LOCUS       Exported                9481 bp ds-DNA     circular UNA 29-AUG-2018
DEFINITION  natural circular DNA
ACCESSION   urn.local...h-9erp7ku
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 9481)
  AUTHORS   This plasmid was generated by Dr Philipp Kaiser (NMI Tubingen)
  JOURNAL   Distributed by ChromoTek GmbH, Martinsried, Germany. 
            https://www.chromotek.com
REFERENCE   2  (bases 1 to 9481)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Aug 29, 2018 from SnapGene Viewer 4.2.4
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..9481
                     /organism="unspecified"
                     /mol_type="genomic DNA"
     misc_feature    1..6
                     /label=EcoRI
                     /note="Geneious type: restriction site"
                     /note="/Recognition_pattern=G^AATTC"
                     /note="/annotation_group=GAATTC"
     misc_feature    14..19
                     /label=BamHI
                     /note="Geneious type: restriction site"
                     /note="/Recognition_pattern=G^GATCC"
                     /note="/annotation_group=GGATCC"
     misc_feature    19..24
                     /label=AvrII
                     /note="Geneious type: restriction site"
                     /note="/Recognition_pattern=C^CTAGG"
                     /note="/annotation_group=CCTAGG"
     misc_feature    26..31
                     /label=ApaI
                     /note="Geneious type: restriction site"
                     /note="/Recognition_pattern=GGGCC^C"
                     /note="/annotation_group=GGGCCC"
     misc_feature    32..37
                     /label=HindIII
                     /note="Geneious type: restriction site"
                     /note="/Recognition_pattern=A^AGCTT"
                     /note="/annotation_group=AAGCTT"
     misc_feature    39..44
                     /label=EcoRV
                     /note="Geneious type: restriction site"
                     /note="/Recognition_pattern=GAT^ATC"
                     /note="/annotation_group=GATATC"
     misc_feature    47..52
                     /label=XhoI
                     /note="Geneious type: restriction site"
                     /note="/Recognition_pattern=C^TCGAG"
                     /note="/annotation_group=CTCGAG"
     misc_feature    54..59
                     /label=XmaI
                     /note="Geneious type: restriction site"
                     /note="/Recognition_pattern=C^CCGGG"
                     /note="/annotation_group=CCCGGG"
     misc_feature    61..66
                     /label=NheI
                     /note="Geneious type: restriction site"
                     /note="/Recognition_pattern=G^CTAGC"
                     /note="/annotation_group=GCTAGC"
     misc_feature    68..73
                     /label=SacI
                     /note="Geneious type: restriction site"
                     /note="/Recognition_pattern=GAGCT^C"
                     /note="/annotation_group=GAGCTC"
     gene            73..108
                     /label=Spot-Tag
                     /note="/Color=#ff0000"
                     /note="/Transferred_From=OTH0006_pSpot5, OTH0007_pSpot6"
                     /note="/Transferred_Similarity=100.00%"
                     /note="/X-Transferred_From=OTH0006_pSpot5"
                     /note="/X-Transferred_Similarity=100.00%"
                     /note="/created_by=c.linkewinnebeck"
     gene            109..111
                     /label=Stop
                     /note="/created_by=c.linkewinnebeck"
     promoter        complement(223..241)
                     /label=T7 prom
                     /note="/Imported_from=Pla sMapper"
                     /note="/Transferred_From=T7 prom"
                     /note="/Transferred_Similarity=100.00%"
     misc_feature    245..405
                     /label=lacZ_a reporter
                     /label=nonstandard type: Reporter
                     /note="/Imported_from=Pla sMapper"
                     /note="/Transferred_From=lacZ_a reporter"
                     /note="/Transferred_Similarity=99.38%"
     misc_feature    422..728
                     /label=f1 origin
                     /note="Geneious type: Origin of Replication"
                     /note="/Imported_from=Pla sMapper"
                     /note="/Transferred_From=f1 origin"
                     /note="/Transferred_Similarity=99.67%"
     misc_feature    1557..2663
                     /label=LEU2 marker
                     /label=nonstandard type: Marker
                     /note="/Imported_from=Pla sMapper"
                     /note="/Transferred_From=LEU2 marker"
                     /note="/Transferred_Similarity=98.55%"
     misc_feature    3389..4858
                     /label=2micron origin
                     /note="Geneious type: Origin of Replication"
                     /note="/Imported_from=Pla sMapper"
                     /note="/Transferred_From=2micron origin"
                     /note="/Transferred_Similarity=98.37%"
     misc_feature    3665..3670
                     /label=PstI
                     /note="Geneious type: restriction site"
                     /note="/Recognition_pattern=CTGCA^G"
                     /note="/annotation_group=CTGCAG"
     gene            complement(4943..4990)
                     /label=FRT
                     /note="/Imported_from=Pla sMapper"
                     /note="/Transferred_From=FRT"
                     /note="/Transferred_Similarity=100.00%"
     misc_feature    5716..6576
                     /label=amp marker
                     /label=nonstandard type: Marker
                     /note="/Imported_from=Pla sMapper"
                     /note="/Transferred_From=amp marker"
                     /note="/Transferred_Similarity=99.88%"
     rep_origin      6747..7335
                     /label=pMB1 ori
                     /note="/Imported_from=Pla sMapper"
                     /note="/Transferred_From=pBR322 origin"
                     /note="/Transferred_Similarity=99.52%"
                     /note="/modified_by=f.hartlepp"
     promoter        join(8907..9481,1..9)
                     /note="TPI1"
                     /note="/Transferred_From=OTH0006_pSpot5, OTH0007_pSpot6"
                     /note="/Transferred_Similarity=100.00%"
                     /note="/X-Transferred_From=OTH0006_pSpot5; TPI1 promoter"
                     /note="/X-Transferred_Similarity=100.00%; 98.80%"
ORIGIN
        1 gaattcacca tggggatccc taggtgggcc caagctttga tatccgctcg agccccgggt
       61 gctagcggag ctccagatag agttagagct gtttctcatt ggtcttctta actagctaac
      121 tgaataagga acaatgaacg tttttccttt ctcttgttcc tagtattaat gactgaccga
      181 tacatccctt tttttttttg tctttgtcta gctccaattc gccctatagt gagtcgtatt
      241 acaattcact ggccgtcgtt ttacaacgtc gtgactggga aaaccctggc gttacccaac
      301 ttaatcgcct tgcagcacat ccccctttcg ccagctggcg taatagcgaa gaggcccgca
      361 ccgatcgccc ttcccaacag ttgcgcagcc tgaatggcga atggacgcgc cctgtagcgg
      421 cgcattaagc gcggcgggtg tggtggttac gcgcagcgtg accgctacac ttgccagcgc
      481 cctagcgccc gctcctttcg ctttcttccc ttcctttctc gccacgttcg ccggctttcc
      541 ccgtcaagct ctaaatcggg ggctcccttt agggttccga tttagtgctt tacggcacct
      601 cgaccccaaa aaacttgatt agggtgatgg ttcacgtagt gggccatcgc cctgatagac
      661 ggtttttcgc cctttgacgt tggagtccac gttctttaat agtggactct tgttccaaac
      721 tggaacaaca ctcaacccta tctcggtcta ttcttttgat ttataaggga ttttgccgat
      781 ttcggcctat tggttaaaaa atgagctgat ttaacaaaaa tttaacgcga attttaacaa
      841 aatattaacg cttacaattt cctgatgcgg tattttctcc ttacgcatct gtgcggtatt
      901 tcacaccgca tatcgaccgg tcgaggagaa cttctagtat atctacatac ctaatattat
      961 tgccttatta aaaatggaat cccaacaatt acatcaaaat ccacattctc ttcaaaatca
     1021 attgtcctgt acttccttgt tcatgtgtgt tcaaaaacgt tatatttata ggataattat
     1081 actctatttc tcaacaagta attggttgtt tggccgagcg gtctaaggcg cctgattcaa
     1141 gaaatatctt gaccgcagtt aactgtggga atactcaggt atcgtaagat gcaagagttc
     1201 gaatctctta gcaaccatta tttttttcct caacataacg agaacacaca ggggcgctat
     1261 cgcacagaat caaattcgat gactggaaat tttttgttaa tttcagaggt cgcctgacgc
     1321 atataccttt ttcaactgaa aaattgggag aaaaaggaaa ggtgagagcg ccggaaccgg
     1381 cttttcatat agaatagaga agcgttcatg actaaatgct tgcatcacaa tacttgaagt
     1441 tgacaatatt atttaaggac ctattgtttt ttccaatagg tggttagcaa tcgtcttact
     1501 ttctaacttt tcttaccttt tacatttcag caatatatat atatatattt caaggatata
     1561 ccattctaat gtctgcccct aagaagatcg tcgttttgcc aggtgaccac gttggtcaag
     1621 aaatcacagc cgaagccatt aaggttctta aagctatttc tgatgttcgt tccaatgtca
     1681 agttcgattt cgaaaatcat ttaattggtg gtgctgctat cgatgctaca ggtgttccac
     1741 ttccagatga ggcgctggaa gcctccaaga aggctgatgc cgttttgtta ggtgctgtgg
     1801 gtggtcctaa atggggtacc ggtagtgtta gacctgaaca aggtttacta aaaatccgta
     1861 aagaacttca attgtacgcc aacttaagac catgtaactt tgcatccgac tctcttttag
     1921 acttatctcc aatcaagcca caatttgcta aaggtactga cttcgttgtt gtcagagaat
     1981 tagtgggagg tatttacttt ggtaagagaa aggaagacga tggtgatggt gtcgcttggg
     2041 atagtgaaca atacaccgtt ccagaagtgc aaagaatcac aagaatggcc gctttcatgg
     2101 ccctacaaca tgagccacca ttgcctattt ggtccttgga taaagctaat gttttggcct
     2161 cttcaagatt atggagaaaa actgtggagg aaaccatcaa gaacgagttc cctacattga
     2221 aggttcaaca tcaattgatt gattctgcca ccatgatcct agttaagaac ccaacccacc
     2281 taaatggtat tataatcacc agcaacatgt ttggtgacat catctccgat gaagcctccg
     2341 ttatcccagg ttccttgggt ttgttgccat ctgcgtcctt ggcctctttg ccagacaaga
     2401 acaccgcatt tggtttgtac gaaccatgcc acggttctgc tccagatttg ccaaagaata
     2461 aggtcaaccc tatcgccact atcttgtctg ctgcaatgat gttgaaattg tcattgaact
     2521 tgcctgaaga aggtaaggcc attgaagatg cagttaaaaa ggttttggat gcaggtatca
     2581 gaactggtga tttaggtggt tccaacagta ccaccgaagt cggtgatgct gtcgccgaag
     2641 aagttaagaa aatccttgct taaaaagatt ctcttttttt atgatatttg tacataaact
     2701 ttataaatga aattcataat agaaacgaca cgaaattaca aaatggaata tgttcatagg
     2761 gtagacgaaa ctatatacgc aatctacata catttatcaa gaaggagaaa aaggaggatg
     2821 taaaggaata caggtaagca aattgatact aatggctcaa cgtgataagg aaaaagaatt
     2881 gcactttaac attaatattg acaaggagga gggcaccaca caaaaagtta ggtgtaacag
     2941 aaaatcatga aactatgatt cctaatttat atattggagg attttctcta aaaaaaaaaa
     3001 aatacaacaa ataaaaaaca ctcaatgacc tgaccatttg atggagttta agtcaatacc
     3061 ttcttgaacc atttcccata atggtgaaag ttccctcaag aattttactc tgtcagaaac
     3121 ggccttaacg acgtagtcga tatggtgcac tctcagtaca atctgctctg atgccgcata
     3181 gttaagccag ccccgacacc cgccaacacc cgctgacgcg ccctgacggg cttgtctgct
     3241 cccggcatcc gcttacagac aagctgtgac cgtctccggg agctgcatgt gtcagaggtt
     3301 ttcaccgtca tcaccgaaac gcgcgagacg aaagggcctc gtgatacgcc tatttttata
     3361 ggttaatgtc atgataataa tggtttctta gacgtgcggc cgctctagaa ctagtggatc
     3421 aattccacgg actatagact atactagtat actccgtcta ctgtacgata cacttccgct
     3481 caggtccttg tcctttaacg aggccttacc actcttttgt tactctattg atccagctca
     3541 gcaaaggcag tgtgatctaa gattctatct tcgcgatgta gtaaaactag ctagaccgag
     3601 aaagagacta gaaatgcaaa aggcacttct acaatggctg ccatcattat tatccgatgt
     3661 gacgctgcag cttctcaatg atattcgaat acgctttgag gagatacagc ctaatatccg
     3721 acaaactgtt ttacagattt acgatcgtac ttgttaccca tcattgaatt ttgaacatcc
     3781 gaacctggga gttttccctg aaacagatag tatatttgaa cctgtataat aatatatagt
     3841 ctagcgcttt acggaagaca atgtatgtat ttcggttcct ggagaaacta ttgcatctat
     3901 tgcataggta atcttgcacg tcgcatcccc ggttcatttt ctgcgtttcc atcttgcact
     3961 tcaatagcat atctttgtta acgaagcatc tgtgcttcat tttgtagaac aaaaatgcaa
     4021 cgcgagagcg ctaatttttc aaacaaagaa tctgagctgc atttttacag aacagaaatg
     4081 caacgcgaaa gcgctatttt accaacgaag aatctgtgct tcatttttgt aaaacaaaaa
     4141 tgcaacgcga gagcgctaat ttttcaaaca aagaatctga gctgcatttt tacagaacag
     4201 aaatgcaacg cgagagcgct attttaccaa caaagaatct atacttcttt tttgttctac
     4261 aaaaatgcat cccgagagcg ctatttttct aacaaagcat cttagattac tttttttctc
     4321 ctttgtgcgc tctataatgc agtctcttga taactttttg cactgtaggt ccgttaaggt
     4381 tagaagaagg ctactttggt gtctattttc tcttccataa aaaaagcctg actccacttc
     4441 ccgcgtttac tgattactag cgaagctgcg ggtgcatttt ttcaagataa aggcatcccc
     4501 gattatattc tataccgatg tggattgcgc atactttgtg aacagaaagt gatagcgttg
     4561 atgattcttc attggtcaga aaattatgaa cggtttcttc tattttgtct ctatatacta
     4621 cgtataggaa atgtttacat tttcgtattg ttttcgattc actctatgaa tagttcttac
     4681 tacaattttt ttgtctaaag agtaatacta gagataaaca taaaaaatgt agaggtcgag
     4741 tttagatgca agttcaagga gcgaaaggtg gatgggtagg ttatataggg atatagcaca
     4801 gagatatata gcaaagagat acttttgagc aatgtttgtg gaagcggtat tcgcaatatt
     4861 ttagtagctc gttacagtcc ggtgcgtttt tggttttttg aaagtgcgtc ttcagagcgc
     4921 ttttggtttt caaaagcgct ctgaagttcc tatactttct agagaatagg aacttcggaa
     4981 taggaacttc aaagcgtttc cgaaaacgag cgcttccgaa aatgcaacgc gagctgcgca
     5041 catacagctc actgttcacg tcgcacctat atctgcgtgt tgcctgtata tatatataca
     5101 tgagaagaac ggcatagtgc gtgtttatgc ttaaatgcgt acttatatgc gtctatttat
     5161 gtaggatgaa aggtagtcta gtacctcctg tgatattatc ccattccatg cggggtatcg
     5221 tatgcttcct tcagcactac cctttagctg ttctatatgc tgccactcct caattggatt
     5281 agtctcatcc ttcaatgcta tcatttcctt tgatattgga tcatatgcat agtaccgaga
     5341 aactagtgcg aagtagtgat caggtattgc tgttatctga tgagtatacg ttgtcctggc
     5401 cacggcagaa gcacgcttat cgctccaatt tcccacaaca ttagtcaact ccgttaggcc
     5461 cttcattgaa agaaatgagg tcatcaaatg tcttccaatg tgagattttg ggccattttt
     5521 tatagcaaag attgaataag gcgcattttt cttcaaagct gcggccgcac tctcactagt
     5581 acgtcaggtg gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt atttttctaa
     5641 atacattcaa atatgtatcc gctcatgaga caataaccct gataaatgct tcaataatat
     5701 tgaaaaagga agagtatgag tattcaacat ttccgtgtcg cccttattcc cttttttgcg
     5761 gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa
     5821 gatcagttgg gtgcacgagt gggttacatc gaactggatc tcaacagcgg taagatcctt
     5881 gagagttttc gccccgaaga acgttttcca atgatgagca cttttaaagt tctgctatgt
     5941 ggcgcggtat tatcccgtat tgacgccggg caagagcaac tcggtcgccg catacactat
     6001 tctcagaatg acttggttga gtactcacca gtcacagaaa agcatcttac ggatggcatg
     6061 acagtaagag aattatgcag tgctgccata accatgagtg ataacactgc ggccaactta
     6121 cttctgacaa cgatcggagg accgaaggag ctaaccgctt ttttgcacaa catgggggat
     6181 catgtaactc gccttgatcg ttgggaaccg gagctgaatg aagccatacc aaacgacgag
     6241 cgtgacacca cgatgcctgt agcaatggca acaacgttgc gcaaactatt aactggcgaa
     6301 ctacttactc tagcttcccg gcaacaatta atagactgga tggaggcgga taaagttgca
     6361 ggaccacttc tgcgctcggc ccttccggct ggctggttta ttgctgataa atctggagcc
     6421 ggtgagcgtg ggtctcgcgg tatcattgca gcactggggc cagatggtaa gccctcccgt
     6481 atcgtagtta tctacacgac ggggagtcag gcaactatgg atgaacgaaa tagacagatc
     6541 gctgagatag gtgcctcact gattaagcat tggtaactgt cagaccaagt ttactcatat
     6601 atactttaga ttgatttaaa acttcatttt taatttaaaa ggatctaggt gaagatcctt
     6661 tttgataatc tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac
     6721 cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc
     6781 ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca
     6841 actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgttcttcta
     6901 gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct
     6961 ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg
     7021 gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc
     7081 acacagccca gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta
     7141 tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg
     7201 gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt
     7261 cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg
     7321 cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg
     7381 ccttttgctc acatgttctt tcctgcgtta tcccctgatt ctgtggataa ccgtattacc
     7441 gcctttgagt gagctgatac cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg
     7501 agcgaggaag cggaagagcg cccaatacgc aaaccgcctc tccccgcgcg ttggccgatt
     7561 cattaatgca gctggcacga caggtttccc gactggaaag cgggcagtga gcgcaacgca
     7621 attaatgtga gttagctcac tcattaggca ccccaggctt tacactttat gcttccggct
     7681 cgtatgttgt gtggaattgt gagcggataa caatttcaca caggaaacag ctatgaccat
     7741 gattacgcca agctcgaaat taaccctcac taaagggaac aaaagctggt accgggccgg
     7801 ccgtcgagct tgatggcatc gtggtgtcac gctcgtcgtt tggtatggct tcattcagct
     7861 ccggttccca acgatcaagg cgagttacat gatcccccat gttgtgcaaa aaagcggtta
     7921 gctccttcgg tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta tcactcatgg
     7981 ttatggcagc actgcataat tctcttactg tcatgccatc cgtaagatgc ttttctgtga
     8041 ctggtgagta ctcaaccaag tcattctgag aatagtgtat gcggcgaccg agttgctctt
     8101 gcccggcgtc aacacgggat aataccgcgc cacatagcag aactttaaaa gtgctcatca
     8161 ttggaaaacg ttcttcgggg cgaaaactct caaggatctt accgctgttg agatccagtt
     8221 cgatgtaacc cactcgtgca cccaactgat cttcagcatc ttttactttc accagcgttt
     8281 ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga
     8341 aatgttgaat actcatactc ttcctttttc aatattattg aagcatttat cagggttatt
     8401 gtctcatgag cggatacata tttgaatgta tttagaaaaa taaacaaata ggggttccgc
     8461 gcacatttcc ccgaaaagtg ccacctgacg tctaagaaac cattattatc atgacattaa
     8521 cctataaaaa taggcgtatc acgaggccct ttcgtcttca agaattgggg atctacgtat
     8581 ggtcatttct tcttcagatt ccctcatgga gaaagtgcgg cagatgtata tgacagagtc
     8641 gccagtttcc aagagacttt attcaggcac ttccatgata ggcaagagag aagacccaga
     8701 gatgttgttg tcctagttac acatggtatt tattccagag tattcctgat gaaatggttt
     8761 agatggacat acgaagagtt tgaatcgttt accaatgttc ctaacgggag cgtaatggtg
     8821 atggaactgg acgaatccat caatagatac gtcctgagga ccgtgctacc caaatggact
     8881 gattgtgagg gagacctaac tacatagtgt ttaaagatta cggatattta acttacttag
     8941 aataatgcca tttttttgag ttataataat cctacgttag tgtgagcggg atttaaactg
     9001 tgaggacctt aatacattca gacacttctg cggtatcacc ctacttattc ccttcgagat
     9061 tatatctagg aacccatcag gttggtggaa gattacccgt tctaagactt ttcagcttcc
     9121 tctattgatg ttacacctgg acaccccttt tctggcatcc agtttttaat cttcagtggc
     9181 atgtgagatt ctccgaaatt aattaaagca atcacacaat tctctcggat accacctcgg
     9241 ttgaaactga caggtggttt gttacgcatg ctaatgcaaa ggagcctata tacctttggc
     9301 tcggctgctg taacagggaa tataaagggc agcataattt aggagtttag tgaacttgca
     9361 acatttacta ttttcccttc ttacgtaaat atttttcttt ttaattctaa atcaatcttt
     9421 ttcaattttt tgtttgtatt cttttcttgc ttaaatctat aactacaaaa aacacataca
     9481 g
//
