /product="ProC"
                     /locus_tag="ProC"
                     /label="ProC"
                     /ApEinfo_label="ProC"
                     /ApEinfo_fwdcolor="pink"
                     /ApEinfo_revcolor="pink"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    871..900
                     /product="RBS-int7"
                     /locus_tag="RBS-int7"
                     /label="RBS-int7"
                     /ApEinfo_label="RBS-int7"
                     /ApEinfo_fwdcolor="pink"
                     /ApEinfo_revcolor="pink"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    901..940
                     /product="sp28"
                     /locus_tag="sp28"
                     /label="sp28"
                     /ApEinfo_label="sp28"
                     /ApEinfo_fwdcolor="pink"
                     /ApEinfo_revcolor="pink"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    941..991
                     /product="L3S1P13"
                     /locus_tag="L3S1P13"
                     /label="L3S1P13"
                     /ApEinfo_label="L3S1P13"
                     /ApEinfo_fwdcolor="pink"
                     /ApEinfo_revcolor="pink"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    992..1031
                     /product="sp29"
                     /locus_tag="sp29"
                     /label="sp29"
                     /ApEinfo_label="sp29"
                     /ApEinfo_fwdcolor="pink"
                     /ApEinfo_revcolor="pink"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    1032..1071
                     /product="Spacer19"
                     /locus_tag="Spacer19"
                     /label="Spacer19"
                     /ApEinfo_label="Spacer19"
                     /ApEinfo_fwdcolor="pink"
                     /ApEinfo_revcolor="pink"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
ORIGIN
        1 gatactgtta cttaccgatt attgtgaaga accagaccgt ttgttgtagc acactcggcg
       61 aaaatctgaa tagtaacttc ttgacaatta atcatccggc tcgtaattta tgtggaaaag
      121 aggagaaata ctagataaag ttgtgccgta tccagcggtt accaataata gtctccaggc
      181 atcaaataaa acgaaaggct cagtcgaaag actgggcctt tcgttttatc tgttgtttgt
      241 cggtgaacgc tctctactag agtcacactg gctcaccttc gggtgggcct ttctgcgttt
      301 atatagtaaa gttccaataa gactccaggt atctgtccgt gtaaaaaaga gtattgactt
      361 cgcatctttt tgtacctata atgtgtggat cacacaggaa agtactagat tgaacctcta
      421 ctacgagtga gttgagatta cagccttact cggtaccaaa ttccagaaaa gagacgcttt
      481 cgagcgtctt ttttcgtttt ggtcccttac gctattattg aagccagtct gttaccgaag
      541 tgaaattgac ggctagctca gtcctaggta cagtgctagc cagaggaagg aggctcgaac
      601 gattacggat tgctctactg tgactgaagt ttacaaccca attattgaag gccgctaacg
      661 cggccttttt ttgtttctgg tctccctgag gcacagagat ttactttatt cacgacttca
      721 gatacgcaca gctaacacca cgtcgtccct atctgctgcc ctaggtctat gagtggttgc
      781 tggataactt tacgggcatg cataaggctc gtatgatata ttcagggaga ccacaacggt
      841 ttccctctac aaataatttt gtttaacttt agtaatttca acaaaataac taggattcga
      901 aatacggtct actacagaag ggtggtttat cttactcaac gacgaacaat aaggcctccc
      961 taacgggggg ccttttttat tgataacaaa aagatttccc tcgtcacgca gtaagtattt
     1021 atcgtagaag atcactttta tcggtttcca gaacaggtaa gagccaatag tgattacttc
     1081 gcgttatgca ggcttcctcg ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag
     1141 cggtatcagc tcactcaaag gcggtaatcc tagggcgttc ggctgcggcg agcggtatca
     1201 gctcactcaa aggcggtaat acggttatcc acagaatcag gggataacgc aggaaagaac
     1261 atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt
     1321 ttccataggc tccgcccccc tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg
     1381 cgaaacccga caggactata aagataccag gcgtttcccc ctggaagctc cctcgtgcgc
     1441 tctcctgttc cgaccctgcc gcttaccgga tacctgtccg cctttctccc ttcgggaagc
     1501 gtggcgcttt ctcaatgctc acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc
     1561 aagctgggct gtgtgcacga accccccgtt cagcccgacc gctgcgcctt atccggtaac
     1621 tatcgtcttg agtccaatgc cacctgacgt ctaagaaccc ggtaagacac gacttatcgc
     1681 cactggcagc agccactggt aacaggatta gcagagcgag gtatgtaggc ggtgctacag
     1741 agttcttgaa gtggtggcct aactacggct acactagaag gacagtattt ggtatctgcg
     1801 ctctgctgaa gccagttacc ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa
     1861 ccaccgctgg tagcggtggt ttttttgttt gcaagcagca gattacgcgc agaaaaaaag
     1921 gatctcaaga agatcctttg atcttttcta cggggtctga cgctcagtgg aacgaaaact
     1981 cacgttaagg gattttggtc atgacttgtg cttggattct caccaataaa aaacgcccgg
     2041 cggcaaccga gcgttctgaa caaatccaga tggagttctg aggtcattac tggatctatc
     2101 aacaggagtc caagcgagct cgatatcact aaatacattc aaatatctat ccgctcatga
     2161 gacaataacc ctgataaatg cttcaataat attgaaaaag gaagaatatg agtattcaac
     2221 atttccgtgt cgcccttatt cccttttttg cggcattttg ccttcctgtt tttgctcacc
     2281 cagaaacgct ggtgaaagta aaagatgccg aagatcagtt gggtgcacgt gtgggttaca
     2341 tcgaactgga cctcaacagc ggtaagattc ttgagagttt tcgccccgaa gaacgtttcc
     2401 caatgatgag cacttttaaa gttctgctct gtggcgcggt attatcccgt attgacgccg
     2461 ggcaagagca actcggtcgc cgcatacact attctcagaa tgacttggtt gagtactcac
     2521 cagtcacaga aaagcatctt acggacggca tgacagtacg cgaattatgc agcgctgcca
     2581 taaccatgag tgataacacg gcggccaact tacttctgac aacgatcgga ggaccgaagg
     2641 agcttaccgc ttttttgcac aacatgggtg atcatgtaac tcgccttgat cgttgggaac
     2701 cggagctgaa tgaagccata ccaaacgacg agcgtgacac cacgatgcct gtagctatgg
     2761 caacaacgtt gcgcaaactc ttaactggcg aacttcttac tctcgcttcc cggcaacaat
     2821 taatagactg gatggaggcg gataaagttg caggaccact tctgcgctcg gcccttccgg
     2881 ctggctggtt tattgctgat aaatctggag ccggtgagcg tgggtcccgc ggtattattg
     2941 cagccctggg gccagatggt aagccctccc gtatcgtagt tatctacacg acggggagcc
     3001 aggcaactat ggacgaacgt aatcgccaga tcgctgagat aggtgcctcc ctgattaagc
     3061 attggtaata atttagcttc cttagctcct gaaaatctcg ataactcaaa aaatacgccc
     3121 ggtagtgatc ttatttcatt atggtgaaag ttggaacctc ttacgtgccg atcaacgtct
     3181 cattttcgcc agatatcgac gtctaagaaa ccattattat catgacatta acctataaaa
     3241 ataggcgtat cacgaggccc tttcgtcttc acctcgagtg ccacctgacg tctaagaaac
     3301 cattattatc atgacattaa cctataaaaa taggcgtatc acgaggcaga atttcagata
     3361 aaaaaaatcc ttagctttcg ctaaggatga tttctgga  
//
